Identification of a Novel SHP-2 Protein Tyrosine Phosphatase Inhibitor

Ju, A.,Seo, H.,Kim, H.,Park, B.C.,Park, S.G.,Kim, J.H.,Choi, H.-K.,Min, K.H.,Cho, S. THE JAPAN PUBLICATIONS TRADING CO LTD 2014 Bulletin of the Chemical Society of Japan Vol.87 No.3

The Src homology 2 (SH2) domain-containing phosphatase 2 (SHP-2) is a nonreceptor protein tyrosine phosphatase (PTP) involved in extracellular-regulated kinase (ERK) activation. Recent studies have shown that gain-of-function mutations in SHP-2 are associated with several diseases, including LEOPARD syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. In this study, we identified the novel SHP-2 inhibitor 3-(1-benzimidazolylmethyl)-6-p-tolyl-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazole (MLS-001). SHIP-2 activity was inhibited by MLS-001, whereas other types of PTPs, namely ACP1, CDC25A, DUSP3, DUSP14, DUSP18, DUSP22, DUSP23, DUSP26, and SSH3, were not. Furthermore, TCPTP and SHP-1 that are closely related to SHP-2 were not inhibited by the inhibitor. Kinetic studies with MLS-001 and SHP-2 revealed a competitive inhibition. The SHP-2 expressing cells treated with MLS-001 demonstrated reduced SHP-2 phosphatase activity, thereby suggesting that MLS-001 effectively passes through cell membranes. In addition, MLS-001 reduced SHP-2-mediated phosphorylation in the activation loop of ERK in cells. Therefore, MLS-001 could be a lead compound for developing a potent SHP-2 inhibitor.

Anticancer effects of methanol extract of Myrmecodia platytyrea Becc. leaves against human hepatocellular carcinoma cells via inhibition of ERK and STAT3 signaling pathways

Ju, A.,Cho, Y.-C.,Kim, B. R.,Lee, S.,Le, H. T. T.,Vuong, H. L.,Cho, S. Spandidos Publications 2018 International journal of oncology Vol.52 No.1

Developmental profile of human frontal cortex using glycomic approach

Jua Lee,Jae Yun Hwang,Sum in Kim,Hyun Joo An 한국당과학회 2017 한국당과학회 학술대회 Vol.2017 No.01

Glycan plays numerous roles in the nervous system during development, regeneration and synaptic plasticity, forming of a complex meshwork at the cell surface. We newly established an analytical platform for exploring two molecules- glycan attached to proteins and glycan attached to lipids- from human brain using mass spectrometry. 69 brain samples were obtained from the University of Cambridge. Glycan extraction method from previous study, and new ganglioside extraction method were paired with MS. Briefly, each brain tissues were grinded by sonication. Then for glycans, the membrane was pelleted by ultracentrifugation. N-glycan release and associated processing steps were performed by previously optimized procedures. For sphingolipids, homogenized brain is mixed with chloroform/methanol/water and centrifuged. Total lipids extracts were partitioned again with chloroform/methanol to collect gangliosides. After further enrichment by SPE, N-glycans and gangliosides were profiled by positive and negative ion mode nano-LC/MS respectively. Stucture information was obtained by nano-LC/MS/MS. Brain samples were divided into the 7 groups: neonates, infants, toddlers, school-ages, teenagers, young adults. We successfully profiled brain glycome and sphingolipids, identifying approximately 100 N-glycan compositions and 120 ganglioside compounds with several unique “brain type” characters. This study promise insights into involvement of glycans to massive flow of information that underpins brain, with providing meaningful understanding to neuropathology related with aging.

Designation of fingerprint glycopeptides for targeted glycoproteomic analysis of serum haptoglobin : insights into gastric cancer biomarker discovery

Jua Lee,Serenus Hua,Sung Hyeon Lee,Myung Jin Oh,JaekyungYun,Jin Young Kim,Jae-Han Kim,Jung Hoe Kim,Hyun Joo An 한국당과학회 2018 한국당과학회 학술대회 Vol.2018 No.01

