http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Chen, Yong,Luo, Hailing,Liu, Xueliang,Wang, Zhenzhen,Zhang, Yuwei,Liu, Kun,Jiao, Lijuan,Chang, Yanfei,Zuo, Zhaoyun Asian Australasian Association of Animal Productio 2013 Animal Bioscience Vol.26 No.5
To investigate the effect of restricted grazing time on behavior of Tan sheep on desert steppe, forty 4-months old male Tan sheep with an original body weight (BW) of $15.62{\pm}0.33$ kg were randomly allocated to 4 grazing groups which corresponded to 4 different restricted grazing time treatments of 2 h/d (G2), 4 h/d (G4), 8 h/d (G8) and 12 h/d (G12) access to pasture. The restricted grazing times had a significant impact on intake time, resting time, ruminating time, bite rate and movement. As the grazing time decreased, the proportion of time spent on intake, bite rate and grazing velocity significantly (p<0.05) increased, but resting and ruminating time clearly (p<0.05) decreased. The grazing months mainly depicted effect on intake time and grazing velocity. In conclusion, by varying their foraging behavior, Tan sheep could improve grazing efficiency to adapt well to the time-limited grazing circumstance.
5-Formylhonokiol exerts anti-angiogenesis activity via inactivating the ERK signaling pathway
Wei Zhu,Lijuan Chen,Afu Fu,Jia Hu,Tianen Wang,Youfu Luo,Ming Peng,Yinghua Ma,Yuquan Wei 생화학분자생물학회 2011 Experimental and molecular medicine Vol.43 No.3
Our previous report has demonstrated that 5-formylhonokiol (FH), a derivative of honokiol (HK), exerts more potent anti-proliferative activities than honokiol in several tumor cell lines. In present study, we first explored the antiangiogenic activities of 5-formylhonokiol on proliferation, migration and tube formation of human umbilical vein endothelial cells (HUVECs) for the first time in vitro. Then we investigated the in vivo antiangiogenic effect of 5-formylhonokiol on zebrafish angiogenesis model. In order to clarify the underlying molecular mechanism of 5-formylhonokiol, we investigated the signaling pathway involved in controlling the angiogenesis process by western blotting assay. Wound-healing results showed that 5-formylhonokiol significantly and dose-dependently inhibited migration of cultured human umbilical vein enthothelial cells. The invasiveness of HUVEC cells was also effectively suppressed at a low concentration of 5-formylhonokiol in the transwell assay. Further F-actin imaging revealed that inhibitory effect of 5-formylhonokiol on invasion may partly contribute to the disruption of assembling stress fiber. Tube formation assay, which is associated with endothelial cells migration,further confirmed the anti-angiogenesis effect of 5-formylhonokiol. In in vivo zebrafish angiogenesis model, we found that 5-formylhonokiol dose-dependently inhibited angiogenesis. Furthermore, western blotting showed that 5-formylhonokiol significantly down-regulated extracellular signal-regulated kinase (ERK) expression and inhibited the phosphorylation of ERK but not affecting the total protein kinase B (Akt)expression and related phosphorylation, suggesting that 5-formylhonokiol might exert anti-angiogenesis capacity via down-regulation of the ERK signal pathway. Taken together, these data suggested that 5-formylhonokiol might be a viable drug candidate in antiangiogenesis and anticancer therapies.
Bokai Liao,Zhigang Luo,Shan Wan,Lijuan Chen 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.117 No.-
In this work, the extract of acanthopanax senticosus leaf (ASLE), prepared using one-step pure waterextraction method, is firstly utilized as a sustainable eco-friendly corrosion inhibitor for carbon steel inacidic solutions. The corrosion inhibition efficiency is evaluated by weight loss method and electrochemicaltests, including electrochemical impedance spectroscopy and potentiodynamic polarization curve. The compositions of ASLE are analyzed using Fourier transform infrared spectroscopy, components andmicrostructures of corrosion products are characterized by scanning electron microscopy and X-ray photoelectronspectroscopy. Results indicate that ASLE is successfully prepared using the clean productionprocess, and it can act as an efficient mixed-type corrosion inhibitor for Q235 carbon steel in 1 mol/LHCl. The maximum corrosion inhibition efficiency can reach 98.79 % after 144 h immersion with the additionof 150 mg/L. ASLE also displays the superior long-term corrosion inhibition performance. The protectivehydrophobic adsorption film can effectively retard the invasion of aggressive ions and inhibit thecorrosion of carbon steel in acidic service occasions.
