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Enhancement of pradimicin production in Actinomadura hibisca P157-2 by metabolic engineering
Paudel, S.,Lee, H.C.,Kim, B.S.,Sohng, J.K. G. Fischer 2011 MICROBIOLOGICAL RESEARCH Vol.167 No.1
Actinomadura hibisca P157-2 produces potent antifungal antibiotic pradimicins. To enhance pradimicin production, ACCase from Streptomyces coelicolor and two regulatory genes metK1-sp and afsR-sp from Streptomyces peuticus were overexpressed into A. hibisca using an integration vector pSET152 under the control of the strong ermE* promoter. The constructed plasmids pACC152, pSAM152, pAFS152, pSA152 and pASA152 were transformed into A. hibisca by the conjugal method. The recombinant strains A. hibisca ACC, A. hibisca SAM, A. hibisca AFS, A. hibisca SA and A. hibisca ASA produced greater amounts of pradimicin than the parental strain with an increment of 3-fold, 2.1-fold, 2.8-fold, 3.4-fold, and 4.5-fold respectively. To increase the acetyl-coA pool, the strains were fed methyl oleate and acetate as carbon sources. The production was increased in wild-type A. hibisca, A. hibisca ACC and A. hibisca ASA by 2.2-fold, 4.12-fold and 5.98-fold respectively, with oleate and by 1.12-fold, 3.8-fold and 5.38-fold respectively with acetate. The strain A. hibisca ASA remained the best strain for the production of pradimicin. The higher transcriptional levels of structural genes in the strains harboring metK1-sp and afsR-sp compared to the wild-type strain were consistent with the enhanced production.
( Paudel Dinesh ) 한국운동역학회 2021 한국운동역학회지 Vol.31 No.1
Objective: The goal of this study is to calculate and compare the Maximum Lyapunov Exponent (MLE) for the anteroposterior, mediolateral and vertical displacement of the markers attached to bony land marks of the trunk and foot. Method: Ten young and healthy male subjects (age: 26.5±3.27 years, height: 167.44±5.12 cm, and weight 69.5±7.36) participated in the study. Three-dimensional positional coordinate of eight different trunk and foot marker during walking on tread mill were analysed. Results: MLE values for anteroposterior displacement of the marker were found to be significantly different with MLE values for mediolateral and vertical displacement whereas MLE values for mediolateral displacement of the marker shows no significant difference with the MLE values for vertical displacement of the markers at significance level 0.05. Conclusion: Finding of this study suggest that it is essential to consider the displacement in all three direction to examine the real characteristic of a gait signal.
( Paudel Dinesh ),( Jin-ho Back ) 한국운동역학회 2021 한국운동역학회지 Vol.31 No.3
Objective: The purpose of this study is to investigate whether the athletic knee show greater rotation and translation movement than non-athletic knee during the treadmill walking with their preferred speed in a complete gait cycle. Method: Thirty young and healthy male subjects participated in the study, fifteen handball players (mean age: 19.6 ± 1.4 years old, mean weight: 85 ± 11.9 Kg, mean height: 179.8 ± 4.7) and fifteen non-athletes (mean age: 22.8 ± 1.2 years old, mean weight: 74.5 ± 8.6 Kg, mean height: 175 ± 5.9). Three-dimensional positional coordinate of lower limb during treadmill walking were analyzed. Results: There were significant differences (t (22.014)=1.585, p=0.127 in the range of internal and external rotation with mean value for handball player (M=14.4513, SD=2.3839) was higher than non-athletes (M= 13.3327, SD=1.337). The magnitude of the difference in the means (mean difference=1.11867, 95% CI: -0.34489 to 2.5822) was significant. There were also significant differences (t (17.956)=1.654, p=0.116 in the max abduction and adduction with mean value for handball player (M=5.7160, SD=2.49281) was higher than non-athletes (M=4.5773, SD=0.94667). The magnitude of the difference in the means (mean difference=1.138, 95% CI: -0.30805 to 2.58539) was significant. At significance level 0.05. Conclusion: Finding of this study suggest that to understand the actual characteristic of knee motion studies have to be done in different walking and running trial at variable speed.
Paudel Suresh,Wang Shuji,Kim Eunae,Kundu Dooti,Min Xiao,Shin Chan Young,Kim Kyeong-Man 한국응용약물학회 2022 Biomolecules & Therapeutics(구 응용약물학회지) Vol.30 No.2
Tetrazoles were designed and synthesized as potential inhibitors of triple monoamine neurotransmitters (dopamine, norepinephrine, serotonin) reuptake based on the functional and docking simulation of compound 6 which were performed in a previous study. The compound structure consisted of a tetrazole-linker (n)-piperidine/piperazine-spacer (m)-phenyl ring, with tetrazole attached to two phenyl rings (R1 and R2). Altering the carbon number in the linker (n) from 3 to 4 and in the spacer (m) from 0 to 1 increased the potency of serotonin reuptake inhibition. Depending on the nature of piperidine/piperazine, the substituents at R1 and R2 exerted various effects in determining their inhibitory effects on monoamine reuptake. Docking study showed that the selectivity of tetrazole for different transporters was determined based on multiple interactions with various residues on transporters, including hydrophobic residues on transmembrane domains 1, 3, 6, and 8. Co-expression of dopamine transporter, which lowers dopamine concentration in the biophase by uptaking dopamine into the cells, inhibited the dopamine-induced endoctytosis of dopamine D2 receptor. When tested for compound 40 and 56, compound 40 which has more potent inhibitory activity on dopamine reuptake more strongly disinhibited the inhibitory activity of dopamine transporter on the endocytosis of dopamine D2 receptor. Overall, we identified candidate inhibitors of triple monoamine neurotransmitter reuptake and provided a theoretical background for identifying such neurotransmitter modifiers for developing novel therapeutic agents of various neuropsychiatric disorders.
Paudel Padam Prasad,Pokhrel Dharma Raj,Koirala Sajan,Baitha Lalan,김대현,Kafle Sagar 한국농업기계학회 2021 바이오시스템공학 Vol.46 No.1
Purpose We investigated the energy usage, economics, and global warming potential (GWP) of spring rice production via farm sizes in Nepal. Methods Seventy farmers were selected via purposive sampling methods, and data were collected by interviewing the farmers on site. Results It was found that 22,987 mega joules of energy per hectare (MJ/ha) was invested in order to yield 77,355 MJ/ha worth of outputs (5262 kg/ha). The analysis shows that the highest share of energy input was contributed by machinery (33.50%) and least by chemicals (0.96%). It was further found that spring rice farming in Nepal is less energy productive (0.23 kg/MJ) and less energy efficient (3.37) than that of neighboring countries—such as India, China, and Pakistan. The total GWP is estimated about 720.56 kgCO2eq/ha. The average cost of productionwas found to equate to USD810.24/ha (or USD0.154/kg of production), and that profit only reached USD73.93/ha. Conclusion This study found that energy use, cost of production, and yield per ha all generally decrease as the size of the landholding increases, whereas energy use efficiency and profits increase to an optimum level of land size and inputs. Low profits could be the result of the small size of landholdings and low levels of mechanization. This can be improved by increasing energy inputs and practicing community-farming.