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- 저자 : Luis E. Valencia (검색결과 2 건)
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Grafting improves salinity tolerance of bell pepper plants during greenhouse production
Blanca E. Orosco-Alcalá,Héctor G. Núñez-Palenius,Fidel Díaz-Serrano,Luis Pérez-Moreno,Mauricio Valencia-Posadas,Libia I. Trejo-Tellez,Nicacio Cruz-Huerta,Juan I. Valiente-Banuet 한국원예학회 2021 Horticulture, Environment, and Biotechnology Vol.62 No.6
Biotic and abiotic stresses aff ect plant growth and productivity. High-salinity stress aff ects crop yield, causing fi nancialloss to growers. The use of salt-tolerant rootstocks is a strategy that has been used to reduce salt damage in crops. A studywas conducted to evaluate the eff ect of salinity-resistant rootstocks on the physiological and morphological characteristicsof bell pepper plants grown under greenhouse conditions. A factorial experiment was conducted using a completely randomizeddesign with two factors. The bell pepper ‘Viper’ cultivar was grafted on two reported salinity-tolerant rootstocks(E21R10144 and E21R10197), and non-grafted (NG) plants were used as the control. Four salinity levels were applied tothe plants (electrical conductivity treatments using NaCl of 2, 4, 6, and 8 dS m −1 ) in Steiner nutritive solution (100%). Theresponse to salinity was determined using morphological and physiological plant parameters, including fruit yield. Increasedtolerance to salinity conditions (NaCl) was observed in the ‘Viper’ bell pepper grafted on E21R10144, which allowed greaterplant height, stem diameter, leaf size, as well as fresh and dry biomass of both the roots and canopy. The impacts on plantphysiological response, including photosynthesis, stomatal conductance, transpiration, water content, stomatal density, andfoliar area, were also determined. Our results indicate that the use of the salinity-tolerant bell pepper rootstock E21R10144maintained plant homeostasis and minimized the damage caused by salts to the morphology and physiology, as well as eff ectson fruit yield; thus, it is a promising tool for the management of salt stress.
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Bo Pang,Luis E. Valencia,Jessica Wang,Yao Wan,Ravi Lal,Amin Zargar,Jay D. Keasling 한국생물공학회 2019 Biotechnology and Bioprocess Engineering Vol.24 No.3
Modular type I polyketide synthases (PKSs) are multifunctional proteins that are comprised of individual domains organized into modules. These modules act together to assemble complex polyketides from acyl-CoA substrates in a linear fashion. This assembly-line enzymology makes engineered PKSs a potential retrobiosynthetic platform to produce fuels, commodity chemicals, speciality chemicals, and pharmaceuticals in various host microorganisms, including bacteria and fungi. However, the realization of this potential is restricted by practical difficulties in strain engineering, protein overexpression, and titer/yield optimization. These challenges are becoming more possible to overcome due to technical advances in PKS design, engineered heterologous hosts, DNA synthesis and assembly, PKS heterologous expression, and analytical methodology. In this review, we highlight these technical advances in PKS engineering and provide practical considerations thereof.
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