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칼슘제 수관살포가 참다래의 과실 품질과 저장에 미치는 영향
임경호,나양기,임동근,마경철,조윤섭,김월수,이상현,박용서 全南大學校 農業科學技術硏究所 2001 農業科學技術硏究 Vol.36 No.-
This study were carried out to improve Kiwifruit quality and storage life. Three kinds of calcium compound were sprayed and calcium content of fruits, weight loss during fruit storage and fruit quality were investigated. Calcium contents within leaves and fruit were lower in Clef-non treatment than that of control. The calcium content in fruit pericarp of Kalk-H and CaCl2 was 0.04 to 0.05% higher than that of control. Fruit weight and soluble solids content at harvest was a little higher but acidity and fruit hardness was lowered. Fruit weight loss of Kalk-H and CaCl2 treatment was 1.39 to 1.53% lower than that of control during storage. The soluble solids of ripen fruit was 1.0 to 1.3% higher in all treatment and 0.8% higher in Kalk-H treatment in 120 of after storage. Fruit hardness of control fruit was higher at harvest but that of CaCl2 treatmented fruit was 0.39㎏/φ 5㎜ higher in 120 days of storage.
Kim, Min-Kyeong,Song, Ji-Yang,Koh, Dong-In,Kim, Jin Young,Hatano, Masahiko,Jeon, Bu-Nam,Kim, Min-Young,Cho, Su-Yeon,Kim, Kyung-Sup,Hur, Man-Wook American Society for Biochemistry and Molecular Bi 2019 The Journal of biological chemistry Vol.294 No.1
<P>Even in the face of physiological DNA damage or expression of the tumor suppressor protein p53, B cell CLL/lymphoma 6 (BCL6) increases proliferation and antagonizes apoptotic responses in B cells. BCL6 represses <I>TP53</I> transcription and also appears to inactivate p53 at the protein level, and additional findings have suggested negative mutual regulation between BCL6 and p53. Here, using <I>Bcl6</I><SUP>−/−</SUP> knockout mice, HEK293A and HCT116 <I>p53</I><SUP>−/−</SUP> cells, and site-directed mutagenesis, we found that BCL6 interacts with p53 and thereby inhibits acetylation of Lys-132 in p53 by E1A-binding protein p300 (p300), a modification that normally occurs upon DNA damage–induced cellular stress and whose abrogation by BCL6 diminished transcriptional activation of p53 target genes, including that encoding caspase-1. Conversely, we also found that BCL6 protein is degraded via p53-induced, caspase-mediated proteolytic cleavage, and the formation of a BCL6–p53–caspase-1 complex. Our results suggest that p53 may block oncogenic transformation by decreasing BCL6 stability via caspase-1 up-regulation, whereas aberrant BCL6 expression inactivates transactivation of p53 target genes, either by inhibiting p53 acetylation by p300 or repressing <I>TP53</I> gene transcription. These findings have implications for B cell development and lymphomagenesis.</P>
Kim, Kwang Il,Park, Jae Jun,Lee, Yong Jin,Lee, Tae Sup,Woo, Kwang Sun,Song, Inho,Kim, Kyeong Min,Choi, Chang Woon,Lim, Sang Moo,Kang, Joo Hyun Informa Healthcare 2011 International journal of radiation biology Vol.87 No.12
<P><I>Purpose</I>: Multimodality imaging contributes to the activation of translational research by compensating for its weak points. Herein, we developed a noninvasive dual-reporter gene system for nuclear and optical imaging.</P><P><I>Materials and methods</I>: We constructed a fusion reporter vector concurrently expressing the human sodium/iodide symporter (hNIS) and monomeric red fluorescent protein (mCherry), and evaluated the function of this fusion reporter system under in vitro and in vivo conditions.</P><P><I>Results</I>: The expression of hNIS/mCherry fusion gene was confirmed in transfected cells using reverse transcription polymerase chain reaction (RT-PCR) and Western blotting. As the numbers of cells increased, the fluorescence and <SUP>125</SUP>I uptake increased in the hNIS/mCherry-transfected cells, and a high correlation between fluorescence intensity and radioactivity was noted. The fluorescence intensities and radioactivity signals were also well-correlated in HT-29-hNIS/mCherry tumors (R<SUP>2</SUP> == 0.9304) in in vivo fluorescence and gamma camera imaging.</P><P><I>Conclusions</I>: The dual-reporter imaging method using hNIS and mCherry genes reflected tumor extent as well as viable cell numbers, and correlated well with one another. This suggests that the hNIS/mCherry dual-reporter system can be a useful tool for multi-modal imaging.</P>
