FSCB phosphorylation in mouse spermatozoa capacitation

( Shun Li Liu ),( Bing Ni ),( Xiang Wei Wang ),( Wen Qian Huo ),( Jun Zhang ),( Zhi Qiang Tian ),( Ze Min Huang ),( Yi Tian ),( Jun Tang ),( Yan Hua Zheng ),( Feng Shuo Jin ),( Yan Feng Li ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.8

It is generally accepted that spermatozoa capacitation is associated with protein kinase A-mediated tyrosine phosphorylation. In our previous study, we identified the fibrous sheath CABYR binding protein (FSCB), which was phosphorylated by PKA. However, the phosphorylation status of FSCB protein during spermatozoa capacitation should be further investigated. To this aim, in this study, we found that phosphorylation of this 270-kDa protein occurred as early as 1 min after mouse spermatozoa capacitation, which increased over time and remained stable after 60 min. Immunoprecipitation assays demonstrated that the tyrosine and Ser/Thr phosphorylation of FSCB occurred during spermatozoa capacitation. The extent of phosphorylation and was closely associated with the PKA activity and spermatozoa motility characteristics. FSCB phosphorylation could be induced by PKA agonist DB-cAMP, but was blocked by PKA antagonist H-89.Therefore, FSCB contributes to spermatozoa capacitation in a tyrosine-phosphorylated format, which may help in further elucidating the molecular mechanism of spermatozoa capacitation. [BMB reports 2011; 44(8): 541-546]

Comparison of ¹⁸F-Labeled Fluoroalkylphosphonium Cations with ¹³N-NH₃ for PET Myocardial Perfusion Imaging

Kim, Dong-Yeon,Kim, Hyeon Sik,Reder, Sybille,Zheng, Jin Hai,Herz, Michael,Higuchi, Takahiro,Pyo, AYoung,Bom, Hee-Seung,Schwaiger, Markus,Min, Jung-Joon Society of Nuclear Medicine 2015 The Journal of nuclear medicine Vol.56 No.10

<P>Despite substantial advances in the diagnosis of cardiovascular disease, there is a need for <SUP>18</SUP>F-labeled myocardial perfusion agents for the diagnosis of ischemic heart disease because current PET tracers for myocardial perfusion imaging have a short half-life that limits their widespread clinical use in PET. Thus, <SUP>18</SUP>F-labeled fluoroalkylphosphonium derivatives (<SUP>18</SUP>F-FATPs), including (5-<SUP>18</SUP>F-fluoropentyl)triphenylphosphonium cation (<SUP>18</SUP>F-FPTP), (6-<SUP>18</SUP>F-fluorohexyl)triphenylphosphonium cation (<SUP>18</SUP>F-FHTP), and (2-(2-<SUP>18</SUP>F-fluoroethoxy)ethyl)triphenylphosphonium cation (<SUP>18</SUP>F-FETP), were synthesized. The myocardial extraction and image quality of the <SUP>18</SUP>F-FATPs were compared with those of <SUP>13</SUP>N-NH<SUB>3</SUB> in rat models. <B>Methods:</B> The first-pass extraction fraction (EF) values of the <SUP>18</SUP>F-FATPs (<SUP>18</SUP>F-FPTP, <SUP>18</SUP>F-FHTP, <SUP>18</SUP>F-FETP) and <SUP>13</SUP>N-NH<SUB>3</SUB> were measured in isolated rat hearts perfused with the Langendorff method (flow velocities, 0.5, 4.0, 8.0, and 16.0 mL/min). Normal and myocardial infarction rats were imaged with small-animal PET after intravenous injection of 37 MBq of <SUP>18</SUP>F-FATPs and <SUP>13</SUP>N-NH<SUB>3.</SUB> To determine pharmacokinetics, a region of interest was drawn around the heart, and time–activity curves of the <SUP>18</SUP>F-FATPs and <SUP>13</SUP>N-NH<SUB>3</SUB> were generated to obtain the counts per pixel per second. Defect size was analyzed on the basis of polar map images of <SUP>18</SUP>F-FATPs and <SUP>13</SUP>N-NH<SUB>3.</SUB> <B>Results:</B> The EF values of <SUP>18</SUP>F-FATPs and <SUP>13</SUP>N-NH<SUB>3</SUB> were comparable at low flow velocity (0.5 mL/min), whereas at higher flows EF values of <SUP>18</SUP>F-FATPs were significantly higher than those of <SUP>13</SUP>N-NH<SUB>3</SUB> (4.0, 8.0, and 16.0 mL/min, <I>P</I> < 0.05). Myocardium-to-liver ratios of <SUP>18</SUP>F-FPTP, <SUP>18</SUP>F-FHTP, <SUP>18</SUP>F-FETP, and <SUP>13</SUP>N-NH<SUB>3</SUB> were 2.10 ± 0.30, 4.36 ± 0.20, 3.88 ± 1.03, and 0.70 ± 0.09, respectively, 10 min after injection, whereas myocardium-to-lung ratios were 5.00 ± 0.25, 4.33 ± 0.20, 7.98 ± 1.23, and 2.26 ± 0.14, respectively. Although <SUP>18</SUP>F-FATPs and <SUP>13</SUP>N-NH<SUB>3</SUB> sharply delineated myocardial perfusion defects, defect size on the <SUP>13</SUP>N-NH<SUB>3</SUB> images was significantly smaller than on the <SUP>18</SUP>F-FATP images soon after tracer injection (0–10 min, <I>P</I> = 0.027). <B>Conclusion:</B> <SUP>18</SUP>F-FATPs exhibit higher EF values and more rapid clearance from the liver and lung than <SUP>13</SUP>N-NH<SUB>3</SUB> in normal rats, which led to excellent image quality in a rat model of coronary occlusion. Therefore, <SUP>18</SUP>F-FATPs are promising new PET radiopharmaceuticals for myocardial perfusion imaging.</P>

