Dynamic measurements of hydrate based gas separation in cooled silica gel

Mingjun Yang,Yongchen Song,Lanlan Jiang,Xiaojing Wang,Weiguo Liu,Yuechao Zhao,Yu Liu,Shanrong Wang 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.1

Hydrate based gas separation is a promising method for carbon dioxide capture. The purpose of thisstudy is to analyze hydrates formation and dissociation characters when gas mixture flows throughcooled silica gel. The additives mixture (THF/SDS) was used to saturate the silica gel partly, and gasmixture (CO2/H2) was injected into it to form hydrates. Magnetic resonance imaging (MRI) images wereobtained using fast spin echo multi-slice pulse sequence. Hydrates saturations were calculatedquantitatively using MRI data. The experimental results showed that the optimal initial solutionsaturation was 34.2% in this investigation. The gas component was analyzed to assess the separationefficiency. For hydrates dissociation processes at 1 atmospheric pressure, CO2 concentrations increasedobviously. Half of the six cycles showed that more than 85.00 mol% CO2 contained in the capture gas, andthe lowest CO2 concentration was 64.83 mol%. Hydrate blockages appeared frequently, which restrictedthe contact of gas and solution and caused the incomplete transformations of residual solution tohydrates. It was a key restricted factor for hydrate based CO2 capture.

Improving Granulosa Cell Function in Premature Ovarian Failure with Umbilical Cord Mesenchymal Stromal Cell Exosome-Derived hsa_circ_0002021

Yang Ge,Zhang Bo,Xu Mei,Wu MingJun,Lin Jie,Luo ZiYu,Chen YueHua,Hu Qin,Huang GuoPing,Hu HaiYan 한국조직공학과 재생의학회 2024 조직공학과 재생의학 Vol.21 No.6

Background: The therapeutic potential of exosomes from human umbilical cord mesenchymal stem cells (HUMSCs-Exo) for delivering specific circular RNAs (circRNAs) in treating premature ovarian failure (POF) is not well understood. This study aimed to explore the efficacy of HUMSCs-Exo in delivering hsa_circ_0002021 for POF treatment, focusing on its effects on granulosa cell (GC) senescence and ovarian function. Methods: Bioinformatic analysis was conducted on circRNA profiles using the GSE97193 dataset from GEO, targeting granulosa cells from varied age groups. To simulate granulosa cell senescence, KGN cells were treated with cyclophosphamide (CTX). HUMSCs were transfected with pcDNA 3.1 vectors to overexpress hsa_circ_0002021, and the HUMSCs-Exo secreted were isolated. These exosomes were characterized by transmission electron microscopy (TEM) and Western blotting to confirm exosomal markers CD9 and CD63. Co-culture of these exosomes with CTX-treated KGN cells was performed to assess β-galactosidase activity, oxidative stress markers, ROS levels, and apoptosis via flow cytometry. Interaction between hsa_circ_0002021, microRNA-125a-5p (miR-125a-5p), and cyclin-dependent kinase 6 (CDK6) was investigated using dual-luciferase assays and RNA immunoprecipitation (RIP). A POF mouse model was induced with CTX, treated with HUMSCs-Exo, and analyzed histologically and via immunofluorescence staining. Gene expression was quantified using RT-qPCR and Western blot. Results: hsa_circ_0002021 was under expressed in both in vivo and in vitro POF models and was effectively delivered by HUMSCs-Exo to KGN cells, showing a capability to reduce GC senescence. Overexpression of hsa_circ_0002021 in HUMSCs-Exo significantly enhanced these anti-senescence effects. This circRNA acts as a competitive adsorbent of miR-125a-5p, regulating CDK6 expression, which is crucial in modulating cell cycle and apoptosis. Enhanced expression of hsa_circ_0002021 in HUMSCs-Exo ameliorated GC senescence in vitro and improved ovarian function in POF models by modulating oxidative stress and cellular senescence markers. Conclusion: This study confirms that hsa_circ_0002021, when delivered through HUMSCs-Exo, can significantly mitigate GC senescence and restore ovarian function in POF models. These findings provide new insights into the molecular mechanisms of POF and highlight the therapeutic potential of circRNA-enriched exosomes in treating ovarian aging and dysfunction. Background: The therapeutic potential of exosomes from human umbilical cord mesenchymal stem cells (HUMSCs-Exo) for delivering specific circular RNAs (circRNAs) in treating premature ovarian failure (POF) is not well understood. This study aimed to explore the efficacy of HUMSCs-Exo in delivering hsa_circ_0002021 for POF treatment, focusing on its effects on granulosa cell (GC) senescence and ovarian function. Methods: Bioinformatic analysis was conducted on circRNA profiles using the GSE97193 dataset from GEO, targeting granulosa cells from varied age groups. To simulate granulosa cell senescence, KGN cells were treated with cyclophosphamide (CTX). HUMSCs were transfected with pcDNA 3.1 vectors to overexpress hsa_circ_0002021, and the HUMSCs-Exo secreted were isolated. These exosomes were characterized by transmission electron microscopy (TEM) and Western blotting to confirm exosomal markers CD9 and CD63. Co-culture of these exosomes with CTX-treated KGN cells was performed to assess β-galactosidase activity, oxidative stress markers, ROS levels, and apoptosis via flow cytometry. Interaction between hsa_circ_0002021, microRNA-125a-5p (miR-125a-5p), and cyclin-dependent kinase 6 (CDK6) was investigated using dual-luciferase assays and RNA immunoprecipitation (RIP). A POF mouse model was induced with CTX, treated with HUMSCs-Exo, and analyzed histologically and via immunofluorescence staining. Gene expression was quantified using RT-qPCR and Western blot. Results: hsa_circ_0002021 was under expressed in both in vivo and in vitro POF models and was effectively delivered by HUMSCs-Exo to KGN cells, showing a capability to reduce GC senescence. Overexpression of hsa_circ_0002021 in HUMSCs-Exo significantly enhanced these anti-senescence effects. This circRNA acts as a competitive adsorbent of miR-125a-5p, regulating CDK6 expression, which is crucial in modulating cell cycle and apoptosis. Enhanced expression of hsa_circ_0002021 in HUMSCs-Exo ameliorated GC senescence in vitro and improved ovarian function in POF models by modulating oxidative stress and cellular senescence markers. Conclusion: This study confirms that hsa_circ_0002021, when delivered through HUMSCs-Exo, can significantly mitigate GC senescence and restore ovarian function in POF models. These findings provide new insights into the molecular mechanisms of POF and highlight the therapeutic potential of circRNA-enriched exosomes in treating ovarian aging and dysfunction.

