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Comparison of Spermatozoa Recovery Methods on Cauda Epididymal Sperm of Hanwoo Bulls
Sung-Sik Kang,Ui-Hyung Kim,Min-Hyeong Jeon,Myung-Suk Lee,Sang-Rae Cho 한국수정란이식학회 2018 한국동물생명공학회지 Vol.33 No.4
In this study, two epididymal spermatozoa recovery methods in relation to sperm number, motility, viability and acrosome reaction were examined. Seven bulls were castrated and 7 testicles with epididymides were transferred to the laboratoy. Epididymis in each bull was randomly used for flushing and mincing methods with semen extender (Optixcell, IMV, France). The recovered spermatozoa with adjusted sperm concentration to 40 × 106 cells/mL was diluted with optixcell and cryopreserved. In experiment 1, the difference in the total number of spermatozoa using flushing and mincing methods was insignificant (2570.0 and 2505.2 × 106 cells/mL, respectively). For experiment 2, the percentage of motile spermatozoa and motility parameters between flushing and mincing methods were studied through the use of sperm class analyzer after frozen-thawing. The percentage of total motile sperm between flushing and mincing methods was almost the same with 89.5±12.8 and 91.4±7.9%, respectively. The same is the case with experiment 3 wherein the viability and acrosomal integrity of frozen-thawed epididymal spermatozoa by flushing and mincing was insignificantly different. The results from the study showed that both flushing and mincing methods can be used for epididymal spermatozoa recovery in bull.
Bovine leukosis in a slaughtered cow
Lee, Sang-Myung,Choi, Hae-Yeun,Kwak, Hak-Koo,Jung, Chan-Woo,Han, Seong-Tae,Park, Sun-Jun,Yoon, Ho-Gyu,Jang, Rae-Hun The Korean Society of Veterinary Service 2007 韓國家畜衛生學會誌 Vol.30 No.4
A slaughtered 3 years old cow was diagnosed as bovine leukosis by postmortem inspection, agar gel immunodifusion (AGID) test, and polymerase chain reaction (PCR). It showed no special clinical signs except emaciation. Grossly, we observed enlargement of mediastinal and pelvic lymph nodes, and tubercle -like mass on pleura and lung surface, and masses on right atrium and ventricle.
Sung-Sik Kang,Sang-Rae Cho,Ui-Hyung Kim,Chang-Seok Park,Hyeong-Cheol Kim,Ki-Yong Chung,Seok-Dong Lee,Sun-Sik Jang,Gi-jun Jeon,Sidong Kim,Myeong-Suk Lee,Byoung-Chul Yang 한국수정란이식학회 2016 한국동물생명공학회지 Vol.31 No.2
The recovery of epididymal sperm in animals is considered as one of the important tools to preserve high value or endangered species. However, there are no appropriate castrating indicators such as months of age in bull, sperm morphology, and motility, particularly in young Korean native bull (Hanwoo). Therefore, this study aimed to investigate sperm number, morphology, and motility of sperm in the epididymis tail of young Hanwoo bulls at 8 and 15 months of age. After castration, epididymal tails were collected and minced with blades to recover sperm. In experiments 1 and 2, sperm number, morphology, and motility were examined. Total number of sperm and percentage of normal sperm from bulls at 8 months of age was lower than that of bulls at 15 months of age after collection (P<0.05). Percentage of abnormal head, tail, proximal cytoplasmic droplet, dead and damaged acrosome of sperm from bulls at 8 months of age were higher than those of bulls at 15 months of age (P<0.05). In experiment 3, sperm motility from bulls at 8 and 15 months of age were examined before freezing and after thawing. Frozen-thawed sperm at 8 months of age showed low total motility and motile sperm with ≥ 25 μm/sec compared to those at 15 months of age and commercially-used sperm (P<0.05). In conclusion, sperm derived from the epididymal tail of bulls at 8 months of age showed high abnormal morphology and poor motility, which are not adequate for AI and IVF. On the other hand, sperm derived from the epididymal tail of bulls at 15 months of age showed high normal morphology and motility.
Silkworm Thioredoxin Peroxidase Is Induced by External Temperature Stimulus and Viral Infection
Lee Kwang-Sik,Kim Seong-Ryul,Park Nam-Sook,Kim Ik-Soo,Kang Pil-Dong,Sohn Bong-Hee,Choi Kwang-Ho,Kang Seok-Woo,Je Yeon-Ho,Lee Sang-Mong,Sohn Hung-Dae,Jin Byung-Rae 한국응용곤충학회 2004 한국응용곤충학회 학술대회논문집 Vol.2004 No.1
Lee,Sang-Rae 大韓口腔顎顔面 放射線學會 1996 Imaging Science in Dentistry Vol.26 No.1
The system of Oral Maxillofacial Radiology education, radiographic equipments and oral health care were evaluated in Korea. The objectives, the length, curriculum guidelines, instructional method and teaching means for undergraduate, and postgraduate education in 9 dental colleges were described. To investigate the radiographic equipments, surveys were received from 6 Dental College Hospitals(DCH), 23 Dental Hospitals(DH) and 373 Dental Clinics(DC). And the results of oral health care were obtained from the surveys of Kyunghee Dental College Hospital(KDCH), 14 Dental Hospitals and 52 Dental Clinics.
PRIMARY MALIGNANT MELANOMA OF THE NOSE AND PARANASAL SINUSES ; REPORT OF A CASE
LEE, Joo Hyun,HWANG, Eui Hwan,LEE, Sang Rae,KWON, Ki Jeong 大韓口腔顎顔面 放射線學會 1993 Imaging Science in Dentistry Vol.23 No.2
악성 흑색종은 멜라닌모세포로부터 유래되는 종양으로서, 이의 병인에 대하여서는 현재까지 확실히 구명된 바 없으나, 외상이나 만성 자극 등에 의하여 발생되는 것으로 알려져 있다. 이는 전체 악성 종양의 1.2%를 차지하는 발생빈도가 매우 낮은 질환으로서, 대부분 피부에서 발생되고, 점막에서의 발생은 매우 드물다. 특히 비강과 부비동에서 원발되는 경우는 전체 악성 흑색종의 0.6% - 0.7%에 불과하며, 예후가 극히 불량하다. 비강과 부비동의 원발성 악성 흑색종은 50세 - 70세 사이에서 호발되고, 사춘기이전에는 거의 발생되지 않으며, 남녀 성차는 없는 것으로 알려져 있다. 또한 비폐색과 비출혈이 가장 흔한 증상이며, 간혹 비루, 안모종창, 두통등을 호소하기도 하나, 종물이 상당한 크기로 성장되기 전에는 특이한 임상증상을 보이지 않으므로 이의 조기발견 및 적절한 치료가 요구된다. 저자들은 최근 안모종창과 동통을 주소로 내원한 36세 여자 환자의 비강과 부비동에 원발된 악성 흑색종 1예를 경험하였기에 문헌고찰과 함께 이를 보고하는 바이다.