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- 저자 : Kaili Sun (검색결과 4 건)
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Cloning and Sequence Analysis of Wild Argali ISG15 cDNA
Sun, Yanming,Chen, Kaili,Shen, Wen,Cui, Rupeng,Lu, Haifu Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.4
The complete coding sequence of Wild Argali ISG15 cDNA was generated by rapid amplification of cDNA ends. The ISG15 cDNA was 642 bp with an open reading frame of 474 bp, which encoded a 17.47 kDa protein composed of 157 amino acids. Its amino acid sequence shared 97.9%, 80.8%, 91.4%, 94.3%, 78.3% identity with those of ISG15cDNA from Ovis aries (accession no. NM001009735.1), Capra hircus (accession no. HQ329186.1), Bos taurus (accession no. BC102318.1), Bubalus bubalis (accession no. HM543269.1), and Sus scrofa (accession no. EU647216.1), respectively. The entire coding sequence was inserted into the pET-28a vector and expressed in E. coli. The recombinant protein corresponded to the expected molecular mass of 25 kDa as judged by SDS-PAGE, and it was detected in the bacterial inclusion bodies. The expressed protein could be purified by $Ni^{2+}$ chelate affinity chromatography and the results from the lymphocyte proliferation test showed that the product could stimulate lymphocyte proliferation very well (p<0.05), which further confirmed its biological activity.
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Cloning and sequence analysis of Wild Argali short palate, lung and nasal epithelium clone 1 cDNA
Wen Shen,Kaili Chen,Yanming Sun,Haiying Guo,Dongmei Chen,Yang Cao 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.5
Objective: Experiments were conducted to clone the sequence of Wild Argali short palate, lung and nasal epithelium clone 1 (SPLUNC1) cDNA, and to lay the foundation for further study the biological function of Wild Argali SPLUNC1. Methods: The complete sequence of Wild Argali SPLUNC1 cDNA was generated by rapid amplification of cDNA ends. The entire coding sequence was inserted into the pPIC9K vector and expressed in Pichia pastoris (P. pastoris) GS115. The recombinant SPLUNC1 protein was detected by Western blot and purified by Ni2+ chelate affinity chromatography. The test of effect of the protein on Mycoplasma ovipneumoniae (MO) was performed with real-time polymerase chain reaction. Results: The Wild Argali SPLUNC1 cDNA was 1,076 bp with an open reading frame of 768 bp, which encoded a 26.49 kDa protein composed of 255 amino acids. Its amino acid sequence shared 98.4%, 96.9%, 94.5%, 90.2%, 80.8%, 78.4%, 78.3%, 72.5%, 72.3%, 68.8% identity with those of SPLUNC1 cDNA from Ovis aries (accession no. NP_001288334.1), Capra hircus (accession no. XP_005688516.1), Pantholops hodgsonii (accession no. XP_005979709.1), Bos taurus (accession no. NP_776851.1), Felis catus (accession no. XP_006929910.1), Homo sapiens (accession no. NP_001230122.1), Sus scrofa (accession no. NP_001005727.1), Chinchilla lanigera (accession no. NP_001269294.1), Mus musculus (accession no. NP_035256.2), and Rattus norvegicus (accession no. NP_742028.1), respectively. The recombinant protein corresponded to the expected molecular mass of 25.47 kDa as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and it was detected in the supernatant of P. pastoris, and it could be purified. The results from the test of inhibition effect of argali recombinant SPLUNC1 protein on MO showed that the product could inhibit MO very well (p<0.01). Conclusion: The amino acid sequence of Wild Argali SPLUNC1 was different from other organisms. The recombinant SPLUNC1 protein has good biological activity.
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Kaihang Wang,Kaili Sun,Zihan Li,Zunhang Lv,Tianpeng Yu,Xin Liu,Guixue Wang,Guangwen Xie,Luhua Jiang 대한금속·재료학회 2020 ELECTRONIC MATERIALS LETTERS Vol.16 No.2
The development of electrocatalysts with high activity and low Tafel slope for overall water splitting has become a crucialchallenge to exploit the sustainable energy. Herein, we construct a Fe–Co–P–Gr catalyst on nickel foam (NF) support throughelectroless composite plating to realize the co-deposition of Fe–Co–P alloys and graphene quantum dots. Interestingly,graphene quantum dots exhibit obvious eff ects on electron mobility and active sites of Fe–Co–P–Gr/NF catalyst. In oxygenevolution reaction, the Fe–Co–P–Gr/NF catalyst exhibits a small overpotential of 230 mV at 10 mA cm −2 and fast kineticswith Tafel slope of 37.8 mV dec −1 . Meanwhile, the Fe–Co–P–Gr/NF also has a superior hydrogen evolution reaction performancein 1.0 M KOH. Compared with the Fe–Co–P alloys, the Fe–Co–P–Gr/NF both as the anode and cathode requireonly 1.58 V to reach a current density of 10 mA cm −2 . The successful preparation of Fe–Co–P–Gr/NF electrode throughelectroless composite deposition provides a new path to manufacture electrocatalysts for overall water splitting.
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Performance Analysis of Three-Phase Phase-Locked Loops for Distorted and Unbalanced Grids
Kai Li,An Bo,Hong Zheng,Ningbo Sun 전력전자학회 2017 JOURNAL OF POWER ELECTRONICS Vol.17 No.1
This paper studies the performances of five typical Phase-locked Loops (PLLs) for distorted and unbalanced grid, which are the Decoupled Double Synchronous Reference Frame PLL (DDSRF-PLL), Double Second-Order Generalized Integrator PLL (DSOGI-PLL), Double Second-Order Generalized Integrator Frequency-Lock Loop (DSOGI-FLL), Double Inverse Park Transformation PLL (DIPT-PLL) and Complex Coefficient Filter based PLL (CCF-PLL). Firstly, the principles of each method are meticulously analyzed and their unified small-signal models are proposed to reveal their interior relations and design control parameters. Then the performances are compared by simulations and experiments to investigate their dynamic and steady-state performances under the conditions of a grid voltage with a negative sequence component, a voltage drop and a frequency step. Finally, the merits and drawbacks of each PLL are given. The compared results provide a guide for the application of current control, low voltage ride through (LVRT), and unintentional islanding detection.
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