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Host Species as a Strong Determinant of the Intestinal Microbiota of Fish Larvae
Xuemei Li,Yuhe Yu,Weisong Feng,Qingyun Yan,Yingchun Gong 한국미생물학회 2012 The journal of microbiology Vol.50 No.1
We investigated the influence of host species on intestinal microbiota by comparing the gut bacterial community structure of four cohabitating freshwater fish larvae, silver carp, grass carp, bighead carp, and blunt snout bream, using denaturing gradient gel electrophoresis (DGGE) of the amplified 16S and 18S rRNA genes. Similarity clustering indicated that the intestinal microbiota derived from these four fish species could be divided into four groups based on 16S rRNA gene similarity, whereas the eukaryotic 18S rRNA genes showed no distinct groups. The water sample from the shared environment contained microbiota of an independent group as indicated by both 16S and 18S rRNA genes segments. The bacterial community structures were visualized using rank-abundance plots fitted with linear regression models. Results showed that the intestinal bacterial evenness was significantly different between species (P<0.05) and between species and the water sample (P<0.01). Thirty-five relatively dominant bands in DGGE patterns were sequenced and grouped into five major taxa: Proteobacteria (26), Actinobacteria (5), Bacteroidetes (1), Firmicutes (2), and Cyanobacterial (1). Six eukaryotes were detected by sequencing 18S rRNA genes segments. The present study suggests that the intestines of the four fish larvae, although reared in the same environment, contained distinct bacterial populations, while intestinal eukaryotic microorganisms were almost identical.
Xiao, Peng,Li, Qingyun,Joo, Yongjoon,Nam, Jutaek,Hwang, Sekyu,Song, Jaejung,Kim, Sungjee,Joo, Chulmin,Kim, Ki Hean Optical Society of America 2013 Optics letters Vol.38 No.21
<P>We report the feasibility of a novel contrast agent, namely 'smart' gold nanoparticles (AuNPs), in the detection of cancer cells with photothermal optical coherence tomography (PT-OCT). 'Smart' AuNPs form aggregation in low pH condition, which is typical for cancer cells, and this aggregation results in a shift of their absorption spectrum. A PT-OCT system was developed to detect this pH-induced aggregation by combining an OCT light source and a laser with 660 nm in wavelength for photothermal excitation. Optical detection of pH-induced aggregation was tested with solution samples at two different pH conditions. An increase in optical path length (OPL) variation was measured at mild acidic condition, while there was not much change at neutral condition. Detection of cancer cells was tested with cultured cell samples. HeLa and fibroblast cells, as cancer and normal cells respectively, were incubated with 'smart' gold nanoparticles and measured with PT-OCT. An elevated OPL variation signal was detected with the HeLa cells while not much of a signal was detected with the fibroblast cells. With the novel optical property of 'smart' AuNPs and high sensitivity of PT-OCT, this technique is promising for cancer cell detection.</P>
Providing survivability for virtual networks against substrate network failure
( Ying Wang ),( Qingyun Chen ),( Wenjing Li ),( Xuesong Qiu ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.9
Network virtualization has been regarded as a core attribute of the Future Internet. In a network virtualization environment (NVE), multiple heterogeneous virtual networks can coexist on a shared substrate network. Thus, a substrate network failure may affect multiple virtual networks. In this case, it is increasingly critical to provide survivability for the virtual networks against the substrate network failures. Previous research focused on mechanisms that ensure the resilience of the virtual network. However, the resource efficiency is still important to make the mapping scheme practical. In this paper, we study the survivable virtual network embedding mechanisms against substrate link and node failure from the perspective of improving the resource efficiency. For substrate link survivability, we propose a load-balancing and re-configuration strategy to improve the acceptance ratio and bandwidth utilization ratio. For substrate node survivability, we develop a minimum cost heuristic based on a divided network model and a backup resource cost model, which can both satisfy the location constraints of virtual node and increase the sharing degree of the backup resources. Simulations are conducted to evaluate the performance of the solutions. The proposed load balancing and re-configuration strategy for substrate link survivability outperforms other approaches in terms of acceptance ratio and bandwidth utilization ratio. And the proposed minimum cost heuristic for substrate node survivability gets a good performance in term of acceptance ratio.
