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Chu, Ki-Back,Lee, Hae-Ahm,Moon, Eun-Kyung,Quan, Fu-Shi Elsevier Scientific Pub.Co 2019 Veterinary parasitology Vol. No.
<P><B>Abstract</B></P> <P>Maternal antibody transmission via placenta and breastmilk are known to confer protection in infants. In this study, we investigated the maternal immunity transmission in pups delivered by rats infected with <I>Trichinella spiralis</I> and assessed the resulting resistance against subsequent parasitic infection. Our results revealed that parasite-specific IgG, IgG1 and IgG2a antibodies were present in pups prior to breastmilk ingestion (pre-milk), in which IgG and IgG1 antibodies persisted until week 8 after birth while parasite-specific IgG2a antibodies only lasted until week 4. After weaning on week 3, pups delivered by <I>T. spiralis</I>-infected dam and subsequently challenge-infected (immune-challenge) were found to possess higher mucosal IgG antibodies than control groups, whereas mucosal IgA levels were not significantly different across all groups. <I>T. spiralis</I> excretory-secretory antigen was discovered to react with pup sera until week 8, correlating with the resistance against parasitic infection which is represented by lessened worm burden. Upon <I>T. spiralis</I> infection at weeks 3 and 8, lower levels of eosinophil responses were detected in immune-challenge pups compared to naïve-challenge pups, indicating correlates of resistances in which ADCC may be involved. Findings from the present study demonstrate that resistances against <I>T. spiralis</I> infection in pups can be acquired by maternally-derived IgG, IgG1 and IgG2a antibody transmission through the placenta and breastmilk from <I>T. spiralis</I>-infected dam, which lasts until week 8.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>Trichinella spiralis</I>-specific antibodies from dams confer resistance in pups. </LI> <LI> Resistance against <I>T. spiralis</I> in pups wane upon cessation of breastmilk ingestion. </LI> <LI> Maternal antibodies persist in pups up to at least 8 weeks post-birth. </LI> <LI> Transmission of different antibody subclasses to pups involve differing routes. </LI> </UL> </P>
강해지,Ki-Back Chu,Su-Hwa Lee,김민주,박현우,Hui Jin,Fu-Shi Quan 대한기생충학ㆍ열대의학회 2019 The Korean Journal of Parasitology Vol.57 No.5
Toxoplasma gondii can infect humans worldwide, causing serious diseases in pregnant women and immunocompromised individuals. T. gondii rhoptry protein 13 (ROP13) is known as one of the key proteins involved in host cell invasion. In this study, we generated virus-like particles (VLPs) vaccine expressing T. gondii rhoptry ROP13 and investigated VLPs vaccine efficacy in mice. Mice immunized with ROP13 VLPs vaccine elicited significantly higher levels of T. gondii-specific IgG, IgG1, IgG2a, and IgA antibody responses following boost immunization and challenge infection, whereas antibody inductions were insignificant upon prime immunization. Differing immunization routes resulted in differing antibody induction, as intranasal immunization (IN) induced greater antibody responses than intramuscular immunization (IM) after boost and challenge infection. IN immunization induced significantly higher levels of IgG and IgA antibody responses from feces, antibody-secreting cells (ASCs), CD4+ T, CD8+ T cells and germinal center B cell responses in the spleen compared to IM immunization. Compared to IM immunization, IN immunization resulted in significantly reduced cyst counts in the brain as well as lesser body weight loss, which contributed to better protection. All of the mice immunized through either route survived, whereas all naïve control mice perished. These results indicate that the ROP13 VLPs vaccine could be a potential vaccine candidate against T. gondii infection.
Dong-Hun Lee,Ki-Back Chu,Hae-Ji Kang,Su-Hwa Lee,Fu Shi Quan 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.1
Extensive use of antibiotics has caused the microbial resistance to rise drastically within the last few decades, and new approaches are therefore needed to develop effective antibacterial substances. In this study, we identified peptide in the hemolymph of Hermetia illucens larvae using reverse-phase chromatography, HPLC and Nano-LC-ESI-MS/MS system. We investigated the antibacterial effect of HP/F9 peptides against Klebsiella pneumoniae in vitro and in vivo. The peptide effectively inhibited the growth of K. pneumoniae in vitro and completely removed K. pneumoniae from the lungs of mice. Importantly, peptides (22,000 Da, HP/F9) successfully reduced lung inflammation upon K. pneumoniae infection. These results indicate that the HP/F9 peptide from H. illucens larva can effectively protect the mouse from K. pneumoniae infection. HP/F9 could be a new candidate for the development of effective antibacterial substance.
