Hepatoprotective effect of phenylethanoid glycosides from Incarvillea compacta against CCl4-induced cytotoxicity in HepG2 cells

Ting Shen,Xueqin Li,Weicheng Hu,Lijin Zhang,Xudong Xu,Haifeng Wu,Lilian Ji 한국응용생명화학회 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.4

The extraction and solvent partition of roots of Incarvillea compacta, a traditional Tibetan folk medicine, and repeated column chromatography and preparative high-performance liquid chromatography for n-butanol fraction yielded four phenylethanoid glycosides, crenatoside (1), 3000-O-methylcrenatoside (2), leucoseceptoside A (3), and martynoside (4). The chemical structures were identified on the basis of spectroscopic data analyses including NMR and MS. All compounds were isolated for the first time from the plant. Compound 1 exerted better 1,1-diphenyl-2-picrylhydrazyl free radical scavenging activity. In addition, compounds 1–4 were evaluated for their hepatoprotective activity on carbon tetrachloride (CCl4)- induced liver injury in HepG2 cells. Pretreatment of HepG2 cells with compound 1–4 significantly increased the viability on CCl4-induced cell death. Furthermore, compounds 1–4 also alleviated CCl4-induced hepatotoxicity by enhancement of the antioxidant enzyme activities of superoxide dismutase and reduction of the malondialdehyde content, intracellular ROS as well as NF-jB transactivation. Our results suggest that phenylethanoid glycosides ameliorate CCl4-induced cell injury, and this protection was likely due to antioxidative activity and down-regulation of NF-jB.

Hepatoprotective effect of phenylethanoid glycosides from Incarvillea compacta against CCl₄-induced cytotoxicity in HepG2 cells

Shen, Ting,Li, Xueqin,Hu, Weicheng,Zhang, Lijin,Xu, Xudong,Wu, Haifeng,Ji, Lilian 한국응용생명화학회 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.4

The extraction and solvent partition of roots of Incarvillea compacta, a traditional Tibetan folk medicine, and repeated column chromatography and preparative high-performance liquid chromatography for n-butanol fraction yielded four phenylethanoid glycosides, crenatoside (1), 3'''-O-methylcrenatoside (2), leucoseceptoside A (3), and martynoside (4). The chemical structures were identified on the basis of spectroscopic data analyses including NMR and MS. All compounds were isolated for the first time from the plant. Compound 1 exerted better 1,1-diphenyl-2-picrylhydrazyl free radical scavenging activity. In addition, compounds 1-4 were evaluated for their hepatoprotective activity on carbon tetrachloride ($CCl_4$)-induced liver injury in HepG2 cells. Pretreatment of HepG2 cells with compound 1-4 significantly increased the viability on $CCl_4$-induced cell death. Furthermore, compounds 1-4 also alleviated $CCl_4$-induced hepatotoxicity by enhancement of the antioxidant enzyme activities of superoxide dismutase and reduction of the malondialdehyde content, intracellular ROS as well as NF-${\kappa}B$ transactivation. Our results suggest that phenylethanoid glycosides ameliorate $CCl_4$-induced cell injury, and this protection was likely due to antioxidative activity and down-regulation of NF-${\kappa}B$.

Two new triterpenoid saponins derived from the leaves of Panax ginseng and their antiinflammatory activity

Li, Fu,Cao, Yufeng,Luo, Yanyan,Liu, Tingwu,Yan, Guilong,Chen, Liang,Ji, Lilian,Wang, Lun,Chen, Bin,Yaseen, Aftab,Khan, Ashfaq A.,Zhang, Guolin,Jiang, Yunyao,Liu, Jianxun,Wang, Gongcheng,Wang, Ming-Kui The Korean Society of Ginseng 2019 Journal of Ginseng Research Vol.43 No.4

Background: The leaves and roots of Panax ginseng are rich in ginsenosides. However, the chemical compositions of the leaves and roots of P. ginseng differ, resulting in different medicinal functions. In recent years, the aerial parts of members of the Panax genus have received great attention from natural product chemists as producers of bioactive ginsenosides. The aim of this study was the isolation and structural elucidation of novel, minor ginsenosides in the leaves of P. ginseng and evaluation of their antiinflammatory activity in vitro. Methods: Various chromatographic techniques were applied to obtain pure individual compounds, and their structures were determined by nuclear magnetic resonance and high-resolution mass spectrometry, as well as chemical methods. The antiinflammatory effect of the new compounds was evaluated on lipopolysaccharide-stimulated RAW 264.7 cells. Results and conclusions: Two novel, minor triterpenoid saponins, ginsenoside $LS_1$ (1) and 5,6-didehydroginsenoside $Rg_3$ (2), were isolated from the leaves of P. ginseng. The isolated compounds 1 and 2 were assayed for their inhibitory effect on nitric oxide production in LPS-stimulated RAW 264.7 cells, and Compound 2 showed a significant inhibitory effect with $IC_{50}$ of $37.38{\mu}M$ compared with that of NG-monomethyl-L-arginine ($IC_{50}=90.76{\mu}M$). Moreover, Compound 2 significantly decreased secretion of cytokines such as prostaglandin $E_2$ and tumor necrosis factor-${\alpha}$. In addition, Compound 2 significantly suppressed protein expression of inducible nitric oxide synthase and cyclooxygenase-2. These results suggested that Compound 2 could be used as a valuable candidate for medicinal use or functional food, and the mechanism is warranted for further exploration.

