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RISS 인기검색어
- 검색키워드
- 저자 : Juan-Fang Han (검색결과 6 건)
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Quantitative Indirect ELISA for Determination of Walnut Proteins in Foods
Juan Fang,Dan Chen,Chaoyin Chen,Feng Ge,Diqiu Liu,Benyong Han,Xiangfeng Xiong 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.6
An indirect enzyme-linked immunosorbent assay (ELISA) capable of detecting walnut proteins in commercial products was developed. Polyclonal antibodies against soluble walnut protein were used. Specific epitopes of walnut protein were analyzed for cross activity with peanut, soybean, sesame, rice, wheat, jujube, and defatted milk protein. Epitopes of walnut proteins had no cross activity. The indirect ELISA was highly specific for soluble walnut proteins. Recovery values from walnut protein solutions ranged from 88.47 to 115%, and low coefficients of variation (<10%) indicated good repeatability. Intra and inter-assay precisions were <6 and <7 %, respectively. The limit of detection was 10 ng/mL of soluble walnut protein. The method was applied to detect some commercial food products in China and the results showed that indirect ELISA could be promisingly used to quantify adulteration of walnut processed foods.
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Preparation and Thermal Performance of Fullerene-Based Lead Salt
Guan, Hui-Juan,Peng, Ru-Fang,Jin, Bo,Liang, Hua,Zhao, Feng-Qi,Bu, Xing-Bing,Han, Wen-Jing,Chu, Shi-Jin Korean Chemical Society 2014 Bulletin of the Korean Chemical Society Vol.35 No.8
$C_{60}$ is widely investigated because of its unique structure. But its applications in solid propellant seem to be relatively neglected. $C_{60}$ has more outstanding features than carbon black which is widely used as a catalyst ingredient of solid propellant. To combine the advantages of fullerene and lead salts, another good composite in propellant catalysts, we synthesized a kind of fullerene phenylalanine lead salt (FPL) and explored its thermal performances by differential thermal analysis (DTA) and thermogravimetry analysis (TGA). The results show it undergoes four exothermic processes started from 408 K. Combined TGA and X-ray diffractometer (XRD), the decomposition mechanism of FPL was derived by TG-IR and comparing IR spectra of FPL and its residues after burned to $327^{\circ}C$, $376^{\circ}C$ and $424^{\circ}C$, respectively. Effect of FPL on the decomposition characteristic of hexogen (RDX), a type of explosive in propellant, has been investigated using DTA at different heating rate, which shows the decomposition temperatures of the explosive are all reduced by more than 20 K. The corresponding activation energy ($E_a$) is decreased by $30kJ{\cdot}mol^{-1}$. So FPL has potential application as a combustion catalyst in solid propellant.
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Yang, Xiao-Ping,Han, Yue-Dong,Ye, Jian-Jun,Chen, Gang,Luo, Ying,Ma, Hong-Xia,Yu, Xue-Wen,Niu, Juan-Qin,Ren, Fang-Yuan,Guo, You-Ming Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.12
Background: As a common and essential contrast medium at present, gadobenate dimeglumine has shown better performance than some other agents when applied to Breast Magnetic Resonance Imaging Screening (Breast MRI Screening). Nevertheless, reports on the diagnostic performance of these two mediums (gadobenate dimeglumine and gadopentetate dimeglumine) are not completely consistent. Objective: To assess the diagnostic value of gadobenate dimeglumine and gadopentetate dimeglumine for Breast MRI Screening in patients suffering from breast cancer and to provide more convinced evidence to guide clinical practice in terms of appropriate contrast agents. Data Sources and Review Methods: Original articles in English and Chinese published before January 2013 were selected from available databases (The Cochrane Library, PUBMED, EMBASE, Chinese Biomedical Literature Database, Chinese Scientific Journals Full-text Database, Chinese Journal Full-text). The criteria for inclusion and exclusion were based on the standard for diagnosis tests. Meta-Disc software (Version 1.4) was used for data analysis. Then, the area under curve (AUC) of SROC and the spearman rank correlation of sensitivity against (1-specificity) were calculated. Results: Total of 17 researches involving 1934 patients were included. The pooled sensitivity of gadobenate dimeglumine and gadopentetate dimeglumine were 0.99 (0.97, 1.00) and 0.93 (0.88, 1.00) respectively. The pooled specificity for these two contrast agents were 0.924 (0.902, 0.943) and 0.838 (0.817, 0.858) respectively, and the AUC of SROC curve were 0.9781 and 0.9215 respectively. Conclusions: Gadobenate dimeglumine can be regarded as a more effective and feasible contrast medium for Breast MRI Screening. At least 5% differences in diagnostic performance are usually considered as clinically relevant.
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Head-on Collision Between Two Envelope Solitary Waves in a Granular Medium
Wen-Qing Du,Jian-An Sun,Juan-Fang Han,Wen-Shan Duan,Yang-Yang Yang,Xin Jiang 한국물리학회 2019 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.75 No.11
We investigated the head-on collision between two envelope solitary waves. Head-on collisions between two envelope solitary waves are first discussed in one-dimension (1D) granular chains. The interesting result is that no phase shift or phase delay detected after the head-on collision between two envelope solitary waves. The maximum amplitude during the head-on collision between two envelope solitary waves is also found to be less than the sum of the amplitudes of the two envelope solitary waves, but is larger than the amplitude of the either of the envelope solitary waves.
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AZD1480 Can Inhibit the Biological Behavior of Ovarian Cancer SKOV3 Cells in vitro
Sun, Zhao-Ling,Tang, Ya-Juan,Wu, Wei-Guang,Xing, Jun,He, Yan-Fang,Xin, De-Mei,Yu, Yan-Li,Yang, Yang,Han, Ping Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.8
Objective: To study the mechanism of effects of AZD1480 on the SKOV3 ovarian cancer cell line. Methods: The MTT method was used to assess cellular proliferation, flow cytometry for cellular apoptosis, the scratch test to determine migration, transwell chamber assays to detect cellular invasion, plate clone experiments to detect the clone forming ability and Western blotting to determine p-STAT3 protein levels. Results: The proliferation rate, migration ability, invasiveness and the clone forming ability of SKOV3 cells were reduced after treatment with AZD1480, while apoptosis rate and chemotherapeutic susceptibility were increased. After treatment with AZD1480 plus cisplatin, the apoptosis rate increased significantly while the expression level of p-STAT3 protein was decreased. Conclusion: AZD1480 can inhibit the proliferation, invasion, metastasis and clone formation of SKOV3 cells, induce cellulsar apoptosis, increase the chemotherapeutic sensitivity and reduce the expression level of p-STAT3 protein.
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Podcasting and Politics in Singapore: An Experimental Study of Medium Effects
Skoric, Marko M.,Sim, Clarice,Juan, Han Teck,Fang, Pam World Association for Triple Helix and Future Stra 2009 Journal of Contemporary Eastern Asia Vol.8 No.2
A ban on political podcasting during the General Elections 2006 in Singapore was justified by the Singaporean government on the grounds that the new medium had a greater power to influence voters than traditional modes of political discourse. A between-subjects controlled experiment was conducted to test whether podcasts of political speeches had a greater power to influence voters' evaluations of political candidates and likelihood of voting for them than online text-based transcripts of the same speeches. The study also examined whether mere exposure to political speeches online, irrespective of the modality, had an effect on voters' more general political preferences, i.e. the likelihood of support and voting for the opposition. The findings suggest that political podcasts were no more persuasive than text-based websites and that the effects on political preferences, if any, were likely due the exposure to political content online, not because of the nature of the medium. The implications of the findings are discussed.
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