Paclitaxel inhibits proliferation and promotes apoptosis through regulation ROS and endoplasmic reticulum stress in osteosarcoma cell

Li Ming,Yin Liming,Wu Lili,Zhu Yunsen,Wang Xi 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.4

Background Paclitaxel is a chemotherapeutic drug for cancer, which is isolated from the Pacifc yew tree. However, and the molecular mechanism and the antitumor efects of paclitaxel on osteosarcoma cell remain to be explored. Objective The aim of our study was to explore the possible molecular mechanisms of apoptosis in osteosarcoma induced by paclitaxel. Results Paclitaxel can obviously decrease the proliferation of HOS-732 cells in a dose-dependent manner. Paclitaxel could induce the cell cycle arrest at the G2/M-phase and decreases the CDK5 and CCNE1 expression in HOS-732 cells. Paclitaxel promotes cell apoptosis in HOS-732 cells, which may be contacted to the decreasing of Bcl-2 protein expression. Further, the production of ROS in HOS-732 cells was remarkably increased with the increasing concentration of paclitaxel. Moreover, paclitaxel induces the ER-stress related gene and protein expression (GRP79, DDIT3 mRNA and GRP78, XBP-1 s, IRE1α protein expression) in osteosarcoma cells. Conclusion Paclitaxel can inhibit the proliferation of HOS-732 cells and increase ROS and ER-stress response to promote cell apoptosis, suggesting that paclitaxel may represent a new therapeutic option for the treatment and prevention osteosarcoma.

Polymorphisms of Inflammatory Cytokine Genes and Risk for Intracranial Aneurysm: A Systematic Review and Meta-Analysis

Liming Hu,Bingyang Li,Xin Liao,Junxia Yan 연세대학교의과대학 2020 Yonsei medical journal Vol.61 No.5

Purpose: Inflammatory cytokines are thought to be involved in the pathogenesis of intracranial aneurysm (IA), although results among studies in the literature are inconsistent. This article sought to review studies on the associations among polymorphisms in inflammatory cytokine genes and IA risk and to provide recommendations for future research. Materials and Methods: A systematic search of PubMed, Embase, and Web of Science was conducted up to August 4, 2019. The associations between polymorphisms of inflammatory cytokine genes and IA risk were estimated by pooled odds ratios (ORs) and 95% confidence intervals (CIs). Subgroup analyses were performed according to race. Qualitative systematic review was conducted for variants that were studied in only one study. All analyses were performed using STATA 12.0. Results: 13 studies investigating the associations between polymorphisms in five inflammatory cytokine genes (TNF-α, IL-1α, IL-1β, IL6, and IL-12B) and IA were reviewed. Combined results showed that the A allele of TNF-α rs1800629 polymorphism has a protective effect against IA (dominant model: OR=0.65, 95% CI=0.47–0.89, p=0.007). No associations were identified between polymorphisms in IL-1α rs1800587, IL-1β rs16944, IL6 rs1800795 and rs1800796, or IL-12B rs3212227 and IA risk. Conclusion: This review demonstrated an association between TNF-α rs1800629 polymorphism and IA in Caucasians, illustrating the potentially important role of genes involved in inflammation in IA.

Enhanced ketalization activity of cyclohexanone and ethanediol over immobilized ionic liquid in mesoporous materials

Liming Dai,Qian Zhao,Haibo Zhao,Yingying Li,Tingshun Jiang 한국화학공학회 2017 Korean Journal of Chemical Engineering Vol.34 No.5

Three different mesoporous molecular sieves, including MCM-41, MCM-48, and SBA-15, were synthesized by hydrothermal process and characterized by XRD, BET and TEM. The chlorine-aluminate acidic ionic liquid of 1-butyl-3-methylimidazolium chloride-aluminum chloride ([Bmim]Cl-AlCl3, denoted as Al-ILs) was prepared by twostep method. The immobilized ionic liquid (SBA-15/Al-ILs, MCM-41/Al-ILs, MCM-48/Al-ILs) was prepared through impregnating Al-ILs. The structures of composite catalysts were characterized by XRD, BET, FT-IR, TEM and XPS. The amounts of aluminum present in the resulting composite catalysts were detected by ICP-AES to calculate the amount of AL-ILs impregnated. The ketalization between cyclohexanone and ethanediol was used as the model reaction to test the catalytic activities of the composite catalysts. The effects of molar ratio of the reactants, reaction time, the catalyst dose, as well as the addition of cyclohexane were discussed in detail. Also, catalytic activities of three catalysts with different pore sizes were compared. Under comparable conditions, the SBA-15/Al-ILs composite catalyst exhibited much high catalytic activity and gave a maximum yield that was ca. 85.1%.

Metal-Free Photocatalysts C3N3S3 and its Polymers: Solubility in Water and Application in Benzylic Alcohols Oxidation Under Visible Light

Liming Chen,Lulu Zhao,Jie Xu,Jinlin Long,Linxi Hou 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2017 NANO Vol.12 No.8

Metal-free photocatalysts trithiocyanuric acid (C3N3S3) and its polymers were one-step synthesized and used for selective aerobic oxidation of benzyl alcohol under visible light. The S–S bridged C3N3S3 polymers showed higher oxidation activity with increasing polymerization time and reached a maximum at 40 h. As a major factor, catalysts' solubility in water at different temperatures (from 298.15K to 368.15 K) was measured at atmospheric pressure. The obtained data were correlated by polynomial empirical equation and modified Apelblat equation, respectively. Experimental results proved that the solubility of C3N3S3 and its polymers increased with increasing temperature. The solubility of C3N3S3 polymers with lower polymerization degree (DP) were better than that of parent C3N3S3 owing to the hydrophilicity caused by a low symmetry and disorder structure, whereas the higher DP could lead to the decrease of polymer solubility and further affect the photocatalytic activity. Thermodynamic properties of the solution system including the enthalpy (△H0S) and heat capacity (c0p) were calculated by the modified Apelblat equation.

