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RISS 인기검색어
- 검색키워드
- 저자 : Kagayaki Morishima (검색결과 3 건)
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Genetic structure of wild brown sole inferred from mitochondrial DNA analysis
김상규,Kagayaki Morishima,Katsutoshi Arai 한국통합생물학회 2010 Animal cells and systems Vol.14 No.3
The population structure of brown sole was examined in a total of 308 samples collected from three geographical groups: one locality (Gangneung) on the east side of the Korean Peninsula, two localities (Erimo and Tomakomai)on the southeastern side and four localities (Onishika, Teshio, Tomamae and Yoichi) on the northwestern side of Hokkaido Island, Japan, by using sequences of 484 bp from the 5' end of the control region of mtDNA. We detected 225 haplotypes, but 183 of them were unique to an individual. A total of 116 nucleotide sites were variable. Haplotype diversity (h) was very high, ranging from 0.989 to 1.000, and nucleotide diversity (π) was detected between 0.015 and 0.022. Genetic distances (ΦST) within populations, among populations and among geographical groups were low (0.0002 to 0.0014). No significant difference was detected by the AMOVA test (PB0.05). Pairwise FST values between sampling localities were also low and not significant. Genetic differentiation was not detected among sampling localities.
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Genetic structure of wild brown sole inferred from mitochondrial DNA analysis
Kim, Sang-Gyu,Morishima, Kagayaki,Arai, Katsutoshi The Korean Society for Integrative Biology 2010 Animal cells and systems Vol.14 No.3
The population structure of brown sole was examined in a total of 308 samples collected from three geographical groups: one locality (Gangneung) on the east side of the Korean Peninsula, two localities (Erimo and Tomakomai) on the southeastern side and four localities (Onishika, Teshio, Tomamae and Yoichi) on the northwestern side of Hokkaido Island, Japan, by using sequences of 484 bp from the 5' end of the control region of mtDNA. We detected 225 haplotypes, but 183 of them were unique to an individual. A total of 116 nucleotide sites were variable. Haplotype diversity (h) was very high, ranging from 0.989 to 1.000, and nucleotide diversity (${\pi}$) was detected between 0.015 and 0.022. Genetic distances (${\Phi}_{ST}$) within populations, among populations and among geographical groups were low (0.0002 to 0.0014). No significant difference was detected by the AMOVA test (P < 0.05). Pairwise $F_{ST}$ values between sampling localities were also low and not significant. Genetic differentiation was not detected among sampling localities.
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JEONG, Dal-Sang,GONZALEZ, Enrique Blanco,MORISHIMA, Kagayaki,ARAI, Katsutoshi,UMINO, Tetsuya Springer Japan KK 2007 Fisheries Science Vol.73 No.4
<P>ABSTRACT: </P><P>The genetic contribution of 51 broodstock, comprising 29 females and 22 males, reared at Hiroshima City Marine Products Promotion Center for the production of stocked black sea bream was monitored during two consecutive years using seven microsatellite DNA loci. The high discrimination ability of these markers was reflected in the polymorphic identification content (<I>PIC</I> = 0.831), the exclusion probability (<I>Q</I> ∼ 1), and the low probability of identity index (<I>I</I> = 3.635<SUP>−10</SUP>). The total number of breeders contributing to the mating process was estimated at 32 (62.7%) in 2000 and 30 (58.8%) in 2001. On pedigree reconstruction, 69.3% of the offspring were successfully assigned to a single broodstock pair. Loss of alleles accounted for 16.9% during seed production; nevertheless, 90.9% of males and 69.0% of females participated in the mating process. Based on microsatellite genetic tagging, 58.9% of the fish sampled during the two months after release were identified as hatchery stock, presenting no significant differences from wild conspecifics in either fork length or body weight.</P>
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