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      • KCI등재

        Phylogenetic and functional analysis of TGF-β/Smad2 pathway genes in cotton bollworm, Helicoverpa armigera

        Li Yan,Lu Qin,Luo Hailing,Xia Shunchao,Li Haiyin,Xu Weihua 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.4

        Transforming growth factor-beta (TGF-β) signaling pathway plays important roles in embryonic development, cell proliferation and tissue differentiation in vertebrates. Our previous studies demonstrated that TGF-β signal activates Smad1-POU-TFAM and PP2A-Akt pathways to regulate pupal diapause in Helicoverpa armigera. In this study, we investigated the function of TGF-β activates Smad2 pathway in H. armigera. Phylogenetic analysis of H. armigera TGF-β receptor I (TGFβRI), Smad2, Smad4 genes showed high conservation across species. In vitro experiments showed that TGFβRI was localized in the cell membrane where it binds Smad2 leading to the phosphorylation of Smad2. Smad4 was mainly localized in the cytoplasm, and bind to Smad2. Protein expression analysis showed that expression of TGFβRI, Smad4, Smad2, p-Smad2 were lower in diapause-destined pupae compared with nondiapause-destined pupae. Notably, treatment with 20-hydroxyecdysone (20E) increased expression of the above proteins. Inhibition of TGF-β/Smad2 signaling pathway delayed pupal development. These findings indicate that TGF-β/Smad2 pathway is involved in pupal development or diapause in H. armigera.

      • Generation Method of Autonomous Evasive Maneuver Strategy in Air Combat

        Lin Li,Zhen Yang,Zhixiao Sun,Guang Zhan,Haiyin Piao,Deyun Zhou 제어로봇시스템학회 2022 제어로봇시스템학회 국제학술대회 논문집 Vol.2022 No.11

        Air combat is usually a continuous process involving multiple rounds of missile attacks, UCAV(Unmanned Combat Aerial Vehicle) should consider the impact of maneuver on the entire air combat countermeasure mission rather than solely focusing on safety factors when evading incoming air to air missiles. In this paper, a method of generating autonomous evasive maneuver strategy in UCAV air combat is proposed under the condition of miss distance and energy dissipation. Firstly, the UCAV-missile three-dimensional space pursuit model and the UCAV autonomous evading state space, action space and reward function model are established. Dueling DDQN (Double Deep Q Network) algorithm is used to solve the model, which integrates Double DQN and Dueling DQN Network model. Simulation experiments and analysis show that the method proposed in this paper has significant effectiveness in solving the problem of autonomous evasive maneuvers in air combat under the conditions of miss distance and energy dissipation tactical requirements. The evasive maneuvers strategy obtained can reflect the two tactical requirements of air combat while ensuring the safety of UCAV itself.

      • KCI등재

        Biochemical Properties of Skin Collagens Isolated from Black Carp (Mylopharyngodon piceus)

        Yuanjun Jia,Haibo Wang,Haiyin Wang,Yunyan Li,Min Wang,Jian Zhou 한국식품과학회 2012 Food Science and Biotechnology Vol.21 No.6

        Acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) were extracted with the yield of 15.5 and 26.5% on the basis of dry weight from the skin of black carp (Mylopharyngodon piceus). ASC and PSC were similar in amino compositions and subunit compositions,but they were slightly different in the secondary structure. Both of ASC and PSC, either rehydrated in distilled water or in acetic acid, had a lower denaturation temperature than pig skin collagen (PPSC), but their denaturation temperature were higher than that of collagens from other freshwater fish. Intrinsic viscosity of different collagens decreased in the order of PPSC>PSC>ASC. Both of ASC and PSC showed only partial degradation in collagenase solution,but they revealed more sensitive to collagenase compared with PPSC. Black carp skin collagens did not induce a significant cytotoxic effect according to the results of in vitro cytotoxicity test.

      • KCI등재

        Influence of Hybrid Ratios on Shape Memory Properties of Basalt/Carbon Fiber Hybrid Composites with Graphene Oxide Prepared by VIHPS

        Yi Xu,Yuqin Ma,Yu Yang,Gangfeng Wang,Wei Xu,Fei Li,Haiyin Guo,Yatao Li 한국섬유공학회 2023 Fibers and polymers Vol.24 No.8

        A study on the shape memory properties of 6 layers bi-directional basalt fiber/carbon fiber hybrid composites with graphene oxide (GO) was presented in this paper. The hybrid composites were prepared by the vacuum infiltration hot pressing system (VIHPS). The macroscopic appearance of these composites was desirable, and their microstructure was satisfactory. According to the test results, with the content of basalt fiber decreased, the shape fixation ratio decreased, while the shape recovery ratio and shape recovery force all increased. The basalt fiber composite with 6 layers basalt fiber (B6) had a maximum shape fixation ratio of 94.94%, a minimum shape recovery ratio of 85.52%, and a minimum recovery force of 1.36 N. Compared with B6, the shape fixation ratio of the carbon fiber composite with 6 layers carbon fiber (C6) decreased by 10.43%, which was 84.51%. The shape recovery ratio and shape recovery force were increased by 9.60% and 3.31 times, and their values were 95.12% and 5.86 N, respectively. In addition, according to the temperature-recovery force curves, it was found that the seven groups of composites all reached their maximum shape recovery force when the temperature was about 110 °C. When the temperature was between 50 °C and 80 °C, the recovery force of the composite increased greatly, and then, the recovery force increased slightly. However, when the temperature was above the glass transition temperature (Tg), the recovery force gradually decreased.

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