Curdione Inhibits Proliferation of MCF-7 Cells by Inducing Apoptosis

Li, Juan,Bian, Wei-He,Wan, Juan,Zhou, Jing,Lin, Yan,Wang, Ji-Rong,Wang, Zhao-Xia,Shen, Qun,Wang, Ke-Ming Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.22

Background: Curdione, one of the major components of Curcuma zedoaria, has been reported to possess various biological activities. It thus might be a candidate anti-flammatory and cancer chemopreventive agent. However, the precise molecular mechanisms of action of curdione on cancer cells are still unclear. In this study, we investigated the effect of curdione on breast cancer. Materials and Methods: Xenograft nude mice were used to detect the effect of curdione on breast cancer in vivo; we also tested the effect of curdione on breast cancer in vitro by MTT, Flow cytometry, JC-I assay, and western blot. Results: Firstly, we found that curdione significantly suppressed tumor growth in a xenograft nude mouse breast tumor model in a dose-dependent manner. In addition, curdione treatment inhibited cell proliferation and induced cell apoptosis. Moreover, after curdione treatment, increase of impaired mitochondrial membrane potential occurred in a concentration dependent manner. Furthermore, the expression of apoptosis-related proteins including cleaved caspase-3, caspase-9 and Bax was increased in curdione treatment groups, while the expression of the anti-apoptotic Bcl-2 was decreased. Inhibitors of caspase-3 were used to confirm that curdione induced apoptosis. Conclusions: Overall, our observations first suggested that curdione inhibited the proliferation of breast cancer cells by inducing apoptosis. These results might provide some molecular basis for the anti-cancer activity of curdione.

Dammarane-type triterpene oligoglycosides from the leaves and stems of Panax notoginseng and their antiinflammatory activities

Li, Juan,Wang, Ru-Feng,Zhou, Yue,Hu, Hai-Jun,Yang, Ying-Bo,Yang, Li,Wang, Zheng-Tao The Korean Society of Ginseng 2019 Journal of Ginseng Research Vol.43 No.3

Background: Inflammation is widespread in the clinical pathology and closely associated to the progress of many diseases. Triterpenoid saponins as a key group of active ingredients in Panax notoginseng (Burk.) F.H. Chen were demonstrated to show antiinflammatory effects. However, the chemical structures of saponins in the leaves and stems of Panax notoginseng (PNLS) are still not fully clear. Herein, the isolation, purification and further evaluation of the antiinflammatory activity of dammarane-type triterpenoid saponins from PNLS were conducted. Methods: Silica gel and reversed-phase C8 column chromatography were used. Furthermore, preparative HPLC was used as a final purification technique to obtain minor saponins with high purities. MS, NMR experiments, and chemical methods were used in the structural identifications. The antiinflammatory activities of the isolated saponins were assessed by measuring the nitric oxide production in RAW 264.7 cells stimulated by lipopolysaccharides. Real-time reverse transcription polymerase chain reaction was used to measure the gene expressions of inflammation-related gene. Results: Eight new minor dammarane-type triterpene oligoglycosides, namely notoginsenosides LK1-LK8 (1-8) were obtained from PNLS, along with seven known ones. Among the isolated saponins, gypenoside IX significantly suppressed the nitric oxide production and inflammatory cytokines including tumor necrosis $factor-{\alpha}$, interleukin 10, interferon-inducible protein 10 and $interleukin-1{\beta}$. Conclusion: The eight saponins may enrich and expand the chemical library of saponins in Panax genus. Moreover, it is reported for the first time that gypenoside IX showed moderate antiinflammatory activity.

Comprehensive transcriptome and metabolome analysis reveals regulatory network for lignin biosynthesis in chicory sprouting

Li Juan,Guo Xiaozhu,Wang Gui,Zhou Zhenjie,Sun Xuemei 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.5

Chicory is consumed worldwide and is an important commercial crop. However, excess lignin deposition may reduce its quality. The comprehension of the molecular mechanism underlying the biosynthesis of lignin in chicory is currently inadequate. To address this, an integrative analysis of the metabolome and transcriptome profiles was performed in chicory sprout at three different stages. A total of 706 metabolites were identified, with cinnamic acid, ferulic acid, coniferaldehyde, and sinapaldehyde enriched during the growth of chicory sprouts. This suggested that these four metabolites may affect the growth of chicory sprouts. Transcriptome analysis demonstrated that the expression of most of the differentially expressed genes (DEGs) involved in lignin biosynthesis was up-regulated during chicory growth. Importantly, the metabolite and gene expression profiles were closely correlated during sprout development, especially in association with lignin biosynthesis. The results will serve as a reference for lignin biosynthesis in chicory and may also assist biologists in improving chicory quality.

