Differential regulation of p53 and p21 by MKRN1 E3 ligase controls cell cycle arrest and apoptosis.

Lee, Eun-Woo,Lee, Min-Sik,Camus, Suzanne,Ghim, Jaewang,Yang, Mi-Ran,Oh, Wonkyung,Ha, Nam-Chul,Lane, David P,Song, Jaewhan Published for the European Molecular Biology Organ 2009 The EMBO journal Vol.28 No.14

<P>Makorin Ring Finger Protein 1 (MKRN1) is a transcriptional co-regulator and an E3 ligase. Here, we show that MKRN1 simultaneously functions as a differentially negative regulator of p53 and p21. In normal conditions, MKRN1 could destabilize both p53 and p21 through ubiquitination and proteasome-dependent degradation. As a result, depletion of MKRN1 induced growth arrest through activation of p53 and p21. Interestingly, MKRN1 used earlier unknown sites, K291 and K292, for p53 ubiquitination and subsequent degradation. Under severe stress conditions, however, MKRN1 primarily induced the efficient degradation of p21. This regulatory process contributed to the acceleration of DNA damage-induced apoptosis by eliminating p21. MKRN1 depletion diminished adriamycin or ultraviolet-induced cell death, whereas ectopic expression of MKRN1 facilitated apoptosis. Furthermore, MKRN1 stable cell lines that constantly produced low levels of p53 and p21 exhibited stabilization of p53, but not p21, with increased cell death on DNA damage. Our results indicate that MKRN1 exhibits dual functions of keeping cells alive by suppressing p53 under normal conditions and stimulating cell death by repressing p21 under stress conditions.</P>

Plenary Lecture : New isoforms of p53 in cncer and development

( Sir David P. Lane ) 한국생화학분자생물학회 (구 한국생화학회) 2007 생화학분자생물학회 춘계학술발표논문집 Vol.2007 No.-

Occurrence of a chiral-like pair band and a six-nucleon noncollective oblate isomer in ¹²⁰I

Moon, B.,Moon, C.-B.,Dracoulis, G.D.,Bark, R.A.,Byrne, A.P.,Davidson, P.A.,Lane, G.J.,Kibé,di, T.,Wilson, A.N.,Yuan, C.,Hong, B. Elsevier 2018 Physics letters: B Vol.782 No.-

<P><B>Abstract</B></P> <P>We report for the first time two distinctive features in the odd–odd nucleus <SUP>120</SUP>I: a pair of doublet bands and a high-spin isomer built on the π <SUB> h 11 / 2 </SUB> ν <SUB> h 11 / 2 </SUB> configuration. For producing the excited states of <SUP>120</SUP>I, a fusion-evaporation reaction <SUP>118</SUP>Sn(<SUP>6</SUP>Li, 4n) at E = l a b 48 MeV was employed. The beam was provided by the 14UD tandem accelerator of the Heavy Ion Accelerator Facility at the Australian National University. The observed doublet structure built on the positive-parity states is the first case and unique in isotopes with Z = 53 . The emerging properties are indicative of the known chiral characteristics, leading to a doubling of states for the π <SUB> h 11 / 2 </SUB> ν <SUB> h 11 / 2 </SUB> configuration. In contrast, the high-spin isomer with a half-life of 49(2) ns at spin-parity <SUP> J π </SUP> = <SUP> 25 + </SUP> can be explained in terms of a noncollective oblate structure with the full alignment of six valence nucleons outside the <SUP>114</SUP>Sn core: three protons <SUP> ( <SUB> g 7 / 2 </SUB> ) 1 </SUP> <SUP> ( <SUB> d 5 / 2 </SUB> ) 1 </SUP> <SUP> ( <SUB> h 11 / 2 </SUB> ) 1 </SUP> and three neutrons <SUP> ( <SUB> h 11 / 2 </SUB> ) 3 </SUP> . This is an outstanding case that reveals a pure single-particle structure consisting of equal numbers of valence protons and neutrons outside the semi-double shell closure of <SUP>114</SUP>Sn with Z = 50 and N = 64 .</P>

