lncRNA Epigenetic Landscape Analysis Identifies <i>EPIC1</i> as an Oncogenic lncRNA that Interacts with MYC and Promotes Cell-Cycle Progression in Cancer

Wang, Zehua,Yang, Bo,Zhang, Min,Guo, Weiwei,Wu, Zhiyuan,Wang, Yue,Jia, Lin,Li, Song,Caesar-Johnson, Samantha J.,Demchok, John A.,Felau, Ina,Kasapi, Melpomeni,Ferguson, Martin L.,Hutter, Carolyn M.,Sof Cell Press 2018 Cancer Cell Vol. No.

<P><B>Summary</B></P> <P>We characterized the epigenetic landscape of genes encoding long noncoding RNAs (lncRNAs) across 6,475 tumors and 455 cancer cell lines. In stark contrast to the CpG island hypermethylation phenotype in cancer, we observed a recurrent hypomethylation of 1,006 lncRNA genes in cancer, including <I>EPIC1</I> (epigenetically-induced lncRNA1). Overexpression of <I>EPIC1</I> is associated with poor prognosis in luminal B breast cancer patients and enhances tumor growth <I>in vitro</I> and <I>in vivo.</I> Mechanistically, <I>EPIC1</I> promotes cell-cycle progression by interacting with MYC through <I>EPIC1</I>'s 129–283 nt region. <I>EPIC1</I> knockdown reduces the occupancy of MYC to its target genes (e.g., <I>CDKN1A</I>, <I>CCNA2</I>, <I>CDC20</I>, and <I>CDC45</I>). MYC depletion abolishes <I>EPIC1</I>'s regulation of MYC target and luminal breast cancer tumorigenesis <I>in vitro</I> and <I>in vivo</I>.</P> <P><B>Highlights</B></P> <P> <UL> <LI> LncRNAs show a hypomethylation phenotype, in contrast to a CIMP phenotype in cancer </LI> <LI> <I>EPIC1</I> promotes breast tumorigenesis through regulating cancer cell-cycle progression </LI> <LI> <I>EPIC1</I> directly interacts with MYC protein through <I>EPIC1</I>'s 129–283 nt region </LI> <LI> <I>EPIC1</I> regulates MYC targets by enhancing MYC occupancy on its target promoters </LI> </UL> </P> <P><B>Graphical Abstract</B></P> <P>[DISPLAY OMISSION]</P>

Vapour–liquid–solid growth of monolayer MoS₂ nanoribbons

Li, Shisheng,Lin, Yung-Chang,Zhao, Wen,Wu, Jing,Wang, Zhuo,Hu, Zehua,Shen, Youde,Tang, Dai-Ming,Wang, Junyong,Zhang, Qi,Zhu, Hai,Chu, Leiqiang,Zhao, Weijie,Liu, Chang,Sun, Zhipei,Taniguchi, Takaaki,Os Nature Publishing Group UK 2018 Nature Materials Vol.17 No.6

<P>Chemical vapour deposition of two-dimensional materials typically involves the conversion of vapour precursors to solid products in a vapour-solid-solid mode. Here, we report the vapour-liquid-solid growth of monolayer MoS2, yielding highly crystalline ribbons with a width of few tens to thousands of nanometres. This vapour-liquid-solid growth is triggered by the reaction between MoO3 and NaCl, which results in the formation of molten Na-Mo-O droplets. These droplets mediate the growth of MoS2 ribbons in the 'crawling mode' when saturated with sulfur. The locally well-defined orientations of the ribbons reveal the regular horizontal motion of the droplets during growth. Using atomic-resolution scanning transmission electron microscopy and second harmonic generation microscopy, we show that the ribbons are grown homoepitaxially on monolayer MoS2 with predominantly 2H-or 3R-type stacking. Our findings highlight the prospects for the controlled growth of atomically thin nano-structure arrays for nanoelectronic devices and the development of unique mixed-dimensional structures.</P>

Fabrication of polymer compound microlens by lens-on-lens microstructures

Zehua Xia,Yan Li,Xiaoya Su,Yanhua Han,Zhongyi Guo,Jian-Min Gao,Qiaoqun Sun,Shiliang Qu 한국물리학회 2017 Current Applied Physics Vol.17 No.1

We proposed a novel method for fabricating polymer compound microlenses (PCMLs) using micro-inkjet technique and subsequent curing process. Two different types of PCMLs with sandwich microstructure (PDMS-Glycerol-PDMS), concave and convex PCMLs, have been designed and fabricated in experiments. Convex PCML has two real images and two foci. The concave PCML has one real and one virtual focal planes, which can generate one real image and one virtual image respectively. Moreover, the diameter of concave PCML can be controlled by adjusting the curing time and temperature. The proposed method is simple, efficient and suitable for realizing large-scale high numerical aperture PCMLs array, which has potential applications in diverse optical systems such as optical storage and three-dimensional imaging.