Kim, Min-Kyeong,Jeon, Bu-Nam,Koh, Dong-In,Kim, Kyung-Sup,Park, So-Yoon,Yun, Chae-Ok,Hur, Man-Wook American Society for Biochemistry and Molecular Bi 2013 The Journal of biological chemistry Vol.288 No.10
<P>The human POZ domain and Krüppel-like zinc finger (POK) family proteins play important roles in the regulation of apoptosis, cell proliferation, differentiation, development, oncogenesis, and tumor suppression. A novel POK family transcription factor, BTB/POZ and zinc finger domains factor on chromosome 1 (BOZF-1; also called ZBTB8A), contains a POZ domain and two C<SUB>2</SUB>H<SUB>2</SUB>-type Krüppel-like zinc fingers and is localized at nuclear speckles. Compared with paired normal tissues, BOZF1 expression is increased in cancer tissues of the prostate, breast, and cervix. BOZF1 repressed the transcription of <I>p21WAF</I>/<I>CDKN1A</I> by acting on the proximal promoter concentrated with Sp1-binding GC boxes. BOZF1 competed with Sp1 in binding to GC boxes 1–5/6 of the <I>CDKN1A</I> proximal promoter. In addition, BOZF1 interacted with p53 and decreased the acetylation of p53 by p300, which reduced the DNA binding activity of p53 at the far distal p53-binding element. BOZF1 blocked the two major molecular events that are important in both constitutive and inducible transcription activation of <I>CDKN1A</I>. BOZF1 is unique in that it bound to all the proximal GC boxes to repress transcription, and it inhibited p53 acetylation without affecting p53 stability. BOZF1 might be a novel proto-oncoprotein that stimulates cell proliferation.</P>
Kim, Byong-Kak,Choi, Eung-Chil,Chung, Kyeong-Soo,Park, Hee-Ju,Kim, Hye-Ryoung,Kim, Yang-Sup,Park, Yong-Hwan,Shim, Mi-Ja The Pharmaceutical Society of Korea 1983 Archives of Pharmacal Research Vol.6 No.2
To find anititumor metabolites in Korean basidiomycetes, the shake-cultured mycelia of eight of the higher fungi were extracted with hot water and the extracts, after being partially purified, were subjected to in vivo antitumor test. When administered i. p. at the dose of 30mg/kg/day for ten consecutive days into the female ICR mice, which had been implanted with $1{\times}10^{6}$ / cells of sarcoma 180 twenty four hours before the first injection, the extracts of Agaricus campestris, Lyophyllum decastes, Lyophyllum ulmarium, Armillaria Tabescence and Calvatia exipuliformis respectively showed inhibition ratios of 64.1%, 65.45, 60.-%, 53.0 and 49.3%. These five species were selected for further study, whereas the extracts of Phallus impudicus, Coprinus comatus and Pholiota squarrosa whih showed the inhibition ratios of 31.2%, 33.5% and 19.0% were discontinued.
Temperature Dependence of Magnetization of Amorphous TM70Cr5Si10B15 (TM = Fe, CO, Ni) Alloys
Kyeong-Sup Kim,Seong-Cho Yu,Woo-Young Lim,Wha-Nam Myuong 한국자기학회 1997 Journal of Magnetics Vol.2 No.4
We report the salient features of the magnetic properties of amorphous TM_(70)Cr_5Si_(10)B_(15) (TM=Fe, CO, Ni) alloys. The temperature dependence of magnetization for amorphous ribbons were measured by a SQUID and a VSM from 5 K to 700 K under an external field of 10 kOe. Except TM_(70)Cr_5Si_(10)B_(15) that shows a paramagnetic behaviour, both Fe and Co based amorphous alloys show a typical ferromagnetic thermo-magnetization curves. For these two ferromagnetic alloys, the saturation magnetization in the temperature range from 5 K to about 0.4 Tc can be descrived by the Bloch relation, Ms (T) = Ms(0) [1-BT^(3/2)-CT^(5/2)]. The spin wave stiffness constants and the range of exchange interaction were analyzed from the magnetization behaviour. The variation of the magnetic properties are discussed and compared with the composition of the alloys.