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Luan Shi,Zhuqing Liu,Wei Yang,Yaojun Li,Xiaodong Liu,Ran Tao 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.10
If a centrifugal impeller is in deep stall, it requires a large amount of additional energy to break free. As such, it is crucial to study the prediction method of impeller stall and establish its relationship with force and torque. This study employs a computational fluid dynamics numerical simulation method to analyze the flow field within the impeller of a centrifugal pump, while monitoring the changes in hydraulic torque of the blades. By examining the impeller’s internal flow field characteristics and external (energy performance) characteristics curves under different working conditions, the stall process was classified into three stages: germination, transition and stabilization. The evolution of stall cells under different stall stages was also analyzed. We utilized time-frequency conversion and analysis to extract characteristic parameters from instantaneous hydraulic torque results generated under different working conditions. A more effective model for predicting impeller stall stages was built by virtue of support vector machine algorithm. The proposed model exhibits improved feasibility and effectiveness in comparison to traditional prediction methods. It is expected to have significant implications for stable operation monitoring and safety protection of centrifugal pumps.
Sun Shi jun,Zhao Yun duo,Luan Ying Tao 국제온돌학회 2013 International Journal of Ondol Vol.2013 No.-
Straw board is renewable natural building material with the advantages of green environmental protection, strong applicability. But it hadn’t been promoted because of many reasons such as technology and economy. Through the analysis of the unique advantages and the recent new cases of straw board buildings in Harbin, and the current obstacles to application, The effective ways and the feasible solutions to promote sustainable straw board buildings are summarized.
Reuse of Spent FCC Catalyst for Removing Trace Olefins from Aromatics
Pu, Xin,Luan, Jin-Ning,Shi, Li Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.8
Pretreatment of spent FCC catalyst and its application in remove trace olefins in aromatics were investigated in this research. The most effective pretreatment route of spent FCC catalyst was calcining at $700^{\circ}C$ for 1 h, washing with 5% oxalic acid solution in ultrasonic reactor and dried. Treated spent FCC catalyst was modified with metal halides, then to prepare catalyst to remove trace olefins in aromatics. X-ray diffraction, Pyridine-FTIR, $N_2$ adsorption-desorption and inductively coupled plasma optical emission spectrometer (ICP-OES) were used to investigate the pretreatment process. The result showed that the performance of the treated spent FCC catalyst was much greater than that of the spent FCC catalyst, which indicted the possibility and improvement of this research.
Reuse of Spent FCC Catalyst for Removing Trace Olefins from Aromatics
Xin Pu,Jin-ning Luan,Li Shi 대한화학회 2012 Bulletin of the Korean Chemical Society Vol.33 No.8
Pretreatment of spent FCC catalyst and its application in remove trace olefins in aromatics were investigated in this research. The most effective pretreatment route of spent FCC catalyst was calcining at 700 oC for 1 h, washing with 5% oxalic acid solution in ultrasonic reactor and dried. Treated spent FCC catalyst was modified with metal halides, then to prepare catalyst to remove trace olefins in aromatics. X-ray diffraction, Pyridine- FTIR, N2 adsorption-desorption and inductively coupled plasma optical emission spectrometer (ICP-OES) were used to investigate the pretreatment process. The result showed that the performance of the treated spent FCC catalyst was much greater than that of the spent FCC catalyst, which indicted the possibility and improvement of this research.
Optimized Transformation of Streptomyces sp. ATCC 39366 Producing Leptomycin by Electroporation
Yong-Qiang Fan,Hong-Jian Liu,Li Yan,Yu-Shi Luan,Hai-Meng Zhou,Jun-Mo Yang,Shang-Jun Yin,Yu-Long Wang 한국미생물학회 2013 The journal of microbiology Vol.51 No.3
Streptomyces sp. ATCC 39366 produces leptomycin derivatives. Leptomycin B, a potent and specific inhibitor against the export of nuclear proteins, is the main product; however, the introduction of DNA into this strain is almost impossible, which has impeded its further use. We developed a Streptomyces sp. ATCC 39366 transformation protocol to introduce foreign DNA via electroporation. Various conditions were examined, including treatments of the cell wall with weakening agents, electroporation parameters, and DNA content. We found that only plasmid DNA isolated from a dam- ET12567 strain resulted in successful transformation. The mycelium growing in a yeast-peptone-dextrose medium supplemented with 1% glycine at 28°C on a rotary shaker (220 rpm) was more dispersed than those without supplementation and prone to electroporation. The maximum transformation efficiency of 8×102 CFU/μg plasmid DNA was obtained at a field strength of 13 kV/cm with a time constant of 13 ms (25-μF capacitor; parallel resistance, 600 Ω) using 1-mm electrocuvettes. The results of the transformations of two other Streptomyces species indicated that the optimized conditions established in this study might only be applicable to Streptomyces sp. ATCC 39366. However, this is the first report of successful transformation of Streptomyces sp. ATCC 39366, and will facilitate the construction of a gene knockout mutant in Streptomyces sp. ATCC 39366 to produce series of new leptomycin derivatives.
