Effects of Myogenin on Expression of Late Muscle Genes through MyoD-Dependent Chromatin Remodeling Ability of Myogenin

Chao Du,Ju-Hua Ni,Ya-Qiong Jin,Jun-Juan Qi,Zhen-Xing Ji,Shu-Yan Li,Guo-Shun An,Hong-Ti Jia 한국분자세포생물학회 2012 Molecules and cells Vol.34 No.2

MyoD and myogenin (Myog) recognize sets of distinct but overlapping target genes and play different roles in skeletal muscle differentiation. MyoD is sufficient for near-full expression of early targets, while Myog can only partially enhance expression of MyoD-initiated late muscle genes. However, the way in which Myog enhances the expression of MyoD-initiated late muscle genes remains unclear. Here, we examine the effects of Myog on chromatin remodeling at late muscle gene promoters and their activation within chromatin environment. Chromatin immunoprecipitation (ChIP) assay showed that Myog selectively bound to the regulatory sequences of late muscle genes. Overexpres-sion of Myog was found to overcome sodium butyrate-inhibited chromatin at late muscle genes in differ-entiating C2C12 myoblasts, shifting the transcriptional activation of these genes to an earlier time period. Furthermore, overexpression of Myog led to increased hyperacetylation of core histone H4 in differentiating C2C12 myoblasts but not NIH3T3 fibroblasts, and hyperacetylated H4 was associated directly with the late muscle genes in differentiating C2C12, indicating that Myog can induce chromatin remodeling in the presence of MyoD. In addition, co-immunopre-cipitation (CoIP) revealed that Myog was associated with the nuclear protein Brd4 in differentiating C2C12 myoblasts. Together, these results suggest that Myog enhances the expression of MyoD-initiated late muscle genes through MyoD-dependent ability of Myog to induce chromatin remodeling, in which Myog-Brd4 interaction may be involved.

Low-stiffness spring element constraint boundary condition method for machining deformation simulation

He-chuan Song,Yi-du Zhang,Qiong Wu,Han-jun Gao 대한기계학회 2020 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.34 No.10

The 3-2-1 constraint principle has been widely applied as the boundary conditions for the finite element method (FEM) to simulate machining deformation of aerospace structure components. However, this principle is inconsistent with the actual contact surface between workpieces and worktables because it provides only three constraint points. These points have rarely been studied in terms of positions and distances. In addition, the applicability of the principle is limited for the workpiece with geometric centers difficult to find or centers without nodes. Therefore, this study proposed a new boundary condition method, low-stiffness spring element constraint method (SECM), drawing on FEM theories and related mechanic theories. With the method proposed this study established the FE model of machining deformation, and then compared the simulation results with both the analytical results and the experimental results of milling and deformation measurements. Good agreement is found between the three results. Finally, this study examined the effect of the three constraint points on simulation of the 3-2-1 principle in terms of point positions and distances. A comparison between SECM and the 3-2-1 principle revealed that SECM is closer to the actual working conditions and more reliable with wider application, which suggests that SECM can replace the 3-2-1 principle as the boundary conditions for the workpiece.

SKF96365 impedes spinal glutamatergic transmission-mediated neuropathic allodynia

Qiru Wang,Yang Zhang,Qiong Du,Xinjie Zhao,Wei Wang,Qing Zhai,Ming Xiang 대한약리학회 2023 The Korean Journal of Physiology & Pharmacology Vol.27 No.1

Spinal nerve injury causes mechanical allodynia and structural imbalance of neurotransmission, which were typically associated with calcium overload. Storeoperated calcium entry (SOCE) is considered crucial elements-mediating intracellular calcium homeostasis, ion channel activity, and synaptic plasticity. However, the underlying mechanism of SOCE in mediating neuronal transmitter release and synaptic transmission remains ambiguous in neuropathic pain. Neuropathic rats were operated by spinal nerve ligations. Neurotransmissions were assessed by whole-cell recording in substantia gelatinosa. Immunofluorescence staining of STIM1 with neuronal and glial biomarkers in the spinal dorsal horn. The endoplasmic reticulum stress level was estimated from qRT-PCR. Intrathecal injection of SOCE antagonist SKF96365 dose-dependently alleviated mechanical allodynia in ipsilateral hind paws of neuropathic rats with ED50 of 18 μg. Immunofluorescence staining demonstrated that STIM1 was specifically and significantly expressed in neurons but not astrocytes and microglia in the spinal dorsal horn. Bath application of SKF96365 inhibited enhanced miniature excitatory postsynaptic currents in a dosage-dependent manner without affecting miniature inhibitory postsynaptic currents. Mal-adaption of SOCE was commonly related to endoplasmic reticulum (ER) stress in the central nervous system. SKF96365 markedly suppressed ER stress levels by alleviating mRNA expression of C/ EBP homologous protein and heat shock protein 70 in neuropathic rats. Our findings suggested that nerve injury might promote SOCE-mediated calcium levels, resulting in long-term imbalance of spinal synaptic transmission and behavioral sensitization, SKF96365 produces antinociception by alleviating glutamatergic transmission and ER stress. This work demonstrated the involvement of SOCE in neuropathic pain, implying that SOCE might be a potential target for pain management

