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Characterization of the N - ras proto - oncogene by PCR - cDNA amplification
Kim, Young Soo,Min, Kyung Rak,Gerald N Wogan 한국유전학회 1989 Genes & Genomics Vol.11 No.4
A 600-base pair cDNA containing the entire rat N-ras coding exonic sequence was amplified by a transcript-PCR method. Sequence comparison of mammalian N-ras genes revealed that the rat N-ras proto-oncogene shared extensive sequence homology with human and mouse N-ras proto-oncogenes. The homology of N-ras in human, mouse, and rat was 90 to 95% in nucleotide sequence. Rat N-ras exhibited 4 amino acid differences when compared to mouse N-ras, 3 of which were in the C-terminal region encoded by exon IV. An evolutionary divergence at position 69 was also found, in which rat N-ras contained glycine but other known mammalian ras proteins including H-, K-, and N-ras have aspartate at position 69 in this highly conserved region. Starting from total RNA derived from the liver of Fischer rat, a double strand cDNA containing two unique endonuclease sites in its termini was obtained within 5 hrs. The terminal endonuclease sites were used for direct cloning into M13 to obtain a library enriched for N-ras proto-oncogene. Screening assay of plaques and sequence analysis of individual cloned cDNA product were used to evaluate the efficency and fidelity of this cDNA amplification-direct M13 cloning procedure. The target N-ras cDNA clones were detected in 5% of the total screened plaques and no sequence errors introduced by the in vitro amplification procedure were observed. This method proved satisfactory for obtaining a complete cDNA sequence of the N-ras proto-oncogene from nanogram quantities of the total liver RNA.
Kim, Tae-Sung,Park, Jung-Eun,Shukla, Anil,Choi, Sunho,Murugan, Ravichandran N.,Lee, Jin H.,Ahn, Mija,Rhee, Kunsoo,Bang, Jeong K.,Kim, Bo Y.,Loncarek, Jadranka,Erikson, Raymond L.,Lee, Kyung S. National Academy of Sciences 2013 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.110 No.50
<P>Centrosomes play an important role in various cellular processes, including spindle formation and chromosome segregation. They are composed of two orthogonally arranged centrioles, whose duplication occurs only once per cell cycle. Accurate control of centriole numbers is essential for the maintenance of genomic integrity. Although it is well appreciated that polo-like kinase 4 (Plk4) plays a central role in centriole biogenesis, how it is recruited to centrosomes and whether this step is necessary for centriole biogenesis remain largely elusive. Here we showed that Plk4 localizes to distinct subcentrosomal regions in a temporally and spatially regulated manner, and that Cep192 and Cep152 serve as two distinct scaffolds that recruit Plk4 to centrosomes in a hierarchical order. Interestingly, Cep192 and Cep152 competitively interacted with the cryptic polo box of Plk4 through their homologous N-terminal sequences containing acidic-alpha-helix and N/Q-rich motifs. Consistent with these observations, the expression of either one of these N-terminal fragments was sufficient to delocalize Plk4 from centrosomes. Furthermore, loss of the Cep192- or Cep152-dependent interaction with Plk4 resulted in impaired centriole duplication that led to delayed cell proliferation. Thus, the spatiotemporal regulation of Plk4 localization by two hierarchical scaffolds, Cep192 and Cep152, is critical for centriole biogenesis.</P>
Kyung N. Kim,Ki M. Chung KOREAN ACADAMY OF ORAL BIOLOGY 2009 International Journal of Oral Biology Vol.34 No.3
Whole-body γ-irradiation(WBI), which produces an oxidative stress, is reported to attenuate the acute antinociceptive action of morphine (a μ-opioid receptor agonist), but not DPLPE (a δ-opioid receptor agonist), in mice. Recently, we also reported that antinociceptive effect of morphine, but not β-endorphin (a novel ε-opioid receptor agonist), was attenuated by oxidative stress. These findings prompted us to investigate the effect of WBI on the antinociception of morphine and β-endorphin in mice. Mice were exposed to WBI (5 Gy) from a 60 Co gamma-source and tested 2 hours later for antinociception produced by intracerebroventricular administration of morphine or β-endorphin using the hot water tail-immersion and the writhing tests. WBI significantly attenuated the antinociception produced by morphine only in the hot water tail-immersion test, whereas the antinociception of β-endorphin was significantly potentiated by WBI in both tests. These results demonstrate a differential sensitivity of μ- and ε-opioid receptors to WBI, and support the hypothesis that morphine and β-endorphin administered supraspinally produce antinociception by different neuronal mechanisms.
