Rapid and Sensitive Detection of Salmonella in Chickens Using Loop-Mediated Isothermal Amplification Combined with a Lateral Flow Dipstick

( Zhi-ke Liu ),( Qiu-yu Zhang ),( Ning-ning Yang ),( Ming-guo Xu ),( Jin-feng Xu ),( Ming-long Jing ),( Wen-xing Wu ),( Ya-dong Lu ),( Feng Shi ),( Chuang-fu Chen ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.3

Salmonellosis is a highly contagious bacterial disease that threatens both human and poultry health. Tests that can detect Salmonella in the field are urgently required to facilitate disease control and for epidemiological investigations. Here, we combined loop-mediated isothermal amplification (LAMP) with a chromatographic lateral flow dipstick (LFD) to rapidly and accurately detect Salmonella. LAMP primers were designed to target the Salmonella invA gene. LAMP conditions were optimized by adjusting the ratio of inner to outer primers, MgSO₄ concentration, dNTP mix concentration, amplification temperature, and amplification time. We evaluated the specificity of our novel LAMP-LFD method using six Salmonella species and six related non-Salmonella strains. All six of the Salmonella strains, but none of the non-Salmonella strains, were amplified. LAMP-LFD was sensitive enough to detect concentrations of Salmonella enterica subsp. enterica serovar Pullorum genomic DNA as low as 89 fg/μl, which is 1,000 times more sensitive than conventional PCR. When artificially contaminated feed samples were analyzed, LAMP-LFD was also more sensitive than PCR. Finally, LAMP-LFD gave no false positives across 350 chicken anal swabs. Therefore, our novel LAMP-LFD assay was highly sensitive, specific, convenient, and fast, making it a valuable tool for the early diagnosis and monitoring of Salmonella infection in chickens.

Depth Distributions of Bi+ Ions Implanted into Ni, Si and SiO₂, Films

Ke-Ming Wang,Feng Chen,Xue-Lin Wang,Jian-Hua Zhang,Xiang-Dong Liu 한국진공학회(ASCT) 2002 Journal of Korean Vacuum Science & Technology Vol.6 No.1

Ni, Si and SiO₂ films were implanted by 350 keV Bi^+ ions at room temperature with fluences of 1×10^(16) and 2×10^(16) ions/㎤. The depth distributions of implanted Bi^+ ions in Ni, Si and SiO₂ films were by investigated by Rutherford backscattering. The results show that the depth distributions of implanted Bi^+ ions into Ni, Si and SiO₂ films have obeyed nearly Gaussian distributions. The maximum difference between experimental and calculated values is less than 18 % for mean projected range. Experimental range straggling deviated significantly from calculated value. The possible reasons are discussed.

Bibliometric analysis of researches on traditional Chinese medicine for coronavirus disease 2019 (COVID-19)

Ke-Lu Yang,Xin-Yao Jin,Ya Gao,Jin Xie,Ming Liu,Junhua Zhang,Jin-Hui Tian 한국한의학연구원 2020 Integrative Medicine Research Vol.9 No.3

Background: The coronavirus disease 2019 (COVID-19) has caused a worldwide pandemic, and traditional Chinese medicine (TCM) has played an important role in response. We aimed to analyze the published literature on TCM for COVID-19, and provide reference for later research. Methods: This study searched the CBM, CNKI, PubMed, and EMBASE from its establishment to March 11, 2020. VOSviewer 1.6.11 and gCLUTO 2.0 software were used to visually analyze the included studies. Results: A total of 309 studies were included, including 61 journals, 1441 authors, 277 institutions, and 27 provinces. Research collaborations among regions were among those close in geographical distance. The collaborations of institutions and authors were more likely to be restricted to the same region. Among the authors with frequency greater than two (65 authors), only 19 authors had connection with others. More than 70% (358/491) of keywords were only presented once, and 20 keywords were shown more than 10 times. Five research topics were identified: Data mining method based analysis on the medication law of Chinese medicine in prevention and management of COVID-19; exploration of active compounds of Chinese medicine for COVID-19 treatment based on network pharmacology and molecular docking; expert consensus and interpretation of COVID-19 treatment; research on the etiology and pathogenesis of COVID-19; and clinical research of TCM for COVID-19 treatment. Conclusion: The research hotspots were scattered, and the collaboration between authors and institutions needed to be further strengthened. To improve the quality and efficiency of research output, the integration of scientific research and resources, as well as scientific collaboration are needed.