Delivery of siRNA with Polymeric Nanoparticles for Cancer Therapy

Jun Wang,Tian-Meng Sun,Jin-Zhi Du,Cheng-Qiong Mao 한국고분자학회 2011 한국고분자학회 학술대회 연구논문 초록집 Vol.2011 No.1

Calcium-Alginate-Chitosan의 스트론튬 이온 흡착 거동

Lan Dong,Bing Deng,Lan Lan Ding,Qiong Cheng,Yong Yang,Yang Du 한국고분자학회 2014 폴리머 Vol.38 No.5

Sodium alginate and chitosan are added to a CaCl2 solution to prepare calcium-alginate-chitosan and calciumalginategels. After dehydration through stoving, two types of adsorbent particles are obtained. The adsorption processof the particles obtained for low concentrations of Sr2+ satisfies a second-order kinetic equation and the Freundlich adsorptionmodel. The thermodynamic behaviors of the particles indicate that adsorption occurs via a spontaneous physical process. XPS pattern analysis is used to demonstrate the adsorption of Sr2+ by calcium alginate and chitosan. By buildingan interaction model of the molecules of chitosan and alginate with Ca2+ and Sr2+ to calculate energy parameters, Fukuiindex, Mulliken charge, and Mulliken population, adsorption of Sr2+ on the molecular chains of chitosan as well as theboundary of calcium-alginate-chitosan is observed to show weak stability; by contrast, adsorption between molecularchains is high.

Early Efficacy of Endostar Combined with Chemoradiotherapy for Advanced Cervical Cancers

Ke, Qing-Hua,Zhou, Shi-Qiong,Huang, Min,Lei, Yong,Du, Wei,Yang, Ji-Yuan Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.3

The aim of this study was to investigate the early outcome of Endostar combined with chemoradiotherapy for advanced cervical cancer. Fifty-two cases (FIGO IIb to IVa) were divided randomly into two groups, receiving chemoradiotherapy alone (CRT group) and Endostar combined with chemoradiotherapy (CRT+E group). For the patients in the CRT+E group, Endostar was administered daily with the dosage of 7.5 $mg/m^2$, and cisplatin was administered weekly with the dosage of 20 $mg/m^2$ during the radiation. The regimens lasted for 4 weeks with no difference in chemoradiotherapy between the two groups. The early outcome complete remission rate was 73.1%, partial remission rate was 23.1% and the total response rate was 96.2% in CRT+E group, a significant improvement on the 34.6%, 42.3% and 76.9%, respectively, in the CRT group. One year survive rates were 100% and 84.6% in the CRT+E group and CRT groups, the difference being significant. Endostar combined with chemoradiotherapy can improve the early outcome of the advanced cervical cancer, and adverse effects were not encountered.

Early Efficacy of Taxotere and Cisplatin Chemo-Radiotherapy for Advanced Cervical Cancer

Ke, Qing-Hua,Zhou, Shi-Qiong,Du, Wei,Lei, Yong,Huang, Min,Luo, Fei,Yang, Ji-Yuan Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.2

The aim of this study was to investigate the early outcome of the taxotere and cisplatin chemoradiotherapy for advanced cervical cancer. Fifty-six cases (FIGO II b to IVa) were divided randomly into two groups: radiotherapy alone (28 cases) and radiation plus chemotherapy (TP) group. There was no difference in radiotherapy between the two groups. The RT+C cases who received TP regimen during the radiation, and DDP once weekly injection of vain, according to 20$mg/m^2$ and taxotere once weekly iv according to 35 $mg/m^2$. These regimens were given for 4~5weeks, and some medicines to control vomiting were available for the RT+C cases. The two groups received an oral medicine MA 160mg every day during the treatment. Regarding early outcome, the complete remission rate was 64.3% and partial remission rate was 35.7% in RT+C. The complete remission rate was 32.1% and partial remission rate was 39.3% in RT. The total response rate and complete remission in the RT+C group were higher than that in the RT group. We conclude that taxotere and cisplatin chemoradiotherapy can improve the early outcome of the advanced cervical cancer, the adverse effects being endurable.

