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Junji Kurabayashi,Koji Tsumura 제어로봇시스템학회 2009 제어로봇시스템학회 국제학술대회 논문집 Vol.2009 No.8
In this paper, we deal with approximation methods of large-scaled complex systems and derive the condition that the equilibrium point of the error dynamics is exponentially stable. Especially, we propose multi-layered hierarchical model which approximates the large-scaled systems. Our heterogeneous approximation is preferable for preserving the details of a subsystem of our interest and simplifying the other parts of such large-scaled systems. Finally, numerical simulations are demonstrated to show the efficiency.
The CCR4-NOT Complex Is Implicated in the Viability of Aneuploid Yeasts
Tange, Yoshie,Kurabayashi, Atsushi,Goto, Bunshiro,Hoe, Kwang-Lae,Kim, Dong-Uk,Park, Han-Oh,Hayles, Jacqueline,Chikashige, Yuji,Tsutumi, Chihiro,Hiraoka, Yasushi,Yamao, Fumiaki,Nurse, Paul,Niwa, Osami Public Library of Science 2012 PLoS genetics Vol.8 No.6
<P>To identify the genes required to sustain aneuploid viability, we screened a deletion library of non-essential genes in the fission yeast <I>Schizosaccharomyces pombe</I>, in which most types of aneuploidy are eventually lethal to the cell. Aneuploids remain viable for a period of time and can form colonies by reducing the extent of the aneuploidy. We hypothesized that a reduction in colony formation efficiency could be used to screen for gene deletions that compromise aneuploid viability. Deletion mutants were used to measure the effects on the viability of spores derived from triploid meiosis and from a chromosome instability mutant. We found that the CCR4-NOT complex, an evolutionarily conserved general regulator of mRNA turnover, and other related factors, including poly(A)-specific nuclease for mRNA decay, are involved in aneuploid viability. Defective mutations in CCR4-NOT complex components in the distantly related yeast <I>Saccharomyces cerevisiae</I> also affected the viability of spores produced from triploid cells, suggesting that this complex has a conserved role in aneuploids. In addition, our findings suggest that the genes required for homologous recombination repair are important for aneuploid viability.</P><P><B>Author Summary</B></P> <P>Aneuploidy is a major cause of abortive development and is implicated in tumorigenesis in humans. Recent studies revealed that the increased need for protein degradation might account for the detrimental effects of aneuploidy on a cell. Here, we investigated the genetic systems responsible for aneuploid viability. Using a collection of gene deletions in fission yeast, we isolated mutants that affect aneuploid viability. We found that an evolutionarily conserved transcription regulator, the CCR4-NOT complex, and its related factors are required for aneuploid viability, suggesting that regulation of mRNA turnover is required to tolerate aneuploidy. In addition, homologous recombination repair is important for aneuploid viability.</P>
Laser Micro-Machining Using Near-Field Optics
Haseung Chung,Katsuo Kurabayashi,Suman Das 대한기계학회 2008 대한기계학회 춘추학술대회 Vol.2008 No.5
Solid immersion lenses (SIL) facilitate high numerical aperture (NA) and consequent sub-wavelength diffraction limited focusing in near-field optics based systems. Such systems are in commercial and research use for various applications including near-field scanning optical microscopy, ultra-high density magnetooptic data storage and near-field nanolithography. Here, we present a novel manufacturing method using SIL-based near-field optics for laser-induced patterning on silicon wafers. Results of experiments that were conducted at various processing conditions to investigate the effects of varying incident laser power, pulse repetition rate, pulse width, number of pulses and size of SIL on processed feature size and resolution are presented.
Sim, Dong Y.,Kurabayashi, T.,Minami, K.,Esashi, M. 경북대학교 센서기술연구소 1994 센서技術學術大會論文集 Vol.5 No.1
We report the design, fabrication and performance of a silicon microvalve to control precise gas flow. The valve is actuated by pneumatic force for opening and closing, and is assisted by electrostatic force for full shut off. The valve consists of two micromachined components which are bonded together by Au-Si eutectic bonding. One part contains the gas flow inlet and outlet, the other part deflectable silicon actuators which consist of corrugated diaphragms. This actuator is designed to be operated by combination of the pneumatic and electrostatic forces. A flow of 30 sewn at the outlet could be controlled, with the inlet pressure of 0.5 kgf/cm^(2). The leakage of the valve was 0.08 SCCM with the inlet pressure of 0.5 kgf/cm^(2) and the pneumatic air pressure of 0.5 kgf/cm^(2). This valve can potentially be baked for the purpose of precise gas control for sophisticated semiconductor processes.
Preprogrammed, Parallel On-Chip Immunoassay Using System-Level Capillarity Control
Kim, Sung-Jin,Paczesny, Sophie,Takayama, Shuichi,Kurabayashi, Katsuo American Chemical Society 2013 ANALYTICAL CHEMISTRY - Vol.85 No.14
<P>Fully manual use of conventional multiwell plates makes enzyme-linked immunosorbent assay (ELISA)-based immunoassays highly time-consuming and labor-intensive. Here, we present a capillarity-driven on-chip immunoassay that greatly saves time and labor with an inexpensive setup. Our immunoassay process starts with pipetting multiple solutions into multiwells constructed on a microfluidic device chip. Subsequently, capillarity spontaneously transports multiple sample solutions and common reagent solutions into assigned detection channels on the chip in a purely passive and preprogrammed manner. Our device implements capillarity-driven immunoassays involving four sample and six reagent solutions within 30 min by orchestrating the functions of on-chip passive components. Notably, our immunoassay technique reduces the total number of pipetting processes by ∼5 times, as compared to assays on multiwell plates (48 vs 10). This assay technique allows us to quantify the concentrations of C-reactive protein and suppressor of tumorigenicity 2 with a detection limit of 8 and 90 pM, respectively. This device should be useful for sophisticated, parallel biochemical microfluidic processing in point-of-care settings under limited resources.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancham/2013/ancham.2013.85.issue-14/ac401292d/production/images/medium/ac-2013-01292d_0005.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ac401292d'>ACS Electronic Supporting Info</A></P>