A three-region movable-boundary helical coil once-through steam generator model for dynamic simulation and controller design

Wu Shifa,Li Zehua,Wang Pengfei,Su G.H.,Wan Jiashuang 한국원자력학회 2023 Nuclear Engineering and Technology Vol.55 No.2

A simple but accurate mathematical model is crucial for dynamic simulations and controller design of helical coil once-through steam generator (OTSG). This paper presents a three-region movable boundary dynamic model of the helical coil OTSG. Based on the secondary side fluid conditions, the OTSG is divided into subcooled region (two control volumes), two-phase region (two control volumes) and superheated region (three control volumes) with movable boiling boundaries between each region. The nonlinear dynamic model is derived based on mass, energy and momentum conservation equations. And the linear model is obtained by using the transfer function and state space transformation, which is a 37-order model of five input and three output. Validations are made under full-power steady-state condition and four transient conditions. Results show good agreements among the nonlinear model, linear model and the RELAP5 model, with acceptable errors. This model can be applied to dynamic simulations and controller design of helical coil OTSG with constant primary-side flow rate.

Experimental Investigation of Flow Control Technology for Grouting and Blocking of Flowing Water in Karst Conduits

Dongdong Pan,Zehua Bu,Haiyan Li,Zhenhao Xu,Jie Liu 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.8

Aiming at the grouting treatment in karst conduits with large flow and high velocity, we developed a simulation experiment system for conduit-type grouting blocking and investigated the blocking mechanism of flowing water grouting under the condition of flow control. Firstly, we studied the effect of flow control in conduit by controlling the opening of outlet valve and analyzed the variation law of velocity and pressure in conduits under different water heads, conduit diameters and valve opening quantitatively. Secondly, we conducted experiments of flowing water grouting under different flow control conditions, and revealed the influence law of flow control for flowing water grouting. The results show that: 1) with the increase of the intensity of flow control, the pressure in conduit increased gradually, and the velocity of flowing water decreased gradually. 2) When the volume ratio of water and cement was 1.2:1, the volume ratio of cement and sodium silicate was 2:1, the grouting volume was 540 L/h, and the flowing water velocity was 0.15 m/s, flowing water blocking under the condition of flow control achieved the optimal effect. 3) With flow control, the slurry deposition thickness increased more than 50% compared with no flow control; besides, the slurry retention rate increased, and the blocking effect significantly improved. The technology has been successfully applied in the grouting treatment project of China Resources Cement (Pingnan) Limestone Mine. And main water inflow points in the mine were blocked with an total amount of ~210,200 m3/d water reduction. This study provided some theoretical guidance and reference experience for treatment engineering of water inflow in karst area.

An Integrated Evaluation Method for the Grouting Effect in Karst Areas

Qing Jin,Zehua Bu,Dongdong Pan,Haiyan Li,Zhaofeng Li,Yichi Zhang 대한토목학회 2021 KSCE Journal of Civil Engineering Vol.25 No.8

Our proposed integrated evaluation method for the grouting effect in karst areas is based on the cloud model (CM), the analytic hierarchical process (AHP), and a fuzzy comprehensive evaluation system. Our method fully considers the fuzziness and randomness of the evaluation indices, and bridges the gap between qualitative information and a quantitative evaluation value. The evaluation index system for the grouting effect is established by integrating metrics related to the construction technology, apparent parameters, and observational data from inspection holes and geophysical exploration techniques. First, the weight of each index is calculated using the AHP, which has been modified by the CM. Then, we calculate the cloud model membership degree for each index. Finally, we determine the comprehensive evaluation level by examining the similarity measures between the various cloud models. In this case study, we use our integrated method to evaluate the initial results of the grouting treatment project in the China Resources Cement (Pingnan) Limestone Mine. Our results are validated by subsequent monitoring results for this project. This study provides valuable insight into the treatment of water inflow in karst areas.

