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- 저자 : Jun Asanuma (검색결과 4 건)
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Structural evidence of <b>α</b>‐aminoacylated lipoproteins of <i>Staphylococcus aureus</i>
Asanuma, Miwako,Kurokawa, Kenji,Ichikawa, Rie,Ryu, Kyoung‐,Hwa,Chae, Jun‐,Ho,Dohmae, Naoshi,Lee, Bok Luel,Nakayama, Hiroshi Blackwell Publishing Ltd 2011 FEBS JOURNAL Vol.278 No.5
<P>Bacterial lipoproteins are known to be diacylated or triacylated and activate mammalian immune cells via Toll‐like receptor 2/6 or 2/1 heterodimer. Because the genomes of low G+C content Gram‐positive bacteria, such as <I>Staphylococcus aureus</I>, do not contain <I>Escherichia coli</I>‐type apolipoprotein <I>N</I>‐acyltransferase, an enzyme converting diacylated lipoproteins into triacylated forms, it has been widely believed that native lipoproteins of <I>S. aureus</I> are diacylated. However, we recently demonstrated that one lipoprotein SitC purified from <I>S. aureus</I> RN4220 strain was triacylated. Almost simultaneously, another group reported that another lipoprotein SA2202 purified from <I>S. aureus</I> SA113 strain was diacylated. The determination of exact lipidated structures of <I>S. aureus</I> lipoproteins is thus crucial for elucidating the molecular basis of host–microorganism interactions. Toward this purpose, we intensively used MS‐based analyses. Here, we demonstrate that SitC lipoprotein of <I>S. aureus</I> RN4220 strain has two lipoprotein lipase‐labile <I>O</I>‐esterified fatty acids and one lipoprotein lipase‐resistant fatty acid. Further MS/MS analysis of the lipoprotein lipase digest revealed that the lipoprotein lipase‐resistant fatty acid was acylated to α‐amino group of the N‐terminal cysteine residue of SitC. Triacylated forms of SitC with various length fatty acids were also confirmed in cell lysate of the RN4220 and Triton X‐114 phase in three other <I>S. aureus</I> strains, including SA113 strain and one <I>Staphylococcus epidermidis</I> strain. Moreover, four other major lipoproteins including SA2202 in <I>S. aureus</I> strains were identified as <I>N</I>‐acylated. These results strongly suggest that lipoproteins of <I>S. aureus</I> are mainly in the <I>N</I>‐acylated triacyl form.</P>
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Suzuki, Rikie,Hiyama, Tetsuya,Asanuma, Jun,Ohata, Tetsuo,Koike, Toshio 대한원격탐사학회 2001 International Symposium on Remote Sensing Vol.17 No.1
On 9 days from April to June, 2000, airborne observations of the land surface were carried out around Yakutsk in eastern Siberia. A video camera and spectrometer were installed in the aircraft, and the non-homogeneous land surface was observed frown 100 and 150m flight heights. From the video image, the land surface was classified by a visual interpretation, and derived the proportional area of land cover types in the area. It was revealed that the left bank terrace of Lena River has more birch forest area and less no-forest area than the right bank terrace. From the spectral reflectance measurement, it was revealed that the spectral characteristics in near-infrared and visible bands has a great potential to analyze the surface vegetation property by combining the video camera data.
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동아시아 지역의 플럭스 타워 관측지에 대한 MODIS 위성영상 기반의 증발산 평가
Jeong, Seung-Taek,Jang, Keun-Chang,Kang, Sin-Kyu,Kim, Joon,Kondo, Hiroaki,Gamo, Minoru,Asanuma, Jun,Saigusa, Nobuko,Wang, Shaoqiang,Han, Shijie 한국농림기상학회 2009 한국농림기상학회지 Vol.11 No.4
지표 증발산은 육상 생태계의 수문순환의 주요 성분으로서, 지표-대기간의 에너지 교환, 미기후, 지역의 수자원 함량, 식생의 일차생산성 등에 중요한 영향을 미친다. 증발산을 추정하기 위한 방법들 중에서 MODIS를 이용한 방법은 위성 자료만을 사용하여 넓은 지역에 대한 지속적인 증발산 모니터링이 가능하다는 장점을 갖고 있다. 본 연구에서는 MODIS 기반의 증발산 추정 알고리즘을 동아시아 지역에 적용하고, 그 신뢰도를 평가하였으며, 주요 오차요인을 분석하였다. 증발산 평가 결과 여섯 연구지역(GDK, HFK, TKY, TMK, CBS, SKT)에서는 $r^2$가 0.38~0.73, ME 와 RMSE가 각각 $-44{\sim}+31W\;m^{-2}$, $48{\sim}111W\;m^{-2}$로 신뢰할 만한 결과를 나타냈다. 하지만 다른 세 연구지역(HBG, QYZ, MKL)에서는 관측 값과 비교해서 차이를 나타내었고, 과소평가하는 경향을 보였다. HBG, MKL 지역은 MODIS 기상 자료 및 복사요소의 오차가 주요 원인으로 나타났다. 그러나 QYZ지역은 기상 자료와 복사요소가 모두 좋은 일치도를 보였기 때문에, 모형의 모수와 관련된 오차가 주요 원인의 하나로 판단된다. 임관 전도도의 오차가 증발산 오차에 미치는 영향을 분석한 결과, HBG지역을 제외한 다른 연구지 역에서 r값이 0.59~0.82로 관측값과의 상관성이 높은 것을 확인하였다. 또한 MODIS로부터 산출된 순간 증발산을 일 단위로 확장시킨 결과, 순간 증발산의 일치도가 떨어졌던 3개 연구지역을 제외하고 6개 지역에서 $r^2$가 0.44~0.89, ME와 RMSE는 각각 $-0.7{\sim}+0.6mm\;day^{-1}$, $0.5{\sim}1.1mm\;day^{-1}$의 범위로 신뢰도 있는 결과를 나타냈다. Evapotranspiration (ET) is one of the major hydrologic processes in terrestrial ecosystems. A reliable estimation of spatially representavtive ET is necessary for deriving regional water budget, primary productivity of vegetation, and feedbacks of land surface to regional climate. Moderate resolution imaging spectroradiometer (MODIS) provides an opportunity to monitor ET for wide area at daily time scale. In this study, we applied a MODIS-based ET algorithm and tested its reliability for nine flux tower sites in East Asia. This is a stand-alone MODIS algorithm based on the Penman-Monteith equation and uses input data derived from MODIS. Instantaneous ET was estimated and scaled up to daily ET. For six flux sites, the MODIS-derived instantaneous ET showed a good agreement with the measured data ($r^2=0.38$ to 0.73, ME = -44 to $+31W\;m^{-2}$, RMSE =48 to $111W\;m^{-2}$). However, for the other three sites, a poor agreement was observed. The predictability of MODIS ET was improved when the up-scaled daily ET was used ($r^2\;=\;0.48$ to 0.89, ME = -0.7 to $-0.6\;mm\;day^{-1}$, $RMSE=\;0.5{\sim}1.1\;mm\;day^{-1}$). Errors in the canopy conductance were identified as a primary factor of uncertainty in MODIS-derived ET and hence, a more reliable estimation of canopy conductance is necessary to increase the accuracy of MODIS ET.
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