A new species of gall midge (Diptera: Cecidomyiidae) damaging flower buds of goji berry Lycium barbarum (Solanaceae)

Ke-Long Jiao,Xiao-Yi Zhou,Hai-Li Qiao,Hao Wang,Jun Chen,Bai-Ming Liu,Wen-Jun Bu,Peter Kolesik 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.4

Larvae of a previously undescribed gall midge species have been feeding within malformed flower buds of goji berry Lycium barbarum L. (Solanaceae) in China, preventing fruit development and causing damage to the fruit industry. The new species is named Gephyraulus lycantha Jiao & Kolesik, its morphology is described, the COI mitochondrial gene segment is sequenced, and the biology is outlined. In order to taxonomically accommodate the new gall midge, the scope of the genus Gephyraulus is broadened and now encompasses also species that lack dorso-apical sensoria on the female cercus.

Expression of E. coli Phosphofructokinase Gene in an Autotrophic Bacterium Acidithiobacillus thiooxidans

( Tian Ke Li ),( Jian Qun Lin ),( Xiang Mei Liu ),( Ying Liu ),( Chang Kai Zhang ),( Wang Ming Yan ) 한국미생물생명공학회 2004 Journal of microbiology and biotechnology Vol.14 No.1

High performance removal of methyl mercaptan on metal modified activated carbon

Qiang Liu,Ming Ke,Pei Yu,Feng Liu,Haiqiang Hu,Changchun Li 한국화학공학회 2018 Korean Journal of Chemical Engineering Vol.35 No.1

A series of coconut shell activated carbon catalysts, modified by metal oxides, were prepared by an ultrasound- assisted incipient wetness method for the removal of methyl mercaptan (CH3SH). The catalysts were investigated using XRD, BET, XPS, TEM and TA.The results showed that the catalyst combined with 2wt% Fe loading and iron (Fe) : copper (Cu) (mole ratio) 10 : 3, and calcination at 300 oC had a superior removal efficiency. The high activity could be attributed to the generation of highly dispersed Fe-Cu nanocomposites. The results revealed that calcination temperature not only influenced the chemical states and nanocomposite size of iron and copper, but also affected the pore structures of the catalysts. Compared with Fe/AC, the interaction between the iron and copper oxides resulted in smaller nanoparticles and high dispersion for Fe-Cu/AC. Product analysis results suggested dimethyl disulfide, metal methanesulfonates and methyl thiolates were the oxidation products which adsorbed on the activated carbon.

Estimation of the net energy requirement for maintenance in broilers

Wei Liu,Chang Hua Lin,Zheng Ke Wu,Guo Hua Liu,Hai Jie Yan,Hua Ming Yang,Hui Yi Cai 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.6

Objective: The net energy requirement for the maintenance (NEm) of broilers was determined using regression models by the indirect calorimetry method (ICM) or the comparative slaughter method (CSM). Methods: A 2×4 factorial arrangement of treatments including the evaluation method (ICM or CSM) and feed intake (25%, 50%, 75%, or 100% of ad libitum recommended) was employed in this experiment. In the ICM, 96 male Arbor Acres (AA) birds aged d 15 were used with 4 birds per replicate and 6 replicates in each treatment. In the CSM, 116 male AA birds aged d 15 were used. Among these 116 birds, 20 were selected as for initial data and 96 were assigned to 4 treatments with 6 replicate cages and 4 birds each. The linear regression between retained energy (RE) and metabolizable energy intake (MEI) or the logarithmic regression between heat production (HP) and MEI were used to calculate the metabolizable or net energy requirement for maintenance (MEm) or NEm, respectively. Results: The evaluation method did not detect any differences in the metabolizable energy (ME), net energy (NE), and NE:ME of diet, and in the MEI, HP, and RE of broilers. The MEI, HP, and RE of broilers decreased (p<0.01) as the feed intake decreased. No evaluation method× feed intake interaction was observed on these parameters. The MEm and NEm estimated from the linear relationship were 594 and 386 kJ/kg of body weight (BW)0.75/d in the ICM, and 618 and 404 kJ/kg of BW0.75/d in the CSM, respectively. The MEm and NEm estimated by logarithmic regression were 607 and 448 kJ/kg of BW0.75/d in the ICM, and were 619 and 462 kJ/kg of BW0.75/d in the CSM, respectively. Conclusion: The NEm values obtained in this study provide references for estimating the NE values of broiler diets.

ppGalNAc T1 as a Potential Novel Marker for Human Bladder Cancer

Ding, Ming-Xia,Wang, Hai-Feng,Wang, Jian-Song,Zhan, Hui,Zuo, Yi-Gang,Yang, De-Lin,Liu, Jing-Yu,Wang, Wei,Ke, Chang-Xing,Yan, Ru-Ping Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.11

