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Kazuhisa Nakayama,Kim, Won-Sin,Seiji Torii,Masahiro Hosaka,Tsutomu Nakagawa,Jo Ikemizu,Tadashi Baba,Kazuo Murakami 원광대학교기초자연과학연구소 1992 基礎科學硏究誌 Vol.11 No.3
We used the polymerase chain reaction to identify a mouse testis cDNA that represented another member of a growing class of mammalian endoproteases involved in the processing of precursor proteins. This cDNA encoded a 655-residue protein, designated PC4, containing a bacterial subtilisin-like catalytic domain closely related to those of the recently characterized precursor-processing endoproteases, furin, PC1/PC3, PC2, and Kex2. Within this domain, the amino acid sequence of PC4 was 70, 58, 55, and 45% identical with those of mouse furin, mouse PC1/PC3, mouse PC2, and yeast Kex2, respectively. Northern blot analysis indicated that the PC4 mRNA was detectable only in the testes after the 20th day of postnatal development. Moreover, this message was mainly expressed in the round spermatids. These data suggest that PC4 represents a prime candidate for a precursor-processing endoprotease in the testicular germ cells and that its gene expression is regulated during spermatogenesis.
Kazuhisa, Nakayama,Toshio, Watanabe,Tsutomu, Nakagawa,Kim, Won Sin,Masami, Nagahama,Masahiro, Hosaka,Kiyotaka, Hatsuzawa,Kiyomi, Kondoh-Hashiba,Kazuo, Murakami 圓光大學校 基礎自然科學硏究所 1993 基礎科學硏究誌 Vol.12 No.2
Many peptide hormones and neuropeptides are produced from larger, inactive precursors though endoproteolysis at sites usually marked by paired basic residues (primarily Lys-Arg and Arg-Arg), or occasionally by a monobasic residue (primarily Arg). Based upon data concerning processing of prorenin and its mutants around the native Lys-Arg cleavage site expressed in mouse pituitary AtT-20 cells, we present the following sequence rules that govern mono-arginyl cleavages: (a) a basic residue at the fourth (position-4) or the sixth(position-6) residue upstream of the cleavage site is required, (b) at position - 4, Arg ; more favorable than Lys, and (c) at position I, a hydrophobic aliphatic residue is not suitable. These rules are compatible with those proposed by comparison of precursor sequences around mono-arginyl cleavage sites. We also provide evidence that precursor cleavages at mono-arginly and dibasic sites can be catalyzed by the same Kex2-like processing endoprotease, PC1/PC3.
Development of a WLAN Based Monitoring System for Group Activity Measurement in Real-Time
Tsunoda, Hiroshi,Nakayama, Hidehisa,Ohta, Kohei,Suzuki, Akihiro,Nishiyama, Hiroki,Nagatomi, Ryoichi,Hashimoto, Kazuo,Waizumi, Yuji,Keeni, Glenn Mansfield,Nemoto, Yoshiaki The Korea Institute of Information and Commucation 2011 Journal of communications and networks Vol.13 No.2
In recent years, there has been a rise in epidemiological evidence suggesting the health benefits of a physically active lifestyle. However, it is not always easy for individuals to personally recognize the optimal conditions for exercise and physical activity. Wearable acceleration-based pedometers have become widely used in estimating the amount of physical activity, and to a limited extent, providing information regarding exercise intensity, but they have never been used to assess adaptation to exercise. In order to realize simultaneous activity monitoring for multiple users exercising outdoors, we developed a prototype wireless local area network (WLAN) based system. In our system, a WLAN is deployed outside, and a user wearing a smart phone and monitoring device exercises freely within the coverage area of the wireless network. By doing so, the developed system is able to monitor the activity of each user andmeasures various parameters including those related to exercise adaptation. In a demonstration experiment, the developed system was evaluated and used to monitor users enjoying a Nordic walk, after which users were immediately able to receive their exercise report. In this paper, we discuss the requirements and issues in developing an activity monitoring system and report the findings we obtained through the demonstration experiment.
Designing Picture Book of the Body for 5-6 Year Olds: Teaching Basic Knowledge about Health
Katsura Goto,Michiko Hishinuma,Kazuo Shiraki,Miwako Matsutani,Nobuko Ookubo,Yumi Sakyo,Hisako Nakayama,Akiko Ishimoto,Naoko Arimori,Yoshimi Yamazaki,Kyoko Iwanabe,Takako Shimada,Yasuko Mitsumori,Atsuk 한국간호과학회 2007 한국간호과학회 학술대회 Vol.2007 No.11
Development of a WLAN Based Monitoring System for Group Activity Measurement in Real-Time
Hiroshi Tsunoda,Hidehisa Nakayama,Kohei Ohta,Akihiro Suzuki,Hiroki Nishiyama,Ryoichi Nagatomi,Kazuo Hashimoto,Yuji Waizumi,Glenn Mansfield Keeni,Yoshiaki Nemoto 한국통신학회 2011 Journal of communications and networks Vol.13 No.2
In recent years, there has been a rise in epidemiological evidence suggesting the health benefits of a physically active lifestyle. However, it is not always easy for individuals to personally recognize the optimal conditions for exercise and physical activity. Wearable acceleration-based pedometers have become widely used in estimating the amount of physical activity, and to a limited extent,providing information regarding exercise intensity, but they have never been used to assess adaptation to exercise. In order to realize simultaneous activity monitoring for multiple users exercising outdoors, we developed a prototype wireless local area network (WLAN) based system. In our system, a WLAN is deployed outside, and a user wearing a smart phone and monitoring device exercises freely within the coverage area of the wireless network. By doing so, the developed system is able to monitor the activity of each user andmeasures various parameters including those related to exercise adaptation. In a demonstration experiment, the developed system was evaluated and used to monitor users enjoying a Nordic walk, after which users were immediately able to receive their exercise report. In this paper, we discuss the requirements and issues in developing an activity monitoring system and report the findings we obtained through the demonstration experiment.