Stability of Aperiodic Sampled-data Systems - An Augmented Looped Lyapunov Functional with Wirtinger Inequality Approach

Min Zheng,Hainan Yang,Yang Song,Jing Deng,Minrui Fei 제어·로봇·시스템학회 2015 International Journal of Control, Automation, and Vol.13 No.3

This paper is concerned with stability analysis of sampled-data systems with non-uniform sampling patterns. The stability problem is solved based on an augmented looped Lyapunov functional with Wirtinger inequality approach. The effectiveness of this approach depends on proper selecting looped Lyapunov functional parts and less conservative inequality techniques. The method developed here is an extension of previous results by using novel augmented functional and Wirtinger inequality. Numerical examples are given to illustrate the result.

Development and validation of modified QuEChERS method coupled with LC–MS/MS for simultaneous determination of cymiazole, fipronil, coumaphos, fluvalinate, amitraz, and its metabolite in various types of honey and royal jelly

Zheng, Weijia,Park, Jin-A.,Abd El-Aty, A.M.,Kim, Seong-Kwan,Cho, Sang-Hyun,Choi, Jeong-min,Yi, Hee,Cho, Soo-Min,Ramadan, Amer,Jeong, Ji Hoon,Shim, Jae-Han,Shin, Ho-Chul Elsevier 2018 Journal of chromatography. B, Analytical technolog Vol.1072 No.-

