The Heterogeneity of Ovomucoid-Specific IgE Idiotype Is Associated With Egg Allergy Symptom Severity

Li Liuxu,Zhang Bei,Li Yifan,Huang Lunhui,Li Shaoshen,Liu Dandan,Yu Yang,Li Huiqiang 대한천식알레르기학회 2023 Allergy, Asthma & Immunology Research Vol.15 No.1

Immunoglobulin E (IgE)-mediated egg allergy presents as one of the most common food allergies. The level of specific IgE (sIgE) antibody is widely used as an important in vitro diagnostic indicator. However, sIgE antibody levels are often inconsistent with the clinical manifestations of patients. The heterogeneity of egg-specific IgE idiotypes (sIgE-IDs) may help reflect clinical egg allergy severity. Eight peptides were synthesized, corresponding to the linear epitopes of ovomucoid (OVM). The sIgE-IDs of egg-allergic patients were detected by enzyme-linked immunosorbent assay. Fresh peripheral blood was collected from patients with different heterogeneity strength of sIgE-ID, and egg extract was used as a stimulus to the basophil activation test (BAT). RBL-2H3 cells were sensitized with serum with different strength of sIgE-ID heterogeneity and the release rate of β-hexosaminidase was calculated. Among 75 patients with egg allergy, 24% had sIgE for all epitopes and 85% had sIgE for at least one epitope. Analysis of individual patients revealed differences in epitope recognition patterns among the patients, that is, heterogeneity in sIgE-ID. More importantly, the number of IgE-positive peptides had a strong correlation with allergic symptoms in egg-allergic patients (r = 0.706). BAT and RBL-2H3 cell degranulation confirmed that higher sIgE-ID heterogeneity strength was more effective in inducing effector cell responses. Our results suggest that the greater the heterogeneity strength of OVM-sIgE-ID, the more severe the allergic symptoms.

Efficient Synthesis of Long-wavelength Absorbing Cyanochlorophyll a Derivatives via Stereoselective Horner–Wadsworth–Emmons Reaction

Huiqiang Wu,Xiangmin Wang,Yang Liu,Yu Zhao,Xinyue Wang,Woo-Kyoung Lee,심영기,Il Yoon,Xinming Xu,Jiazhu Li 대한화학회 2020 Bulletin of the Korean Chemical Society Vol.41 No.5

In this article, we report the stereoselective Horner?Wadsworth?Emmons reaction of reactive carbonyl-substituted chlorophyll a derivatives with diethyl cyanomethylphosphonate. We found that the formyl or keto group at the chlorin periphery can react stereoselectively with phosphonate carbanions to produce a series of cyanomethylene-substituted chlorins with predominantly E geometry. Considering that no consistent records are available in the literature on the results of the allomerization of methyl pyropheophorbide a, we propose a reliable process for the transformation of this chlorophyll derivative. Our methodology represents a simple and efficient way for the preparation of novel, differently functionalized long-wavelength absorbing chlorins, those can be applied for photodynamic therapy, solar cells, and other relevant purposes.

한ㆍ중 FTA 실행가능한가?

곽근재(Kwak, Keun-Jae),이희강(Li, Huiqiang) 영남대학교 산경연구소 2010 영상저널 Vol.2 No.2

두 지역 간의 경제구조의 보완성 또는 두 지역의 서로 다른 산업에서의 특화패턴은 무역자유화의 가능한 충격을 분석하는데 있어서 매우 유용한 도구이다. 경제구조의 보완성 개념은 경제통합에 관한 초기 연구에 내포되어 있었지만 아마도 무역자유화에 관한 계량적 연구의 그늘에 가려 그 빛을 잃었다. 이 논문은 한국과 중국 간의 FTA의 근거를 위한 약간의 실마리를 제시한 후 몇 가지 지표--이 논문에서는 3가지 종류의 가법형 RCA, TCI, ESI--를 이용하여 두 나라간의 경제의 구조적 보완성을 분석한 후 한ㆍ중 FTA의 실행가능성을 평가하는 것을 연구의 목적으로 한다. 분석결과 한국과 중국은 대체로 보완적인 관계에 있으므로 한중 FTA의 실행가능성은 매우 높다고 평가할 수 있다. Economic structural complementarity between Region A and Region B, or the pattern the two regions specialize in different industries is a useful tool for the analysis of the likely impact of trade liberalization. Although implicit in earlier work on economic integration, this concept has been overshadowed subsequently, probably because of the econometric studies on trade liberalization. This paper aims to discuss first the rationale of FTA between Korea and China. Second, it aims to analyze economic structural complementarity between two countries using several indicators-additive RCA, TCI, and ESI. Analysis showed that the Korea and China are on the whole structurally complementary. Consequently, we can assess that the viability of the Korea-China FTA is very high.

