Computational Protein Engineering

Costas Maranas 한국생물공학회 2007 한국생물공학회 학술대회 Vol.2007 No.10

Designing the substrate specificity of d-hydantoinase using a rational approach

Lee, S.C.,Chang, Y.,Shin, D.M.,Han, J.,Seo, M.H.,Fazelinia, H.,Maranas, C.D.,Kim, H.S. IPC Science and Technology Press ; Elsevier Scienc 2009 Enzyme and microbial technology Vol.44 No.3

Enzymes that exhibit superior catalytic activity, stability and substrate specificity are highly desirable for industrial applications. These goals prompted the designed substrate specificity of Bacillus stearothermophilusd-hydantoinase toward the target substrate hydroxyphenylhydantoin (HPH). Positions crucial to substrate specificity were selected using structural and mechanistic information on the structural loops at the active site. The size and hydrophobicity of the involved amino acids were rationally changed, and the substrate specificities of the designed d-Hyd mutants were investigated. As a result, M63I/F159S exhibited about 200-fold higher specificity for HPH than the wild-type enzyme. Systematic mutational analysis and computational modeling also supported the rationale used in the design.

Temperature-dependent development of overwintered eggs of Lycorma delicatula (Hemiptera: Fulgoridae)

Marana Park,Se-jin Kim,Yong-Joon Kwon,Ju-Won Yoo,Joon-Ho Lee 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.05

We studied to develop a forecasting model to predict the hatching time of overwintered eggs of Lycorma delicatula. We collected overwintering egg masses on 1st February, 17th February and 4th March. After chilling them at 5℃ for 15 days, development of eggs was investigated at six constant temperature (35, 31, 27, 23, 19, 15℃). The hatching rate of egg was highest at 23℃ (87.88±19.32%) followed by 19℃ (87.71±21.47%), 27℃ (75.96±24.82%), and 31℃ (30.92±24.81%). Eggs did not survive at 35℃. The developmental duration of eggs was 39.47±2.24, 22.96±3.25, 17.56±1.58 and 12.15±6.29, at 19, 23, 27 and 31℃, respectively. The egg developmental rate was described with a linear model (eq. Y=0.0034X-0.0364 (r=0.9649)) between 19℃ and 31℃. The lower developmental threshold temperature was 10.71℃ and effective accumulated temperature for egg development was 286.40 Degree days.

Cold hardiness of overwintering eggs of five Lycorma delicatula (White) populations in South Korea.

Marana Park,Yong-Joon Kwon,Ju-won Yoo,Joon-Ho Lee 한국응용곤충학회 2011 한국응용곤충학회 학술대회논문집 Vol.2011 No.05

Cold hardiness is an important trait for insects, that enables them to survive during the winter and develop in the next season, and to extend their range. Lycorma delicatula (White), which became an important sporadic pest in grapevines and some other fruit trees, has spread rapidly to the most of regions in South Korea. This study was conducted to determine the cold hardiness of overwintered L. delicatula eggs according to geographical variation. We collected overwintering eggs in the five sites, Chuncheon, Suwon, Yeongdong, Gunsan, Daegu, on 22~25th Feb. in 2011. We treated eggs to combinations of different temperatures (-15℃, -20℃, -25℃) and exposure time (12hr, 24hr, 3days, 5days, 7days) after chilling them for 24~26 days at 5℃. And then, they were kept in the room temperature. We only analysed three sites of Suwon, Gunsan and Daegu, because of very low hatching rate at Chuncheon and Yeongdong. There were significant effects of time (F=36.97 d.f.=4 P<0.0001), sites (F=17.28 d.f.=2 P<0.0001), and the interaction of time and temperature (F=5.77 d.f.=5 P<0.0001) at -15℃. At -20℃, eggs hatched only at 12hr and 24hr of exposure treatment. All eggs treated at -25℃ failed to hatch. It appears that -25℃ is around the critical temperature.

Isolation and characterization of eight microsatellite loci from Lycorma delicatula (White) (Hemiptera: Fulgoridae) for population genetic analysis in Korea

Park, Marana,Kim, Kyung-Seok,Lee, Joon-Ho Springer-Verlag 2012 Molecular biology reports Vol.39 No.5

전남 무안 승달산 나비목 곤충의 생물다양성 연구

박마라나 ( Marana Park ),오상기 ( Sang Gi Oh ),안정섭 ( Jeong Seop An ),김권일 ( Kwon Il Kim ),최세웅 ( Sei Woong Choi ) 한국환경생물학회 2007 환경생물 : 환경생물학회지 Vol.25 No.1

Diversity of Moths (Insecta: Lepidoptera) on Bogildo Island, Wando-gun, Jeonnam, Korea

Park, Marana,An, Jeong-Seop,Lee, Jin,Lim, Jin-Taek,Choi, Sei-Woong The Ecological Society of Korea 2009 Journal of Ecology and Environment Vol.32 No.2

We investigated the moth diversity on an island of southern sea of Korea. We collected moths at three sites on the island of Bogildo, Wando-gun, Jeonnam using a 22-watt ultraviolet light trap from May to October, 2008, and identified a total of 272 species and 948 individuals in 13 families. Species of Noctuidae was the most abundant, with 107 species and 318 individuals, followed by Geometridae (62 species and 147 individuals) and Pyralidae (53 species and 269 individuals). The graph of the estimated species richness in Chao 1 (432.25$\pm$37.39) did not reach an asymptote, which suggests that more moth species could be identified on the island through further sampling. An arctiid moth, Miltochrista striata, was the most abundant species captured in this study. Monthly changes in moth species richness and abundance formed M-shaped curves, with peaks in early summer (June) and late summer (August). Cluster analysis of seven sites on three islands (Aphaedo Island, Sinan-gun, Oenarodo Island, Goheung-gun and Bogildo Island) divided the sites into two groups. Distances among sites and habitat types may play an important role in determining the similarities of moth faunas among sites.

