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RISS 인기검색어
- 검색키워드
- 저자 : Mahmoud Mustafa (검색결과 9 건)
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Mahmoud Mustafa 대한비뇨의학회 2011 Investigative and Clinical Urology Vol.52 No.3
Purpose: Modest long-term success is one of the most disappointing issues facing patients undergoing anti-incontinence surgery. Herein we introduce a novel surgical modification of the vaginal sling to address the mechanisms that may lead to a reduction in the success rate at the long-term follow-up. Materials and Methods: Twenty-three female patients with mean age of 48.2 years (range, 22-73 years) underwent anti-incontinence surgery to correct their stress urinary incontinence (SUI) between August 2006 and January 2008. The in situ anterior vaginal wall sling, reinforced with equi-size monofilament polypropylene tape, was used as an anti-incontinence surgical procedure. The mean follow-up period was 30.2 months (range, 24-38 months). Results: The surgical technique was successful in 22 patients (95.65%); 20 of them were cured and 2 patients showed clinical improvements. Urinary retention was observed in one patient (4.34%), which was resolved after decreasing the tension of the suspension sutures. No significant post-voiding residue was detected postoperatively. Conclusions: Cost-effectiveness and a low risk of urethral erosion, due to the presence of intervening vaginal mucosa, are important advantages of this technique. Long-term success is expected, because relaxation of the non-tension-free suspension sutures and dislocation of the midurethral sling are less likely.
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Robotic or open radical prostatectomy after previous open surgery in the pelvic region
Mahmoud Mustafa,Curtis A. Pettaway,John W. Davis,Louis Pisters 대한비뇨의학회 2015 Investigative and Clinical Urology Vol.56 No.2
Purpose: We sought to evaluate the feasibility and safety of open or robotic radical prostatectomy (RP) after rectum, sigmoid, orcolon surgery. Materials and Methods: Sixty-four patients with a median age of 65 years (range, 46–73 years) who underwent RP after previouspelvic surgery were included. Twenty-four patients (38%) underwent robotic RP and 40 patients (62%) underwent open RP. Bilaterallymph node dissection and nerve preservation were performed in 50 patients (78%) and 35 patients (55%), respectively. Variablesevaluated included demographic characteristics, perioperative complications, and functional and oncological outcomes. Themedian hospitalization and follow-up periods were 2 days (range, 1–12 days) and 21 months (range, 1–108 months), respectively. Results: No conversions from robotic to open surgery were performed and there were no intraoperative complications. Surgicalmargins were positive in 13 patients (20%), seminal vesicle involvement was detected in 6 patients (9%), and lymph node involvementwas found in 2 patients (3%). Postoperative complications included lymphocele in 1 patient, urethral stricture in 1 patient,and bowel obstruction and persistent bladder leakage in 2 patients. Eighty-eight percent of the patients were continent at 7months and 80% of patients were able to achieve erection with or without medical aid. Conclusions: Open or robotic RP can be done safely and effectively in patients who have previously undergone pelvic surgery. Although prior pelvic surgery of the large intestine was associated with increased morbidity, it should not be considered a contraindicationfor robotic or open RP.
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Mahmoud Mustafa,Rahim Horuz,Metin Celik,Akif Kucukcan 대한비뇨의학회 2014 Investigative and Clinical Urology Vol.55 No.7
Purpose: To evaluate the relationship between levels of total testosterone and totalprostate-specific antigen (PSA) in healthy men with PSA<4 ng/mL. Materials and Methods: The study comprised 179 men with a mean age of 59.19±12years who visited Osmaniye State Hospital, Osmaniye, Turkey, between January 2006and January 2007 for a routine checkup. The patients were divided into two subgroups:patients with PSA<2.5 mg/ml (group I, n=160 patients) and patients with PSA of 2.5to 4 ng/mL (group II, n=19 patients). The relationship between PSA and testosteronelevels was investigated in both groups and in patients aged <60 years. The mean testosteronelevel was calculated for patients aged <50 years and was compared with themean value of patients aged ≥50 years. Results: In all patients, the mean values for serum PSA and total testosterone were1.27±0.88 ng/mL and 404.04±158.86 ng/mL, respectively. No correlation was detectedbetween serum PSA and testosterone levels in either subgroup (group I, r=0.072,p=0.363; group II, r=0.031, p=0.900) or in patients aged <60 years (r=0.032, p=0.72). The mean values of testosterone in patients aged ≥50 years and in patients aged <50years were 417.01±163.35 and 344.16±120.21 ng/dL, respectively (p=0.02). Conclusions: No impact of testosterone was found on the PSA level in healthy men withPSA <4 ng/mL. Therefore, a high serum testosterone level may not mandate adjustmentof PSA values. This serum sex hormone showed a significant increment after theage of 50 years. Further studies including a larger number of patients should be carriedout to confirm these findings.
