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Park Se Ra,Ko Kisung,Lim Sohee,Cha So Yeon,정현주,Park Soon Ju,Myung Soon‐Chul,Kim Mi Kyung 한국곤충학회 2020 Entomological Research Vol.50 No.4
The monoclonal antibody (mAb) CO17-1A specifically binds to the tumor associated cell surface glycoprotein GA733 in colorectal cancer cells. Thus, mAb CO17-1A has the potential to act as an immune therapeutic protein against colorectal cancer. Recently, it was shown that the baculovirus insect cell expression system produces anti-colorectal cancer mAb CO17-1A. In this study, the colorectal cancer antibody mAb CO17-1A fused to the endoplasmic reticulum (ER) retention signal sequence (KDEL), and the (mAb CO17-1AK) was expressed in Spodoptera frugiperda Sf9 insect cells. The yield, cell cytotoxicity, and in vitro anti-tumor activity of mAb CO17-1AK were verified. Western blotting was performed to confirm that both heavy and light chains of mAb CO17-1A were expressed in Sf9 insect cells. The insect-derived mAb (mAbI ) CO17-1A was purified using a protein G affinity column. An in vitro wound healing assay was conducted to determine the inhibition activity of mAb CO17-1A during tumor cell migration, showing that mAbI CO17-1AK was effective as mammalian-derived mAb CO17-1A (mAbM CO17-1A). These results suggest that the insect cell expression system can produce and properly assemble mAbs that inhibit tumor cell migration.
Park, Ji-Yeon,Yang, Angel,Park, Jong-Hyouk,Abd El-Aty, A.M.,Oh, Jae-Ho,Do, Jung-Ah,Kwon, Kisung,Shim, Ki-Hoon,Choi, Ok-Ja,Shim, Jae-Han The Korean Society for Applied Biological Chemistr 2012 Applied Biological Chemistry (Appl Biol Chem) Vol.55 No.4
Separation of pesticides and other chemical contaminants from fatty food matrices prior to subsequent steps in the analytical process remains a challenging issue, and much effort has been invested to further enhance this method. The aim of the present study was to develop a simple multi-residue method involving a quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction method for the identification and quantification of 41 pesticide residues in cooked fatty food matrices, including heated soybean oil, roasted sesame, and boiled soybean using gas chromatography-micro/electron capture detector (GC-${\mu}ECD$). The analytes were subsequently confirmed via GC-mass spectrometry (MS). The responses of analytes were linear with excellent correlation coefficients ($r^2$) ranging from 0.993 to 1.000 (calculated from absolute peak areas). For the majority of the tested pesticides, the mean recoveries ranged from 68.5 and 121.4% with relative standard deviations ranging from 0.4 to 18.7%. Instrument limits of detection and quantification ranged from 0.004 to $0.30{\mu}g/kg$ and 0.0125 to $1.00{\mu}g/kg$, respectively. The developed method presented in this study was applied successfully to determine pesticide residue levels in cooked fatty food matrices. None of the samples contained detectable amounts of pesticide residues.
Kisung Park,Soonhwi Hwang,Hwanseok Yang,Chul Hyun,Jai-ick Yoh 한국광학회 2024 Current Optics and Photonics Vol.8 No.2
This study is essential for advancing our knowledge about the interaction between long-range highpower lasers and energetic materials, with a particular emphasis on understanding the response of a 155-mm shell under various surface irradiations, taking into account external factors such as atmospheric disturbances. The analysis addresses known limitations in understanding the use of non-realistic targets and the negligence of ambient conditions. The model employs the three-dimensional level-set method, computer-aided design (CAD)-based target design, and a message-passing interface (MPI) parallelization scheme that enables rapid calculations of the complex chemical reactions of the irradiated high explosives. Important outcomes from interaction modeling include the accurate prediction of the initiation time of ignition, transient pressure, and temperature responses with the location of the initial hot spot within the shell, and the relative magnitude of noise with and without the presence of physical ambient disturbances. The initiation time of combustion was increased by approximately a factor of two with atmospheric disturbance considered, while slower heating of the target resulted in an average temperature rise of approximately 650 K and average pressure increase of approximately 1 GPa compared to the no ambient disturbance condition. The results provide an understanding of the interaction between the high-power laser and energetic target at a long distance in an atmospheric condition.
