Impact of long-term high-intensity interval and moderate-intensity continuous training on subclinical inflammation in overweight/obese adults

José Gerosa-Neto,Barbara M.M. Antunes,Eduardo Z. Campos,Jhennyfer Rodrigues,Gustavo D. Ferrari,José C. Rosa Neto,Carlos R. Bueno Junior,Fábio S. Lira 한국운동재활학회 2016 JER Vol.12 No.6

Study of Stay Vanes Vortex-Induced Vibrations with different Trailing-Edge Profiles Using CFD

Neto, Alexandre D'Agostini,Saltara, Fabio Korean Society for Fluid machinery 2009 International journal of fluid machinery and syste Vol.2 No.4

The 2D flow around 13 similar stay-vane profiles with different trailing edge geometries is investigated to determinate the main characteristics of the excitation forces for each one of them and their respective dynamic behaviors when modeled as a free-oscillating system. The main goal is avoid problems with cracks of hydraulic turbines components. A stay vane profile with a history of cracks was selected as the basis for this work. The commercial finite-volume code $FLUENT^{(R)}$ was employed in the simulations of the stationary profiles and, then, modified to take into account the transversal motion of elastically mounted profiles with equivalent structural stiffness and damping. The k-$\omega$ SST turbulence model is employed in all simulations and a deforming mesh technique used for models with profile motion. The static-model simulations were carried out for each one of the 13 geometries using a constant far field flow velocity value in order to determine the lift force oscillating frequency and amplitude as a function of the geometry. The free-oscillating stay-vane simulations were run with a low mass-damping parameter ($m^*{\xi}=0.0072$) and a single mean flow velocity value (5m/s). The structural bending stiffness of the stay-vane is defined by the Reduced Velocity parameter (Vr). The dynamic analyses were divided into two sets. The first set of simulations was carried out only for one profile with $2{\leq}Vr{\leq}12$. The second set of simulations focused on determining the behavior of each one of the 13 profiles in resonance.

Using Practice Context Models to Knowledge Management in Proof-of-Concept Activities: A Contribution of Knowledge Networks and Percolation Theory

Neto, Antonio Jose Rodrigues,Borges, Maria Manuel,Roque, Licinio Korea Institute of Science and Technology Informat 2021 Journal of Information Science Theory and Practice Vol.9 No.1

This study introduces novel research using Practice Context Models supported by Knowledge Networks and Percolation Theory with the aim to contribute to knowledge management in Proof-of-Concept (PoC) activities. The authors envision this proposal as a potential instrument to identify network structures based on a percolation (propagation) threshold and to analyze the importance of nodes (e.g., practitioners, practices, competencies, movements, and scenarios) during the percolation of knowledge in PoC activities. After thirty months immersed in the natural PoC habitat, acting as observers and practitioners, and supported by an ethnographic exercise and a designer-research mindset, the authors identified the production of meaning in PoC activities occurring in a hermeneutic circle characterized by the presence of several knowledge networks; thus, discovering the 'natural knowledge' in PoC as a spectrum of cognitive development spread throughout its network, as each node could produce and disseminate certain knowledge that flows and influences other nodes. Therefore, this research presents the use of Practice Context Models 'connected' to Knowledge Networks and Percolation Theory as a potential and feasible proposal to be built using the attribution of values (weights) to the nodes (e.g., practitioners, practices, competencies, movements, scenarios, and also knowledge) in the context of PoC with the aim to allow the players (e.g., PoC practitioners) to have more flexibility in building alliances with other players (new nodes); that is, focusing on those nodes with higher value (focus on quality) in collaboration networks, i.e., alliances (connections) with the aim to contribute to knowledge management in the context of PoC.