Gastric cancer (GC) is one of the leading causes of cancer-related death worldwide, largely because of difficulties in early diagnosis. In spite of accumulating evidence linking aberrant glycosylation and GC, site-specific localization of the glycosylation for improving clinical specificity and sensitivity is still in analytical challenge. Here, we constructed an analytical platform with targeted glycoproteomic approach for GC biomarker discovery. Unlike conventional glycomic approach characterized by untargeted MS profiling of released glycan, developed platform can be characterized by three key features: target protein-specific, glycosylation site-specific, and structure-specific platform with one-shot non-specific enzyme digestion. Serum haptoglobin (Hp) was enriched by immunoaffinity purification and subjected to pronase to generate site-specific glycopeptides. Glycopeptides were identified and quantified by nano LC/MS and -LC/MS/MS, resulting in full characterization of glycan macro- and micro-heterogeneity. A total of 96 glycopeptides, each corresponding to a unique glycan/glycosite pairing, were tracked across all cancer and control samples. Significant alterations in site-specific glycosylation have been observed in Hp in GC patients.

신경증의 기록과 염인증자(厭人症者)의 연서쓰기 -김유정 문학에 나타난 죽음충동과 에로스

정주아 ( Jua Joung ) 한국문학연구학회 2015 현대문학의 연구 Vol.0 No.57

이 논문은 김유정의 소설이 위기 상황에 놓인 인간의 심리를 묘사하는 데 탁월하다는 지점에서 출발한다.김유정은 피폐해진 농촌이나 들병이로 나선 여성들처럼 식민치하 농촌의 현실을 고발하는 계몽적 주제를 선택하지만, 정작 그가 써낸 서사는 주어진 삶의 방식을 스스로 파괴해버리는 일탈의 순간과 사회적 질서 밖의 자유를 옹호하고 동경하는 감성적 충동으로 마무리되는 경향이 있다.이때 이러한 일탈을 감행하지 못하는 일상적인 인물군상은 현실의 폭력을 감내하는 과정에서 심리적으로 병든 신경증자들이 된다.일반적으로 김유정의 소설은 토속성과 해학성 등의 범주에서 읽혀 왔다.그러나 이 논문에서는 농촌이 ‘땅과 가족’이라는 전통적인 가치를 절대시하고 그에 따른 역할론이 확고한 탓에 변화에 매우 보수적인 공간이라는 점, 이에 외적 조건의 변화에도 불구하고 동일한 삶의 양상을 유지하려는 관성이 강해서 그만큼 심리적 억압이 큰 공간이 된다는 점에 주목하였다.이글은 김유정 소설에 등장하는 인물군상이 지닌 파괴와 해체의 충동이 곧작가 자신도 ‘염인증’이란 이름으로 불렀던 죽음충동과 연결된다고 보고, 김유정의 문학과 삶을 광적인 ‘연애편지 쓰기’, 즉 생의 충동으로서의 에로스로 죽음충동을 극복하려 시도하는 대립구도 속에서 분석해보았다. Kim You Jeong is known as a novelist who focused upon rural life under Japanese colonial rule. He had written many short stories about poor farmers who had lost their farm land, and had written about farmer’s wives who had to prostitute themselves in order to provide for their families. This essay approaches his novels from a psychoanalytic perspective, and not from a point of view realistic. His life was a series of misfortunes. He had suffered the death of his mother, the financial ruin and bankruptcy of his family’s fortune, crushing poverty, and chronic disease. Kim used to say that ‘writing novels was a way to overcome his fate.’ This essay tries to analyze his novels in a confrontation between the death drive and the life drive(eros). Naturally, we can hear the author’s voices in his criticism of colonial exploitation policy and his concern over rural poverty in his novels. At the same time, we can also find characters who were tempted by the deviation and release from previously accepted norms. For example, the farmers had felt frustrated due to the loss of their farm land, meanwhile, they felt like a burden had been lifted from their shoulders. They went back to a much more simpler life without the desire to acquire material things or having to resort to tenant farming. While farmers and their families tried to fit themselves into the social order, more often than not, they suffered from mental disorders due to the misery of poverty. Many of Kim’s literary works focus on life in rural spaces. Many scholars have lauded Kim’s works as being apt in their bucolic and idyllic depictions of rural life. Some others have lauded his works as being apt in their realistic and detailed depictions of rural life in colonial period. But this essay takes note that the members of rural communities were under the stressful social pressure. They are so conservative in change of social norms as much as they think normative customs related to family and farmland are absolutely important. In his novels, Kim is able to brilliantly portray the psychiatric state of his characters as being wrought with inhibition and feelings of burden over their duties as a head of household, yet possessed a strong desire to lead an unencumbered and carefree life. This essay will attempt to analyzed the various psychiatric states of the his characters through the concepts of Freud’s psychoanalytic theory of the death drive.