Tidal Effect on Grouting in Karst Fracture with Flowing Water: Experimental Investigation and Its Application

Qing Jin,Zehua Bu,Dong-dong Pan,Xiaojie Gao,Pengshuai Yang,Haiyan Li,Zhaofeng Li,Zhenhao Xu 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.2

To address the problem on grouting control of water inflow disaster in coastal area, a caisson-type grouting experimental system of a single fracture in flowing water under tidal action was developed independently. Through the tide simulation system, the tidal action of different intensity and frequency was effectively described. Tide simulation system, fracture system, grouting system, water supply system and information monitoring system were combined to realize the visualization of the whole grouting process under tidal action. Furthermore, the grouting experiments of a plate fracture under different tidal action were developed and the blocking mechanism under tidal action was revealed. The conclusions were as follow: 1) Under constant water head, the slurry had three typical diffusion patterns, which were circular distribution, double U-shaped distribution and elliptical distribution respectively. 2) Under tidal action, the pressure showed a periodic fluctuation growth, and the growth rate slowed down with the increase of fracture aperture. 3) Strong tidal action would promote the diffusion of and increased the outflow amount of slurry, which was not conducive to grouting. Based on the water inflow treatment project of limestone mine in Guangxi, Through the field grouting tests, the 2# water inflow point was blocked. The research results have a certain reference value for the technology optimization of grouting engineering in coastal areas.

Influence of temperature on the development, reproduction, and life table of Calliptamus italicus (L.) (Orthoptera: Acridoidea)

Jin-Long Ren,Xiongbing Tu,Jing Ge,Li Zhao,Zehua Zhang 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.1

Calliptamus italicus is a migratory species of grasshopper that can cause serious damage to grasslands in Xinjiang, China. In this study,we examined the effects of different temperatures (23, 26, 29, 32, and 35 °C) on the lifecycle and life table parameters. The results showed that temperature strongly influences the growth and development of C. italicus. At temperatures ranging from 23 to 35 °C, the development time was negatively linearly related to temperature (P b 0.01). The time of development from an egg to an adult (pre-oviposition) ranged from86.8 d at 26 °C to 40.6 d at 35 °C, and no oviposition occurred at 23 °C. An average of 687.1 degree-days was required for complete development to occur above the lower threshold temperature (18.3 °C). Egg hatching rates and nymph and pre-adult survival rates were lowest at 23 °C (95.6%, 72.6%, and 0%, respectively) and highest at 32 °C (97.8%, 86.2%, and 88.7%, respectively). The mean total fecundity ranged from 41 eggs/female at 26 °C to 55 eggs/female at 32 °C. The sex ratio (% females) ranged from30 to 52%. The intrinsic rate of population increase (rm) at the different temperatures ranged from 0.0020 to 0.0447 d−1, with the highest value recorded at 32 °C. These results indicate that temperature significantly affects the biology of C. italicus and that the optimal temperature range for its development is 26–32 °C.

Low beta superconducting cavity system design for HIAF iLinac

Mengxin Xu,Yuan He,Shengxue Zhang,Lubei Liu,Tiancai Jiang,Zehua Liang,Tong Liu,Yue Tao,Chunlong Li,Qitong Huang,Fengfeng Wang,Hao Guo,Feng Bai,Xianbo Xu,Shichun Huang,Xiaoli Li,Zhijun Wang,Shenghu Zha Korean Nuclear Society 2023 Nuclear Engineering and Technology Vol.55 No.7

A superconducting ion-Linac (iLinac), which is supposed to work as the injector in the High Intensity heavy-ion Accelerator Facility project, is under development at the Institute of Modern Physics (IMP), Chinese Academy of Sciences. The iLinac is a superconducting heavy ion linear accelerator approximately 100 meters long and contains 96 superconducting cavities in two types of 17 cyromodules. Two types of superconducting resonators (quarter-wave resonators with a frequency of 81.25 MHz and an optimal beta β = v/c = 0.07 called QWR007 and half-wave resonators with a frequency of 162.5 MHz and an optimal beta β = 0.15 called HWR015) have been investigated. The cavity design included extensive multi-parameter electromagnetic simulations and mechanical analysis, and its results are described in details. The fundamental power coupler and cavity dynamic tuner designs are also presented in this article. The prototypes are under manufacturing and expected to be ready in 2023.