Feed Restriction and Compensatory Growth in Guzerá Females

Neto, S. Gonzaga,Bezerra, L.R.,Medeiros, A.N.,Ferreira, M.A.,Filho, E.C. Pimenta,Candido, E.P.,Oliveira, R.L. Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.6

This study examined the effect of restricting feed intake and the subsequent compensatory growth in Guzera females. Eighteen animals with an initial age of 21 months and a mean weight of 268.17 kg were placed in three groups according to the alimentary regime: feed ad libitum; feed restricted to 20% dry matter; and feed restricted to 40% dry matter. In the restricted feed phase, the dry mater intake decreased as the restriction levels increased, influencing the reduction in intake of other nutrients. In the realimentation phase, the 40% restricted feed group ingested more dry matter (% BW) and crude protein ($weight^{0.75}$) than the group fed ad libitum (p<0.001). The serum nutrient concentrations were inversely proportional (p<0.001) to the restriction level, and there was no difference (p>0.001) in the realimentation phase. In the restricted feed phase, the final live weight decreased (p<0.05) as the restriction level increased. For the daily mean weight gain in the control group, there was no difference (p>0.05) compared to the animals with 20% feed restriction, but this was higher than in the group with 40% feed restriction. In the re-alimentation phase, the group with 40% feed restriction achieved higher weight gain rates, which was different from the control and 20% restriction groups. In both phases, the animals in the group with 40% feed restriction presented better feed conversion which was different (p<0.05) from the control group. In the feed restriction phase, it was observed that the intake of N, nitrogen excreted in feces and urine, nitrogen balance and nitrogen retention decreased (p<0.05) with the restriction level. None of the variables were influenced in the re-alimentation phase. These results show that feed restriction by 40% can be adopted as a nutritional management practice.

Pyrearinus termitilluminans larval click beetle luciferase: active site properties, structure and function relationships and comparison with other beetle luciferases

Neto, A.J.Silva,Scorsato, V.,Arnoldi, F.G.C.,Viviani, V.R. Korean Society of Photoscience 2009 Photochemical & photobiological sciences Vol.8 No.12

Several beetle luciferases have been cloned and sequenced. However, most studies on structure and function relationships and bioanalytical applications were done with firefly luciferases, which are pH sensitive. Several years ago we cloned Pyrearinus termitilluminans larval click beetle luciferase, which displays the most blue-shifted bioluminescence among beetle luciferases and is pH insensitive. This enzyme was expressed in E. coli, purified, and its properties investigated. This luciferase shows slower luminescence kinetics, $K_M$ values comparable to other beetle luciferases and high catalytic constant. Fluorescence studies with 8-anilino-1-naphtalene-sulfonic acid (1,8-ANS) and modeling studies suggest that the luciferin binding site of this luciferase is very hydrophobic, supporting the solvent and orientation polarizability effects as determining mechanisms for bioluminescence colors. Although pH insensitive in the range between pH 6-8, at pH 10 this luciferase displays a remarkable red-shift and broadening of the bioluminescence spectrum. Modeling studies suggest that the residue C312 may play an important role in bioluminescence color modulation. Compared to other beetle luciferases, Pyrearinus termitilluminans luciferase also displays higher thermostability and sustained luminescence in a bacterial cell environment, which makes this luciferase particularly suitable for in vivo cell analysis and bioimaging.

Sensory neurons and osteoblasts: close partners in a microfluidic platform

Neto, Estrela,Alves, Cecí,lia J.,Sousa, Daniela M.,Alencastre, Inê,s S.,Lourenç,o, Ana H.,Leitã,o, Luí,s,Ryu, Hyun R.,Jeon, Noo L.,Fernandes, Rui,Aguiar, Paulo,Almeida, Ra Oxford University Press 2014 Integrative biology Vol.6 No.6