<P><B>Abstract</B></P> <P>Over the past few decades, honey products have been polluted by different contaminants, such as pesticides, which are widely applied in agriculture. In this work, a modified EN – quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction method was developed for the simultaneous quantification of pesticide residues, including cymiazole, fipronil, coumaphos, fluvalinate, amitraz, and its metabolite 2,4-dimethylaniline (2,4-DMA), in four types of honey (acacia, wild, chestnut, and manuka) and royal jelly. Samples were buffered with 0.2M dibasic sodium phosphate (pH 9), and subsequently, acetonitrile was employed as the extraction solvent. A combination of primary secondary amine (PSA) and C18 sorbents was used for purification prior to liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI<SUP>+</SUP>/MS-MS) analysis. The estimated linearity measured at six concentration levels presented good correlation coefficients (<I>R<SUP>2</SUP> </I>)≥0.99. The recovery, calculated from three different spiking levels, was 62.06–108.79% in honey and 67.58–106.34% in royal jelly, with an RSD<12% for all the tested compounds. The matrix effect was also evaluated, and most of the analytes presented signal enhancement. The limits of quantification (LOQ) ranged between 0.001 and 0.005mg/kg in various samples. These are considerably lower than the maximum residue limits (MRL) set by various regulatory authorities. A total of 43 market (domestic and imported) samples were assayed for method application. Among the tested samples, three samples were tested positive (i.e. detected and quantified) only for cymiazole residues. The residues in the rest of the samples were detected but not quantified. We concluded that the protocol developed in this work is simple and versatile for the routine quantification of cymiazole, 2,4-DMA, fipronil, coumaphos, amitraz, and fluvalinate in various types of honey and royal jelly.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Six pesticide residues in four types of honey and royal jelly were determined by LC–MS/MS analysis. </LI> <LI> Samples were extracted by a modified EN-QuEChERS method using both C18 sorbent and PSA. </LI> <LI> Recovery was good when the honey samples were extracted with 0.2M dibasic sodium phosphate buffer and acetonitrile. </LI> <LI> Three of the market samples tested positive for cymiazole residues. </LI> </UL> </P>

Sensing endoplasmic reticulum stress by protein kinase RNA‐like endoplasmic reticulum kinase promotes adaptive mitochondrial DNA biogenesis and cell survival <i>via</i> heme oxygenase‐1/carbon monoxide activity

Zheng, Min,Kim, Seul‐,Ki,Joe, Yeonsoo,Back, Sung Hoon,Cho, Hong R.,Kim, Hong Pyo,Ignarro, Louis J.,Chung, Hun‐,Taeg Federation of American Society for Experimental Bi 2012 The FASEB Journal Vol.26 No.6

<P>Endoplasmic reticulum (ER) stress activates the adaptive unfolded protein response, allowing cells to recover folding capacity in the organelle. However, the overwhelming response to severe damage results in apoptotic cell death. Because of the physical proximity between ER and mitochondria, a functional interrelationship between these two organelles, including mitochondrial ATP production and apoptosis, has been suggested. The adaptive response to ER stress includes the maintenance of cellular energetics, which eventually determines cell fate. We previously demonstrated that heme oxygenase-1 (HO-1) activity protects cells against ER stress in a protein kinase RNA-like endoplasmic reticulum kinase (PERK)-dependent pathway. Here, we provide evidence that PERK-mediated induction of HO-1 in murine macrophages, RAW264.7, relays ER stress to mitochondrial DNA (mtDNA) replication and function. ER stress induced by thapsigargin treatments (10-100 nM) resulted in a 2-fold increase in mtDNA contents compared with that in the untreated control. HO-1 activity on ER stress is proven to be critical for mitochondrial integrity because chemical inhibition (zinc protoporphyrin, 5-20 관M) and genetic depletion of HO-1 by small interference RNA transfection suppress the activation of transcription factors for mitochondrial biogenesis. Carbon monoxide (CO), an enzymatic by-product of HO-1 activity is responsible for the function of HO-1. Limited bioavailability of CO by hemoglobin treatment triggers cell death with a concomitant decline in ATP production. Approximately 78.1% of RAW264.7 cells were damaged in the presence of hemoglobin compared with the percentage of injured cells (26.9%) under ER stress alone. Mitochondrial generation of ATP levels significantly declined when CO availability was limited under prolonged ER stress. Taken together, these results suggest that the cellular HO-1/CO system conveys ER stress to cell survival signals from mitochondria via both the activation of transcriptional factors and functional integrity of mtDNA.</P>

Injection Molding of Carbon Fiber Composite Automotive Wheel

Zheng Min Huang,김형민,윤재륜,송영석 한국섬유공학회 2019 Fibers and polymers Vol.20 No.12

Injection molding (IM) is a manufacturing process that produces polymeric parts by injecting molten thermoplasticpolymer into a mold cavity. It mainly fabricates geometrically complicated parts with dimensional accuracy. In this paper,injection molding of carbon fiber composite automotive wheels with complex shape was investigated numerically. Insertinjection molding was also considered to reduce the deformation of the part. Injection molding conditions were determinedbased on the simulation results such as filling time, mechanical property, and warpage for manufacturing of compositeautomotive wheels.