大学生在线学习效果的多维度比较研究

Lijuan Huang,Xiaoyan Xu Institute of Management Research 2023 Journal of East Asia Management Vol.4 No.2

Online and offline mixed teaching mode has become an important way to promote the connotative development of higher education. Under the background that offline teaching has become mature, in order to further promote the development of online education, and promote the implementation of the mixed teaching mode, to mix and to provide basis for the construction of the mixed teaching mode, this study takes the online learning effect as the evaluation basis, adopts the online questionnaire survey to conduct statistical analysis of the online learning behavior of 2213 college students, and discusses the differentiation phenomenon of online learning groups from the micro, meso and macro perspectives. It is found that there are significant differences in the online learning effect of college students in terms of the type of learning platform, whether the school implements the online offline mixed teaching mode, education background, grade (bachelor's degree), and region. Colleges and universities should strengthen the promotion of online and offline mixed teaching mode; The online learning platform should improve the platform function and strengthen the functional differentiation design of learning resources for students. Education departments pay attention to the learning effect of online learners in different regions, and bridge the gap in regional education.

Characterization and evolutionary analysis of Brassica species-diverged sequences containing simple repeat units

Lijuan Wei,Meili Xiao,Annaliese S. Mason,Bi Ma,Kun Lu,Jia Na Li,Link Katrin,Donghui Fu 한국유전학회 2013 Genes & Genomics Vol.35 No.2

Brassica species, B. napus (canola), B. rapa and B. oleracea, are important sources of nutritionally valuable vegetable oil and protein-rich meal for animals and humans. Sequencing of the model plant Arabidopsis thaliana, has opened the way for investigations into the complex structure of the Brassica genomes, offering important insight into their evolution and composition. We use this sequence information for the characterization and functional analysis of SSR sequences that have diverged between the Brassica species. A total of 56 species-diverged sequences containing simple repeat units (SDS-SSR) of B. napus and its diploid progenitor species B. rapa and B. oleracea were isolated and characterized. Of these, 40 sequences showed homology with other Brassica sequences. Using the SSR Locator software, only 23 sequences were found to have SSRs,possibly due to the loss of SSR units in the process of species divergence. Sequence alignments with A. thaliana revealed that these species-diverged SSR sequences were responsible for Brassica divergence for differences between Brassica species in several genomic regions. Six active genes related to transferase, protein, transcription factor and retroelements were found in the SDS-SSRs. These results will further improve our understanding of the characteristics of species-diverged SSR fragments and their contribution to genome differentiation.

Selection of Salt-tolerant Strawberry Cultivars by Assessing Germination under Excess NaCl and Seawater Conditions

Lijuan Zhang,Sayaka Kitanishi,Yuichi Uno,Michio Kanechi,Noboru Inagaki 한국원예학회 2009 Horticulture, Environment, and Biotechnology Vol.50 No.5

STRONG CONVERGENCE THEOREM OF COMMON ELEMENTS FOR EQUILIBRIUM PROBLEMS AND FIXED POINT PROBLEMS

Lijuan Zhang,Zhibin Hou 영남수학회 2010 East Asian mathematical journal Vol.26 No.5

In this paper, we introduce an iterative method for finding a common element of the set of solutions of an equilibrium problem, the set of common fixed points of an asymptotically strictly pseudocontractive mapping in a Hilbert space. We show that the iterative sequence converges strongly to a common element of the two sets.

Polyaniline nanotubes doped with polymeric acids

Lijuan Zhang,Hui Peng,Jing Sui,Paul A. Kilmartin,Jadranka Travas-Sejdic 한국물리학회 2008 Current Applied Physics Vol.8 No.3,4

Self-assembled polyaniline (PANI) nanotubes were prepared in the presence of three dierent polymeric acids as dopants, namelypoly(4-styrenesulfonic acid) (PSSA), poly(acrylic acid) (PAA) and poly(methyl vinyl ether-alt-maleic acid) (PMVEA) by oxidative poly-merization using ammonium persulfate as the oxidant. The molecular structure of polymeric acids had a signicant eect on the mor-phology and size of the polyaniline nanotubes as determined by SEM. The PANI nanotubes were also characterized by FTIRspectroscopy and electron paramagnetic resonance spectroscopy.

A HYBRID ITERATIVE METHOD OF SOLUTION FOR MIXED EQUILIBRIUM AND OPTIMIZATION PROBLEMS

Lijuan Zhang,Junmin Chen 영남수학회 2010 East Asian mathematical journal Vol.26 No.1

In this paper, we introduce a hybrid iterative method for finding a common element of the set of solutions of a mixed equilibrium problem, the set of common fixed points of finitely many nonexpansive mappings and the set of solutions of the variational inequality for an inverse strongly monotone mapping in a Hilbert space. We show that the iterative sequences converge strongly to a common element of the three sets. The results extended and improved the corresponding results of L.-C.Ceng and J.-C.Yao.

STABILITY OF IMPULSIVE CELLULAR NEURAL NETWORKS WITH TIME-VARYING DELAYS

Lijuan Zhang,Lixin Yu 한국전산응용수학회 2011 Journal of applied mathematics & informatics Vol.29 No.5

This paper demonstrates that there is a unique exponentially stable equilibrium state of a class of impulsive cellular neural network with delays. The analysis exploits M-matrix theory and generalized comparison principle to derive some easily verifiable suffcient conditions for the global exponential stability of the equilibrium state. The results extend and improve earlier publications. An example with its simulation is given for illustration of theoretical results.