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Yang, Jae-Wook,Jung, Won-Kyo,Lee, Chang-Min,Yea, Sung Su,Choi, Yung Hyun,Kim, Gi-Young,Lee, Dae-Sung,Na, Giyoun,Park, Sae-Gwang,Seo, Su-Kil,Choi, Jung Sik,Lee, Young-Min,Park, Won Sun,Choi, Il-Whan Informa Healthcare USA, Inc. 2014 IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY Vol.36 No.5
<P><I>Context</I>: Expression of various inflammatory mediators in corneal fibroblasts contributes to corneal inflammation.</P><P><I>Objective</I>: The purpose of this study was to assess the possible effects of caffeic acid phenethyl ester (CAPE) on the expression of inflammatory mediators during an inflammatory response in human corneal fibroblasts.</P><P><I>Materials and methods</I>: The levels of interleukin (IL)-6, monocyte chemotactic protein (MCP)-1, and intercellular adhesion molecule-1 (ICAM-1) from IL-1β-exposed human corneal fibroblasts were measured with enzyme-linked immunosorbent assays (ELISA). The regulatory mechanisms of CAPE on cellular signaling pathways were examined using Western blot and electrophoretic mobility shift assays. A functional validation was carried out by evaluating the inhibitory effects of CAPE on neutrophil and monocyte migration <I>in vitro</I>.</P><P><I>Results</I>: CAPE inhibited the expression of IL-6, MCP-1 and ICAM-1 induced by the pro-inflammatory cytokine IL-1β in corneal fibroblasts. The activation of AKT and NF-κB by IL-1β was markedly inhibited by CAPE, whereas the activity of mitogen-activated protein kinases (MAPKs) was not affected. CAPE significantly suppressed the IL-1β-induced migration of differentiated (d)HL-60 and THP-1 cells.</P><P><I>Discussion</I>: These anti-inflammatory effects of CAPE may be expected to inhibit the infiltration of leukocytes into the corneal stroma <I>in vivo</I>.</P>
Association of the G134A and G184C Polymorphisms in the CYP1A1 Gene with Lung Cancer Incidence
Ryu, Doug-Young,Huang, Ming-Ai,Park, Chang-Bo,Chang, Soo-Im,Im, Ruth,Choi, Seong-Jin,Kim, Na-Young,Park, In-Won,Choi, Byoung-Whui,Kim, Jae-Yeol,Shin, Jong-Wook,Choi, Jae-Chul,Choi, Byung-Sun,Park, Jun Korean Society of ToxicologyKorea Environmental Mu 2008 Toxicological Research Vol.25 No.3
The G184C and G134A single nucleotide polymorphisms(SNPs) of the CYP1A1 gene result in Ala62Pro and Gly45Asp substitutions, respectively. Here, we tested whether these SNPs are associated with an alteration in lung cancer incidence. We examined 80 Korean subjects with lung cancer and 240 age- and sex-matched controls. For each subject, the CYP1A1 gene was PCR amplified and sequenced. We observed that the odds ratio(OR) for lung cancer was 3.37 higher in subjects with the G184C polymorphism than in controls(95% confidence interval(CI), $0.89{\sim}12.73$, P=0.07). In contrast, the OR for lung cancer was 1.23 in subjects with the G134A polymorphism compared to controls(95% CI, $0.68{\sim}2.20$, P=0.49). The G184C polymorphism exacerbated the effects of smoking on lung cancer development. Gene-smoking interaction analyses revealed that past or present smokers with the G184C polymorphism had a higher incidence of lung cancer(OR, 24.72; 95% CI, $4.48{\sim}136.31$; P<0.01) than control smokers(OR, 6.65; 95% CI, $2.72{\sim}16.28$; P<0.01). However, there was only a slight difference in the ORs for lung cancer between control smokers and smokers with the G134A polymorphism. These findings suggest that the G184C polymorphism, but not the G134A polymorphism, is associated with an increased risk of lung cancer.