Park, Da-Young,Lee, Jung-Hwan,So, Yang-Kang,Kim, Young-Kwan,Ko, Kinarm,Park, Sang-Won,Lee, Yong Seok,Han, Yeon Soo,Ko, Kisung Mary Ann Liebert 2011 Hybridoma Vol.30 No.5
<P>The baculovirus-insect cell system is considered a feasible expression system for recombinant glycoprotein production due to its several advantages, including high capacity, flexibility, and glycosylation capability. However, accurate titering of the recombinant baculovirus is required to ensure high expression in insect cells using a commercial and expensive immunoassay titer kit in which the envelope glycoprotein of the Autographa californica multiple nucleopolyhedrovirus (AcMNPV)-type baculovirus is detected by anti-envelope glycoprotein antibody and a secondary antibody conjugated to horseradish peroxidase (HRP). In this study, conditions for the expression of the CO17-1A immunotherapeutic monoclonal antibody (MAb) against colorectal cancer cells in a baculovirus system were optimized without using a commercial titering kit. Several variables were investigated to optimize antibody expression in a baculovirus-insect cell system, including baculovirus passage, volume of the infecting baculovirus inoculum (100, 200, 400, and 800?μL), and the harvest time of insect cells or cell supernatants after virus infection (24, 48, and 72?h). Two different pFastBac vectors carrying the CO17-1A MAb genes with or without the KDEL endoplasmic reticulum (ER) retention motif (Lys-Asp-Glu-Leu) fused to the HC (MAb CO17-1A K and MAb CO17-1A, respectively) were constructed and used to generate baculoviruses. Immunoblot analysis was conducted to confirm expression of MAb CO17-1A K and MAb CO17-1A in baculovirus-infected insect cells. Densitometry analysis of the protein bands was used to quantify the relative expression under different conditions. The highest expression was observed in lysed cells infected with 400?μL of passage 3 baculovirus (P(3) BV) carrying the gene encoding the CO17-1A MAb without KDEL at 72?h after virus infection. These results suggest that the infection conditions, the number of virus passages, baculovirus inoculum volume, and the harvest time can be modified to optimize MAb expression without using a BaculoELISA titer kit in a baculovirus-insect cell system.</P>
빈발 부분그래프 마이닝의 반복 수행에서 중복 연산 제거 기법
박기성(Kisung Park),한용구(Yongkoo Han),이영구(Young-Koo Lee) 한국정보과학회 2012 정보과학회논문지 : 데이타베이스 Vol.39 No.3
빈발 부분그래프는 그래프의 고유한 특성을 나타낼 수 있는 유용한 특징으로 그래프의 분류, 군집화, 인덱싱 등에서 사용된다. 최근에 최소 지지도를 변화시켜 가면서 또는 그래프 DB를 분할하며 반복적으로 빈발 부분 그래프를 마이닝 하는 방법이 사용되고 있다. 이와 같은 마이닝 기법들은 수행 시간이 매우 길며, 반복되는 빈발 부분그래프 마이닝이 전체 시간의 대부분을 차지한다. 본 논문에서는 빈발 부분그래프 마이닝의 반복적 실행을 분석하여, 고비용인 그래프 정규화 연산의 중복 실행에 대하여 논한다. 빈발 부분 그래프 마이닝이 수행될 때마다 같은 그래프에 대한 불필요한 정규화 연산을 방지하기 위하여, 그래프의 정규 형태를 트리 구조로 인덱싱하는 정규 그래프 탐색 트리를 제안한다. 또한 정규 그래프 탐색 트리를 압축하여 유지비용을 줄이는 방안을 함께 제안하다. 대표적인 반복적 마이닝 기법인 모델 기반 탐색 트리를 통한 실험에서 제안하는 기법이 기존의 방법보다 최대 15%까지 수행시간을 단축함을 보인다. Frequent subgraphs represent intrinsic property of graphs and they can be used as significant features for various applications such as classification, clustering, and indexing of a graph DB. In order to select useful features, recent graph mining techniques applies repeated mining of frequent subgraphs either by varying minimum supports or by dividing a graph DB recursively. Such mining techniques suffer from long runtime, and most of the runtime is spent for the repeated mining of frequent subgraphs. In this paper, we discuss redundant execution of expensive canonical graph operations through analyzing the repeated mining of frequent subgraphs. We then propose a novel canonical graph search tree for indexing canonical graphs that can reduce redundant canonical graph operations for the same graph. We also propose a compression technique for the canonical graph search tree in order to reduce the maintenance cost of the tree. In experiment, we show that the proposed technique can reduce runtime by up to 15% compared with the existing model based search tree.