Meta-analysis of the effect of melatonin application on abiotic stress tolerance in plants

Yang Xiaoxiao,Ren Jianhong,Li Juanjuan,Lin Xinyue,Xia Xiangyu,Yan Wenjie,Zhang Yuxin,Deng Xiping,Ke Qingbo 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.1

Melatonin is a hormone-like substance that promotes plant growth and development and alleviates stress levels. Although the physiological roles of melatonin and the underlying mechanisms have been qualitatively reviewed in plants, we do not fully understand when and how to apply melatonin to maximize its benefits. Here, we performed a meta-analysis to quantitatively evaluate the effect of melatonin on abiotic stress tolerance in plants and to determine the number of parameters modulated by melatonin. Melatonin significantly alleviated the growth inhibition induced by drought stress compared with other abiotic stresses, including salt, cold, heat, nitrogen deficit, and heavy metal toxicity, mainly owing to higher photosynthesis efficiency and antioxidant enzyme activity. Furthermore, melatonin modulated plant growth in a concentration-dependent manner and was more effective when applied to plants under moderate drought stress at an early stage via root irrigation. In addition, the impact of melatonin was greater in monocots than in dicots. Moreover, endogenous melatonin levels could be significantly increased via transgenic strategies. Among melatonin biosynthesis-related gene members, ASMT has tended to have the most influence on melatonin content in plants. In light of the rapidly developing genome editing technology, quantitatively increasing endogenous melatonin level in plant would be quite useful for moderating climatic conditions and combating desertification. Taken together, our results provide guidelines for melatonin application in crops plants for improving productivity under ongoing climate change.

Comparative kinetic study of coal gasification with steam and CO2 in molten blast furnace slags

Fan Yang,Qingbo Yu,Huaqing Xie,Zongliang Zuo,Limin Hou,Qin Qin 한국화학공학회 2018 Korean Journal of Chemical Engineering Vol.35 No.8

To make a comparison between coal gasification in molten blast furnace slag (MBFS) in different ambience and choose an appropriate agent to recover BF slag’s waste heat entirely, coal gasification with steam and CO2 in molten blast furnace slags was studied by isothermal thermo-gravimetric analysis. The effects of temperature and addition of MBFS were studied. Carbon conversion and reaction rate increased with increasing temperature and MBFS. Volumetric model (VM), shrinking core model (SCM), and diffusion model (DM) were applied to describe the coal gasification behavior of FX coal. The most appropriate model describing the coal gasification was SCM in steam ambience and VM in CO2 ambience, respectively. The reaction rate constant k(T) in CO2 ambience is greater than that in steam ambience, which means the gasification reactivity of coal in CO2 ambience is better than that in steam ambience. BF slag can effectively reduce the activation energy EA of coal gasification reaction in different ambiences. But, the difference of activation energies is not large in different ambiences. Based on the results of kinetic analysis including k(T) and EA calculated by the established model, CO2 was chosen to be the most appropriate agent.

Bioactive polysaccharides isolated from the fruiting bodies of Phellinus sp.

Yan Yang,Jingsong Zhang,Yanfang Liu,Qingbo Wang,Qingjiu Tang,Shuai Zhou 한국버섯학회 2010 한국버섯학회 국제학술대회 Vol.6 No.-

Bioactive polysaccharides isolated from the fruiting bodies of Phellinus sp.

Yan Yang,Jingsong Zhang,Yanfang Liu,Qingbo Wang,Qingjiu Tang,Shuai Zhou 한국버섯학회 2010 한국버섯학회지 Vol.8 No.4