Association of the G134A and G184C Polymorphisms in the CYP1A1 Gene with Lung Cancer Incidence
Doug-Young Ryu,Mingai Huang,Changbo Park,Soo Im Chang,Ruth Im,Seong-Jin Choi,Na-Young Kim,In Won Park,Byoung Whui Choi,Jae Yeol Kim,Jong Wook Shin,Jae Chul Choi,Byung-Sun Choi,Jung-Duck Park 한국독성학회 2008 Toxicological Research Vol.24 No.2
The G184C and G134A single nucleotide polymorphisms (SNPs) of the CYP1A1 gene result in Ala62Pro and Gly45Asp substitutions, respectively. Here, we tested whether these SNPs are associated with an alteration in lung cancer incidence. We examined 80 Korean subjects with lung cancer and 240 age- and sex-matched controls. For each subject, the CYP1A1 gene was PCR amplified and sequenced. We observed that the odds ratio (OR) for lung cancer was 3.37 higher in subjects with the G184C polymorphism than in controls (95% confidence interval (CI), 0.89~12.73, P = 0.07). In contrast, the OR for lung cancer was 1.23 in subjects with the G134A polymorphism compared to controls (95% CI, 0.68~2.20, P = 0.49). The G184C polymorphism exacerbated the effects of smoking on lung cancer development. Gene-smoking interaction analyses revealed that past or present smokers with the G184C polymorphism had a higher incidence of lung cancer (OR, 24.72; 95% CI, 4.48~136.31; P < 0.01) than control smokers (OR, 6.65; 95% CI, 2.72~16.28; P < 0.01). However, there was only a slight difference in the ORs for lung cancer between control smokers and smokers with the G134A polymorphism. These findings suggest that the G184C polymorphism, but not the G134A polymorphism, is associated with an increased risk of lung cancer.
Transient Blindness in a Patient with Severe Metformin-Associated Lactic Acidosis(MALA)
Jae Wan Jeon,Wonjung Choi,Hae Ri Kim,Young Rok Ham,Dae Eun Choi,Ki Ryang Na,Kang Wook Lee,Soo Ya Bae,Seong Hoon Kim 전해질고혈압연구회 2019 Electrolytes & Blood Pressure Vol.17 No.1
A 68-year-old man presented at the emergency room with sudden blindness. The day before, he had eaten sashimi and eel and drank alcohol for dinner. He experienced nausea, vomiting, and dizziness afterward. His medical his- tory included hypertension and diabetes, and the latter was treated with met- formin. Initial laboratory tests revealed severe metabolic acidosis(lactic aci- dosis). Massive hydration and intravenous sodium bicarbonate replacement therapies were initiated, but severe metabolic acidosis(lactic acidosis) did not resolve, in turn, leading to hemodialysis, which decreased metabolic aci- dosis. The patient’s blindness improved, and his vision gradually recovered. As it is not easy to distinguish between blindness related to metformin-asso- ciated lactic acidosis(MALA) and blindness related to other causes, rapid correction of metabolic acidosis through hemodialysis might be helpful in differentiating this from of blindness from blindness related to other causes.
Na Sang-Kuwon,Kong Byeong-Gi,Choi Changyong,Jang Mi-Kyeong,Nah Jae-Woon,Kim Jung-Gyu,Jo Byung-Wook The Polymer Society of Korea 2005 Macromolecular Research Vol.13 No.2
Melt blending of Bisphenol A polycarbonate (PC) and poly(trimethylene terephthalate) (PTT) was carried out over the entire composition range. The mixing time was varied up to 90 min. The resulting samples were analyzed by FT-IR, DSC, XRD, DMTA, $^{1}H NMR$, and SEM. The process of transesterification between the two polymers and their resulting compatibilization were observed. The behaviors of the PTT-rich and PC-rich blends were different and an equilibrium was found to exist. Peculiar behavior, which was different from that of the PTT-rich and PC-rich blends, was exhibited by the 50/50 (PTT/PC) blend.
