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RISS 인기검색어
- 검색키워드
- 저자 : ( Seyed Mahmoud Adjjadi ) (검색결과 1 건)
- 무료
- 기관 내 무료
- 유료
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( Syed Vahid Hosseini ),( Seyed Mahmoud Adjjadi ),( Davood Mehrabani ),( Kurosh Ghanbarzaden ),( Zahra Barzin ),( Nader Tanideh ),( Masoud Amini ) 대한소화기학회 2007 SIDDS Vol.9 No.-
Background/Aims: The treatment of choice for Hydatidosis is surgery, but before evacuation of a cyst, protoscolicidal agents are needed to be injected to prevent a secondary cyst formation and the spreading of the disease. This study was performed to determine the protoseolicidal effect of various concentrations of hypertonic glucose in an animal model infected to hydatid cyst. Methods: Sheep liver hydatid cyst surface were sterilized by heat and the cyst contents were evacuated completely and transferred into falcon tubes, where protoscolices were precipitated and separated. The viability of protoseolices was determined by using eosin stain method. A total of 1500 protoscolices were exposed to 0.5% cetrimide, 0.5% silver nitrate, 0.9% and 20% sodium chloride and 10%, 15%, 25%, and 50% concentrations of glucose with exposure times of one, 2 and 5 minutes and were injected into the peritoneal cavity of 5 BALB/c mice for each agent. The animals were kept for 9 months and then were autopsied. Blood sample for countercurrent immunoelectrophoresis (CCIEP) was provided and the animals were examined for presence of any cyst in peritoneal cavity and recorded. Results: A total of 40 BALB/C mice were used in all exposure times while 24, 21 and 32 mice survived when exposed to different protoscilicidal agents after 1, 2 and 5 minutes respectively. None of the mice were infected to cysts after exposure to 50% hypertonic glucose solution. Identical to 50% glucose solution no infection was noticed for 0.5% cetrimide and 20% sodium chloride solutions too. The CCIEP results of animals indicating to cyst formation confirmed the presence of antibody in the circulation. Conclusions: Similarity of 50% glucose solution and cetrimide and 20% sodium chloride in complete killing of protoscolices present it as potent protoscolicidal agent and the side effects of cetrinide and hypertonic sodium chloride indicate to further studies on probable side effects.
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