예비 환경교사들의 봉사학습 경험 및 의미 탐색

박마라나(Marana Park),장영효(Young Hyo Chang),김상채(Sang Chai Kim) 한국환경교육학회 2020 環境 敎育 Vol.33 No.1

본 연구를 통해 환경교육 비교과 활동의 교육적 함의를 살펴보고, 예비 환경교사 교육 프로그램으로 활용 가능성을 모색하였다. 본 연구결과를 정리하면 다음과 같다. 첫째, 환경교육학습동아리는 학생 자치활동으로 독립적 · 자율적인 학습을 통해 전공에 대한 새로운 인식과 진로탐색의 기회가 되었다. 둘째, 환경교육학습공동체는 구성원들이 환경교육 활동을 위해 상호협력을 통해 공동체의 환경교육 역량이 발달하였고, 공동체의 학습 역량을 지역사회로 전달하는 역할에 중요한 의의가 있다. 셋째, 환경교육 봉사학습 경험을 통해 예비 환경교사들은 실천적 지식을 형성하였다. 예비환경교사의 봉사학습 경험은 실천적 지식의 5가지 외적 요소(교과내용지식, 학생들과의 관계에 대한 지식, 학생들의 학습을 위한 교수법적 지식, 환경교육 교수-학습에 대한 지식, 환경교육 현장에 대한 지식)를 형성하며, 환경교육 역량과 전문성이 발달되었다. 또한, 교사로서 자아에 대한 성찰 기회를 통해 실천적 지식의 내적 요소가 형성되었으며, 이는 외적 요소와 상호작용하며 발현함을 알 수 있었다. 또한, 봉사학습 운영 사례 제시를 통해 교과활동과 연계된 봉사학습 전환과 이를 교육봉사활동으로 연계할 수 있는 방안에 대해 제안하였다. In this study, we were explored the availability of education program for pre-service environmental education teacher by examining the extracurricular activities. The results of this study are summarized as follows. First, the environmental education learning circle was an autonomous activity by students, and it became an opportunity for new recognition and career exploration of environmental education. Second, the environmental education learning community has significance meaning by delivering the capabilities of environmental education into the local community, and environmental education competency was developed through mutual cooperation for education activities. Third, pre-service teachers have formed PPK (Personal Practical Knowledge) through environmental education experience in service learning. The pre-service teacher were formed 5 external factors of PPK (subject matter content knowledge, knowledge about the relationship with students, pedagogical knowledge, instruction knowledge of environmental education, knowledge about the environmental education in field) by experiencing the service learning. Through the development of external factors, environmental education competency and expertise were developed. And, the internal factor of PPK was formed by recognizing the knowledge of oneself as a teacher, and this was expressed by interacting the external factors. In addition, we proposed the service learning linked to curriculum activities and further educational service.

Differences in Moth Diversity in Two Types of Forest Patches in an Agricultural Landscape in Southern Korea - Effects of Habitat Heterogeneity -

Choi, Sei-Woong,Park, Marana,Kim, Hui The Ecological Society of Korea 2009 Journal of Ecology and Environment Vol.32 No.3

This research focused on the effects of fragmentation on moth diversity in an agricultural land-scape by comparing moth species richness and abundance between hillocks and young secondary forests. We examined five sites from the southwestern part of South Korea: three sites from hillock forest and two from secondary forest. We collected moths bi-weekly from April to October for a 2-year period (2006-2007) with a UV light trap that usually attracts moths within 30 meters. Tree species richness and abundance in $20m{\times}20m$ plots at each moth sampling site showed a substantial difference in tree diversity between the two types of forest habitats. The total abundance and richness of moth species were higher in secondary forests (541 species with 4,998 individuals) than in hillock forests (423 species with 3,913 individuals), irrespective of the distance among the sites. An ordination analysis with NMDS showed that habitat is the most important factor of grouping sites. The food preferences of the dominant moth species i,n each habitat were closely related to the habitat type.

Population Changes of Moths (Insecta: Lepidoptera) from Mt. Wolchul National Park, Jeollanam-do, Korea

Choi, Sei-Woong,Park, Marana,Chang, Young-Hyo The Ecological Society of Korea 2007 Journal of Ecology and Environment Vol.30 No.3

We investigated the moth fauna and monthly changes in moth populations at three sites - Dogapsa, Gyungpodae and Muwisa - in Mt. Wolchul National Park, Jeollanam-do, South Korea. From February to October, 2006, we collected a total of 1677 individuals comprising 348 species in 14 families. Monthly changes in the abundance of species and individuals showed an M-shaped pattern, with the highest peak in June and a second high peak in August. The diversity of the three dominant families (Noctuidae, Geometridae and Pyralidae) at the three sites varied, possibly due to differences in vegetation and other environmental factors. Diversity at Dogapsa was relatively higher than Gyungpodae and Muwisa, but, the fauna at Dogapsa more closely resembled Muwisa than Gyungpodae. 28 species occurred at the same time in all three sites, included the families Geometridae (14 species), Noctuidae (9 species), Pyralidae (2 species), Arctiidae (1 species), Nolidae (1 species), and Limacodidae (1 species). The present study provided baseline information about biodiversity and phenological patterns of moth abundance and permitted evaluation of moth biodiversity as a monitoring tool for vegetation structure and environmental change.