Revelations of Lao Tzu’s Views on Human Nature to Counseling

Liu Sheng-ai 한국교육치료학회 2014 교육치료연구 Vol.6 No.1

상담은 인간을 다루는 분야로서 이 과정의 중심이 되는 인간 이해는 상담에 결정적인 영향을 미친다. 한 인간을 이해하려면 그가 속해 있는 문화권을 함께 이해해야 하고, 이런 인간을 다루고 있는 상담 역시 도움을 받고자 하는 내담자가 속해 있는 문화에 부합되어야 한다. 한국과 중국을 포함한 동양 사회를 살펴보면 수십세기 동안 주로 유교와 불교 그리고 노자 사상의 영향을 받아왔다. 이 중 노자의 철학은 중국 철학사상 최초로 완전한 철학 사상 이론을 정립한 사상으로서 동양 철학과 전통문화에 깊은 영향을 미쳤을 뿐만 아니라 사람들의 세계관, 사고방식, 행동양식 등에도 많은 영향을 미쳤다. 따라서 노자 철학 사상에 입각한 상담 이론의 고찰은 동양 사회의 부응하는 상담이론의 정립에 도움이 된다. Tao Te Ching, the Taoist classic written by Lao Tzu, not only expounds the way (Tao) of the universe, but also well presents Lao Tzu’s views on human nature, psychological problems and the causes, propositions of a mature person, etc. These profound thoughts can bring valuable revelations to counselors, especially those who serve in East Asia.

중국 절강성 항주시 행정대학 공공행정학부

유성애 ( Sheng Ai Liu ) 한국동서정신과학회 2015 동서정신과학 Vol.18 No.1

국문요약: 상담은 인간을 다루는 분야로서 상담의 중심에 서 있는 인간에 대한 이해가 상담의 성격을 결정한다 해도 과언이 아니다. 인간은 그가 처해 있는 사회와 문화의 영향을 받기 때문에 한 인간을 이해하려면 그가 속해 있는 문화권을 함께 이해해야 하고 이런 인간을 다루는 상담 역시 그 문화에 부합되어야 한다. 중국의 상담은 초창기(1949~1965), 침체기(1966~1977), 소생기(1978~1986), 상승기(1987~현재) 등 네 단계의 발전과정을 거치면서 현재까지 왔다. 이 과정에서 중국의 문화와 전통이 상담에 줄곧 영향을 끼쳐왔는데 그 영향은 주로 유가, 도가 등 전통사상의 영향, 중국이라는 특수한 사회체제의 영향이다. 이 영향으로 인해 중국의 상담은 심리치료와 상담의 미분화, 상담과 사상정치교육의 결부, 지시적 상담에 대한 선호 등 특징을 지니고 있다. 특히 중국이라는 특정사회에서 이 사회에 부합하는 본토화 상담이론을 정립하려는 노력이 상담의 발전 초기부터 시작되었다는 것이 중국 상담의 또 다른 특징이라 할 수 있겠다. 중국 본토화 상담의 연구는 주로 중국의 사회 문화에 부합하는 상담이론 연구, 중국 전통사상에 나타난 상담원리 연구 등 두가지 면에서 진행되어 왔으며 앞으로도 지속될 것이다. Like any other human activity, counseling is inevitably influenced by the socio-cultural context and such influence is substantial. Throughout the four periods that counseling has undergone in China . the beginning period (1949-1965), the recession period (1966-1977), the revival period (1978-1986), and the rising period (1987 to present) . the socio-cultural influence on counseling primarily from the traditional philosophies such as Confucianism and Taoism, and the current socio-cultural context, has never ceased. It is this unique influence that has made Chinese counseling characteristic of a mixture of counseling and psychotherapy, a combination of ideological and political education, and the preference of directive counseling. Meanwhile, another feature of Chinese counseling, emerging at the beginning period, is the localized study of counseling, which mainly focuses on the areas: developing counseling theory of Chinese characteristics based upon the Chinese socio-cultural context, and applying Chinese traditional thoughts into counseling practice.

Directed Evolution of Beta-galactosidase from Escherichia coil into Beta-glucuronidase

( Ai Sheng Xiong ),( Ri He Peng ),( Jing Zhuang ),( Jin Ge Liu ),( Fang Xu ),( Bin Cai ),( Zhao Kui Guo ),( Yu Shan Qiao ),( Jian Min Chen ),( Zhen Zhang ),( Quan Hong Yao ) 생화학분자생물학회 2007 BMB Reports Vol.40 No.3

Isolation and Characterization of a cDNA Encoding Two Novel Heat-shock Factor OsHSF6 and OsHSF12 in Oryza Sativa L.

