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Ko, Young-Joon,Lee, Hyang-Sim,Jeoung, Hye-Young,Heo, Eun-Jeong,Ko, Hyo-Rim,Chang, Byung-Sik,Joo, Hoo-Don,Gerelmaa, U.,Dashzeveg, B.,Tserendorj, S.,Sodnomdarjaa, R.,Park, Jong-Hyeon,Kweon, Chang-Hee,Ch American Society for Microbiology 2010 CLINICAL AND VACCINE IMMUNOLOGY Vol.17 No.1
<B>ABSTRACT</B><P>A blocking enzyme-linked immunosorbent assay (ELISA) with a baculovirus-expressed structural protein was developed for the detection of antibodies to foot-and-mouth disease virus type A. It exhibited 99% specificity with a cutoff of 53% inhibition. Its sensitivity was comparable to the sensitivities of the virus neutralization test and the liquid-phase blocking ELISA, indicating its potential as an alternative assay.</P>
Ko, Moon-Jeong,Choi, Hyo-Sung,Ahn, Joon-Ik,Kim, So-Young,Jeong, Ho-Sang,Chung, Hye-Joo Korea Genome Organization 2008 Genomics & informatics Vol.6 No.3
Recently, obesity has become a worldwide public health concern and the use of anorectic drugs has drastically increased. In this study, sibutramine and phendimetrazine, representative marketed anorectics, were repeatedly administered per os on a daily basis into C57BL/6 mice and the effects of these drugs on food intakes, body weight changes and gene expression profiles were monitored for up to following 7 days. Methamphetamine, which has a potent anorectic effect, was used as a positive control. Anorectic effects were sustained only for two days by phendimetrazine or methamphetamine, but for six days by sibutramine. The modulations of gene expressions in the hypothalamus and the striatum were investigated using microarrays on day 2 and day 7 post-administration, which corresponded to the anorectic period and a return of appetite respectively, for all three drugs tested. Differences in overall gene expression profiles in the stratum on day 2 for sibutramine and phendimetrazine seems to reflect difference between the two in terms of the onsets of drug tolerance. According to microarray findings, the Ankrd26 gene appears to have an important anorectic role, whereas the up-regulation of the olfaction system appeared to be involved in the drug tolerance of anorectics. The microarray data presented in this study demonstrates the usefulness of gene expression analysis for gathering information on the efficacy and safety of anorectic drugs.
Pregnancy Rate of In Vitro Produced Korean Cattle Embryos according to Transport Time Course
Hyo Young Park,Eun Young Kim,Young Hun Kim,Seong Ho Mun,Chang Eon Oh,Young Joon Han,Nam Hyung Kim,Sung Soo Lee,Moon Suck Ko,Key Zung Riu,Se Pill Park 한국동물번식학회 2009 Reproductive & developmental biology Vol.33 No.4
딸기 재배용 2단 베드 시스템에서 휴작기 이용 방울토마토 재배 시 정식 시기에 따른 생육과 생산성 분석
최효길(Hyo Gil Choi),문병용(Byoung Yong Moon),강남준(Nam Jun Kang),고대환(Dae Whan Ko),권준국(Joon Kook Kwon),이재한(Jae Han Lee),박경섭(Kyoung Sub Park) 한국원예학회 2016 원예과학기술지 Vol.34 No.2
본 실험은 딸기 재배 후 휴작기에 후작물로 방울토마토 ‘루비’의 정식시기에 따른 생산성을 결정하기 위하여 수행하였다. 방울토마토는 딸기 전용상토로 충진된 2단 고설 벤치에 2015년 4월 20일, 4월 30일 그리고 5월 10일에 정식되었다. 과일의 수확조사는 7월 31일까지 이루어졌다. 양액농도는 정식초기에 EC 1.2dS·m<SUP>-1</SUP>로 시작하여 점진적으로 농도를 높여 1화방 개화 후에는 EC 2.5dS·m<SUP>-1</SUP>로 공급하였다. 네덜란드의 PBG 양액을 이용하여 공급주기를 하루에 1분 6회로 공급하였다. 정식시기에 따른 방울토마토의 식물체 길이생장은 상단에 비하여 하단에 정식한 것이 유의하게 컸었으며, 일찍 정식한 것이 늦게 정식한 식물체 보다 길이생장이 컸다. 과일의 생산량은 4월 20일 하단베드에 정식한 방울토마토의 식물체당 평균 수확량이 2,954g으로 가장 높았다. 정식 시기나 베드 위치에 따른 과일의 평균무게나 과일의 당도에는 유의한 차이가 없었다. 방울토마토의 총 생산량은 4월 20일에 정식한 것이 4월 30일과 5월 10일에 정식한 방울토마토에 비하여 각각 18%와 34%로 높았다. 우리는 방울토마토를 일찍 정식하였을 때, 생산량이 증가되는 것을 확인하였다. 