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        Fabrication of a sensitive electrochemical sensor based on Ag nanoparticles and alizarin yellow polymer: Application to the detection of an environmental pollutant thiourea

        Iraj Jodan,Kitirote Wantala,Nader Amini,Behzad Shahmoradi,Mona Ghaslani,이승목,Jixiang Yang,Shivaraju Harikaranahalli Puttaiah 한국화학공학회 2020 Korean Journal of Chemical Engineering Vol.37 No.9

        The harmful and destructive effects of excessive consumption of thiourea in various industries and agriculture have caused health and environmental concerns. Hence, attention has been paid to the need for analysis and detection thiourea at very low concentrations. The aim of this study was to determine the efficiency of electrochemical sensor based on glass carbon electrode modified with Ag nanoparticles and alizarin yellow to detect thiourea as a pollutant. Glass carbon electrode was first modified by alizarin yellow polymer and Ag nanoparticles through electropolymerization and chronoamperometry methods, respectively. The characteristics of the modified electrode were determined through FESEM and EDAX analysis and thiourea concentration was measured through cyclic voltammetry (CV) and differential pulse voltammetry (DPV) methods. The results showed that the linear range, limit of detection, and sensitivity were 10 to 940 M, 3.3 M and 0.181 A/M, respectively. The stability and reproducibility of the sensor response as well as the interference effect of some anion species were investigated on the performance of the sensor in determining thiourea. The results indicate the optimum stability and reproducibility and no interference effect of the studied species on efficiency of the sensor in determining thiourea.

      • Disorders of Liver : In Vivo Protoscolicidal Effect Of Hypertonic Glucose On Protoscolices Of Hydatid Cyst

        ( Syed Vahid Hosseini ),( Seyed Mahmoud Adjjadi ),( Davood Mehrabani ),( Kurosh Ghanbarzaden ),( Zahra Barzin ),( Nader Tanideh ),( Masoud Amini ) 대한소화기학회 2007 SIDDS Vol.9 No.-

        Background/Aims: The treatment of choice for Hydatidosis is surgery, but before evacuation of a cyst, protoscolicidal agents are needed to be injected to prevent a secondary cyst formation and the spreading of the disease. This study was performed to determine the protoseolicidal effect of various concentrations of hypertonic glucose in an animal model infected to hydatid cyst. Methods: Sheep liver hydatid cyst surface were sterilized by heat and the cyst contents were evacuated completely and transferred into falcon tubes, where protoscolices were precipitated and separated. The viability of protoseolices was determined by using eosin stain method. A total of 1500 protoscolices were exposed to 0.5% cetrimide, 0.5% silver nitrate, 0.9% and 20% sodium chloride and 10%, 15%, 25%, and 50% concentrations of glucose with exposure times of one, 2 and 5 minutes and were injected into the peritoneal cavity of 5 BALB/c mice for each agent. The animals were kept for 9 months and then were autopsied. Blood sample for countercurrent immunoelectrophoresis (CCIEP) was provided and the animals were examined for presence of any cyst in peritoneal cavity and recorded. Results: A total of 40 BALB/C mice were used in all exposure times while 24, 21 and 32 mice survived when exposed to different protoscilicidal agents after 1, 2 and 5 minutes respectively. None of the mice were infected to cysts after exposure to 50% hypertonic glucose solution. Identical to 50% glucose solution no infection was noticed for 0.5% cetrimide and 20% sodium chloride solutions too. The CCIEP results of animals indicating to cyst formation confirmed the presence of antibody in the circulation. Conclusions: Similarity of 50% glucose solution and cetrimide and 20% sodium chloride in complete killing of protoscolices present it as potent protoscolicidal agent and the side effects of cetrinide and hypertonic sodium chloride indicate to further studies on probable side effects.

      • Disorders of Liver : The Role Of Germinative Layer In Inducing Hydatid Cyst

        ( Seyed Vahid Hosseini ),( Soheal Mansoorian ),( Seyed Mahmood Sadjjadi ),( Davood Mehrabani ),( Zahra Barzin ),( Nader Tanideh ),( Afshin Amini ),( All Rafati ),( Masoud Amini ) 대한소화기학회 2007 SIDDS Vol.9 No.-

        Background/Aims: In spite of use of protoseololeidal agents during surgery, a notable rate of recurrence of disease in post-operation patients is still observed. The question exists is that alive scolices would cause any recurrence or is due to the presence of the germinative layer. The aim of this study was to evaluate the effect of scolicidal agents on this layer as well as its ability of the layer to produce any hydatid disease in an in vivo condition. Methods: Germinative layer of hydatid cyst was separated under sterile condition, was divided into 0.5 cm parts, exposed to various protoseolicidal agents for 2 minutes, and implanted in peritoneal cavity of 62 BALB-C mice. After 9 months, the peritoneum was evaluated macroscopically and microscopically for the presence of any hydatid disease. Results: After 9 months, no hydatid disease was visible in the peritoneum of all the sixty two mice. Conclusions: In this study, the germinative layer could not cause any hydatid disease but positive results of Tarakanovet al. with cultures of protoseolices and the germinative layer cells in the peritoneal cavity mice indicate to a need of another study of implantation of culture cells of the germinated layer lacking protoscolices in a larger animal study to clarify the differences.

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