Two new triterpenoid saponins derived from the leaves of Panax ginseng and their antiinflammatory activity

Fuli Li,Yufeng Cao,Tingwu Liu,Guilong Yan,Liang Chen,Lilian Ji,Lun Wang,Bin Chen,Aftab Yaseen,Ashfaq A. Khan,Guo-Lin Zhang,Yunyao Jiang,Jianxun Liu,Gongcheng Wang,Ming-Kui Wang,Weicheng Hu 고려인삼학회 2019 Journal of Ginseng Research Vol.43 No.4

Background: The leaves and roots of Panax ginseng are rich in ginsenosides. However, the chemical compositionsof the leaves and roots of P. ginseng differ, resulting in different medicinal functions. In recent years,the aerial parts of members of the Panax genus have received great attention fromnatural product chemistsas producers of bioactive ginsenosides. The aim of this study was the isolation and structural elucidation ofnovel, minor ginsenosides in the leaves of P. ginseng and evaluation of their antiinflammatory activity in vitro. Methods: Various chromatographic techniques were applied to obtain pure individual compounds, andtheir structures were determined by nuclear magnetic resonance and high-resolution mass spectrometry,as well as chemical methods. The antiinflammatory effect of the new compounds was evaluated onlipopolysaccharide-stimulated RAW 264.7 cells. Results and conclusions: Two novel, minor triterpenoid saponins, ginsenoside LS1 (1) and 5,6-didehydroginsenoside Rg3 (2), were isolated from the leaves of P. ginseng. The isolated compounds 1and 2 were assayed for their inhibitory effect on nitric oxide production in LPS-stimulated RAW 264.7cells, and Compound 2 showed a significant inhibitory effect with IC50 of 37.38 mM compared with that ofNG-monomethyl-L-arginine (IC50 ¼ 90.76 mM). Moreover, Compound 2 significantly decreased secretionof cytokines such as prostaglandin E2 and tumor necrosis factor-a. In addition, Compound 2 significantlysuppressed protein expression of inducible nitric oxide synthase and cyclooxygenase-2. These resultssuggested that Compound 2 could be used as a valuable candidate for medicinal use or functional food,and the mechanism is warranted for further exploration.

Chemical Composition, Antioxidant and Cytoprotective Activities of Lotus Receptacle

Weicheng Hu,Gongcheng Wang,Ting Shen,Yuning Wang,Boran Hu,Xinfeng Wang,Lei Wu,Pengxia Li,Lilian Ji 한국원예학회 2015 Horticulture, Environment, and Biotechnology Vol.56 No.5

The nutritional composition (ash, protein, fat, crude fiber, and carbohydrate) and antioxidant activity of lotus receptacles (LRs) were studied. The LRs contained 88.25% carbohydrates, 22.90% crude fiber, 5.79% crude ash, 0.77% crude protein content, and 5.19% crude lipid on a dry weight basis. The ethanolic extract of lotus receptacle (ELR) exerted strong scavenging effects on 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical, hydroxyl radical (•OH), superoxide anion (O2-), and hydrogen peroxide (H2O2), as well as high reducing power. ELR pretreatment effectively elevated cell viability and decreased malondialdehyde (MDA) levels in H2O2-treated RAW 264.7 cells. Additionally, ELR had high phenolic and flavonoid contents with values of 318.15 mg gallic acid equivalent/g and 239.06 mg rutin equivalent/g, respectively. Moreover, the bioactivity-guided study of ELR resulted in the isolation and identification of quercetin-3-O-β-D-glucopyranoside (QOG), a dietary flavonoid. QOG exhibited strong DPPH free radical scavenging activity, with an IC50 value of 24.26 μM; docking studies revealed that QOC formed one hydrogen bond with LYS263 in the active site, suggesting it is, in part, responsible for the free activity of LR.

DNA Methylation in Peripheral Blood: A Potential Biomarker for Cancer Molecular Epidemiology

Li, Lian,Choi, Ji-Yeob,Lee, Kyoung-Mu,Sung, Hyuna,Park, Sue K.,Oze, Isao,Pan, Kai-Feng,You, Wei-Cheng,Chen, Ying-Xuan,Fang, Jing-Yuan,Matsuo, Keitaro,Kim, Woo Ho,Yuasa, Yasuhito,Kang, Daehee Japan Epidemiological Association 2012 Journal of epidemiology Vol.22 No.5

<P>Aberrant DNA methylation is associated with cancer development and progression. There are several types of specimens from which DNA methylation pattern can be measured and evaluated as an indicator of disease status (from normal biological process to pathologic condition) and even of pharmacologic response to therapy. Blood-based specimens such as cell-free circulating nucleic acid and DNA extracted from leukocytes in peripheral blood may be a potential source of noninvasive cancer biomarkers. In this article, we describe the characteristics of blood-based DNA methylation from different biological sources, detection methods, and the factors affecting DNA methylation. We provide a comprehensive literature review of blood-based DNA methylation as a cancer biomarker and focus on the study of DNA methylation using peripheral blood leukocytes. Although DNA methylation patterns measured in peripheral blood have great potential to be useful and informative biomarkers of cancer risk and prognosis, large systematic and unbiased prospective studies that consider biological plausibility and data analysis issues will be needed in order to develop a clinically feasible blood-based assay.</P>