Three-Dimensional Numerical Analysis of Resin Transfer Molding for CFRP Composite Rim

Zheng Min Huang(황정민),So Yun Lee(이소윤),Hyung Min Kim(김형민),Young Seok Song(송영석),Jae Ryoun Youn(윤재륜) 대한기계학회 2016 대한기계학회 춘추학술대회 Vol.2016 No.4

Bithionol residue analysis in animal-derived food products by an effective and rugged extraction method coupled with liquid chromatography–tandem mass spectrometry

Zheng, Weijia,Park, Jin-A,Abd El-Aty, A.M.,Kim, Seong-Kwan,Cho, Sang-Hyun,Choi, Jeong-Min,Yi, Hee,Cho, Soo-Min,El-Banna, H.A.,Shim, Jae-Han,Chang, Byung-Joon,Wang, Jing,Kim, Jin-Suk,Shin, Ho-Chul Elsevier 2017 Journal of chromatography. B, Analytical technolog Vol.1064 No.-

<P><B>Abstract</B></P> <P>Herein, we developed a simple analytical procedure for the quantitation of bithionol residues in animal-derived food products such as porcine muscle, eggs, milk, eel, flatfish, and shrimp using a modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction method coupled with liquid chromatography–electrospray ionization tandem mass spectrometry (LC-ESI<SUP>+</SUP>/MS-MS). Samples were extracted with 0.1% solution of formic acid in acetonitrile and the extract was purified using a C18 sorbent. Separation was performed on a Waters XBridge™ C18 reversed-phase analytical column using 0.1% solution of formic acid/acetonitrile as the mobile phase. Six-point matrix-matched calibration indicated good linearity, with the calculated coefficients of determination (<I>R</I> <SUP>2</SUP>) being≥0.9813. Intra- and inter-day recoveries (determined at spiking levels equivalent to 1×and 2×the limit of quantitation (0.25μg/kg)) ranged between 80.0 and 94.0%, with the corresponding relative standard deviations (RSDs) being≤8.2%. The developed experimental protocol was applied to different samples purchased from local markets in Seoul, which were tested negative for bithionol residues. In conclusion, the proposed method proved to be versatile and precise, being ideally suited for the routine detection of bithionol residues in animal-derived food products with various protein and fat contents.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Bithionol residues were quantified in porcine muscle, whole milk, eggs, eel, flatfish, and shrimp. </LI> <LI> Samples were extracted using a modified EN-QuEChERS method followed by LC–MS/MS analysis. </LI> <LI> Satisfactory extraction efficiency was obtained using 0.1% formic acid in acetonitrile as an extractant. </LI> <LI> None of the market samples tested positive for the target analyte. </LI> <LI> The developed method can help establish the MRL of bithionol, which is yet to be set by regulatory authorities. </LI> </UL> </P>

How to improve the survival rate of implants after radiotherapy for head and neck cancer?

Zheng, Min,Li, Li,Tang, Yaling,Liang, Xin-Hua Korean Academy of Periodontology 2014 Journal of Periodontal & Implant Science Vol.44 No.1

Implants have been widely used in restorative treatment for patients who have undergone head and neck cancer surgery. With the development of combination treatment of head and neck cancer, radiotherapy has been a common means of therapy. However, it could induce various changes in hard and soft tissues and reduce the success and survival rate of the implants. Some research, using either animal models or clinical studies, have shown that certain strategies could be used for improving the survival rate of implants. In this review, we discussed the changes in both hard and soft tissues, which may reduce the survival rate of the implants, and the proposed methods for improving the survival rate of patients after radiotherapy.

Permeability Measurement of Plain Woven Fabric for Resin Transfer Molding

Zheng Min Huang,Hyung Min Kim,Young Seok Song,Jae Ryoun Youn 대한기계학회 2015 대한기계학회 춘추학술대회 Vol.CAE 및 응용역학 No.-