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Agrobacterium tumefaciens를 이용한 amorpha-4,11-dience 합성 유전자의 상추로의 도입
정귀미,송명종,백남인,박영두 경희대학교식량자원개발연구소 2005 硏究論文集 Vol.24 No.-
Amorpha-4,11-diene synthase(ADS) 유전자를 상추에 도입하기 위해 ADS유전자를 pILTAB357에 삽입하여 binary vector pADS를 제작하였다. 상추 형질전환은 자엽 절편체를 pADS 벡터를 운반하는 A. tumefaciens LBA4404에 접종시키고 MS배지에 200 mg·L 세파탁심과 50 mg·L 가나마이신이 첨가된 선발 재분화배지에서 선발하였다. 선발된 신초는 뿌리 유기 배지에서 뿌리를 유기한 후 뿌리가 발생한 신초는 토양으로 이식하여 재배하였다. 형질전환상추에 T-DNA가 전이되었는지를 확인하기 위해 genomic DNA를 이용하여 PCR과 Southern 혼성화 분석을 실시하였다. PCR 분석 결과 9개의 재분화 개체로부터 기대했던 1.6 kb의 산물을 확인할 수 있었으며 Southern 혼성화 분석 역시 각 재분화 개체에 ADS 유전자가 1개 내지 3개의 copy수로 전이된 것을 확인할 수 있었다. 형질전환 상추내의 artemisinin 함량을 GC와 GC/MS 방법으로 분석하였다. Artemisinin 표준물은 retention time이 10분 7초로 나타났으며 이 peak가 artemisinin인지 확인하기 위하여 GC/MS를 측정한 결과 m/z 282가 정확하게 나타나 artemisinin으로 확인되었다. 한편 형질전환 상추와 일반 대조구 상추의 시료도 GC chromatogram에서 artemisinin의 retention time인 10분 7초 부근에서 여러 peak이 겹쳐서 나타났으나 각 peak에 대하여 GC/MS를 측정한 결과 m/z 282을 나타내는 peak는 관측되지 않아 형질전환 상추와 일반 상추에는 artemisinin이 함유되어 있지 않은 것으로 확인되었다. To introduce amorpha-4,11-diene synthase (ADS) gene into lettuce plant, binary vector pADS was constructed by the insertion of an ADS gene to pILTAB357. Cotyledon explants were infected by A. tumefaciens LBA4404 carrying pADS vector to transform lettuce plant with ADS gene. Transformed shoots were selected on selective regeneration medium containing MS salts, 200 mg·L cefotaxim, and 50 mg·L kanamycin. The selected shoots were transferred to rooting medium, and regenerated shoots were transplanted to pots after roots appeared. PCR and Southern hybridization analysis of genomic DNA were performed to confirm expected T-DNA fragments in the transgenic lettuce plants. PCR analysis revealed the presence of the expected 1.6 kb product from the nine regenerants. Southern hybridization analysis also showed that ADS gene was transferred into transgenic lettuce plants with 1~3 copies. Artemisinin in lettuce was identified by GC and GC/MS analysis. Standard artemisinin was found at 10 min 7 sec in the retention time by GC analysis and confirmed m/z 282 by GC/MS. There were several peaks at retention time of both non-transformed and transformed lettuce plants. However, GC/MS analysis showed that peak with m/z 282 was not found in transgenic lettuce plants.
