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Identification and expression analysis of SQUAMOSA promoter-binding protein (SBP) genes in mungbean
Zhang Huiying,Guo Xu,Wang Zheng,Yu Zhaohang,Nie Kaiyue,Han Meng,Xu Xuexin,Zhu Hong 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.3
SQUAMOSA promoter-binding protein (SBP) genes are important transcription factors involved in plant growth and development. However, the information about mungbean VrSBP genes is limited. In this study, we identified and characterized 22 VrSBP genes in the mungbean genome. Many characteristics of VrSBP genes were analyzed, and most VrSBP genes were considered to be expressed only in the nucleus. VrSBP genes were grouped into 9 subfamilies, with the exception of the g7 and ms subfamilies which are distinctive to Poaceae species. The g3 subfamily was the largest one with 6 VrSBP members. Eight segmental events were found among VrSBP genes, and all VrSBP duplicated genes were evolved via purification selection. The gene structure of VrSBP genes in the same subfamily was similar, and the conserved motifs are different among different subfamilies of VrSBP genes. Additionally, 12 VrSBP genes were considered to be the target genes of vra-miR156a. The types and numbers of cis-acting elements in VrSBP promoters were varied and VrSBP genes showed different expression patterns in different tissues. In addition, the protein interaction network revealed that VrSBP genes were involved in many development processes. Our study has provided important information which will allow the further functional characterization of VrSBP genes.
Mengqi Wang,Kaiyue Zhang,Feixiang Ji,Yurong Guo,Chao Wang,Shiping Wang,Ya Chu,Guangjiu Zhao 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.104 No.-
A water-soluble probe composite material (CDs/ZnO) was synthesized by microwave pyrolysis methodsof citric acid, urea and zinc oxide (ZnO) with aggregation-induced emission activity. We creatively realizedthe composite of CDs and nano-ZnO in the form of foam, then used as bactericide for the first timeand showed excellent antibacterial properties. Also, the composite materials overcame the problem ofaggregation-induced quenching (ACQ) and realized solid-state emission. The materials had two differentemission centers, and the two emission peaks were varied with the change of excitation wavelength. Furthermore, Methyl red (MR) showed fluorescence quenching for CDs/ZnO with obvious linear correlation,which demonstrated potential in the measurement of MR in dyeing wastewater. The CDs/ZnO weobtained perfectly inherited the advantages of CDs and nano ZnO, it has not just no cytotoxicity to cells,but also has a good killing effect on bacteria. Hence, this work shows significant adaptable fluorescencebasedprotocol with enormous potential applications in biology.
Nana Shi,Kaiyue Sun,Zedi Zhang,Juan Zhao,Lina Geng,Yuhua Lei 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.101 No.-
Carbon dot (CDs) were prepared for carrying DOX (Doxorubicin) or FAC (Ferric ammonium citrate), andthe loading mechanism and release kinetics of CDs-drugs were studied. CDs were prepared using peonypollen and urea, and characterized by TEM, XRD, 13C NMR, XPS, UV, FT-IR, DLS and zeta potential. Theobtained CDs were rich of –NH2 group on the surface, amorphous phase, spherical morphology withthe diameter about 5 nm, and had a blue fluorescence with the quantum yield (QY) of 12%. Iron disorderscan cause cancer or anemia. DOX is used for treating cancer, but has severe side effects. FAC is an ironsupplement agent, but had low bioavailability. CDs carried DOX (or FAC) through –N=C– (or –CONH–)bond, had high loading efficiency for DOX (37.2%) and FAC (54.0%) by optimizing mass ratios of mCDsto mdrug and reacted overnight. Cellular experiments showed that CDs-drugs had lower cytotoxicity thanfree drugs and CDs co-located with the drug. In vitro release indicated that CDs-DOX had pH-targetedproperty and CDs-FAC could be absorbed easily. CDs-drug release belonged to Weibull model, and releasemechanism was determined by the bonding way between CDs and drug. CDs had potential applicationsin delivery drugs with –C=O or –COOH group.