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Refaie Marwa Monier Mahmoud,Shehata Sayed,El-Hussieny Maram,Fawzy Michael Atef,Ahmed Nagwa Zenhom Mustafa,Marey Heba,Hishmat Asmaa Mohammed,Alkully Turki,Rahman Eman Shaaban Mahmoud Abd El 한국독성학회 2024 Toxicological Research Vol.40 No.1
One of the commoly used chemotherapeutic agents is 5-Fluorouracil (5-FU). Unfortunately, the clinical administration of 5-FU is complicated with serious cardiotoxic effects and the safe use becomes an urgent task in cardio-oncology. Till now, there are no studies discussed the role of empagliflozin (EMP) against 5-FU cardiotoxicity. Thus, we investigated this effect and the involved mechanisms in 5-FU induced heart injury. Forty male rats of Wistar albino species were used and divided randomly into four groups. Group I is the control group, group II is EMP given group, group III is 5-FU cardiotoxic group and group IV is 5-FU plus EMP group. 5-FU (150 mg/kg) was administered as a single intraperitoneal (i.p.) dose on 1st day to induce cardiotoxicity with or without EMP (30 mg/kg/d) orally for 5 days. The dose of 5-FU is relevant to the human toxic dose. Our data showed that 5-FU given group caused cardiotoxicity with significant increase of serum cardiac enzymes, toll like receptors, enhancement of nuclear factor kappa B (NF-κB), interleukin1β (IL1β), IL6, myeloid-differentiation-factor 88 (MYD88), heart weight, malondialdehyde (MDA), tumor-necrosis-factor-alpha (TNFα), sodium glucose co-transporter 2 (SGLT2), P53 and caspase3 expression with clear histopathological features of cardiotoxicity. Moreover, there is a significant decrease in reduced glutathione (GSH) and total antioxidant capacity (TAC). Interestingly, co-administration of EMP could ameliorate 5-FU induced biochemical and histopathological changes. This effect may be due to modulation of SGLT2, decreasing inflammation, oxidative stress and apoptosis with downregulation of an essential inflammatory cascade that mediates 5-FU cardiotoxicity; TNFα/TLR/NF-κB.
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Mustafa, Ebtihal H,Mahmoud, Huda T,Al-Hudhud, Mariam Y,Abdalla, Maher Y,Ahmad, Iman M,Yasin, Salem R,Elkarmi, Ali Z,Tahtamouni, Lubna H Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.8
Background: Cancer metastasis depends on cell motility which is driven by cycles of actin polymerization and depolymerization. Reactive oxygen species (ROS) and metabolic oxidative stress have long been associated with cancer. ROS play a vital role in regulating actin dynamics that are sensitive to oxidative modification. The current work aimed at studying the effects of sub-lethal metabolic oxidative stress on actin cytoskeleton, focal adhesion and cell migration. Materials and Methods: T47D human breast cancer cells were treated with 2-deoxy-D-glucose (2DG), L-buthionine sulfoximine (BSO), or doxorubicin (DOX), individually or in combination, and changes in intracellular total glutathione and malondialdehyde (MDA) levels were measured. The expression of three major antioxidant enzymes was studied by immunoblotting, and cells were stained with fluorescent-phalloidin to evaluate changes in F-actin organization. In addition, cell adhesion and degradation ability were measured. Cell migration was studied using wound healing and transwell migration assays. Results: Our results show that treating T47D human breast cancer cells with drug combinations (2DG/BSO, 2DG/DOX, or BSO/DOX) decreased intracellular total glutathione and increased oxidized glutathione, lipid peroxidation, and cytotoxicity. In addition, the drug combinations caused a reduction in cell area and mitotic index, prophase arrest and a decreased ability to form invadopodia. The formation of F-actin aggregates was increased in treated T47D cells. Moreover, combination therapy reduced cell adhesion and the rate of cell migration. Conclusions: Our results suggest that exposure of T47D breast cancer cells to combination therapy reduces cell migration via effects on metabolic oxidative stress.