( Jae Hwi Han ),( Hyun Jung Kim ),( Jae Gwang Song ),( Jae Hyuk Yang ),( Nikhl N Bhandare ),( Aldrich Raymund Fernandez ),( Hyung Jun Park ),( Kyung Wook Nha ) 대한슬관절학회 2015 대한슬관절학회지 Vol.27 No.4
Purpose: Bone grafting in opening wedge high tibial osteotomy (OWHTO) is still controversial. The purpose of this study is to compare the radiological outcomes of OWHTO with bone graft (autogenous, allogenous, and synthetic bone graft) and those without bone graft. Materials and Methods: PubMed, MEDLINE, EMBASE and Cochrane Register of Studies databases were searched using specific inclusion and exclusion criteria for radiological studies involving OWHTO with bone graft and without bone graft groups. All reported delayed union, nonunion and correction loss were analyzed. Data were searched from the time period of January 2000 through July 2014. In addition, a modified Coleman methodology score (CMS) system was used to assess the methodological quality of the included studies. Results: Twenty-five studies with a mean CMS value of 77 (range, 61 to 85 score) were included. In total, 1,841 patients underwent OWHTO using 4 different procedures for bone graft: autobone graft (n=352), allobone graft (n=547), synthetic bone graft (n=541) and no bone graft (n=401). There was a similar tendency for delayed union, nonunion and correction loss rate among the osteotomy space filling methods. Conclusions: The meta-analysis showed there was a similar tendency for radiological union and correction maintenance among patients undergoing OWHTO regardless of the type of bone in all of the studies. However, the currently available evidence is not sufficient to strongly support the superiority of OWHTO with bone graft to OWHTO without bone graft.
In Eun Jin,Min Kyung Joo,Bak Sang-In,Kim Do Yoon,함철민,Shim Chungbo,Zhou Yujie,Hong Seung-Woo,Park Tae-Sun,Shin Jae Won,Bhoraskar V. N. 한국물리학회 2020 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.77 No.9
The cross sections of 89Y(n,3n)87m,87gY and 209Bi(n,4n)206Bi reactions at a neutron energy of 30 MeV are measured by making use of neutron beams of continuous energy spectra and a subtraction method. By impinging proton beams of 30 and 35 MeV to a thick beryllium target, neutron beams of continuous and broad energy spectra are produced and are guided to Y and Bi sample targets. The difference between the two neutron spectra generated by two neighboring proton energies is found to be peaked in a narrow energy range and thus can be regarded as quasi-monoenergetic, which can be used to extract (n,xn) cross sections. The uncertainty in the neutron fluence is reduced by analyzing the activities of aluminum and niobium reference samples placed on top of the Y and Bi samples. The use of a subtraction method by employing neutron beams of continuous energy spectra gives us the 89Y(n,3n)87m,87gY and 209Bi(n,4n)206Bi cross sections in fair agreement with the existing experimental data and nuclear data libraries.