High photocatalytic degradation of tetracycline under visible light with Ag/AgCl/activated carbon composite plasmonic photocatalyst

Huiqin Wang,Xiaoqing Yang,Jinzhao Zi,Mingjun Zhou,Zhefei Ye,Jinze Li,Qingfeng Guan,Peng Lv,Pengwei Huo,Yongsheng Yan 한국공업화학회 2016 Journal of Industrial and Engineering Chemistry Vol.35 No.-

Ag/AgCl/activated carbon (AC) plasmonic photocatalysts were prepared by precipitation and photoreduc-tion method. The as-prepared plasmonic photocatalysts were characterized by XRD, SEM, BET, EDS, UV–visDRS, XPS and PL. The results show the composite photocatalysts are successfully obtained and obviouslyexhibit surface plasmon resonance and effectively enhance the separation of h+/e . The photocatalyticdegradation rate of tetracycline (TC) could reach 97.3% in 60 min under visible light irradiation and cycledexperiments show stably photocatalytic activities. The active species analysis displays holes andsuperoxide radicals are the main reactive species. Photocatalytic kinetic indicates that the degradationprocesses obey pseudo first order kinetic equation.

Energy-Efficient Traffic Grooming in Bandwidth Constrained IP over WDM Networks

( Bin Chen ),( Zijian Yang ),( Rongping Lin ),( Mingjun Dai ),( Xiaohui Lin ),( Gongchao Su ),( Hui Wang ) 한국인터넷정보학회 2018 KSII Transactions on Internet and Information Syst Vol.12 No.6

Minimizing power consumption in bandwidth limited optical traffic grooming networks is presented as a two-objective optimization problem. Since the main objective is to route a connection, the network throughput is maximized first, and then the minimum power consumption solution is found for this maximized throughput. Both transparent IP over WDM (Tp-IPoWDM) and translucent IP over WDM (Tl-IPoWDM) network may be applied to examine such bi-objective algorithms. Simulations show that the bi-objective algorithms are more energy-efficient than the single objective algorithms where only the throughput is optimized. For a Tp-IPoWDM network, both link based ILP (LB-ILP) and path based ILP (PB-ILP) methods are formulated and solved. Simulation results show that PB-ILP can save more power than LB-ILP because PB-ILP has more path selections when lightpath lengths are limited. For a Tl-IPoWDM network, only PB-ILP is formulated and we show that the Tl-IPoWDM network consumes less energy than the Tp-IPoWDM network, especially under a sparse network topology. For both kinds of networks, it is shown that network energy efficiency can be improved by over-provisioning wavelengths, which gives the network more path choices.

