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Ozasa, Kazunari,Won, June,Song, Simon,Maeda, Mizuo Elsevier 2018 Applied soft computing Vol.70 No.-
<P><B>Abstract</B></P> <P>We developed a bio-inspired neurocomputing approach based on our earlier biological neurocomputer, which leverages the survival strategies of living micro-algae cells (<I>Euglena gracilis</I>) to soft computing. Instead of using the real living cells, the bio-inspired neurocomputing in this study (namely, <I>Euglena</I>-inspired neurocomputing) mimics the photophobic responses of the cells using photo-responsive (PR) noise oscillators. The PR noise oscillators play the role of neurons during computation and their output signals are autonomously changed both by noise generation and firing of the neuron. The <I>Euglena</I>-inspired neurocomputing achieved a high performance in searching for multiple solutions continuously and autonomously for a combinatorial optimization problem, 16-city TSP as instance. We analyzed the temporal evolution of the computation and its dependence on the parameter set of the PR noise oscillators and identified the source of the high performance as the trade-off between noise amplitude and the reduction ratio of the oscillators. We next introduced two specific survival strategies observed in the real <I>Euglena</I> cells to PR noise oscillators, and elucidated their positive effects on the performance. The <I>Euglena</I>-inspired neurocomputing examined in this study can be used to address dynamically changing optimization problems, since the computation tracks changes in the imposed conditions by autonomous and non-converged searching for the solutions.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Bio-inspired neurocomputing with software noise oscillators that mimic the photophobic behaviors of microalgae cells of <I>Euglena gracilis</I>. </LI> <LI> Advanced study from <I>Euglena</I>-cell-based neurocomputing we published in Appl. Soft Comput. [K. Ozasa et al., Appl. Soft Comput., 13 (2013) 527–538]. </LI> <LI> Taking the spontaneous behaviors and the survival strategies of the real living cells into neurocomputing. </LI> <LI> Dynamic transition among best or 2nd best solutions in a 16-city TSP combinatorial optimization. </LI> <LI> Focusing on the elucidation of the origin of the relatively good performance in <I>Euglena</I>-inspired neurocomputing. </LI> </UL> </P>
Ozasa, Kazunari,Lee, Jeesoo,Song, Simon,Hara, Masahiko,Maeda, Mizuo MIT Press 2015 Artificial life Vol.21 No.2
<P>Artificial linking of two isolated culture dishes is a fascinating means of investigating interactions among multiple groups of microbes or fungi. We examined artificial interaction between two isolated dishes containing Euglena cells, which are photophobic to strong blue light. The spatial distribution of swimming Euglena cells in two micro-aquariums in the dishes was evaluated as a set of new measures: the trace momentums (TMs). The blue light patterns next irradiated onto each dish were deduced from the set of TMs using digital or analogue feedback algorithms. In the digital feedback experiment, one of two different pattern-formation rules was imposed on each feedback system. The resultant cell distribution patterns satisfied the two rules with an and operation, showing that cooperative interaction was realized in the interlink feedback. In the analogue experiment, two dishes A and B were interlinked by a feedback algorithm that illuminated dish A (B) with blue light of intensity proportional to the cell distribution in dish B (A). In this case, a distribution pattern and its reverse were autonomously formed in the two dishes. The autonomous formation of a pair of reversal patterns reflects a type of habitat separation realized by competitive interaction through the interlink feedback. According to this study, interlink feedback between two or more separate culture dishes enables artificial interactions between isolated microbial groups, and autonomous cellular distribution patterns will be achieved by correlating various microbial species, despite environmental and spatial scale incompatibilities. The optical interlink feedback is also useful for enhancing the performance of Euglena-based soft biocomputing.</P>
Ozasa, Kazunari,Lee, Jeesoo,Song, Simon,Hara, Masahiko,Maeda, Mizuo Royal Society of Chemistry 2011 Lab on a chip Vol.11 No.11
<P>We examined two-dimensional (2D) optical feedback control of phototaxis flagellate <I>Euglena</I> cells confined in closed-type microfluidic channels (microaquariums), and demonstrated that the 2D optical feedback enables the control of the density and position of <I>Euglena</I> cells in microaquariums externally, flexibly, and dynamically. Using three types of feedback algorithms, the density of <I>Euglena</I> cells in a specified area can be controlled arbitrarily and dynamically, and more than 70% of the cells can be concentrated into a specified area. Separation of photo-sensitive/insensitive <I>Euglena</I> cells was also demonstrated. Moreover, <I>Euglena</I>-based neuro-computing has been achieved, where 16 imaginary neurons were defined as <I>Euglena</I>-activity levels in 16 individual areas in microaquariums. The study proves that 2D optical feedback control of photoreactive flagellate microbes is promising for microbial biology studies as well as applications such as microbe-based particle transportation in microfluidic channels or separation of photo-sensitive/insensitive microbes.</P> <P>Graphic Abstract</P><P>We developed and demonstrated 2D optical feedback control of the density and position of <I>Euglena</I> cells swimming in microaquariums. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c0lc00719f'> </P>