Liu, Jin-Ge,Yao, Quan-Hong,Zhang, Zhen,Peng, Ri-He,Xiong, Ai-Sheng,Xu, Fang,Zhu, Hong Korean Society for Biochemistry and Molecular Biol 2005 Journal of biochemistry and molecular biology Vol.38 No.5

As a crucial transcription factor family, heat-shock factors were mainly analyzed and characterized in tomato and Arabidopsis. In this study, we isolated two putative heat shock factors OsHSF6 and OsHSF12 that interact specifically with heat-shock element (HSE) from Oryza sativa L by yeast one-hybrid method. The full-length cDNA of OsHSF6 and OsHSF12 have 1074bp and 920bp open reading frame (ORF), respectively. Analysis of the deduced amino acid sequences revealed that OsHSF6 was a class A heat shock factor (HSF) with all the conserved sequence elements characteristic of heat stress transcription factor, while OsHSF12 was a class B HSF with C-terminal domain (CTD) lacking of AHA motif. Bioinformatic analysis showed that the sequences and structures of two HSFs' DNA binding domain (DBD) had a high similarity with LpHSF24. The results of RT-PCR indicated OsHSF6 gene was expressed immediately after rice plants exposure to heat stress, and the transcription of OsHSF6 gene accumulated primarily in immature seeds, roots and leaves. However, we did not find the transcription of OsHSF12 gene in different organs and growth periods. Our results implied that OsHSF6 might be function as a HSF regulating early expression of stress genes in response to heat shock, and OsHSF12 might be act as a synergistic factor to regulate the expression of down-stream genes.

Directed Evolution of Beta-galactosidase from Escherichia coli into Beta-glucuronidase

Xiong, Ai-Sheng,Peng, Ri-He,Zhuang, Jing,Liu, Jin-Ge,Xu, Fang,Cai, Bin,Guo, Zhao-Kui,Qiao, Yu-Shan,Chen, Jian-Min,Zhang, Zhen,Yao, Quan-Hong Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.3

In vitro directed evolution through DNA shuffling is a powerful molecular tool for creation of new biological phenotypes. E. coli $\beta$-galactosidase and $\beta$-glucuronidase are widely used, and their biological function, catalytic mechanism, and molecular structures are well characterized. We applied an in vitro directed evolution strategy through DNA shuffling and obtained five mutants named YG6764, YG6768, YG6769, YG6770 and YG6771 after two rounds of DNA shuffling and screening, which exhibited more $\beta$-glucuronidase activity than wild-type $\beta$-galactosidase. These variants had mutations at fourteen nucleic acid sites, resulting in changes in ten amino acids: S193N, T266A, Q267R, V411A, D448G, G466A, L527I, M543I, Q626R and Q951R. We expressed and purified those mutant proteins. Compared to the wild-type protein, five mutant proteins exhibited high $\beta$-glucuronidase activity. The comparison of molecular models of the mutated and wildtype enzymes revealed the relationship between protein function and structural modification.

TGF-β1 Protein Expression in Non-Small Cell Lung Cancers is Correlated with Prognosis

Huang, Ai-Li,Liu, Shu-Guang,Qi, Wen-Juan,Zhao, Yun-Fei,Li, Yu-Mei,Lei, Bin,Sheng, Wen-Jie,Shen, Hong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.19

To investigate the expression intensity and prognostic significance of TGF-${\beta}1$ protein in non-small cell lung cancer (NSCLC), immunohistochemistry was carried out in 194 cases of NSCLC and 24 cases of normal lung tissues by SP methods. The PU (positive unit) value was used to assess the TGF-${\beta}1$ protein expression in systematically selected fields under the microscope with Leica Q500MC image analysis. We found that the TGF-${\beta}1$ PU value was nearly two-fold higher in NSCLC than in normal lung tissues (p=0.000), being associated with TNM stages (p=0.000) and lymph node metastases (p=0.000), but not to patient age, gender, smoking history, tumor differentiation, histological subtype and tumor location (P>0.05). Univariate analysis indicated that patients with high TGF-${\beta}1$ protein expression and lymph node metastases demonstrated a poor prognosis (both p=0.000,). Multivariate analysis showed that TGF-${\beta}1$ protein expression (RR = 2.565, p=0.002) and lymph node metastases (RR=1.874, p=0.030) were also independent prognostic factors. Thus, TGF-${\beta}1$ protein expression may be correlated to oncogenesis and serve as an independent prognostic biomarker for NSCLC.