Investigation on effect of surface properties on droplet impact cooling of cladding surfaces

Wang, Zefeng,Qu, Wenhai,Xiong, Jinbiao,Zhong, Mingjun,Yang, Yanhua Korean Nuclear Society 2020 Nuclear Engineering and Technology Vol.52 No.3

During transients or accidents, the reactor core is uncovered, and droplets entrained above the quench front collides with the uncovered fuel rod surface. Droplet impact cooling can reduce the peak cladding temperature. Besides zirconium-based cladding, versatile accidental tolerant fuel (ATF) claddings, including FeCrAl, have been proposed to increase the accident coping time. In order to investigate the effect of surface properties on droplet impact cooling of cladding surfaces, the droplet impact phenomena are photographed on the FeCrAl and zircaloy-4 (Zr-4) surfaces under different conditions. On the oxidized FeCrAl surface, the Leidenfrost phenomenon is not observed even when the surface temperature is as high as 550 ℃ with We > 30. Comparison of the impact behaviors observed on different materials shows that nucleate and transition boiling is more intensive on surfaces with larger thermal conductivity. The Leidenfrost point temperature (LPT) decreases with the solid thermal effusivity (${\sqrt{k{\rho}C_p}}$). However, the CHF temperature is relatively insensitive to the surface oxidation and Weber number. Droplet spreading diameter is analyzed quantitatively in the film boiling stage. Based on the energy balance a correlation is proposed for droplet maximum spreading factor. A mechanistic model is also developed for the LPT based on homogeneous nucleation theory.

A Two-DNA Methylation Signature to Improve Prognosis Prediction of Clear Cell Renal Cell Carcinoma

Shanping Shi,Shazhou Ye,Xiaoyue Wu,Mingjun Xu,Renjie Zhuo,Qi Liao,Yang Xi 연세대학교의과대학 2019 Yonsei medical journal Vol.60 No.11

Purpose: Effective biomarkers and models are needed to improve the prognostic prospects of clear cell renal cell carcinoma(ccRCC). The purpose of this work was to identify DNA methylation biomarkers and to evaluate the utility of DNA methylationanalysis for ccRCC prognosis. Materials and Methods: An overview of genome-wide methylation of ccRCC tissues derived from The Cancer Genome Atlas(TCGA) database was download for analysis. DNA methylation signatures were identified using Cox regression methods. The potentialclinical significance of methylation biomarkers acting as a novel prognostic markers was analyzed using the Kaplan–Meiermethod and receiver operating characteristic (ROC) curves. Results: This study analyzed data for 215 patients with information on 23171 DNA methylation sites and identified a two-DNAmethylation signature (cg18034859, cg24199834) with the help of a step-wise multivariable Cox regression model. The area underthe curve of ROCs for the two-DNA methylation signature was 0.819. The study samples were stratified into low- and high-risk classificationsbased on an optimal threshold, and the two groups showed markedly different survival rates. Moreover, the two-DNAmethylation marker was suitable for patients of varying ages, sex, stages (I and IV), and histologic grade (G2). Conclusion: The two-DNA methylation signature was deemed to be a potential novel prognostic biomarker of use in increasingthe accuracy of predicting overall survival of ccRCC patients.

Density measurement and equal density temperature of CO2+brine from Dagang - formation from 313 to 363 K

Yi Zhang,Weiwei Jian,Yangchun Zhan,Yongchen Song,Mingjun Yang,Jiafei Zhao,Yu Liu,Weiguo Liu,Yong Shen 한국화학공학회 2015 Korean Journal of Chemical Engineering Vol.32 No.1