Kim, Chongtae,Jeong, Da Eun,Heo, Sungeun,Ji, Eunbyul,Rho, Jun Gi,Jung, Myeongwoo,Ahn, Sojin,Kim, Ye‐,Jin,Kim, Yong‐,Sung,Nam, Suk Woo,Kulkarni, Rohit N,Lee, Kyoung Bun,Lee, Eun Kyung,Kim, Longman 2018 The Journal of pathology Vol.246 No.2
<P><B>Abstract</B></P><P>For the majority of patients diagnosed with pancreatic neuroendocrine tumors (NETs), there is significant malignant potential with a poor prognosis; however, the molecular abnormalities and pathogenesis of pancreatic NETs have not been firmly established. Here, we report that loss of expression of the RNA‐binding protein HuD correlates with low p27<SUP>Kip1</SUP> (p27) levels and poor prognosis in pancreatic NETs. HuD expression was frequently lost in many human pancreatic NETs, and these pancreatic NETs showed aggressive clinicopathological phenotypes with low p27 levels, increased tumor size, higher World Health Organization grade and pT stage of the tumor, and the presence of angioinvasion. Furthermore, loss of HuD was an independent, progression‐free prognostic factor in multivariate survival analysis. However, the level of HuR, a member of the same Hu protein family as HuD, was not significantly correlated with pancreatic NET size and progression. Mechanistically, HuD enhanced <I>p27</I> mRNA translation by interacting with both the 5′‐untranslated region (UTR) and the 3′‐UTR of <I>p27</I> mRNA, and consequently suppressed cell cycle progression and tumor growth. In addition, HuD competed with miR‐30a‐3p for binding to the 3′‐UTR of <I>p27</I> mRNA, suggesting an interplay between HuD and miR‐30a‐3p in controlling <I>p27</I> translation. Our results identify HuD as a pivotal suppressor of pancreatic NET growth, and suggest that HuD has potential value as a prognostic factor of pancreatic NETs. Copyright © 2018 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.</P>
( Sue N. Park ),( Yhun Y. Sheen ),( Ki Y. Kim ),( Ji H. Kim ),( Kyoung J. Kwon ),( Seo Y. Go ),( Kyung N. Min ),( Woo S. Lee ) 한국응용약물학회 2006 Biomolecules & Therapeutics(구 응용약물학회지) Vol.14 No.4
1,2-Dibromoethane (DBE) has been widely used as a soil fumigant, an additive to leaded gasoline and an industrial solvent. In this study, we have carried out in vitro genetic toxicity test of 1,2-dibromoethane and microarray analysis of differentially expressed genes in response to 1,2-dibromoethane. 1,2-Dibromoethane showed mutations in base substitution strain TA1535 both with and without exogenous metabolic activation. 1,2-Dibromoethane showed mutations in frame shift TA98 both with and without exogenous metabolic activation. 1,2-Dibromoethane showed DNA damage based on single cell gel/comet assay in L5178Y cells both with and without exogenous metabolic activation. 1,2-Dibromoethane increased micronuclei in CHO cells both with and without exogenous metabolic activation. Microarray analysis of gene expression profiles in L5l78Y cells in response to 1,2-dibromoethane selected differentially expressed 241 genes that would be candidate biomarkers of genetic toxic action of 1,2-dibromoethane.
가와사키병 환아에서 아스피린 치료 후 나타난 호산구증가증 1예
설인숙 ( N Suk Sol ),최명현 ( Myung Hyun Choi ),김민정 ( Min Jung Kim ),김윤희 ( Yoon Hee Kim ),이희선 ( Hee Seon Lee ),한윤기 ( Yoon Ki Han ),김기환 ( Ki Hwan Kim ),김경원 ( Kyung Won Kim ),손명현 ( Myung Hyun Sohn ),김규언 ( Kyu 대한천식알레르기학회(구 대한알레르기학회) 2014 Allergy Asthma & Respiratory Disease Vol.2 No.2
Drug hypersensitivity is one of drug adverse reactions that develop in susceptible patients following exposure to certain drugs and cannot be predicted from the known pharmacology of a drug. Severe hypersensitivity is associated with high morbidity and mortality. Although the issue of hypersensitivity to nonsteroidal anti-inflammatory drugs (NSAIDs) has been largely investigated in adults, data related to NSAIDs hypersensitivity is insufficient in childhood. And in spite of the recommendation to avoid use of aspirin due to Reye syndrome in children, aspirin is one of major treatment along with intravenous immunoglobulin in Kawasaki disease. We report a case of a 10-month-old boy who underwent intravenous immunoglobulin and aspirin treatment for Kawasaki disease, and subsequently revealed severe leukocytosis and eosinophilia. To our knowledge, there have been no previous reportsof aspirin-induced eosinophilia in Korea. (Allergy Asthma Respir Dis 2014;2:142-145)