Objectives: To investigate the effect of glycopeptide-preferring polypeptide GalNAc transferase 1 (ppGalNAc T1 ) targeted RNA interference (RNAi) on the growth and migration of human bladder carcinoma EJ cells in vitro and in vivo. Methods: DNA microarray assays were performed to determine ppGalNAc Ts(ppGalNAc T1-9) expression in human bladder cancer and normal bladder tissues. We transfected the EJ bladder cancer cell line with well-designed ppGalNAc T1 siRNA. Boyden chamber and Wound healing assays were used to investigate changes of shppGalNAc T1-EJ cell migration. Proliferation of shppGalNAc T1-EJ cells in vitro was assessed using [3H]-thymidine incorporation assay and soft agar colony formation assays. Subcutaneous bladder tumors in BALB/c nude mice were induced by inoculation of shppGalNAc T1-EJ cells and after inoculation diameters of tumors were measured every 5 days to determine gross tumor volumes. Results: ppGalNAc T1 mRNA in bladder cancer tissues was 11.2-fold higher than in normal bladder tissues. When ppGalNAc T1 expression in EJ cells was knocked down through transfection by pSUPER-shppGalNAc T1 vector, markedly reduced incorporation of [3H]-thymidine into DNA of EJ cells was observed at all time points compared with the empty vector transfected control cells. However, ppGalNAc T1 knockdown did not significantly inhibited cell migration (only 12.3%). Silenced ppGalNAc T1 expression significantly inhibited subcutaneous tumor growth compared with the control groups injected with empty vector transfected control cells. At the end of observation course (40 days), the inhibitory rate of cancerous growth for ppGalNAc T1 knockdown was 52.5%. Conclusion: ppGalNAc T1 might be a potential novel marker for human bladder cancer. Although ppGalNAc T1 knockdown caused no remarkable change in cell migration, silenced expression significantly inhibited proliferation and tumor growth of the bladder cancer EJ cell line.

New Control Strategy for Three-Phase Grid-Connected LCL Inverters without a Phase-Locked Loop

Lin Zhou,Ming Yang,Qiang Liu,Ke Guo 전력전자학회 2013 JOURNAL OF POWER ELECTRONICS Vol.13 No.3

The three-phase synchronous reference frame phase-locked loop (SRF-PLL) is widely used for synchronization applications in power systems. In this paper, a new control strategy for three-phase grid-connected LCL inverters without a PLL is presented. According to the new strategy, a current reference can be generated by using the instantaneous power control scheme and the proposed positive-sequence voltage detector. Through theoretical analysis, it is indicated that a high-quality grid current can be produced by introducing the new control strategy. In addition, a kind of independent control for reactive power can be achieved under unbalanced and distorted grid conditions. Finally, the excellent performance of the proposed control strategy is validated by means of simulation and experimental results.

Preparation and properties of bio-based intumescent flame retardant containing chitosan functionalized ammonium polyphosphate for polyurethane

Shang-Hao Liu,Chen-Feng Kuan,Cing–Yu Ke,Ming-Yuan Shen,Chin-Lung Chiang 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.127 No.-

In this study, chitosan (CS) from fish waste was used to prepare bio-based environmentally friendly flameretardants. After the hydroxyl group (OH) of CS and the ammonium group (NH4 + ) of ammoniumpolyphosphate (APP) underwent reactions, they were filtered and dried to obtain CS-APP. The aminogroup of CS-APP then reacted with the epoxy group of 4,40-methylenebis(N,N-diglycidylaniline) (NDY)to form CS-APP-NDY. Isophorone diisocyanate, polyol, and 3-aminopropyltriethoxysilane reacted to formsilicon polyurethane. CS-APP-NDY was then mixed with Si-PU to prepare polymer composites. To determinethe structure, thermal properties, flame retardancy, and mechanical properties of the composites,Fourier-transform infrared spectroscopy, thermogravimetric analysis, limiting oxygen index (LOI), conecalorimetry, UL-94, thermal analysis-FTIR (TA-FTIR) spectroscopy, universal machine testing, scanningelectron microscopy, X-ray photoelectron spectroscopy, and Raman spectroscopy were performed. TheTGA results revealed that, after the addition of CS-APP-NDY, char yield increased from 0.5 wt% to 25.8wt%, and the thermal stability of pristine PU also improved. In addition, the LOI and UL-94 results indicatedthat, after the addition of CS-APP-NDY, the LOI increased from 18.2% to 26.3%, and the UL-94 levelimproved from ‘‘Fail” to V-1. Overall, these results indicated that the addition of CS-APP-NDY to pristinePU increased its flame-retarding performance.