Noun Phrase Complexity in EFL Academic Writing : A Corpus-based Study of Postgraduate Academic Writing

Liming Liu,Lan Li 아시아영어교육학회 2016 The Journal of Asia TEFL Vol.13 No.1

Noun phrase (NP) centered structures are distinctive syntactic devices in academic discourse. The commonly employed subordination-based complexity measures cannot adequately capture the development of syntactic complexity of noun phrases expected of advanced student academic writing (Biber, Gray, & Poonpon, 2011). Following the call for more research in this area (e.g. Lu, 2011, p. 57), the current study compared noun phrase complexity in two corpora: one is a corpus of MA dissertations written by Chinese EFL students and the other comprises published research articles in applied linguistics journals. The study examined overall noun phrase complexity using an automatic syntactic complexity analyzer and specifically identified features of one aspect of NP complexity: NP postmodification. The quantitative results were further contextualized in a textual analysis of excerpts from the two corpora for demonstrating the significance of NP complexity to the establishment of discourse coherence. Results of the analyses showed significant underdevelopment of NP postmodification complexity in student writing relative to published texts, meanwhile explicating the circumstances under which the difference is meaningful. Implications of the findings for the teaching of EFL academic writing were also discussed.

Output Feedback Tracking Control of Flat Systems via Exact Feedforward Linearization and LPV Techniques

Liming Chen,Yingmin Jia 제어·로봇·시스템학회 2019 International Journal of Control, Automation, and Vol.17 No.3

In this article, an output feedback control scheme is proposed for trajectory tracking of differentiallyflat systems. After applying the exact feedforward linearization, the tracking error dynamics is linearized as alinear time-varying (LTV) system, where only the state matrix is time-varying while the input and output matricesare time-invariant. Using linear parameter-varying (LPV) techniques based on polytopes, for the LTV system thecontroller matrices, which are set to be affine on time-varying parameters, are calculated by solving a set of linearmatrix inequalities (LMIs). An example for trajectory tracking of a two-wheeled mobile robot is given to show theeffectiveness of the proposed method.

Map Scale Effects on Soil Phosphorus Storage Estimation in the Uplands of Eastern China

Liming Zhang,Jiajia Li,Dongsheng Yu,Xuezheng Shi,Shihe Xing,Shengxiang Xu,Yongcun Zhao,Fengyun Zhang 한국토양비료학회 2014 한국토양비료학회 학술발표회 초록집 Vol.2014 No.6

The Role of L1 in the Acquisition of Motion Verbs in English by Chinese and Japanese Learners

Liming Yu UNIV TORONTO PRESS 1996 CANADIAN MODERN LANGUAGE REVIEW Vol.35 No.1

Privacy-Preserving, Energy-Saving Data Aggregation Scheme in Wireless Sensor Networks

Liming Zhou,Yingzi Shan 한국정보처리학회 2020 Journal of information processing systems Vol.16 No.1

Because sensor nodes have limited resources in wireless sensor networks, data aggregation can efficientlyreduce communication overhead and extend the network lifetime. Although many existing methods areparticularly useful for data aggregation applications, they incur unbalanced communication cost and waste lotsof sensors’ energy. In this paper, we propose a privacy-preserving, energy-saving data aggregation scheme(EBPP). Our method can efficiently reduce the communication cost and provide privacy preservation to protectuseful information. Meanwhile, the balanced energy of the nodes can extend the network lifetime in our scheme. Through many simulation experiments, we use several performance criteria to evaluate the method. Accordingto the simulation and analysis results, this method can more effectively balance energy dissipation and provideprivacy preservation compared to the existing schemes.

A Parametric and Accurate CAD Model of Flat End Mills Based on Its Grinding Operations

Liming Wang,Lin Kong,Jian-Feng Li,Zezhong Chen 한국정밀공학회 2017 International Journal of Precision Engineering and Vol.18 No.10

End mills are widely used in CNC machining. However, the complex structures of end mills make it difficult to define the CAD model which determines the quality and performance of virtual cutting tests. This paper presents an accurate CAD model of end mill through analyzing kinematic models of grinding processes. Initially, a common parametric representation of grinding wheel is given. Base on the given wheel profile and the design parameters of cutters including the rake angle, core diameters and pitch angle, the representation of helix flute surface is first calculated using contact theory and also the formulation of rake angle and pitch angle are investigated. Next, four design parameters are used to control the shape of the gash which is formed via Boolean operation between the given wheel and end mill. Similarly, the peripheral edges and end edges are swept using grinding simulation. Finally, integrating all the above processes, a parametric CAD model of end mill is represented. The simulation results showed that the proposed CAD model could achieve 1e-3 mm and 2e-2 deg. in accuracy, which can be used to evaluate the grinding process or improve the future FEA analysis of end-mills.