A Novel Feeder-Free Culture System for Expansion of Mouse Spermatogonial Stem Cells
Choi, Na Young,Park, Yo Seph,Ryu, Jae-Sung,Lee, Hye Jeong,Arauzo-Bravo, Marcos J.,Ko, Kisung,Han, Dong Wook,Scholer, Hans R.,Ko, Kinarm Korean Society for Molecular and Cellular Biology 2014 Molecules and cells Vol.37 No.6
Spermatogonial stem cells (SSCs, also called germline stem cells) are self-renewing unipotent stem cells that produce differentiating germ cells in the testis. SSCs can be isolated from the testis and cultured in vitro for long-term periods in the presence of feeder cells (often mouse embryonic fibroblasts). However, the maintenance of SSC feeder culture systems is tedious because preparation of feeder cells is needed at each subculture. In this study, we developed a Matrigel-based feeder-free culture system for long-term propagation of SSCs. Although several in vitro SSC culture systems without feeder cells have been previously described, our Matrigel-based feeder-free culture system is time- and cost-effective, and preserves self-renewability of SSCs. In addition, the growth rate of SSCs cultured using our newly developed system is equivalent to that in feeder cultures. We confirmed that the feeder-free cultured SSCs expressed germ cell markers both at the mRNA and protein levels. Furthermore, the functionality of feeder-free cultured SSCs was confirmed by their transplantation into germ cell-depleted mice. These results suggest that our newly developed feeder-free culture system provides a simple approach to maintaining SSCs in vitro and studying the basic biology of SSCs, including determination of their fate.
( Jae Woong Jeon ),( Hee Jung Yoon ),( Joo Seok Kim ),( Il Hwan Ryu ),( Ji Wook Choi ),( Min Gyu Kim ),( Young Min Na ),( Hyeon Jeong Yun ) 대한결핵 및 호흡기학회 2014 Tuberculosis and Respiratory Diseases Vol.76 No.2
Patients with acquired immunodeficiency syndrome (AIDS) are at higher risks of bacterial pneumonia than the general population, and the pathogen is the most commonly involved Streptococcus pneumoniae. We hereby report a case of pneumococcal pneumonia associated with leptomeningitis, osteomyelitis and epidural abscess in a patient with AIDS. He is being successfully treated with ampicillin/sulbactam and clindamycin. And because the pneumococcal infection is usually associated with morbidity and mortality rates in the setting of AIDS, we should consider for pneumococcal vaccinations among the AIDS populations.
Nicotine induces the expression of early growth response-1 in human skin dermal fibroblasts
Choi, Jae Eun,Kim, Ji Na,Jeong, Sang Hoon,Son, Sang Wook Blackwell Publishing Ltd 2010 International journal of dermatology Vol.49 No.2
<P>Abstract</P><P>Background </P><P>Egr-1 is known to play an important role in cell growth, differentiation, and cell survival. Human skin dermal fibroblasts (HSDF) may be an important target for adverse effects of tobacco components. However, nicotinic effect for early growth response-1 (Egr-1) expression is unknown in HSDF.</P><P>Methods </P><P>Cytotoxicity of nicotine was assessed by cell viability test in HSDF. The expression of Egr-1 protein and mRNA after nicotine treatment was evaluated by Western blotting and real-time reverse transcription–polymerase chain reaction. We also measured the promoter activity of Egr-1 in HSDF after nicotine exposure.</P><P>Results </P><P>Early growth response-1 protein and mRNA levels were increased in dermal fibroblasts exposed to nicotine. Nicotine treatment stimulated the promoter activity of Egr-1 in cultured human fibroblasts.</P><P>Conclusion </P><P>In this study, we demonstrate that Egr-1 expression is markedly induced in HSDF after exposure to nicotine.</P>
Reduction of COD and Color of Acid and Reactive Dyestuff Wastewater Using Ozone
Choi, Jae-Wook,Song, Hyung Keun,Lee, Whun,Koo, Kee-Kahb,Han, Choon,Na, Byung-Ki 한국화학공학회 2004 Korean Journal of Chemical Engineering Vol.21 No.2
Wastewaters obtained from bromamine acid dye and reactive dye manufacturing were treated by ozone bubbling in a cylindrical batch reactor for the purpose of reducing COD to below 150mg/L, which is the environmental discharge standard of the Republic of Korea. Remarkable COD reduction and decolorization were observed at pH over 11. High inlet gas flowrate and high concentration of ozone gave better results. Little precipitation was observed under the conditions of remarkable COD reduction. At pH of 11, 15 LPM of inlet gas flowrate and 89.3g/Nm^(3) of ozone flowrate, the COD of bromamine acid dyestuff wastewater was reduced to 95 mg/L after 90 minutes, and the COD of reactive dyestuff wastewater was reduced to 120 mg/L after 120 minutes. Decolorization was completed after 30 minutes of reaction.