Association of Lipoprotein-Associated Phospholipase A_2 with Characteristics of Vulnerable Coronary Atherosclerotic Plaques

Yu-Sheng Liu,Qing-Hua Lu,Xiao-Bo Hu,Hong-Zhuan Li,Wei-Dong Jiang,Xin Wang,Hao Lin,Ai-Qiong Qin,Yong-Mei Wang,Tong Zhao,Zhao-Qiang Dong,Mei Zhang 연세대학교의과대학 2011 Yonsei medical journal Vol.52 No.6

Purpose: Lipoprotein-associated phospholipase A2 (Lp-PLA_2) is an inflammatory enzyme expressed in atherosclerotic plaques. We investigated the association of circulating Lp-PLA_2 with characteristics of vulnerable coronary atherosclerotic plaques. Materials and Methods: We recruited 113 patients with either unstable angina (UA, n=59) and stable angina (SA, n=54) by coronary angiography. Thirty-six healthy subjects served as controls. Intravascular ultrasound (IVUS) was used to evaluate the characteristics of coronary atherosclerotic plaque, and serum Lp-PLA2 concentration was measured as well. Results: Lp-PLA_2 concentration was significantly higher in both UA and SA patients [(396±36) μg/L and (321±39) μg/L, respectively]compared with the controls [(127±49) μg/L, p<0.01], and higher in UA than SA group. IVUS findings showed that remodeling index (RI) (0.91±0.15 vs. 0.85±0.11, p=0.005) and eccentricity index (EI) (0.73±0.16 vs. 0.65±0.22, p=0.039) were larger in UA than in SA group, and fibrous caps were thicker in SA than UA group [(0.91±0.23) mm vs. (0.63±0.21) mm, p=0.032]. Moreover, Lp-PLA_2 correlated positively with EI (r=0.439, p<0.01) and RI (r=0.592, p<0.05) in UA group. There was an inverse relationship between Lp-PLA_2 and fibrous cap thickness in both UA (r=-0.587, p<0.001) and SA (r=-0.318, p<0.05) groups. The independent risk factors in UA group were Lp-PLA_2 (OR=1.055, 95% CI: 1.03-1.08, p=0.013), LDL-cholesterol (OR=0.032, 95% CI: 0.00-0.05, p=0.041) and fibrous cap thickness (OR=0.008, 95% CI: 0.00-0.45, p=0.019). Lp-PLA_2 was strongly associated with both EI and fibrous cap thickness in both groups. Conclusion: Serum level of Lp-PLA_2 is associated with both eccentricity index and fibrous cap thickness in both UA and SA groups. Elevated levels of circulating Lp-PLA_2 might to be a strong risk factor and more serious for unstable angina than stable angina.

Anti-Proliferation Effects and Molecular Mechanisms of Action of Tetramethypyrazine on Human SGC-7901 Gastric Carcinoma Cells

Ji, Ai-Jun,Liu, Sheng-Lin,Ju, Wen-Zheng,Huang, Xin-En Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.8

Aim: To investigate the effects of tetramethypyrazine (TMP) on proliferation and apoptosis of the human gastric carcinoma cell line 7901 and its possible mechanism of action. Methods: The viability of TMP-treated 7901 cells was measured with a 3-(4, 5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide assay (MTT) and cell apoptosis was analyzed by flow cytometry. The distribution of cells in different phases of cell cycle after exposure of TMPs was analyzed with flow cytometry. To investigate the molecular mechanisms of TMP-mediated apoptosis, the expression of NF-${\kappa}Bp65$, cyclinD1 and p16 in SGC-7901 cells was analyzed by reverse transcription-polymerase chain reaction (RT-PCR) and western blotting. Results: TMP inhibited the proliferation of human gastric carcinoma cell line 7901 in dose and time dependent manners. Cell growth was suppressed by TMP at different concentrations (0.25, 0.5, 1.0, 2.0 mg/ml), the inhibition rate is 0.46%, 4.36%, 14.8%, 76.1% (48h) and 15.5%, 18.5%, 41.2%, 89.8% (72h) respectively. When the concentration of TMPs was 2.0mg/ml, G1-phase arrest in the SGC-7901 cells was significant based on the data for cell cycle distribution. RT-PCR demonstrated that NF-${\kappa}Bp65$ and cyclin D1 mRNA expression was significantly down-regulated in 7901 cells treated with 2.0 mg/ml TMP for 72h (p<0.05), while the p16 mRNA level was up-regulated (p<0.05). The protein expression of NF-${\kappa}Bp65$ and cyclin D1 decreased gradually with the increase in TMP concentration, compared with control cells (p<0.05), while expression of protein p16 was up-regulated (p<0.01). Conclusion: TMP exhibits significant anti-proliferative and pro-apoptotic effects on the human gastric carcinoma cell line SGC-7901. NF-${\kappa}Bp65$, cyclinD1 and p16 may also play important roles in the regulation mechanisms.