<P>Innervation has proven to be critical in bone homeostasis/regeneration due to the effect of soluble factors, produced by nerve fibers, associated with changes in the activity of bone cells. Thus, in this study, we have established and characterized a coculture system comprising sensory neurons and osteoblasts to mimic the <I>in vivo</I> scenario where nerve fibers can be found in a bone microenvironment. Embryonic or adult primary dorsal root ganglion (DRG) and MC3T3-E1 osteoblastic cells were cocultured in compartmentalized microfluidic platforms and morphological and functional tests were performed. The time of adhesion and readout of axonal outgrowth were improved by the alignment of DRG with the axis of microgrooves, which showed to be a crucial step for the designed experiments. Cocultures of entire DRG from adult origin with osteoblasts were performed, showing extended DRG projections towards the axonal compartment, reaching osteoblastic cells. Immunocytochemistry showed that the neurites present within the osteoblastic compartment were immunoreactive to synapsin and calcitonin gene-related peptide suggesting the presence of specialized structures involved in this crosstalk. This evidence was further confirmed by electron microscopy where varicosities were detected as well as electron dense structures in neurite membranes. Aiming to mimic the properties of tissue extracellular matrices, MC3T3-E1 cells were seeded in the axonal side upon laminin, collagen or within 3D functionalized alginate matrices and axonal outgrowth was clearly observed. In order to analyze and quantify data with reproducible image analysis, a semi-automated algorithm was also developed. The collagen and laminin substrates displayed a higher amount of axons reaching the axonal side. Overall, the established method revealed to be a suitable tool to study the interaction between the peripheral nervous system and bone cells in different contexts mimicking the <I>in vivo</I> scenario.</P>

Hyperbaric oxygen therapy for the treatment of a crush injury of the hand: a case report

Neto Pedro Henry,Ribeiro Zamara Brandão,Pinho Adriano Bastos,Almeida Carlos Henrique Rodrigues de,Maranhão Carlos Alberto de Albuquerque,Goncalves Joaquim da Cunha Campos 대한외상학회 2022 大韓外傷學會誌 Vol.35 No.3

We describe a case of hyperbaric oxygen therapy (HBOt) as an adjunct to treatment of a crush injury to the hand. A 34-year-old male paramedic was involved in a motor vehicle accident and admitted for diagnosis and surgical treatment. He sustained a crush injury to his right hand and presented with significant muscle damage, including multiple fractures and dislocations, an avulsion injury of the flexor tendons, and amputation of the distal phalanx of the little finger. He underwent reconstructive surgery and received HBOt over the following days. In the following 2 months, he lost the distal and middle phalanges of the little finger and recovered hand function. Posttraumatic compartment syndrome responds well to HBOt, which reduces edema and contributes to angiogenesis, as well as promoting the cascade of healing events. High-energy trauma causes massive cell destruction, and the blood supply is usually not sufficient to meet the oxygen demands of viable tissues. Hyperbaric oxygenation by diffusion through interstitial and cellular fluids increases tissue oxygenation to levels sufficient for the host’s responses to injury to work and helps control the delayed inflammatory reaction. HBOt used as an adjunct to surgical treatment resulted in early healing and rehabilitation, accelerating functional recovery. The results suggest that adjunctive HBOt can be beneficial for the treatment of crush injuries of the hand, resulting in better functional outcomes and helping to avoid unnecessary amputations.

Developing Digital Games through Software Reuse

Neto, Beatriz,Fernandes, Lucia,Werner, Claudia,De Souza, Jano Moreira Korea Information Processing Society 2010 Journal of information processing systems Vol.6 No.2

Gaming is an old humans' habit. Games help in logical development and encourage learning of theoretical and practical concepts. Besides they offer entertainment and challenge. The advent of the personal computer changed this tradition. Every year new challenges arise in a digital format, which lead the young and adults to spend hours in front of a computer or TV screen in an attempt to overcome hurdles and reach an objective. Quality, sophistication, and constant innovation are attained through complex computer software that almost has an obligation to improve as each new title is released, due to this game development becomes a challenge. Considering that a game title is software and thus faces the same restrictions of business applications, this article intends to analyze, under the optics of reuse, if game development resorts to reuse, and where and how this happens.

Sentinel Lymph Node Navigation Surgery for Early Gastric Cancer: Is It a Safe Procedure in Countries with Non-Endemic Gastric Cancer Levels? A Preliminary Experience

Neto, Guilherme Pinto Bravo,Santos, Elizabeth Gomes Dos,Victer, Felipe Carvalho,Neves, Marcelo Soares,Pinto, Marcia Ferreira,Carvalho, Carlos Eduardo De Souza The Korean Gastric Cancer Association 2016 Journal of gastric cancer Vol.16 No.1