Chitosan/hydroxyapatite composite coatings on porous Ti6Al4V titanium implants: in vitro and in vivo studies

Zhang, Ting,Zhang, Xinwei,Mao, Mengyun,Li, Jiayi,Wei, Ting,Sun, Huiqiang Korean Academy of Periodontology 2020 Journal of Periodontal & Implant Science Vol.50 No.6

Purpose: Titanium implants are widely used in the treatment of dentition defects; however, due to problems such as osseointegration failure, peri-implant bone resorption, and periimplant inflammation, their application is subject to certain restrictions. The surface modification of titanium implants can improve the implant success rate and meet the needs of clinical applications. The goal of this study was to evaluate the effect of the use of porous titanium with a chitosan/hydroxyapatite coating on osseointegration. Methods: Titanium implants with a dense core and a porous outer structure were prepared using a computer-aided design model and selective laser sintering technology, with a fabricated chitosan/hydroxyapatite composite coating on their surfaces. In vivo and in vitro experiments were used to assess osteogenesis. Results: The quasi-elastic gradient and compressive strength of porous titanium implants were observed to decrease as the porosity increased. The in vitro experiments demonstrated that, the porous titanium implants had no biological toxicity; additionally, the porous structure was shown to be superior to dense titanium with regard to facilitating the adhesion and proliferation of osteoblast-like MC3T3-E1 cells. The in vivo experimental results also showed that the porous structure was beneficial, as bone tissue could grow into the pores, thereby exhibiting good osseointegration. Conclusions: Porous titanium with a chitosan/hydroxyapatite coating promoted MC3T3-E1 cell proliferation and differentiation, and also improved osseointegration in vitro. This study has meaningful implications for research into ways of improving the surface structures of implants and promoting implant osseointegration.

Temperature characteristics of indentation rolling resistance of belt conveyor

Lidong Zhou,Zengfa Wu,Yongchao Li,Huiqiang Yao,Yuan Liu,Yuan Yuan,Wenjun Meng,Liangliang Han,Xueqin Cao 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.8

In order to study the influence of temperature on the indentation rolling resistance of belt conveyor, theoretical analysis, numerical simulation analysis and experimental study of conveyor belt indentation rolling resistance with temperature characteristics were carried out in this paper, and the influence rules of different factors on the indentation rolling resistance of belt conveyor were obtained. First, the three-component Maxwell model is chosen as the viscoelastic model for the conveyor belt rubber material, and the viscoelastic modulus function based on temperature effects is constructed from the DMA experimental data fitting. Second, we introduce the one-dimensional Winkler foundation model to derive a mathematical expression for the indentation rolling resistance based on temperature properties. Then, a mathematical model of the indentation rolling resistance of a conveyor belt with temperature characteristics is developed in MATLAB and numerical simulations are performed. Finally, using the existing experimental equipment to conduct experiments, the experimental results are compared with the numerical simulation result. The results show that the theoretical numerical simulation results of the indentation rolling resistance with temperature effect presented in this paper have a consistent change trend with the experimental results; at constant temperature and constant load, the indentation rolling resistance increases with increasing band velocity; at constant temperature and constant velocity, the indentation rolling resistance increases with increasing load; at constant load and speed, when the temperature is lower than 0 °C or higher than 25 °C, the rolling resistance increases with the increase of temperature, when the temperature is between 0 °C and 25 °C, the rolling resistance decreases gradually with the increase of temperature.