5-Formylhonokiol exerts anti-angiogenesis activity $via$ inactivating the ERK signaling pathway
Zhu, Wei,Fu, Afu,Hu, Jia,Wang, Tianen,Luo, Youfu,Peng, Ming,Ma, Yinghua,Wei, Yuquan,Chen, Lijuan Korean Society for Biochemistry and Molecular Bion 2011 Experimental and molecular medicine Vol.43 No.3
Our previous report has demonstrated that 5-formylhonokiol (FH), a derivative of honokiol (HK), exerts more potent anti-proliferative activities than honokiol in several tumor cell lines. In present study, we first explored the antiangiogenic activities of 5-formylhonokiol on proliferation, migration and tube formation of human umbilical vein endothelial cells (HUVECs) for the first time $in$ $vitro$. Then we investigated the $in$ $vivo$ antiangiogenic effect of 5-formylhonokiol on zebrafish angiogenesis model. In order to clarify the underlying molecular mechanism of 5-formylhonokiol, we investigated the signaling pathway involved in controlling the angiogenesis process by western blotting assay. Wound-healing results showed that 5-formylhonokiol significantly and dose-dependently inhibited migration of cultured human umbilical vein enthothelial cells. The invasiveness of HUVEC cells was also effectively suppressed at a low concentration of 5-formylhonokiol in the transwell assay. Further F-actin imaging revealed that inhibitory effect of 5-formylhonokiol on invasion may partly contribute to the disruption of assembling stress fiber. Tube formation assay, which is associated with endothelial cells migration, further confirmed the anti-angiogenesis effect of 5-formylhonokiol. In $in$ $vivo$ zebrafish angiogenesis model, we found that 5-formylhonokiol dose-dependently inhibited angiogenesis. Furthermore, western blotting showed that 5-formylhonokiol significantly down-regulated extracellular signal-regulated kinase (ERK) expression and inhibited the phosphorylation of ERK but not affecting the total protein kinase B (Akt) expression and related phosphorylation, suggesting that 5-formylhonokiol might exert anti-angiogenesis capacity $via$ down-regulation of the ERK signal pathway. Taken together, these data suggested that 5-formylhonokiol might be a viable drug candidate in antiangiogenesis and anticancer therapies.
Wen Jing,Deng Chaonan,Shi Lixin,Zhou Shi,Zhang Miao,Hu Xiaoli,Wang Nianxue,Luo Lijuan 대한독성 유전단백체 학회 2022 Molecular & cellular toxicology Vol.18 No.2
Background Hashimoto's thyroiditis (HT), also known as chronic lymphocytic thyroiditis, is one of the most common autoimmune disease (AITD) in clinical practice. It is urgent to explore the mechanism of amiodarone-induced thyroid dysfunction. Objective This study aims to assess the expression levels of miR-214 and FasL in amiodarone contact type of HT, and the effect of miR-214 on cell viability and apoptosis and potential mechanism. Results We found that miR-214 was low expressed in the tissues of amiodarone-treated thyroiditis patients. MiR-214 increased the survival rate of amiodarone-induced thyroid epithelial follicular cells and inhibited apoptosis. Mechanically, we found that miR-214 could bind to FASL and regulate MAPK signaling pathway through FASL. Conclusions Our results suggested that miR-214 could be a potential therapeutic target for Hashimoto's thyroiditis.