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Mudalal Mahmoud,Wang Zhanqi,Mustafa Shockry,Liu Yiping,Wang Yao,Yu Jize,Wang Shengnan,Sun Xiaolin,Zhou Yanmin 한국조직공학과 재생의학회 2021 조직공학과 재생의학 Vol.18 No.5
Background: An in vitro study on rapid culturing method of human gingival fibroblast cells (HGFCs) was established to investigate the potential use of the leukocyte-platelet rich fibrin (L-PRF) in tissue engineering technology, different medical fields, including periodontology and implantology. Methods: Eight biopsies were obtained from eight different donors and a modified culturing technique was developed to obtain HGFCs. The modified 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide MTT assay was used to compare the cell viability when the modified culturing method was used in comparison to the standard method. Blood samples were collected from the same patients and L-PRF was isolated using a standard protocol. The releases of platelet-derived growth factor-AA and transforming growth factor-beta1 at various time intervals were observed using enzyme-linked immunosorbent assay (ELISA) kit. The proliferative effect of L-PRF on HGFCs was assessed by the cell counting kit—8 assay. Results: A simple and rapid modified method for in vitro HGFC culture yielded a cellular monolayer within three to nine days after cell culture. L-PRF with three-dimensional polymer fibers released growth factors that peaked during the first three hours and continued to produce up to 10 days. The L-PRF presented a dose-dependent effect on HGFCs proliferation where HGFCs proliferation increased with an increase in L-PRF concentration. Conclusion: The modified technique for the culture of HGFCs might be useful for the development of future experimental and clinical studies, besides L-PRF has great therapeutic potential in oral surgery fields. Background: An in vitro study on rapid culturing method of human gingival fibroblast cells (HGFCs) was established to investigate the potential use of the leukocyte-platelet rich fibrin (L-PRF) in tissue engineering technology, different medical fields, including periodontology and implantology. Methods: Eight biopsies were obtained from eight different donors and a modified culturing technique was developed to obtain HGFCs. The modified 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide MTT assay was used to compare the cell viability when the modified culturing method was used in comparison to the standard method. Blood samples were collected from the same patients and L-PRF was isolated using a standard protocol. The releases of platelet-derived growth factor-AA and transforming growth factor-beta1 at various time intervals were observed using enzyme-linked immunosorbent assay (ELISA) kit. The proliferative effect of L-PRF on HGFCs was assessed by the cell counting kit—8 assay. Results: A simple and rapid modified method for in vitro HGFC culture yielded a cellular monolayer within three to nine days after cell culture. L-PRF with three-dimensional polymer fibers released growth factors that peaked during the first three hours and continued to produce up to 10 days. The L-PRF presented a dose-dependent effect on HGFCs proliferation where HGFCs proliferation increased with an increase in L-PRF concentration. Conclusion: The modified technique for the culture of HGFCs might be useful for the development of future experimental and clinical studies, besides L-PRF has great therapeutic potential in oral surgery fields.
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Lamis AbdelGadir Kaddam,Imad Fdl-Elmula,Omer Ali Eisawi,Haydar Awad Abdelrazig,Mustafa Khidir Elnimeiri,Amal Mahmoud Saeed 대한혈액학회 2019 Blood Research Vol.54 No.1
BackgroundSickle cell anemia (SCA) is a hereditary chronic hemolytic anemia with several clinical consequences. Intravascular sickling of red blood cells leads to multi-organ dysfunction. Moreover, several biochemical abnormalities have been associated with SCA. Gum arabic (GA) is an edible dried gummy exudate obtained from Acacia Senegal tree. GA showed antioxidant and cytoprotective activities and demonstrated protection against hepatic, renal, and cardiac toxicities in experimental rats. We hypothesized that regular intake of GA improves renal and liver functions in patients with SCA.MethodsForty-seven patients (5‒42 yr) carrying hemoglobin SS were recruited. The patients re-ceived 30 g/day GA for 12 weeks. Blood samples were collected before administering GA and then after 4, 8, and 12 weeks. Liver enzymes, total protein, albumin, electrolytes, urea, creatinine, and uric acid were determined in the serum. The study was approved by the Al Neelain University Institutional Review Board and Research Ethics Committee Ministry of Health. The trial was registered at ClinicalTrials.gov (identifier: NCT0246725).ResultsGA significantly decreased direct bilirubin level [statistical significance (P-value)=0.04]. It also significantly decreased serum alanine transaminase level after 4 weeks, which was sustained till the 8th week. GA, however, had no effect on serum aspartate transaminase level. In terms of renal function, GA decreased serum urea level but the effect was not sustained after the first month.ConclusionGA may alter the disease severity in SCA as demonstrated by its ability to decrease direct bilirubin and urea levels in the serum.
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Lamis AbdelGadir Kaddam,Imad Fdl-Elmula,Omer Ali Eisawi,Haydar Awad Abdelrazig,Mustafa Khidir Elnimeiri,Amal Mahmoud Saeed 대한혈액학회 2019 Blood Research Vol.54 No.1
BackgroundSickle cell anemia (SCA) is a hereditary chronic hemolytic anemia with several clinical consequences. Intravascular sickling of red blood cells leads to multi-organ dysfunction. Moreover, several biochemical abnormalities have been associated with SCA. Gum arabic (GA) is an edible dried gummy exudate obtained from Acacia Senegal tree. GA showed antioxidant and cytoprotective activities and demonstrated protection against hepatic, renal, and cardiac toxicities in experimental rats. We hypothesized that regular intake of GA improves renal and liver functions in patients with SCA.MethodsForty-seven patients (5‒42 yr) carrying hemoglobin SS were recruited. The patients re-ceived 30 g/day GA for 12 weeks. Blood samples were collected before administering GA and then after 4, 8, and 12 weeks. Liver enzymes, total protein, albumin, electrolytes, urea, creatinine, and uric acid were determined in the serum. The study was approved by the Al Neelain University Institutional Review Board and Research Ethics Committee Ministry of Health. The trial was registered at ClinicalTrials.gov (identifier: NCT0246725).ResultsGA significantly decreased direct bilirubin level [statistical significance (P-value)=0.04]. It also significantly decreased serum alanine transaminase level after 4 weeks, which was sustained till the 8th week. GA, however, had no effect on serum aspartate transaminase level. In terms of renal function, GA decreased serum urea level but the effect was not sustained after the first month.ConclusionGA may alter the disease severity in SCA as demonstrated by its ability to decrease direct bilirubin and urea levels in the serum.
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