Gradient index lens based combined two-photon microscopy and optical coherence tomography
Wang, Taejun,Li, Qingyun,Xiao, Peng,Ahn, Jinhyo,Kim, Young Eun,Park, Youngrong,Kim, Minjun,Song, Miyeoun,Chung, Euiheon,Chung, Wan Kyun,Ahn, G-One,Kim, Sungjee,Kim, Pilhan,Myung, Seung-Jae,Kim, Ki Hea The Optical Society 2014 Optics express Vol.22 No.11
Chaocheng Zhao,Shuaijun Wang,Qingyun Yan,Pei Dong,Yongqiang Wang,Fang Liu,Lin Li 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2018 NANO Vol.13 No.7
Rational design and construction of Z-scheme photocatalysts have received much attention in the fields of organic pollutants degradation and H2 evolution because of their potentials to solve the current environmental and energy crises. Herein, a novel nitrogen defects-rich 0D/2D α-Fe2O3/g-C3N4 Z-scheme photocatalyst was fabricated via a one-pot co-pyrolysis method. Quantum-sized α-Fe2O3 dots with a diameter of approximately 5 nm were uniformly distributed on the surface of 2D g-C3N4. Besides, the prepared 0D/2D α-Fe2O3/g-C3N4 composites exhibited excellent RhB degradation ability and simultaneous H2 evolution efficiency. In comparison with 2D g-C3N4, the RhB degradation rate and H2 evolution catalyzed by 0D/2D α-Fe2O3/g-C3N4 were enhanced 3.9 and 1.8 times, respectively. The improved photoredox activity was predominantly attributed to the formation of the nitrogen defects and a Z-scheme heterojunction. This study provides novel insights into the design and fabrication of nitrogen defects-rich g-C3N4-based Z-scheme photocatalysts for the simultaneous environmental purification and H2 evolution.
A Real-Time qPCR Assay to Quantify Ophiocordyceps sinensis Biomass in Thitarodes Larvae
Wei Lei,Shaosong Li,Qingyun Peng,Guren Zhang,Xin Liu 한국미생물학회 2013 The journal of microbiology Vol.51 No.2
Ophiocordyceps sinensis, an entomogenous fungus parasitic in the larvae of moths (Lepidoptera), is one of the most valuable medicinal fungi, and it only distributed naturally on the Tibetan Plateau. The parasitical amount of O. sinensis in various tissues of the host Thitarodes larvae has an important role in study the occurrence and developmental mechanisms of O. sinensis, but there no an effective method to detect the fungal anamorph. A real-time quantitative PCR (qPCR) system, including a pair of species-specific ITS primers and its related program, was developed for O. sinensis assay with high reliability and efficiency. A calibration curve was established and exhibited a very good linear correlation between the fungal biomass and the CT values (R2=0.999419)by the qPCR system. Based on this method, O. sinensis was detected rapidly in four tissues of its host caterpillars, and the results were shown as following: the maximum content of O. sinensis parasitized in the fat-body, and next came bodywall;both of them were much larger than that observed in the haemolymph and intestinal-wall. Taken together, these results show that qPCR assays may become useful tools for study on developmental mechanism of O. sinensis.
Qiu Mohan,Yu Chunlin,Zhu Shiliang,Liu Siyang,Peng Han,Xiong Xia,Chen Jialei,Jiang Xiaosong,Du Huarui,Li Qingyun,Zhang Zengrong,Yang Chaowu 한국유전학회 2022 Genes & Genomics Vol.44 No.11
Background: Long non-coding RNAs (lncRNAs) play an essential role in biological processes. However, the expression patterns of lncRNAs that regulate the non-Mendelian inheritance feather phenotypes remain unknown. Objective: This study aimed to compare the expression profiles of lncRNAs in the follicles of the late-feathering cocks (LC) and late-feathering hens (LH) that followed genetic rules and the early-feathering hen (EH) and early-feathering cock (EC) that did not conform to the genetic laws. Methods: We performed RNA sequencing and investigated the differentially expressed lncRNAs (DElncRNAs) between the early- and late-feathering chickens, which function by cis-acting or participate in the competing endogenous RNA (ceRNA) network. Results: A total of 53 upregulated and 43 downregulated lncRNAs were identified in EC vs. LC, and 58 upregulated and 109 downregulated lncRNAs were identified in EH vs. LH. The target mRNAs regulated by lncRNAs in cis were enriched in the pentose phosphate pathway, TGF-β signaling pathway and Jak-STAT signaling pathway in EC vs. LC and were associated with the TGF-β signaling pathway, Wnt signaling pathway, p53 signaling pathway and Jak-STAT signaling pathway in EH vs. LH. In addition, the lncRNA-mediated ceRNA regulatory pathways of hair follicle formation were mainly enriched in the TGF-β signaling pathway, Wnt signaling pathway, melanogenesis, and calcium signaling pathways. The levels of ENSGALG00000047626 were significantly higher in the late-feathering chickens than in the early-feathering chickens, which regulated the expression of SSTR2 by gga-miR-1649-5p. Conclusion: This study provides a novel molecular mechanism of lncRNA's response to the feather rate that does not conform to the genetic laws in chickens.