http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Kang, Hyun Mi,Kim, Jiyoung,Park, Seah,Kim, Jinyoung,Kim, Haekwon,Kim, Kyung Sik,Lee, Eun Jig,Seo, Sung Ig,Kang, Sung Goo,Lee, Jong-Eun,Lim, Hyunjung Wiley (John WileySons) 2009 Stem Cells Vol.27 No.8
<P>Various attempts have been made to develop stem cell-based therapy to alleviate type I diabetes using animal models. However, it has been a question whether human insulin produced from explanted cells is solely responsible for the normoglycemia of diabetic animals. In this study, we isolated neural crest-like stem cells from the human eyelid fat and examined their therapeutic potentials for diabetes. The human eyelid adipose-derived stem cells (HEACs) displayed characteristics of neural crest cells. Using a two-step culture condition combined with nicotinamide, activin, and/or GLP-1, we differentiated HEACs into insulin-secreting cells and examined in vivo effects of differentiated cells by transplantation experiments. Following differentiation in vitro, HEACs released insulin and c-peptide in a glucose-dependent manner. Upon their transplantation under kidney capsules of streptozotocin-treated immunocompetent mice, we observed normalization of hyperglycemia in 10 of 20 recipient mice until sacrifice after 2 months. Only the human, but not the mouse, insulin and c-peptide were detected in the blood of recipient mice. Removal of the kidneys transplanted with HEACs resulted in a sharp increase of blood glucose level. Removed kidney tissues showed distinct expression of various human genes including insulin, and colocalization of the human insulin and the human nuclear protein in many cells. However, they showed diminished or null expression of some immune-related genes. In conclusion, human insulin alone produced from eyelid-derived stem cells following differentiation into insulin-secreting cells and transplantation could normalize type I diabetes in mice.</P>
A Novel EST with Trench Electrode to Immunize Snab-back Effect and to Obtain High Blocking Voltage
Kang, Ey-Goo,Sung, Man-Young The Korean Institute of Electrical and Electronic 2001 Transactions on Electrical and Electronic Material Vol.2 No.3
A vertical trench electrode type EST has been proposed in this paper. The proposed device considerably improves snapback which leads to a lot of problems of device applications. In this paper, the vertical dual gate Emitter Switched Thyristor (EST) with trench electrode has been proposed for improving snab-back effect. It is observed that the forward blocking voltage of the proposed device is 745V. The conventional EST of the same size were no more than 633V. Because the proposed device was constructed of trench-type electrodes, the electric field moved toward trench-oxide layer, and the punch through breakdown of the proposed EST is occurred at latest.
Subsidence after Anterior Cervical Interbody Fusion Using a Zero-Profile Device
Kang Jae-Seong,Oh Sung-Han,Cho Pyung-Goo 대한말초신경학회 2019 The Nerve Vol.5 No.2
Objective Zero-profile device (Zero-P) has generally been used as a stand-alone cage in anterior cervical discectomy and fusion (ACDF). However, using Zero-P has been reported to be associated with a high subsidence rate. The present study evaluates the factors that influence the subsidence of ACDF. Methods Records of a total of 29 patients with degenerative spondylosis resulting in radiculopathy or myelopathy between C2 and C7 who underwent ACDF with Zero-P were retrospectively reviewed. The participants received 1 or 2 level ACDF with Zero-P in our clinic from December, 2011 to December, 2017. A total of 35 treatment levels were included in the analysis. Subsidence was defined when anterior segmental heights decreased by more than 2mm during the follow-up period. The patients were divided into the subsidence and non-subsidence groups. The following factors were investigated in relation to the occurrence of subsidence: age, gender, body mass index, diabetes mellitus, cage height, perioperative distraction, segmental lordosis, pain score, fusion grade, anterior/posterior segmental height ratio, and cage distance. Results Cage subsidence was observed in 15 cases (15/35, 42%). Cage distance at immediate postoperation was significantly different between the 2 groups; specifically, while the group with subsidence had 2.66±1.62mm distance, the group without subsidence had 1.24±1.13mm distance (p=0.008). Conclusion This study confirms that the more deeply titanium part is inserted at the anterior edge, the more subsidence occurs. In order to decrease the occurrence of cage subsidence, Zero-P cages should be located the closest possible to the anterior rim of vertebral body at operation.
Utilization of the Bombyx mori Hypothetical Protein 32 Promoter for Efficient Transgene Expression
Goo, Tae-Won,Kim, Sung-Wan,Kim, Seong-Ryul,Park, Seung-Won,Kang, Seok-Woo,Lee, Kwang-Gill,Kwon, O-Yu,Yun, Eun-Young Korean Society of Sericultural Science 2010 International Journal of Industrial Entomology Vol.20 No.2
For stable germline transformation, the promoter of Bombyx mori cytoplasmic actin gene (BmA3) has been used for ubiquitous expression of transgenes. So far, no strong promoter is available for ubiquitous expression in B. mori, excluding BmA3 promoter. To identify more powerful promoter than previously reported BmA3 promoter, we isolated 9 clones that show stronger signal compared to BmA3 by a dot blot hybridization. Among these 9 clones, we focused on one clone which has high amino acid homology (85%) with hypothetical protein 32 gene of Lonomia obliqua. This clone, named bHp32 (B. mori hypothetical protein 32) was ubiquitously expressed in all tissues and developmental stage of fifth instar B. mori larvae. As result of promoter assay using dual luciferase assay system, we found the highest transcription activity region (-1,200/+220) in the 5'-flanking region of bHp32 gene, which has 42-fold more intensive promoter activity than BmA3 promoter. Moreover, the bHp32 promoter was normally regulated in Bm5, Sf9, and S2 cells. Therefore, we suggest that bHp32 promoter may be used more powerful and effectively for transgene expression in various insects containing B. mori as a universal promoter.
Performance Evaluation of Sprayed Ductile Fiber-Reinforced Mortar as a Repairing Material
Kang, Su-Tae,Koh, Kyung-Taek,Ryu, Gum-Sung,Kim, Jin-Soo,Han, Cheon-Goo Korea Concrete Institute 2008 International Journal of Concrete Structures and M Vol.2 No.1
Most of existing repair materials have some shortcomings such as brittle fracture, imperfect interface bonding and marked difference in modulus of elasticity compared with the structures. These problems make their repair inefficient. Some researches on using a fiber-reinforced mortar as an alternative to enhance the efficiency have been carried out recently. This paper presents the results of an experimental study on the performance of sprayed PVA fiber-reinforced mortar as a repair material. We evaluated its mechanical properties, durability and strengthening effect. This study shows that the sprayed PVA fiber-reinforced mortar is remarkably effective as a repair material.