Phellinus sp. are assigned to the Basidiomycotina, Hymenomycetes, Aphyllophorales and Hymenochaetaceae, and have been shown containing various bioactive substances including triterpenoids, polysaccharides and flavones[1]. Traditional Chinese herbalists believe that Phellinus species are effective in treating many gynecopathic ailments[2] and are also reported to exhibit other pharmacological functions including tumor cell inhibition, antioxidant activity and anti-hepatic fibrosis effects[3]. Polysaccharides of Phellinus sp. has been reported to possess antitumor activities and inhibit tumor recrudescence and metastasis. There are little studies comparing the chemical composition and biological activities difference among polysaccharides from different Phellinus sp and little report about the pure polysaccharide structure analysis. In this study, eight kinds of crude polysaccharides were extracted from Phellinus fruit bodies and their chemical composition and bioactivities were researched. The polysaccharide and protein contents of eight crude polysaccharides had a certain extent differences. Monosaccharide composition and content of amino acids also existed some differences in eight crude polysaccharides. Eight different polysaccharides all showed enhancing splenocyte proliferation effect in vitro. PB-10P and JSHP had high cell proliferation rates with 50㎍/ml concentrations. The results indicated in some extent the immune activity of crude polysaccharides were correlation with the polysaccharide and protein content and composition of each sample. The crude polysaccharides of P. igniarius were further isolated and purified using DEAE Sepharose F. F. and gel-filtration chromatography (Sephacryl S-100-500 )repeatedly. Five water-soluble homogeneous polysaccharides (P60w1、P60s1、P1SP1、P10SP1and P100SP1) were obtained. Lack of absorption at 280 nm and 260 nm by UV scanning indicated that contained no protein and nucleic acid. HPLC produced a single symmetrical peak, indicated homogeneity and their molecular weigh were 1.71×104 Da、2.07×104 Da、1.48×104 Da、2.20×104 Da and 2.56×104 Da respectively. Structural of P60w-1 were determined using sugar and methylation analysis combined with 1H and 13C NMR spectroscopy, including COSY, TOCSY, NOESY, HSQC and HMBC experiments. The effect of P60w-1 on tumor growth was examined using subcutaneously transplanted H22 and Lewis Lung Carcinoma (LLC) tumor mouse models. Cyclophosphamide or Coriolus versicolor polysaccharopeptide served as positive controls in evaluating tumor response. Results showed that P60w-1 at the most effective dose of 100 mg/kg inhibited the growth of H22 and LLC by 48% and 37%, respectively.

Sensitivity analysis of key factors in controlling absorption and desorption of oxygen to oxygen carriers

Limin Hou,Qingbo Yu,Kun Wang,Shuo Zhang,Qin Qin,Fan Yang 한국화학공학회 2019 Korean Journal of Chemical Engineering Vol.36 No.1

Chemical looping air separation gives an oxygen resource for the oxy-fuel combustion system. To investigate the sensitivity of operation parameters and optimal operation parameters, with the consideration of the reactor temperature, we used the oxygen concentration, and reaction gas flow, an orthogonal experiment and multi-objective comprehensive evaluation method to analyze the results obtained by fixed-bed apparatus with the YBaCo4O7+, Y0.95Ti0.05BaCo4O7+, Y0.5Dy0.5BaCo4O7+, and Y0.2Ti0.05Dy0.75BaCo4O7+ oxygen carriers. The results showed that the effects of operating conditions on oxygen absorption/desorption properties varieds in the order: oxygen concentration>gas flow rate>absorption temperature=desorption temperature. Analysis of max-min difference showed that the optimum operating conditions such as absorption temperature, 350 oC, desorption temperature, 430 oC, gas flow rate, 200 ml/min, and oxygen concentration, 21% were confirmed

Optimization of dyeing parameters of cotton standardized samples for laundry test of dye transfer inhibition program

Mingqi Guo,Li Jiang,Qingbo Yang,Chang Sun,Jianli Liu,Weidong Gao 한국의류학회 2020 Fashion and Textiles Vol.7 No.1

In order to prevent light-colored clothes from being stained by dyes released from dark clothes during the washing process, some new-type washing machines have developed the dye transfer inhibition washing program. However, there is no certified reference materials for the test of dye transfer inhibition function. To this end, cotton fabric and reactive dyes are used as experimental materials to prepare standardized samples to evaluate the dye transfer inhibition function of washing machines. Firstly, the single factor analysis method is used to analyze the significance of the dyeing parameters including dye dosage, dyeing temperature, sodium sulfate dosage and sodium carbonate dosage. Secondly, a 4-factor 5-level experimental design and theoretical prediction of the best dyeing parameters are successively carried out through central composite design and response surface method. Two evaluation indicators, the dye release amount of the standardized sample of dyed fabric and the color difference value of the standardized sample of white fabric after washing, are proposed as the response values for response surface analysis to search the optimal dying parameters in theory. The optimal dyeing parameters obtained through response surface analysis are that the dosage of dye is 5.63% (owf), the dyeing temperature is 60 °C, the dosage of sodium sulfate is 93.60 g/L, and the dosage of sodium carbonate is 15 g/L. Experimental results indicate that the standardized samples prepared with optimal dyeing parameters can effectively distinguish the dye transfer inhibition function of washing machines.