小笹克行(Ozasa, Katsuyuki) 대한일어일문학회 2014 일어일문학 Vol.61 No.-
Comparing the Korean university courses related to Japanese Language study, I realized that the time dedicated to writing classes mentioned in curriculum in not enough. In the limited school credits are also included writing lessons. I think that we must focus more on intermediate level writing lesson. In terms of student’s motivation and needs, the early intermediate level should contain courses focused on intermediate vocabulary and representations. So, it would be likely that the second half of the intermediate level will treat academic Japanese and business circles. There are parts not enough treated during the courses, so the student must study them by himself. The teaching materials must facilitate and guide the student in these situations. Moreover, Japanese native teachers should be responsible for activities like correcting specific mistakes made by Korean native speakers, Korean-Japanese translation practice, activities included in the Japanese intermediate level writing lessons. We must consider processes for creating compositions to support the intermediate level writing courses. For example: brainstorming to create and verify sentences, rough copy, peer response and feedback from the teacher, revisions and proofreading, presentation the created compositions, etc. I think that an effective method is to increase the student monitoring feature, so that the teacher can point out when an incorrect language use occurred. The students will make further practice while discussing between them the correct form. Moreover, we must introduce peer response to make compositions through cooperation between students, and at the same time having a feedback from the teacher. It is important to create an environment of cooperation by mobilizing the human resources provided by the classroom to create compositions. Through this kind of classroom activities, the essays quality level made by students after the courses rises. The feedback made by teachers regarding the sentence comprehending and essay composition will make possible a higher perspective.
韓国語と中国語を母語とする日本語学習者の聴解力 : 聴解力の差、聴解力と読解力との関連
小笹克行(Katsuyuki Ozasa) 한국일본문화학회 2002 日本文化學報 Vol.13 No.-
This study investigated listening ability of Korean native and Chinese native JSL learners by the statistical ways. As a result Korean native speaker was superior than Chinese native speaker in listening ability at certain level. And this study also investigated when both learners acquired Japanese language how they related listening ability to reading ability. Consequently both learners showed different change.
Sung‑Hyun Park,Ozkan Gokcekaya,Ryosuke Ozasa,Myung‑Hoon Oh,Young‑Won Kim,Hyoung Seop Kim,Takayoshi Nakano 대한금속·재료학회 2024 METALS AND MATERIALS International Vol.30 No.5
The microstructural evolution and crystallographic texture formation of β-solidifying Ti-44Al-6Nb-1.2Cr alloy were identifiedunder single- and multi-track exposures via laser powder bed fusion (L-PBF) for various process parameters. Undersingle-track exposure, the microstructure of the melt pool was divided into the band-like α2 phase in the melt pool boundaryand β phase in the melt pool center. Numerical and thermodynamic simulations revealed that the underlying mechanism ofphase separation was related to the variation in the cooling rate in the melt pool, whereas microsegregation induced a shiftin the solidification path. Meanwhile, the crystallographic texture of the α2 phase region was identical to that of the substrateowing to the epitaxial growth of the β phase and subsequent α phase nucleation. In contrast, the β phase exhibited a ± 45°inclined <100> alignment in the melt pool, which was tilted to align along the build direction toward the center of the meltpool corresponding to the simulated thermal gradient direction. Furthermore, the narrow hatch space condition maintainedthe crystallographic texture to the subsequent scan, forming a continuous band-like α2 phase with a strong selection. However,the crystallographic texture in a wide hatch space condition manifested a random distribution and constituted a finemixture of the β and α2 phases. For the first time, these results will offer an understanding of an anisotropic microstructurecontrol via the L-PBF process and ensure the tailoring of the mechanical properties in the β-solidifying γ-TiAl-based alloysby approaching hatch spacing control.