Performance Analysis for Mirrors of 30 cm Cryogenic Space Infrared Telescope
Park, Kwi-Jong,Moon, Bong-Kon,Lee, Dae-Hee,Jeong, Woong-Seob,Nam, Uk-Won,Park, Young-Sik,Pyo, Jeong-Hyun,Han, Won-Yong 한국우주과학회 2012 Journal of Astronomy and Space Sciences Vol.29 No.3
We have designed a 30 cm cryogenic space infrared telescope for astronomical observation. The telescope is designed to observe in the wavelength range of 0.5~2.1 μm, when it is cooled down to 77 K. The result of the preliminary design of the support structure and support method of the mirror of a 30 cm cryogenic space infrared telescope is shown in this paper. As a Cassegrain prescription, the optical system of a 30 cm cryogenic space infrared telescope has a focal ratio of f/3.1 with a 300 mm primary mirror (M-1) and 113 mm secondary mirror (M-2). The material of the whole structure including mirrors is aluminum alloy (Al6061-T6). Flexures that can withstand random vibration were designed, and it was validated through opto-mechanical analysis that both primary and secondary mirrors, which are assembled in the support structure, meet the requirement of root mean square wavefront error <λ/8 for all gravity direction. Additionally, when the M-1 and flexures are assembled by bolts, the effect of thermal stress occurring from a stainless steel bolt when cooled and bolt torque on the M-1 was analyzed.
Kang, Seong-Kwi,Park, Nam-Yong,Cho, Ho-Sung,Shin, Sung-Shik,Kang, Mun-Il,Kim, Sang-Ki,Hyun, Changbaig,Park, In-Chul,Kim, Jong-Tack,Jeong, Cheol,Park, Sung-Hee,Park, Su-Jin,Jeong, Jae-Ho,Kim, You-Jung AAVLD 2006 JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION Vol.18 No.2
<P>The mitotic index is reported to be correlated with recurrence, mean patient survival, and metastasis of canine hemangiopericytoma (CHP). However, to the authors' knowledge, studies investigating the parameters that can predict recurrence or metastasis of CHP with low mitotic index have not been done. To evaluate growth kinetics of CHP with low mitotic index, a retrospective analysis of the proliferative activity by antiproliferative cell nuclear antigen monoclonal antibody and DNA contents by flow cytometry (FCM) was performed with 21 formalin-fixed and paraffin-embedded CHP samples. Of the 21 tumors evaluated by FCM, 6 (26.6%) were aneuploid tumors, and 15 (71.4%) were diploid tumors. There was significant correlation between the PCNA index and ploidy pattern. The diploid group had 39.1 +/- 9.2 PCNA index, whereas the aneuploid group's proliferative cell nuclear antigen (PCNA) index was 63.1 +/- 8.2. The diploid group had mean mitotic index value of 1.140 +/- 0.855, and the aneuploid group had a mean value of 1.067 +/- 0.767. From these results, the CHP samples with low mitotic index were classified into either the aneuploid group with higher PCNA index or the diploid group with lower PCNA index, suggesting that DNA ploidy and proliferative activity may give an indication about malignancy of CHPs with a low mitotic index.</P>
Development of the Earth Observation Camera of MIRIS
Lee, Dae-Hee,Han, Won-Yong,Park, Young-Sik,Park, Sung-Jun,Moon, Bong-Kon,Ree, Chang-Hee,Pyo, Jeong-Hyun,Jeong, Woong-Seob,Nam, Uk-Won,Lee, Duk-Hang,Park, Kwi-Jong,Bae, Soo-Ho,Rhee, Seung-Wu,Park, Jong 한국우주과학회 2011 Journal of Astronomy and Space Sciences Vol.28 No.3
문귀남(Kwi-Nam Moon),박창윤(ChangYun Park) 한국정보과학회 2000 한국정보과학회 학술발표논문집 Vol.27 No.2Ⅲ
인터넷의 급속한 증가는 네트워크상의 트래픽 형성에 중요한 변화를 가져오게 되었다. 통신 성능 향상을 지원하는 과정에서 여러 가지 통신상의 이상 현상들이 발생하게 되었으며, 그로 인한 통신상의 성능 문제에 부딪히게 되었다. 본 논문에서는 이러한 네트워크상의 비정상적인 트래픽의 예 중에서 비순서적 도착(Out-of-Order)에 대해서 실험을 하였으며, 트래픽 컨디셔너라는 개념과 이 개념을 도입한 독립형 구현 모델을 이용해서 네트워크상의 통신 성능을 개선하는 방법에 대해서 설명한다.