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Kim, Ji Hyun,Park, Saerom,Kim, Hyungsup,Kim, Hyung Joo,Yang, Yung-Hun,Kim, Yong Hwan,Jung, Sang-Kyu,Kan, Eunsung,Lee, Sang Hyun Elsevier 2017 Carbohydrate polymers Vol.157 No.-
<P><B>Abstract</B></P> <P>Alginate/bacterial cellulose nanocomposite beads, with well-controlled size and regular spherical shapes, were prepared in a simple manner by entrapping <I>Gluconacetobacter xylinus</I> in barium alginate hydrogel beads, followed by cultivation of the entrapped cells in culture media with a low sodium ion concentration. The entire surface of the alginate hydrogel beads containing the cells was covered with cellulose fibers (∼30nm) after 36h of cultivation. The cellulose crystallinity index of the alginate/bacterial cellulose beads was 0.7, which was slightly lower than that of bacterial cellulose prepared by cultivating dispersed cells. The water vapor sorption capacity of the alginate/bacterial cellulose beads increased significantly from 0.07 to 38.00 (g/g dry bead) as cultivation time increased. These results clearly indicate that alginate/bacterial cellulose beads have a much higher surface area, crystallinity, and water-holding capacity than alginate beads. The immobilization of lipase on the surface of the nanocomposite beads was also investigated as a potential application of this system. The activity and specific activity of lipase immobilized on alginate/bacterial cellulose beads were 2.6- and 3.8-fold higher, respectively, than that of lipase immobilized on cellulose beads. The alginate/bacterial cellulose nanocomposite beads prepared in this study have several potential applications in the biocatalytic, biomedical, and pharmaceutical fields because of their biocompatibility, biodegradability, high crystallinity, and large surface area.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Alginate/bacterial cellulose (BC) beads with well-controlled size and shape were prepared. </LI> <LI> Alginate/BC beads showed high surface area, high crystallinity, and high water-holding capacity. </LI> <LI> Alginate/BC beads were more favorable than alginate beads for lipase immobilization. </LI> <LI> Alginate/BC beads have many potential applications in biocatalytic, biomedical, and pharmaceutical fields. </LI> </UL> </P>
Kim Hyungsup,Kim Dae-Woong,Seo Moon Hwo,Cho Kwang Soo,Haw Jung Rim The Polymer Society of Korea 2005 Macromolecular Research Vol.13 No.2
We developed an algorithm to simulate the generation of virtual nanowebs using the Monte Carlo method. To evaluate the pore size of the simulated multi-layered nanoweb, an estimation algorithm was developed using a ghost particle having zero volume and mass. The penetration time of the ghost particle through the virtual nanoweb was dependent on the pore size. By using iterative ghost particle penetrations, we obtained reliable data for the evaluation of the pore size and distribution of the virtual nanowebs. The penetration time increased with increasing number of layers and area ratio, whereas it decreased with increasing fiber diameter. Dimensional analysis showed that the penetration time can be expressed as a function of the fiber diameter, area ratio and number of layers.
Mathematical Modeling of Zone Drawing Process
Kim, Hyungsup,Cho, Kwang-Soo,Ji, Byung-Chul The Polymer Society of Korea 2004 Macromolecular Research Vol.12 No.2
To provide guidelines and a basic understanding of static and continuous zone drawing processes, we propose two different mathematical models in terms of the processing conditions and material parameters. Although the models are not finely tuned, because of assumptions made, they are still useful for the analysis of the process and for predicting the processibility.
Inhaled Volatile Molecules-Responsive TRP Channels as Non-Olfactory Receptors
Kim Hyungsup,Kim Minwoo,Jang Yongwoo 한국응용약물학회 2024 Biomolecules & Therapeutics(구 응용약물학회지) Vol.32 No.2
Generally, odorant molecules are detected by olfactory receptors, which are specialized chemoreceptors expressed in olfactory neurons. Besides odorant molecules, certain volatile molecules can be inhaled through the respiratory tract, often leading to pathophysiological changes in the body. These inhaled molecules mediate cellular signaling through the activation of the Ca2+- permeable transient receptor potential (TRP) channels in peripheral tissues. This review provides a comprehensive overview of TRP channels that are involved in the detection and response to volatile molecules, including hazardous substances, anesthetics, plant-derived compounds, and pheromones. The review aims to shed light on the biological mechanisms underlying the sensing of inhaled volatile molecules. Therefore, this review will contribute to a better understanding of the roles of TRP channels in the response to inhaled molecules, providing insights into their implications for human health and disease.
시각 요소의 JND(Just Noticeable Difference)를 고려한 디스플레이 화질의 선호도 평가 방안
김형섭(Hyungsup Kim),서원용(Wonyoung Suh),김인기(Inki Kim),윤명한(Myung Hwan Yun) 한국HCI학회 2008 한국HCI학회 학술대회 Vol.2008 No.2
최근 관련 기술의 발전과 디지털 컨버전스의 가속화로 모바일 제품이 소형화, 다기능화 되어 가고 있다. 이러한 경향에 따라, 다양한 기능을 지원하기 위한 높은 사양의 디스플레이가 요구되고 있으며, 많은 업체들이 고화질, 고해상도 디스플레이의 개발에 경쟁적으로 매진하고 있다. 그러나 사용자의 인지적 특성을 고려하지 않은 고해상도 경쟁은 생산비용만 높이는 결과를 초래할 수 있다. 본 연구는 디스플레이의 설계 요소별 선호도를 분석하고, 사람이 탐지할 수 있는 두 자극 간의 최소한의 차이역(difference threshold)인 JND(Just Noticeable Difference)을 활용하여, 설계 요소의 인지적 특성을 파악하는 것을 목적으로 한다. 이를 위하여 모바일 제품에 주로 사용되는 TFT-LCD를 평가 대상으로, 30 명의 피실험자를 대상으로 JND 측정실험을 수행하였으며, 실험결과를 바탕으로 디스플레이에 대한 주요 설계변수들의 특성을 파악하였다. 이 연구결과는 사용자의 선호도를 고려한 모바일 제품의 디스플레이 설계지침으로 활용될 수 있을 것이다.
Rajesh, Thangamani,Kim, Yong Hyun,Choi, Yong-Keun,Jeon, Jong Min,Kim, Hyun Joong,Park, Sung-Hee,Park, Hyung-Yeon,Choi, Kwon-Young,Kim, Hyungsup,Kim, Hyung Joo,Lee, Sang Hyun,Yang, Yung-Hun Humana Press 2013 Applied biochemistry and biotechnology Vol.170 No.2
<P>Amylases are important industrial enzymes that have been applied widely in the food, detergent, and pulp industries and fermentation processes. In the present study, a gene encoding an alpha-amylase from the genomic DNA library of Paenibacillus sp. was identified and characterized. The amylase gene designated amy1 was shown to consist of 1,980?bp and shared sequence identity towards α-amylase genes from other Bacillus sp. The deduced amino acid sequence for Amy1 indicated 80?% sequence identity with other Bacillus strains. Heterologous expression of recombinant Amy1 in Escherichia coli BL21(DE3) facilitated the recovery of this protein in soluble form. Enzyme kinetic data revealed Amy1 to have a K m of 23.83?mg/mL and K cat of 48.74?min(-1) and K cat /K m of 2?min(-1)?mg(-1)?mL(-1) for starch. The activity of this protein was found to be enhanced by Mn(2+), and furthermore, Amy1 remained active at a broad pH range (4-10) and temperature (30-90?C). The ability of Amy1 to act on food waste under broad temperature and pH conditions, together with its ability to produce simple sugars, shows many advantages for further application in the food industry.</P>