Purpose: Early diagnosis of gastric cancer is still the exception in Western countries. In the East, as in Japan and Korea, this disease is an endemic disorder. More conservative surgical procedures are frequently performed in early gastric cancer cases in these countries where sentinel lymph node navigation surgery is becoming a safe option for some patients. This study aims to evaluate preliminary outcomes of patients with early gastric cancer who underwent sentinel node navigation surgeries in Brazil, a country with non-endemic gastric cancer levels. Materials and Methods: From September 2008 to March 2014, 14 out of 205 gastric cancer patients underwent sentinel lymph node navigation surgeries, which were performed using intraoperative, endoscopic, and peritumoral injection of patent blue dye. Results: Antrectomies with Billroth I gastroduodenostomies were performed in seven patients with distal tumors. The other seven patients underwent wedge resections. Sentinel basin resections were performed in four patients, and lymphadenectomies were extended to stations 7, 8, and 9 in the other 10. Two patients received false-negative results from sentinel node biopsies, and one of those patients had micrometastasis. There was one postoperative death from liver failure in a cirrhotic patient. Another cirrhotic patient died after two years without recurrence of gastric cancer, also from liver failure. All other patients were followed-up for 13 to 79 months with no evidence of recurrence. Conclusions: Sentinel lymph node navigation surgery appears to be a safe procedure in a country with non-endemic levels of gastric cancer.

An Image-Based High-Content Screening Assay for Compounds Targeting Intracellular <i>Leishmania donovani</i> Amastigotes in Human Macrophages

Siqueira-Neto, Jair L.,Moon, Seunghyun,Jang, Jiyeon,Yang, Gyongseon,Lee, Changbok,Moon, Hong Kee,Chatelain, Eric,Genovesio, Auguste,Cechetto, Jonathan,Freitas-Junior, Lucio H. Public Library of Science 2012 PLoS neglected tropical diseases Vol.6 No.6

<▼1><P>Leishmaniasis is a tropical disease threatening 350 million people from endemic regions. The available drugs for treatment are inadequate, with limitations such as serious side effects, parasite resistance or high cost. Driven by this need for new drugs, we developed a high-content, high-throughput image-based screening assay targeting the intracellular amastigote stage of different species of <I>Leishmania</I> in infected human macrophages. The <I>in vitro</I> infection protocol was adapted to a 384-well-plate format, enabling acquisition of a large amount of readouts by automated confocal microscopy. The reading method was based on DNA staining and required the development of a customized algorithm to analyze the images, which enabled the use of non-modified parasites. The automated analysis generated parameters used to quantify compound activity, including infection ratio as well as the number of intracellular amastigote parasites and yielded cytotoxicity information based on the number of host cells. Comparison of this assay with one that used the promastigote form to screen 26,500 compounds showed that 50% of the hits selected against the intracellular amastigote were not selected in the promastigote screening. These data corroborate the idea that the intracellular amastigote form of the parasite is the most appropriate to be used in primary screening assay for <I>Leishmania</I>.</P></▼1><▼2><P><B>Author Summary</B></P><P>Leishmaniasis, one of the most neglected tropical diseases, affects over 2 million people each year. Visceral leishmaniasis (VL), also known as Kala-azar, is caused by the protozoan parasites <I>Leishmania donovani</I> and <I>Leishmania infantum</I> and is fatal if left untreated. Because existing treatments are often ineffective due to parasite resistance and/or toxicity new drugs are urgently needed. Leishmaniasis is transmitted to humans by the bite of an infected sandfly. In the insect vector, parasites exist as flagellated forms—promastigotes, which infect macrophage cells of the human host, where they differentiate to round forms known as amastigotes. Amastigotes and promastigotes are substantially different from a molecular perspective. Drug discovery for leishmaniasis has traditionally been complicated by the unavailability of validated drug targets and of relevant drug assays. Whole cell-based assays have been widely used, as they bypass the need for a validated target. However, they use the insect form of the parasite; indeed, the human form, the intracellular amastigote, is difficult to obtain in the laboratory in quantities compatible with drug screening. We describe here the technical advances that made it possible to adapt the intracellular amastigote form of <I>L. donovani</I> to a drug assay compatible with high-throughput screening.</P></▼2>