Lymphoid tissue inducer cells: architects of CD4 immune responses in mice and men

Kim, M.-Y.,Kim, K.-S.,McConnell, F.,Lane, P. Blackwell Publishing Ltd 2009 Clinical and experimental immunology Vol.157 No.1

<P>Summary</P><P>In this review, we summarize the current understanding of the multiple functions of the mouse lymphoid tissue inducer (LTi) cells in: (i) the development of organized lymphoid tissue, (ii) the generation and maintenance of CD4-dependent immunity in adult lymphoid tissues; and (iii) the regulation of central tolerance in thymus. By contrast with mouse LTi cells, which have been well described, the human equivalent is only just beginning to be characterized. Human LTi-like cells expressing interleukin (IL)-22 have been identified recently and found to differentiate into natural killer (NK) cells. The relationship of LTi cells to NK cells is discussed in the light of several studies reporting a close relationship in the mouse between LTi cells and transcription factor retinoid-related orphan receptor &ggr;t-dependent IL-22 producing NK cells in the gut. We also outline our data suggesting that these cells are present in adult human lymphoid tissues.</P>

수도사업의 방식

Westerhoff, Garret P.,Lane, Thomas J . 한국수처리기술연구회 1996 한국수처리학회지 Vol.4 No.2

수처리시설들이 더나은 수질과 감소된 비율과 가격에 서비스를 제공함으로써 더욱 경쟁적으로 되는데 직면해 있다. 이것을 만족하기 위해 미국 산업의 민간부분에서 과거에 참여해 왔던 이러한 것과 비슷한 reengineering techniques의 접목이 요구되어진다. reengineering 동안 소비자에게 좀더 나은 수질을 제공해왔다는 지난 오년동안의 전통적인 촛점을 잃지 않는 것이 중요하다. O&M에서와 수처리시설의 소유에서 민간부분 참여가 증가되어지는 것이 경쟁을 받아들인다는 뜻으로서 엄청나 주의를 끌고있다. 민간부분 연계가 소유, 경영 그리고 시설의 운전 모든 부문에서 특별화된 서비스를 외적요인으로부터 수용할 수 있다. 또한 경쟁은 reengineering 조직과 경영구조를 제외한 공공의 소유와 운용을 지속함으로써 얻어진다. 이것은 민간부분 운용에 의해 얻어질 수 있는 성과에 부합한 모든 성과를 얻고 공공보건에 높은 수준을 제공하고 소비자 서비스와 가격하락을 개선하기 위해 위임하는 접목과 유지관리를 요구한다. 외적요인의 몇가지는 경쟁을 증가 시키기위해 전술의 부분이다. 수처리는 언제나 이 방향으로 흐르고 있다. 문제에 하나의 해답만이 있을 수 없다. "무엇이 최선인가?" 몇몇은 수처리시설이 미래의 최고 대안으로서 민간부분 연계를 지지한다. 가장 건전한 상황은 산업에서 존재하기 위해 모두 앞서는 것 일것이고 경쟁을 제공하는 것이다. 각 공무원에게 무엇이 최고인지가 달여있다.

Simulation of Interferometric Noise Appearing in Networks Carring Optically Generated Millimeter-Wave Signals

M. Darby,L. Moura,P. Lane,J. J. O'reilly 한국통신학회 1997 OPTOELECTRONICS & COMMUNICATIONS CONFERENCE Vol.2 No.1

Decay Schemes of Three-Quasiparticle Isomers in ^(119,121)Sb and ^(121,123)I

C.-B. Moon,G. D. Dracoulis,R. A. Bark,A. P. Byrne,P. A. Davidson,T. Kibedi,G. J. Lane,A. N. Wilson 한국물리학회 2011 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.59 No.23

The gamma-ray decay of isomers in near spherical ^(119,121)Sb and transitional ^(121,123)I have been studied using γ-ray spectroscopy with ^7Li pulsed beams delivered from 14 UD Pelletron accelerator at the Australian National University. Isomers of J^π = 25/2^+, 19/2^- and 21/2^- with half-lives of a few nanoseconds to hundreds microseconds have been observed at 2.4 < E_x < 2.9 MeV. Decay schemes of the isomers in these nuclei are presented and their properties are interpreted in terms of three quasiparticle configurations based on the two-neutron (h_(11/2))_2 10^+ and (d_(3/2)h_(11/2)) 7^- states in even-A Sn isotones coupled to an extra proton.

