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      • KCI등재

        Phytochemical screening and evaluation of the pharmacological activities of ethanolic extract of Argemone mexicana Linn. aerial parts

        Kishore Kumar Sarkar,Trina Mitra,Rabindra Nath Acharyya,Samir Kumar Sadhu 경희대학교 융합한의과학연구소 2019 Oriental Pharmacy and Experimental Medicine Vol.19 No.1

        The present study was designed to investigate different phytochemical groups present in ethanolic extract of Argemone mexicana aerial parts (EAMA) as well as to assess the analgesic, antidiarrheal, antibacterial, anthelmintic and cytotoxic activities of EAMA. Moreover, peripheral and central analgesic activities were evaluated by acetic acid-induced writhing test, formalin-induced paw licking test, tail immersion test and hot plate test. In vivo castor oil-induced diarrheal model and magnesium sulphate induced diarrheal model in mice were utilized for the assessment of antidiarrheal activity. Again, antibacterial activity was evaluated using disc diffusion assay. Anthelmintic activity was carried out on Paramphistomum cervi (Trematoda). Cytotoxic activity was assessed through brine shrimp lethality bioassay. The extract demonstrated the presence of alkaloids, flavonoids, phenolic compounds, tannins, glycosides and gums in phytochemical screening. In case of acetic acid-induced writhing and formalin induced paw licking test, both lower and higher doses of EAMA showed significant percentage inhibition of writhing as well as paw licking respectively (*P < 0.05, vs. control). EAMA at the doses of 200 mg/kg and 400 mg/kg revealed significant latency response (*P < 0.05, vs. control) in delayed phase in both tail immersion as well as hot plate test. Moreover, significant percentage inhibition (*P < 0.05, vs. control) of diarrhea was exposed by both doses of EAMA in in vivo diarrheal models. In disc diffusion assay, EAMA showed antibacterial activities against both gram positive and gram negative bacterial strains. Again, the extract exhibited anthelmintic and cytotoxic activity in a dose dependent manner.

      • SCIEKCI등재

        Control of Late Leaf Spot of Groundnut (Arachis hypogaea) by Extracts from Non-Host Plant Species

        Kishore, G.Krishna,Pande, S.,Rao, J.Narayana The Korean Society of Plant Pathology 2001 Plant Pathology Journal Vol.17 No.5

        The effects of leaf extracts of 14 different non-host plant species on in vitro conidial germination of Phaeoisariopsis personata, the causal organism of late leaf spot(LLS) of groundnut were evaluated. Aqueous and ethanol leaf extracts of Datura metel, Lawsonia inermis and aqueous leaf extracts of Sphaeranthus indicus at 25%(w/v) concentration completely inhibited the conidial germination of P. personata both at 24h and 48h after incubation. Aqueous leaf extracts of Blumea bifoliata, Eucalyptus globules, Ocimum sanctum and Pongamia pinnata, and ethanol leaf extracts of Azadirachta indica and S. indicus inhibited the conidial germination by >90%. Aqueous and ethanol leaf extract of L. inermis and S. indicus were highly inhibitory to conidial germination up to 1% concentration. Aqueous and ethanol leaf extracts of D. metel and ethanol leaf extract of A. indica were highly inhibitory to P. personata even at 0.01% (100 ppm) concentration. Ethanol leaf extract of A. indica up to $80^{\circ}$, aqueous leaf extracts of D. metel and S. indicus up to $100^{\circ}$, and L. inermis up to $60^{\circ}$, were highly stable and retained their fungitoxic effects. Extract of D. metel was antifungal even after 180 days when it was stored both at room temperature and $4^{\circ}$. Aqueous leaf extract of D. metel at 2% concentration effectively reduced the development of LLS by >60%, under greenhouse conditions both in prophylactic and simultaneous applications. Extracts of D. metel could be a potential economical and an eco-frendly alternative for control of late leaf spot, and its efficacy under field conditions is further being evaluated.

      • KCI등재

        Influence of canopy architecture on photosynthetic parameters and fruit quality of mango in tropical region of India

        Kishore Kundan,Singh H. S.,Nath Vishal,Baig M. J.,Murthy D. Sreenivasa,Acharya G. C.,Behera Suchitra 한국원예학회 2023 Horticulture, Environment, and Biotechnology Vol.64 No.4