이 결과는 농민들에게 2단 베드에서 딸기 후작물로 방울토마토의 재배 기간을 선택하는데 도움을 줄 것으로 판단된다 . This study was conducted to determine the yield of cherry tomato (Solanum lycopersicum Mill.) grown at three planting times during the uncultivated period of strawberry. Cherry tomato was planted under condition filled with strawberry dedicated culture medium on a two-story bed with April 20, April 30, and May 10 at 2015. Fruit harvest was completed on July 31. The supply concentration of nutrient solution at the time of transplanting was started as EC 1.2 dS·m<SUP>-1</SUP> and it was gradually increased to EC 2.5 dS·m<SUP>-1</SUP> after blooming of the first flower cluster. Netherlands PBG solution was supplied for one minute six times per day. The heights of cherry tomato plants planted at earlier were significantly greater than those of tomato plants planted later. The heights of cherry tomato plants grown at the bottom of the bed were greater than those grown in the upper bed. The yield of cherry tomatoes planted on April 20 at the bottom of the bed was greatest with an average of 2,954 g of tomatoes per plant. There were no significant differences in the average weight and sugar content of fruit according to planting times and bed position. The yield of cherry tomato plants planted on April 20 was 18% and 34% higher than that of plants planted on April 30 and May 10. We confirmed to increase the yield of the cherry tomato when early plants planted on two-story bed. These results indicate that farmers can choose the best period of producing cherry tomato during the un-cultivated period of strawberry under two-story bed conditions.
Pregnancy Rate of In Vitro Produced Korean Cattle Embryos according to Transport Time Course
Park, Hyo-Young,Kim, Eun-Young,Kim, Young-Hun,Mun, Seong-Ho,Oh, Chang-Eon,Han, Young-Joon,Kim, Nam-Hyung,Lee, Sung-Soo,Ko, Moon-Suck,Riu, Key Zung,Park, Se-Pill The Korean Society of Animal Reproduction 2009 Reproductive & developmental biology Vol.33 No.4
This study was to investigate pregnancy rate of IVM/IVF/IVC Korean cattle (registered in government) embryos according to transport time course. For the production of embryos, oocytes recovered from slaughtered excellent grade cow and highly motile frozen-thawed bull semen (purchased from LIMC, KPN#497) was used. In vitro produced embryos were cultured in CR1aa medium for 8 days and some of them were frozen. The rate of average cleavage (>2-cell) was 83.0% (308/371) and blastocyst rate at day 8 was 34.7% (107/308). Among in vitro produced blastocyst embryos at day 8, most healthy embryos were freshly transferred on production day and some frozen embryos were direct transferred on appropriate day. These embryos were produced in a laboratory, embryo transfer (ET) was planned in 10 areas of the remote island (Jeju) from the laboratory by airplane. Thus, we examined the pregnancy rate in recipient cow according to embryo of transport time course before ET. From embryo transferred 44 recipient cows, overall pregnancy was 40.9% (18/44), these 18 cows were all calved [single, 94% (17/18); twin, 6% (1/18)] and total embryo implantation rate was 26% (19/66). Comparing transport time in the base of 6 hr, pregnancy rate in ET group required less 4 hr (60%, 9/15) was significantly higher than that required more 6 hr (26.3%, 5/19). In direct ET of freezing embryos, the pregnancy rate was 40% (4/10). However, it was difficult to find the meaning of temperature, pH and corpus luteum quality of recipients on comparison of pregnancy rate. When the cell death level of embryos according to storage time in thermos (straw container) before ET was measured by TUNEL staining, apoptotic index was increased with storage time-dependent. These results demonstrated that long distance transfer of IVM/IVF/IVC embryos is possible and the time of embryo transport is very important for the pregnancy rate on field trial.