Collective Bands Built on the Proton h11/2 and the Neutron h11/2 Orbitals in Odd-odd I

C.-B. Moon,G. D. Dracoulis,R. A. Bark,A. P. Byrne,P. A. Davidson,A. N. Wilson,A. M. Baxter,T. Kib edi,G. J. Lane 한국물리학회 2003 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.43 No.I

The excited states of the doubly-odd 124I, 122I, and 120I nuclei have been studied by using in-beam gamma-ray spectroscopy with the 122Sn(7Li,5n), the 120Sn(7Li,5n), and the 118Sn(6Li,4n) reactions at Elab = 54, 58, and 48 MeV, respectively. Beams, which were pulsed on 1 ns separated by 1.7 s, were provided by the 14UD Pelletron accelerator at the Australian National University. In the present work, many collective bands with positive-parity as well as negative-parity have been newly identied and their excitation energies have been denitely established. The positive-parity collective bands built on the 10+ state in these doubly odd I could be interpreted as being associated with the h11=2h11=2 conguration.

Various Isomers in Doubly Odd I Isotopes

C.-B. Moon,G. D. Dracoulis,R. A. Bark,A. P. Byrne,P. A. Davidson,T. Kibedi,G. J. Lane,A. N. Wilson 한국물리학회 2011 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.59 No.23

Isomeric states (Isomers) in odd-odd ^(120)I, ^(122)I, and ^(124)I nuclei have been investigated using in-beam gamma-ray spectroscopy with the ^(118)Sn (^6Li, 4n), ^(120)Sn (^7Li, 5n), and ^(122)Sn (^7Li, 5n) reactions at E_(lab) = 48, 58, and 54 MeV, respectively. Several isomers with half-lives ranging from a few nanoseconds to tens of microseconds have been identified for the first time and the absolute excited energies have been established by linking the low- and high-spin levels through the feeding and decay of the isomers in these nuclei. The properties of the isomers have been discussed in terms of spin traps and high-K isomerism based on two-quasiparticle configurations. The systematics for the absolute excitation energies of the J^π = 7^- and 8^- high-K isomers based on the πg_(9/2) υh_(11/2) configuration are presented for ^(116)I to ^(124)I and compared with the 9/2^+ states in the neighboring odd-A I isotopes.

Skin Models to Study Common and Rare Diseases

( John E Acommon ),( Christabelle S M Goh ),( Declan P Lunny ),( Mark B Y Tang ),( E Birgitte Lane ) 한국피부장벽학회 2014 한국피부장벽학회지 Vol.16 No.2

Use of human tissues for research is a sensitive and heavily legislated issue in developed countries. As drug discovery and human trials become increasingly expensive and produce fewer marketable drugs, the need for good pre-clinical tissue culture models for validating therapeutic approaches and early toxicity testing is becoming acute. Here in IMB we are therefore investing in the development of tissue culture models for genetic diseases. We are focussing on two diseases, one very rare and one very common, both diseases in which the major causative mutations are well known, for tissue culture model development. On the one hand epidermolysis bullosa simplex (EBS) is a very rare genetic skin blistering disorder, caused by mutations in the keratins expressed in the epidermal basal layer, i.e. keratins K5 and K14. These are compact genes and the disease-causing mutations are generally dominant, meaning only one copy is usually mutated. In contrast, atopic dermatitis (AD) or eczema is a common skin disorder characterised by dry, itchy and inflamed skin that affects up to 20% of people in developed countries. Mutations in filaggrin occur in up to 40% of AD patients, making defects in this gene by far the largest risk factor for AD. Thus the two diseases present very different challenges in generating a culture model of disease.