The effect of washing parameters on the quantity of dye discharge from clothes

Xu Zhao,Ming Xiong,Li Jiang,Qingbo Yang,Cunling Zhou,Jianli Liu 한국의류학회 2022 Fashion and Textiles Vol.9 No.1

In this paper, the efect of washing parameters on the quantity of dye discharge from fabric dyed in highly saturated color (FSC) was discussed. Firstly, the FSC was prepared with reactive red 195 to represent the dark clothes. Secondly, the efect of the washing parameters, including washing time, washing temperature, washing water volume, the weight of FSC, and the dosage of detergent, on dye discharge was analyzed through the single factor test. Thirdly, the mathematical relationship between the quantity of dye discharge (DDQ) and the four washing parameters was discussed through the center combination experiment and response surface method. And a quadratic model was built to predict the quantity of dye discharge when the FSC were washed with diferent washing parameters. The experimental results indicated that the quantity of dye discharge was afected by the four selected washing parameters and the optimal washing parameters to reduce the quantity of dye discharge can be deduced from the quadratic model. The optimized parameters efectively reduced 0.99 mg dye discharge per liter, and the DDQ reduction rate was up to 53.5%.

Magnetic field controlled graphene oxide-based origami with enhanced surface area and mechanical properties

Park, Ok-Kyung,Tiwary, Chandra Sekhar,Yang,Bhowmick, Sanjit,Vinod, Soumya,Zhang, Qingbo,Colvin, Vicki L.,Asif, S. A. Syed,Vajtai, Robert,Penev, Evgeni S.,Yakobson, Boris I.,Ajayan, Pulickel M. The Royal Society of Chemistry 2017 Nanoscale Vol.9 No.21

Changes in plant anthocyanin levels in response to abiotic stresses: a meta-analysis

Yan Wenjie,Li Juanjuan,Lin Xinyue,Wang Lina,Yang Xiaoxiao,Xia Xiangyu,Zhang Yuxin,Yang Shaoyu,Li Hongbing,Deng Xiping,Ke Qingbo 한국식물생명공학회 2022 Plant biotechnology reports Vol.16 No.5

Anthocyanins are small molecule antioxidants that play important roles in plant response and resistance to abiotic stresses. Their levels increase when plants are exposed to abiotic stress. However, the general response patterns and magnitude of anthocyanin increase, and how they confer resistance to abiotic stresses, are difficult to evaluate because of the influence of experimental variables. In this study, changes in plant anthocyanin content under different abiotic stresses and the effect of anthocyanin overproduction on various physiological indicators were investigated through meta-analysis derived from 1039 datasets of 102 studies. Results showed that among the different stress types, heavy metals, especially copper (Cu) and mercury (Hg), induced the most significant synthesis of anthocyanins. Among the different types of drought treatments, mannitol caused anthocyanin content in plants to increase by 2.4-fold, which was more significant than that of polyethylene glycol and natural drought. Furthermore, UV stress led plant anthocyanins to increase 1.3-fold in C4 plants, which was higher than that in C3 plants. In addition, anthocyanins increased the most when the applied stresses were low and moderate, and of short duration. Moreover, plants overexpressing genes encoding MYB transcription factors increased anthocyanin content by 5.8-fold and significantly improved plant stress tolerance. Among the 21 physiological indicators, glutathione and proline levels increased the most in transgenic plants overproducing anthocyanins when exposed to abiotic stress. Taken together, this meta-analysis indicates that anthocyanins enhance stress tolerance by improving the antioxidant, metal-chelating, and osmoregulatory abilities of the plant. The results presented here can help guide future applications of anthocyanins as plant growth regulators in dryland agriculture and breeding for plant stress tolerance.