A full path assessment approach for vibration serviceability and vibration control of footbridges

Qiankun Zhu,Xiaoli Hui,Yongfeng Du,Qiong Zhang 국제구조공학회 2019 Structural Engineering and Mechanics, An Int'l Jou Vol.70 No.6

Most of the existing evaluation criteria of vibration serviceability rely on the peak acceleration of the structure rather than that of the people keeping their own body unmoved on the structure who is the real receiver of structural vibrations. In order to accurately assess the vibration serviceability, therefore, a full path assessment approach of vibration serviceability based on vibration source, path and receiver is not only tentatively proposed in this paper, taking the peak acceleration of receiver into account, but also introduce a probability procedure to provide more instructive information instead of a single value. In fact, semi-rigid supported on both sides of the structure is more consistent with the actual situation than simply supported or clamped due to the application of the prefabricated footbridge structures. So, the footbridge is regarded as a beam with semi-rigid supported on both sides in this paper. The differential quadrature-integral quadrature coupled method is not only to handle different type of boundary conditions, but also after being further modified via the introduction of an approximation procedure in this work, the time-varying system problem caused by human-structure interaction can be solved well. The analytical results of numerical simulations demonstrate that the modified differential quadrature-integral quadrature coupled method has higher reliability and accuracy compared with the mode superposition method. What’s more, both of the two different passive control measures, the tuned mass damper and semi-rigid supported, have good performance for reducing vibrations. Most importantly, semi-rigid supported is easier to achieve the objective of reducing vibration compared with tuned mass damper in design stage of structure.

β-elemene Induces Caspase-dependent Apoptosis in Human Glioma Cells in vitro through the Upregulation of Bax and Fas/FasL and Downregulation of Bcl-2

Li, Chen-Long,Chang, Liang,Guo, Lin,Zhao, Dan,Liu, Hui-Bin,Wang, Qiu-Shi,Zhang, Ping,Du, Wen-Zhong,Liu, Xing,Zhang, Hai-Tao,Liu, Yang,Zhang, Yao,Xie, Jing-Hong,Ming, Jian-Guang,Cui, Yu-Qiong,Sun, Ying Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.23

Background: ${\beta}$-elemene, extracted from herb medicine Curcuma wenyujin has potent anti-tumor effects in various cancer cell lines. However, the activity of ${\beta}$-elemene against glioma cells remains unclear. In the present study, we assessed effects of ${\beta}$-elemene on human glioma cells and explored the underlying mechanism. Materials and Methods: Human glioma U87 cells were used. Cell proliferation was determined with MTT assay and colony formation assay to detect the effect of ${\beta}$-elemene at different doses and times. Fluorescence microscopy was used to observe cell apoptosis with Hoechst 33258 staining and change of glioma apoptosis and cell cycling were analyzed by flow cytometry. Real-time quantitative PCR and Western-blotting assay were performed to investigated the influence of ${\beta}$-elemene on expression levels of Fas/FasL, caspase-3, Bcl-2 and Bax. The experiment was divided into two groups: the blank control group and ${\beta}$-elemne treatment group. Results: With increase in the concentration of ${\beta}$-elemene, cytotoxic effects were enhanced in the glioma cell line and the concentration of inhibited cell viability ($IC_{50}$) was $48.5{\mu}g/mL$ for 24h. ${\beta}$-elemene could induce cell cycle arrest in the G0/G1 phase. With Hoechst 33258 staining, apoptotic nuclear morphological changes were observed. Activation of caspase-3,-8 and -9 was increased and the pro-apoptotic factors Fas/FasL and Bax were upregulated, while the anti-apoptotic Bcl-2 was downregulated after treatment with ${\beta}$-elemene at both mRNA and protein levels. Furthermore, proliferation and colony formation by U87 cells were inhibited by ${\beta}$-elemene in a time and does-dependent manner. Conclusions: Our results indicate that ${\beta}$-elemene inhibits growth and induces apoptosis of human glioma cells in vitro. The induction of apoptosis appears to be related with the upregulation of Fas/FasL and Bax, activation of caspase-3,-8 and -9 and downregulation of Bcl-2, which then trigger major apoptotic cascades.