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Karyotype Analysis and Tandem Repeats in Heterochromatin of Brassica rapa
Ki-Byung Lim(임기병),Tae-Jin Yang(양태진),Jee-Young Park(박지영),Soo-Jin Kwon(권수진),JungSun Kim(김정선),Myung-Ho Lim(임명호),JinA Kim(김진아),Mina Jin(진민아),Yong-Moon Jin(진용문),Seog Hyung Kim(김석형),YP Lim(임용표),JW Bang(방재욱) 한국원예학회 2005 한국원예학회 학술발표요지 Vol.2005 No.5
EMS 유도 내염성 증진 사료용 옥수수 돌연변이체 선발 및 특성 분석
조철오,김경화,서미숙,최만수,전재범,진민아,김둘이,Cho, Chuloh,Kim, Kyung Hwa,Seo, Mi-Suk,Choi, Man-Soo,Chun, Jaebuhm,Jin, Mina,Kim, Dool-Yi 한국작물학회 2020 한국작물학회지 Vol.65 No.4
Maize (Zea mays L.) is one of the most valuable agricultural crops and is grown under a wide spectrum of environmental conditions. However, maize is moderately sensitive to salt stress, and soil salinity is a serious threat to its production worldwide. In this study, we used ethyl methane sulfonate (EMS) to generate salt-tolerant silage maize mutants. We screened salt-tolerant lines from 203 M<sub>3</sub> mutant populations by evaluating the morphological phenotype after salt stress treatment and selected the 140ES91 line. The 140ES91 mutant showed improved plant growth as well as higher proline content and leaf photosynthetic capacity compared with those of wild-type plants under salt stress conditions. Using whole-genome re-sequencing analysis, 1,103 single nucleotide polymorphisms and 71 insertions or deletions were identified as common variants between KS140 and 140ES91 in comparison with the reference genome B73. Furthermore, the expression patterns of three genes, which are involved in salt stress responses, were increased in the 140ES91 mutant under salt stress. Taken together, the mutant line identified in our study could be used as an improved breeding material for transferring salt tolerance traits in maize varieties.
한국 콩(Glycine max (L.) Merr) 품종의 효율적인 원형질체 분리 및 유전자 도입
조철오(Chuloh Cho),김둘이(Dool-Yi Kim),최만수(Man-Soo Choi),진민아(Mina Jin),서미숙(Mi-Suk Seo) 한국육종학회 2021 한국육종학회지 Vol.53 No.3
Soybean (Glycine max (L.) Merr.) is one of the most important crops with economic value as a source of protein and vegetableoil for human food and animal feed. In recent years, rapidly developed genome editing techniques have shown widespread application prospectsfor gene function studies and for improving important agronomic traits in many crops. Therefore, it is important to establish a highly efficientmethod for protoplast isolation and transient expression systems in soybeans. In this study, we established an efficient method for protoplastisolation and its application to transient gene expression in Korean soybean cultivars. The protoplasts were isolated from leaves, epicotyls,hypocotyls, cotyledons, and etiolated hypocotyls using various combinations of enzyme mixtures. We found that high-quality and large amountsof protoplasts were isolated from the etiolated hypocotyls when incubated for 8 h under conditions of 0.5% cellulase, 0.5% pectinase, and1% viscozyme. In addition, we observed a high transfection efficiency of green fluorescent protein using etiolated hypocotyl protoplasts. Takentogether, our protoplast isolation and transfection method is highly efficient and can be used for gene function and molecular analysis tobetter understand the biological and physiological processes in soybean.
서미숙(Mi-Suk Seo),조철오(Chuloh Cho),정남희(Namhee Jeong),성순기(Soon-Kee Sung),최만수(Man-Soo Choi),진민아(Mina Jin),김둘이(Dool-Yi Kim) 한국자원식물학회 2021 한국자원식물학회지 Vol.34 No.4
유전자 가위 기술 등 생명공학 기술을 콩에 적용하여 새로운품종을 개발하기 위해서는 효율적인 조직배양 기술이 필수적이다. 식물의 유전형은 조직배양 효율에 의존하는 형질전환 기술의 성공 여부를 결정짓는 중요한 요소로 알려져 있다. 본 연구에서는 우리나라 콩 핵심 집단 내 21개 자원들을 선발하여, 외래품종 2종과 함께 조직배양 효율을 조사하였다. 그 결과, 근연 관계가 높은 Kwangan, Anpyeong, Seonam은 발아율과 재분화효율이 높았으며, Daepung, Daewon 품종은 발아율과 재분화율 모두 낮게 관찰되었다. 또한 3종의 외래 품종에서는 표준 유전체 해독에 사용된 Williams82와 Jack, Maverick 모두 높은조직배양 효율을 보였다. 조직배양 효율이 높은 자원들을 대상으로 Agrobacterium법에 의한 형질전환을 수행하여 PCR 및bar-strip 분석한 결과 Kwangan, Pungwon, Seonam, 그리고Maverick 품종에서 제초제 저항성 유전자의 삽입을 확인할 수있었다. 이들 결과를 바탕으로 농업적 가치가 높은 다양한 콩 품종들의 형질전환을 통한 새로운 품종 개발이 가능할 것이다. Efficient in vitro regeneration system is essential for the successful crop breeding of soybean (Glycine max (L.) Merr.) using the new biotechnology. The genotype of donor plants strongly influences the establishment of tissue culture system. Therefore, the screening of genotypes with excellent tissue culture ability is very important for soybean genetic improvement. In this study, we report the tissue culture efficiency of 21 soybean cultivars belong to Korean soybean core-collection and two foreign cultivars (Jack and Maverick). The Kwangan, Anpyeong and Seonam are share close genetic relationship in 21 cultivars and these three cultivars were observed the high frequency of germination and regeneration. Furthermore, the high tissue culture abilities were also observed in the Williams 82 used in reference genome sequencing and the two foreign cultivars. The transformation of pBAtc:tRNA with bar gene was performed by Agrobacterium tumefaciens in the cultivars with high tissue culture ability. Transformation of the bar gene was identified by PCR analysis in Kwangan, Pungwon, Seonam, and Maverick. Our results provide useful information for the breeding of various soybean cultivars by plant biotechnology such as, genome editing.