Fan, Yong-Qiang,Liu, Hong-Jian,Li, Chang,Luan, Yu-Shi,Yang, Jun-Mo,Wang, Yu-Long Humana Press 2013 Applied biochemistry and biotechnology Vol.169 No.1
<P>In this study, we quantitatively examined the effects of the macromolecular crowding agents, polyethylene glycol 2000 (PEG 2000) and dextran 70, on guanidine hydrochloride (GdnHCl)-induced denaturation of recombinant human brain-type creatine kinase (rHBCK). Our results showed that both PEG 2000 and dextran 70 had a protective effect on the inactivation of rHBCK induced by 0.5 M GdnHCl at 25 C. The presence of 200 g/L PEG 2000 resulted in the retention of 35.33 % of rHBCK activity after 4 h of inactivation, while no rHBCK activity was observed after denaturation in the absence of macromolecular crowding agents. The presence of PEG 2000 and dextran 70 at a concentration of 100 g/L could decelerate the k (2) value of the slow track to 21 and 33 %, respectively, in comparison to values obtained in the absence of crowding agents. Interestingly, inactivation of rHBCK in the presence of 200 g/L PEG 2000 followed first-order monophasic kinetics, with an apparent rate constant of 8??10(-5)?s(-1). The intrinsic fluorescence results showed that PEG 2000 was better than dextran 70 at stabilizing rHBCK conformation. In addition, the results of the phase diagram indicate that more intermediates may be captured when rHBCK is denatured in a macromolecular crowding system. Mixed crowding agents did not produce better results than single crowding agents, but the protective effects of PEG 2000 on the inactivation and unfolding of rHBCK tended to increase as the ratio of PEG 2000 increased in the mixed crowding agent solution. Though it is not clear which crowding agents more accurately simulated the intracellular environment, this study could lead to a better understanding of protein unfolding in the intracellular environment.</P>
Ji-Chang Zhou,Yu-Mei Zhu,Zheng Chen,Shan He,Shi-jie Zheng,Jun-luan Mo,Xiao-Li Liu,Chun-mei Gong,Bin Hou,Hui Yang 한국유전학회 2015 Genes & Genomics Vol.37 No.5
Two single nucleotide polymorphisms (SNPs), rs2280788 (-28C[G) and rs2107538 (-403G[A), in the promoter region of chemokine (C–C motif) ligand 5 (CCL5) was reported to be involved in the immunoglobulin E (IgE) expression and IgE-mediated allergic reactions. This study was to investigate the characteristics of total serum IgE level, specific allergen sensitivities and the two SNPs in the allergic skin disease (ASD) patients. ASD patients visiting the dermatological outpatient department of a local hospital were included with certain criteria, and the fasting venous blood was sampled for analysis. Total serum IgE was assayed with an ELISA kit, and 14 kinds of allergen-specific IgE were tested with an allergen screening system. The polymerase chain reaction–restriction fragment length polymorphism method was used to analyze the two SNPs. Among the finally included 437 patients aged from 16 to 85 years, 68.2 % was positive for the total serum IgE, 49.2 % was positive for at least one of the assayed allergen-specific IgE, and 35.0 % was sensitive to house dust mite. In the SNPs analysis, the GG/(GA?AA) ratio and G/A ratio for the -403G[A locus in the male and/or female C45 years subgroup were significantly lower in the total serum IgE positive patients than in the negative patients (P\0.05). Weak linkage disequilibrium was found between -403A and -28C alleles in male subgroups adjusted by age. Conclusively, house dust mite was the most common allergen in ASD patients, and -403A allele of CCL5 promoter was a risk factor for IgE-mediated sensitization.
Xiu Ying Xia,Na Xu,Da Ke Xu,Jun Yang,Yu Shi Luan 한국유전학회 2008 Genes & Genomics Vol.30 No.1
The genetic relationships among 17 clones of Vaccinium (Vaccinium spp.), including 2 Northeast Chinese wild species and 15 commercial cultivars were evaluated through a Random Amplified Polymorphic DNA(RAPD)analysis of young leaves. The Rapids generated a total of 115 markers with 15 random primers, of which 98.26% were found to be polymorphic. These RAPD markers could successfully separate individuals from the 17 samples and detect variations in the Vaccinium species. Genetic similarity and genetic distance were measured by Nei`s unbiased measurement. Using arithmetic averages (UPGMA) dendrogram, the unweighted pair-group method indicated that 17 samples were classified into three major groups: two groups of each wild species, and one group of 15 cultivars. The 15 cultivars could be clearly assigned into two subgroups of which the only lowbush sample (Blomidon) was clustered in one subgroup and the other 14 cultivars were clustered in one subgroup. The cluster results had some relativity with the pedigree of cultivars. These indicated that the polymorphic RAPD markers developed in this study should have general utility for identification of the varieties and examination of genetic relationships in blueberry.