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RISS 인기검색어
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- 저자 : Khan Abdul L. (검색결과 4 건)
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Evaluation of reference genes for qRT-PCR studies in the colchicine producing Gloriosa superba L.
Johnson Nekha,Rodriguez Diaz Diana,Ganapathy Sivakumar,Bass John S.,Kutchan Toni M.,Khan Abdul L.,Flavier Albert B. 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.4
The flame lily, Gloriosa superba L., is one of the two primary sources of the anti-inflammatory drug, colchicine. Previous studies have shown that a higher level of colchicine production occurs in the rhizomes than in leaves and roots. Earlier precursor feeding and transcriptome analysis of G. superba have provided a putative pathway and candidate genes involved in colchicine biosynthesis. Comparative analysis of expression levels of candidate pathway genes in different tissues of G. superba using quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) can reveal highly expressed genes in the rhizome compared to other tissues which could suggest roles of the gene products in colchicine biosynthesis. Normalization is an important step in effectively analyzing differential gene expression by qRT-PCR with broader applications. The current study selected candidate reference genes from the transcriptome datasets and analyzed them to determine the most stable genes for normalization of colchicine biosynthesis-related genes. Using RefFinder, one stable reference gene, UBC22, was selected to normalize gene expression levels of candidate methyltransferase (MT) genes in the leaves, roots, and rhizomes of G. superba. With UBC22 as reference gene, the methyltransferases, GsOMT1, GsOMT3, and GsOMT4 showed significantly higher expression levels in the rhizome of G. superba, while MT31794 was more highly expressed in the roots. In conclusion, the current results showed a viable reference gene expression analysis system that could help elucidate colchicine biosynthesis and its exploitation for increased production of the drug in G. superba.
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<i>Exophiala</i> sp. LHL08 reprograms <i>Cucumis sativus</i> to higher growth under abiotic stresses
Khan, Abdul L.,Hamayun, Muhammad,Ahmad, Nadeem,Waqas, Muhammad,Kang, Sang‐,Mo,Kim, Yoon‐,Ha,Lee, In‐,Jung Blackwell Publishing Ltd 2011 Physiologia Plantarum Vol.143 No.4
<P>Endophytic fungi are potential sources of secondary metabolites; however, they are little known for phytohormones secretion and amelioration of plant growth under abiotic stresses. We isolated a novel endophyte from the roots of <I>Cucumis sativus</I> and identified it as a strain of <I>Exophiala</I> sp. by sequencing internal transcribed spacer/large subunit rDNA and phylogenetic analysis. Prior to identification, culture filtrate (CF) of <I>Exophiala</I> sp. has shown significant growth promotion of Waito‐C [a gibberellins (GAs)‐deficient mutant cultivar] and Dongjin‐byeo (normal GAs biosynthesis cultivar) rice seedlings. CF analysis of <I>Exophiala</I> sp. showed the presence of physiologically active GAs (GA<SUB>1</SUB>, GA<SUB>3</SUB>, GA<SUB>4</SUB> and GA<SUB>7</SUB>) and inactive GAs (GA<SUB>5</SUB>, GA<SUB>8</SUB>, GA<SUB>9</SUB>, GA<SUB>12</SUB> and GA<SUB>20</SUB>). <I>Exophiala</I> sp. had higher GAs in its CF than wild‐type strain of <I>Gibberella fujikuroi</I> except GA<SUB>3</SUB>. Influence of <I>Exophiala</I> sp. was assessed on cucumber plant's growth and endogenous abscisic acid (ABA), salicylic acid (SA) and bioactive GAs under salinity and drought stresses. <I>Exophiala</I> sp.‐treated plants have shown significantly higher growth and rescued the host plants from stress promulgated water deficit, osmotic and cellular damage. The altered levels of stress‐responsive ABA showed low level of stress confined to endophyte‐applied plants than control. Elevated levels of SA and bioactive GAs (GA<SUB>3</SUB> and GA<SUB>4</SUB>) in endophyte‐associated plants suggest stress‐modulating response toward salinity and drought. In conclusion, symbiotic relations between <I>Exophiala</I> and cucumber have reprogrammed the host plant growth under abiotic stresses, thus indicating a possible threshold role of endophytic fungi in stress alleviation. This study could be extended for improving agricultural productivity under extreme environmental conditions.</P>
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Harith Jameel Mahdi,Nurzalina Abdul Karim Khan,Mohd Zaini Bin Asmawi,Roziahanim Mahmud,Vikneswaran A/L Murugaiyah 한국한의학연구원 2018 Integrative Medicine Research Vol.7 No.1
Background: The medicinal uses of plants are in many cases based exclusively on traditional knowledge without enough scientific evidences. Different parts of Moringa oleifera were traditionally used for the treatment of wide variety of ailments including arthritis and joints pain. The present study had been designed to evaluate the anti-arthritic and anti-nociceptive activities of ethanol extract of Moringa leaves, this being the most abundant plant part suitable for commercial mass production of botanical medicinal products. Methods: Complete Freund's adjuvant (CFA)-induced arthritis in rats was used as disease model. CFA-induced inflammatory paw edema, body weight, arthritic index, X-ray radiography, hematological parameters, and walk track and locomotion analysis were all evaluated for the assessment of disease progression. In addition to that, anti-nociceptive activity was examined at different dose levels in both normal and arthritic-induced rats using Eddy's hot plate and tail flick thermal analgesia. Results: The analysis of various arthritic assessment parameters used in this study revealed that Moringa extract has a considerable effect in preventing development or ameliorate arthritis disease severity. Moreover, the ethanol extract of Moringa leaves revealed significant anti-nociceptive activity at in both normal and CFA-induced arthritis rats in a dose-dependent manner. Conclusion: Ethanol extract of Moringa leaves appears to be a really promising as analgesic and arthritis medication, but a larger and more detailed preclinical and clinical studies especially in human is highly recommended.
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