Parasite Infiltration and Apoptosis in Spleen upon Toxoplasma gondii Infection
Su-Hwa Lee,Ki-Back Chu,Fu-Shi Quan 대한기생충학ㆍ열대의학회 2019 The Korean Journal of Parasitology Vol.57 No.5
Toxoplasma gondii infection induces parasite infiltration and apoptosis in the spleen. However, dose-dependent parasite infiltration, apoptosis, body weight alternations and survival in mice remain largely unknown. In this study, mice were intraperitoneally infected with 10, 30 or 100 tachyzoites of T. gondii, respectively. Parasite infiltration and apoptosis in the spleen were analyzed on days 3, 7, and 9 post-infection by immunohistochemistry and flow cytometry. Significantly higher levels of T. gondii infiltration and apoptosis in the spleen were found in 30 and 100 tachyzoites infected mice compared to 10 tachyzoites infected mice on days 7 and 9 post-infection. Although 30 and 100 tachyzoites infected mice showed significant body weight loss compared to 10 tachyzoites infected mice, all of the 100, 30, and 10 tachyzoites infected mice died by days 12, 15, and 17, each respectively. Interestingly, T. gondii infiltration in 10 tachyzoites infected mice were limited to capsule area of the spleen on day 9 post-infection. Several areas of parasite infiltrations were found in the 30 tachyzoites infected mice, where noticeable levels of splenic capsule de-adhesion occurred. These results indicated that parasite infiltration and apoptosis in the spleen, as well as body weight loss (survival) are closely correlated with infection dosage. The level of T. gondii infiltration and apoptosis in the spleen and splenic de-adhesion were dependent on the parasite dose.
Previous Infection with Plasmodium berghei Confers Resistance to Toxoplasma gondii Infection in Mice
Dong-Hun Lee,Ki-Back Chu,강해지,Su-Hwa Lee,전복실 대한기생충학ㆍ열대의학회 2019 The Korean Journal of Parasitology Vol.57 No.2
Both Plasmodium spp. and Toxoplasma gondii are important apicomplexan parasites, which infect humans worldwide. Genetic analyses have revealed that 33% of amino acid sequences of inner membrane complex from the ma- laria parasite Plasmodium berghei is similar to that of Toxoplasma gondii. Inner membrane complex is known to be in- volved in cell invasion and replication. In this study, we investigated the resistance against T. gondii (ME49) infection in- duced by previously infected P. berghei (ANKA) in mice. Levels of T. gondii-specific IgG, IgG1, IgG2a, and IgG2b antibody responses, CD4+ and CD8+ T cell populations were found higher in the mice infected with P. berghei (ANKA) and chal- lenged with T. gondii (ME49) compared to that in control mice infected with T. gondii alone (ME49). P. berghei (ANKA) + T. gondii (ME49) group showed significantly reduced the number and size of T. gondii (ME49) cysts in the brains of mice, re- sulting in lower body weight loss compared to ME49 control group. These results indicate that previous exposure to P. berghei (ANKA) induce resistance to subsequent T. gondii (ME49) infection.
Eun-Kyung Moon,So-Min Park,Ki-Back Chu,Fu-Shi Quan,Hyun-Hee Kong 대한기생충학열대의학회 2021 The Korean Journal of Parasitology Vol.59 No.1
Legionella pneumophila is an opportunistic pathogen that survives and proliferates within protists such as Acanthamoeba spp. in environment. However, intracellular pathogenic endosymbiosis and its implications within Acanthamoeba spp. remain poorly understood. In this study, RNA sequencing analysis was used to investigate transcriptional changes in A. castellanii in response to L. pneumophila infection. Based on RNA sequencing data, we identified 1,211 upregulated genes and 1,131 downregulated genes in A. castellanii infected with L. pneumophila for 12 hr. After 24 hr, 1,321 upregulated genes and 1,379 downregulated genes were identified. Gene ontology (GO) analysis revealed that L. pneumophila endosymbiosis enhanced hydrolase activity, catalytic activity, and DNA binding while reducing oxidoreductase activity in the molecular function (MF) domain. In particular, multiple genes associated with the GO term ‘integral component of membrane’ were downregulated during endosymbiosis. The endosymbiont also induced differential expression of various methyltransferases and acetyltransferases in A. castellanii. Findings herein are may significantly contribute to understanding endosymbiosis of L. pneumophila within A. castellanii.