        Canopy architecture is one of the most critical components infl uencing photoassimilation, yield and quality of fruit crops; thus, it is logical to optimize suitable canopy form with high light interception effi ciency. In this study, the eff ectiveness of three forms of canopy architecture viz., open centre, Y trellis and espalier were assessed, under high density planting system of mango (833 plants ha − 1 ). Y trellis facilitated better availability of photosynthetic photon fl ux density (PPFD) in upper (793 μmol m − 2 s − 1 ) and lower canopy layers (487 μmol m − 2 s − 1 ) by exhibiting moderate interception of photosynthetic active radiation (PAR). Y-trellis also manifested relatively higher photosynthetic activity (6.07 μmol CO 2 m − 2 s − 1 and 5.24 μmol CO 2 m − 2 s − 1 ), stomatal conductance (0.194 μmol H 2 O m − 2 s − 1 and 0.172 μmol H 2 O m − 2 s − 1 ), carbohydrate and protein content in upper and lower canopy layers. On the other hand, espalier system demonstrated high rate of PAR interception and low photosynthetic activity. Mango canopy with Y-trellis and open centre forms provided better results in terms of fl owering intensity and fruit yield. Fruits from Y-trellis exhibited relatively better colour attributes, sucrose and β-carotene content. Alternatively, soluble solid contents, citric acid, carbohydrate and protein content were unaff ected with the training systems. It was evident from PCA biplots that Y-trellis had close proximity with light intensity, photosynthetic characteristics, fl owering intensity, yield, and fruit quality attributes. Moreover, path coeffi cient analysis indicated that PPFD, iPAR and photosynthetic rate ( Pn ) were the most important predictors for determining fl owering in mango. The fi ndings of the present study demonstrate that optimized canopy architecture is instrumental for eff ective utilization of radiation energy for higher fl owering intensity, yield and fruit quality in mango. Economic assessment of training system indicated that Y-trellis was more feasible system when cultivation was aimed to maximise the profi t with no restriction on investment. However under capital constraint situation open centre system was more profi table.

      • KCI등재

        Evaluation of Peak-Fitting Software for Magnesium Quantification through k0-Instrumental Neutron Activation Analysis

        Kishore B. Dasari,조하나,Radojko Ja cimovi c,박병건,선광민 한국원자력학회 2022 Nuclear Engineering and Technology Vol.54 No.2

        The selection and effective utilization of peak-fitting software for conventional gamma-ray spectrumanalysis is significant for accurate determination of the mass fraction of elements, particularly in complexpeak regions. Majority of the peak-fitting programs can derive similar peak characteristics for singletpeaks, but very few programs can deconvolute multi-peaks in a complex region. The deconvolution ofmulti-peaks requires special peak-fitting functions, such as left and right-skew distributions. In the thisstudy, 843.76 keV (27Mg) peak area from the complex region (840 keVe850 keV) determined andcompared using four different peak-fitting programs, namely, GammaVision, Genie2000, HyperLab, andHyperGam. The 843.76 keV peak interfered with 841.63 keV (152mEu) and 846.81 keV (56Mn). The totalMg concentration was determined through k0-instrumental neutron activation analysis by applying theisotopic interference correction factor 27Al(n,p)27Mg through the simultaneous determination of Alconcentration. HyperLab and HyperGam peak-fitting programs reported consistent peak areas, andresultant concentrations agreed with the certified values of matrix-certified reference materials.

      • KCI등재

        Effect of Chemical Fertilizer-adaptive Variants, Pseudomonas aeruginosa GRC2 and Azotobacter chroococcum AC1,on Macrophomina phaseolina Causing Charcoal Rotof Brassica juncea

        Kishore Kumar Joshi,강선철,Varun Kumar,Ramesh Chand Dubey,Dinesh Kumar Maheshwari,Vivek K. Bajpai 한국환경농학회 2006 한국환경농학회지 Vol.25 No.3

        Pseudomonas aeruginosa GRC2, siderophore-producing strain, inhibited growth of Macrophomina phaseolina in vitro and reduced charcoal rot in seeds of Brassica juncea in field when coated with adaptive strains. P. aeruginosa GRC2 and Azotobacter chroococcum AC1 produced indole-3-acetic acid and solubilized insoluble phosphate. A. chroococcum AC1 fixed nitrogen asymbiotically. Urea and diammonium phosphate- adaptive variant strains of P. aeruginosa and A. chroococcum strongly inhibited M. phaseolina in comparison to parental strains. Bacterization of seeds induced seed germination, seedling growth, and enhanced yield of B. juncea by 10.87% as compared to full doses of urea and diammonium phosphate. Both adaptive strains of chemical fertilizers aggressively colonized roots, showing effectiveness to growth and developments of B. juncea.

      • KCI등재후보

        Expressing Cobb Angle as Linear Measurement in Scoliosis and Its Significance: A Clinical and Geometrical Analysis of Scoliosis

        Kishore Puthezhath 대한척추신경외과학회 2017 Neurospine Vol.14 No.4

        Objective: The aim of this study was to formulate an objective clinical and geometric relationship between Cobb angle and the difference between the lengths of convex and concave sides (convexo-concave vertebral difference) of the structural curve in scoliosis. Is it possible to express Cobb angle in such a way that it could be visualized as a length, especially while planning for surgical correction of scoliosis? Methods: Thirty consecutive patients below the age of 19 years with a scoliosis of Cobb angle more than 10 degrees were included in the study. Convexo-concave vertebral difference of the structural curve was measured. Its relationship with the measure of Cobb angle was studied. Results: Author obtained a significant linear correlation between the convexo-concave vertebral differences and the Cobb angle. Using the formula Y=2d.Sin (X/2) the convexo-concave vertebral difference could be predicted. The difference thus obtained gives a quantitative measure of the maximum length of correction possible in the structural curve. Conclusion: It is possible to express the Cobb angle as a function of linear measurement. The author proposes that this would aid the surgeons to accurately and predictably achieve the desired scoliosis correction.

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