Densities of CO2+Dagang - formation brine solution were measured by a magnetic suspension balance(MSB) in the pressure range from (10 to 18) MPa, at the temperatures from (313.15 to 363.15) K and CO2 mass frac-tions at 0, 0.0101, 0.0198 and 0.0299. The experimental results revealed that the solution densities increased linearlywith the increasing pressure and CO2 concentration, while decreasing with the increasing temperatures in the experi-mental range. When the temperature increased from (313.15 to 363.15) K, the slopes of the densities versus (vs.) CO2mass fractions decreased from (0.193 to 0.106) g·cm−3. A correlation equation was developed based on thermody-namic theory and experimental data. The absolute average deviation between the correlation equation and the experi-mental data was 0.05%, and the maximum deviation was 0.37% for the density of CO2+water/brine solution in com-mon geological storage conditions. According to the density of CO2 - free brine and apparent molar volume of CO2 inbrine, the equal density temperature (Te) of CO2+Dagang brine solution was obtained at 464.67 K when pressure is10MPa, which means that the density of brine dissolved with CO2 will be less than that of CO2-free brine when thetemperature is higher than 464.67 K at 10MPa. In this work the formation temperature of the Dagang oilfield reser-voir is from 313.15 K to 363.15 K, which is lower than the equal density temperature. Therefore, the safety of CO2 stor-age in Dagang oilfield reservoir can be guaranteed.

Lithiation of an Iron Oxide-Based Anode for Stable, High-Capacity Lithium-Ion Batteries of Porous Carbon-Fe₃O₄/Li[Ni_0.59Co_0.16Mn_0.25]O₂

Ming, Jun,Kwak, Won Jin,Youn, Sung Jun,Ming, Hai,Hassoun, Jusef,Sun, Yang-Kook Wiley (John WileySons) 2014 Energy technology Vol.2 No.9

The binder effect on an oxide-based anode in lithium and sodium-ion battery applications: the fastest way to ultrahigh performance

Ming, Jun,Ming, Hai,Kwak, Won-Jin,Shin, Changdae,Zheng, Junwei,Sun, Yang-Kook The Royal Society of Chemistry 2014 Chemical communications Vol.50 No.87

<P>A positive effect of the polyacrylic acid (PAA)–carboxymethyl cellulose (CMC) binder to enhance the performance of an oxide-based anode was reported in batteries. A series of super high capacity and cycling ability oxide powders rarely achieved before was obtained, particularly most of them without any specific carbon modification and/or morphology control.</P> <P>Graphic Abstract</P><P>A positive effect of the polyacrylic acid–carboxymethyl cellulose binder to enhance the performance of an oxide-based anode was reported in batteries. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c4cc02657h'> </P>

Fine control of titania deposition to prepare C@TiO₂ composites and TiO₂ hollow particles for photocatalysis and lithium-ion battery applications

Ming, Jun,Wu, Yingqiang,Nagarajan, Srinivasan,Lee, Dong-Ju,Sun, Yang-Kook,Zhao, Fengyu The Royal Society of Chemistry 2012 Journal of materials chemistry Vol.22 No.41

<P>In this study, an effective method of slow hydrolization of metal alkoxide (<I>e.g.</I>, Ti(C<SUB>4</SUB>H<SUB>9</SUB>O)<SUB>4</SUB>) in an ethanol–water system was systematically investigated and used to finely control the deposition of titania on carbon colloids. A model of adsorption–hydrolization of precursors during the coating process was rationally built for the first time to interpret the usability of the method and facilitate its further extension. Using this strategy, titania in the form of supported nanocrystals or layers on carbon colloids (TiO<SUB>2</SUB>/C, C@TiO<SUB>2</SUB>) was successfully tailored. Meanwhile, finely dispersed hollow TiO<SUB>2</SUB> nanoparticles with shells consisting of different crystalline structures were also prepared by varying the calcination conditions after removing the carbon cores. More importantly, the effects of the crystalline and nano/macrostructures of the as-prepared TiO<SUB>2</SUB> samples in photocatalysis and lithium-ion battery applications were analyzed in detail. The preliminary results show that anatase–rutile TiO<SUB>2</SUB> hollow particles demonstrate a higher catalytic activity in the photo-degradation of rhodamine B than anatase TiO<SUB>2</SUB> hollow particles, powders, and P25. However, in the case of Li-ion battery applications, the anatase TiO<SUB>2</SUB> hollow particles exhibited better performance as anode materials with high capacities of around 190 mA h g<SUP>−1</SUP>, 140 mA h g<SUP>−1</SUP>, and 120 mA h g<SUP>−1</SUP> at current densities of 60 mA g<SUP>−1</SUP>, 120 mA g<SUP>−1</SUP>, and 300 mA g<SUP>−1</SUP>, respectively, accompanied by stable cyclability.</P> <P>Graphic Abstract</P><P>Fine control of the titania deposition to prepare C@TiO<SUB>2</SUB> composites and TiO<SUB>2</SUB> hollow particles for photocatalysis and lithium-ion battery applications. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c2jm34106a'> </P>