Low-dose Radiation Induces Antitumor Effects and Erythrocyte System Hormesis

Yu, Hong-Sheng,Liu, Zi-Min,Yu, Xiao-Yun,Song, Ai-Qin,Liu, Ning,Wang, Hao Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.7

Objective: Low dose radiation may stimulate the growth and development of animals, increase life span, enhance fertility, and downgrade the incidence of tumor occurrence.The aim of this study was to investigate the antitumor effect and hormesis in an erythrocyte system induced by low-dose radiation. Methods: Kunming strain male mice were subcutaneously implanted with S180 sarcoma cells in the right inguen as an experimental in situ animal model. Six hours before implantation, the mice were given 75mGy whole body X-ray radiation. Tumor growth was observed 5 days later, and the tumor volume was calculated every other day. Fifteen days later, all mice were killed to measure the tumor weight, and to observe necrotic areas and tumor-infiltration-lymphoreticular cells (TILs). At the same time, erythrocyte immune function and the level of 2,3-diphosphoglyceric acid (2,3-DPG) were determined. Immunohistochemical staining was used to detect the expression of EPO and VEGFR of tumor tissues. Results: The mice pre-exposed to low dose radiation had a lower tumor formation rate than those without low dose radiation (P < 0.05). The tumor growth slowed down significantly in mice pre-exposed to low dose radiation; the average tumor weight in mice pre-exposed to low dose radiation was lighter too (P < 0.05). The tumor necrosis areas were larger and TILs were more in the radiation group than those of the group without radiation. The erythrocyte immune function, the level of 2,3-DPG in the low dose radiation group were higher than those of the group without radiation (P < 0.05). After irradiation the expression of EPO of tumor tissues in LDR group decreased with time. LDR-24h, LDR-48h and LDR-72h groups were all statistically significantly different from sham-irradiation group. The expression of VEGFR also decreased, and LDR-24h group was the lowest (P < 0.05). Conclusion: Low dose radiation could markedly increase the anti-tumor ability of the organism and improve the erythrocyte immune function and the ability of carrying $O_2$. Low-dose total body irradiation, within a certain period of time, can decrease the expression of hypoxia factor EPO and VEGFR, which may improve the situation of tumor hypoxia and radiosensitivity of tumor itself.

Concurrent Chemoradiotherapy Versus Radiotherapy Alone for Locoregionally Advanced Nasopharyngeal Carcinoma

Yu, Hong-Sheng,Wang, Xin,Song, Ai-Qin,Liu, Ning,Zhang, Wei,Yu, Li Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8

Objective: To compare the clinical effects of concurrent radiochemotherapy with those of radiotherapy in treating locally advanced nasopharyngeal carcinoma (Stage III~IVa). Methods: A total of 95 patients suffering from nasopharyngeal carcinoma (Stage III~IVa) were divided into two groups: concurrent radiochemotherapy (Group CCRT, n=49) and radiotherapy (Group RT, n=46). The two groups were both delivered conventional fractionated radiotherapy, while Group CCRT also received three cycles of PF (DDP+5-Fu) or PLF (DDP+5-Fu+CF) chemotherapy. Results: The complete remission rate and total remission rate of Group CCRT were higher than those of Group RT ($X^2$=4.72~7.19, P<0.05). The one-year overall survival (OS) rate calculated by the life table method, was also higher than that of Group RT ($X^2$=4.24, P<0.05) as well as the 3-year OS rate, nasopharyngeal control rate and cervical lymph nodes' control rate ($X^2$=4.28~4.40, P<0.05). In addition, the 5-year OS and metastasis-free rates of Group CCRT were higher than those of Group RT and the differences were of statistical importance ($X^2$=3.96~8.26, P<0.05). However, acute toxicity was also obviously higher, the difference in gastrointestinal reactions being statistically significant ($X^2$=11.70, P<0.05). Conclusion: This study demonstrated that concurrent radiochemotherapy could improve the remission rate, overall survival rate and locally control rate. The toxicity of concurrent radiochemotherapy could be tolerated by the patients.