스웨디시 마사지와 엔더몰러지 적용이 얼굴 부위별 수분함량과 탄력도에 미치는 영향

박주아(JuA Park),모정희(JuongHee Mo),범희주(HeeJu Beom) 한국뷰티산업학회 2012 뷰티산업연구 Vol.6 No.1

본 연구는 마사지 기법이 피부 상태와 탄력성 개선에 효과가 있는지 알아보고자 한다. 연구대상은 30대, 40대, 50대의 피부과 질환이 없는 건강한 여성을 선택하였다. 연구 방법은 얼굴의 정 중앙선을 중심으로 우측은 스웨디시 마사지 기법을 이용하고, 좌측은 엔더 몰러지를 이용하여 피부관리를 실시하였다. 임상은 2010년 8월 7일부터 10월 26까지 3개 월(12주)간 시행하였으며 대조군은 피부 관리를 받기 전 피부상태를 측정하였고 매 관리 1주일 후에 측정하였다. 피부상태 측정은 좌, 우측으로 나누어 눈 꼬리 주위와 볼 부위를 비침습적 방법에 의해 측정하였다. 피부 상태를 측정한 결과 각질층의 수분 함량은 다르게 관찰되었다. 탄력성은 연령에 관계없이 스웨디시 마사지에 비하여 엔더몰러지를 이용 한 경우에 개선 효과가 다소 높게 나타났다. This study was to observe the effects of the massage techniques on the function and elasticity of the human skin. As the experimental subjects three females were selected who were each in their 30s, 40s and 50s. All of them were healthy without any facial skin pathology. Dividing the face into the left and right sides at around the center line, facial skin care was performed by using Swedish massage technique on the right and Depressor massage technique with endermologie equipment(Skin tonicⓡ) on the left side. The experiments were performed over 12 weeks from 7th of August to 25th of October 2006. On every 7 days after the skin care with massage, the water contents and elasticity of human skin were measured by non-invasive methods at 2 points of eye rim and cheek separately. The amount of water contents of stratum corneum observed significantly different. In all ages, skin elasticity was more improved by depressor massage technique than by traditional Swedish massage.

청년 혜택 알리미 어플리케이션 설계와 구현

김주아(Jua Kim),황진성(Jinsung Hwang),박태준(Taejoon park),박은주(Eunju Park),임한규(Hankyu Lim) 한국정보기술학회 2022 Proceedings of KIIT Conference Vol.2022 No.6

청년들을 위해 국가에서 시행하는 다양한 혜택들이 많다. 자신에게 해당하는 혜택임에도 인지도가 낮아 신청하지 않는 청년들이 많다. 이에 본 논문에서는 청년들에게 정보를 제공하고 신청까지의 과정이 쉽고 빠르게 이루어지는 편리한 어플리케이션을 설계하였다. 본 논문의 어플리케이션 시스템과 UI는 시장조사와 설문조사, 벤치마킹 등을 통하여 설계하였다. 본 논문의 어플리케이션은 많은 청년들에게 도움이 될 것이라 기대한다. There are many various benefits implemented by country for young people. However, there are also many young people who do not apply due to low awareness, even though it is a benefit that corresponds to them. Therefore, in this paper, an application was designed. It is convenient to provide information to young people and to make applications easily and quickly. We designed the application system and UI through market research, surveys, and benchmarking. The application of this paper is expected to help many young people.

구매기업의 상생활동이 공급기업의 관계인지와 역량개발에 미치는 영향에 대한 실증적 연구

정주아 ( Jua Chung ),민순홍 ( Soonhong Min ),황선일 ( Sunil Hwang ) 한국로지스틱스학회 2018 로지스틱스연구 Vol.26 No.1

본 연구는 한국적 공급사슬관리의 주요 과제로 떠오른 구매기업의 상생활동에 주목하여, (1) 상생활동의 본질에 부합하는 구성개념의 하위차원성을 제안하여 상생활동 개념을 정립하고, (2) 상생활동이 공급기업의 구매기업에 대한 주요 관계인지적 요인인 ‘신뢰’와 ‘공급사슬 동일시’를 높이는지 실증하며, (3) 상생활동과 그 성과 간 관계를 공급기업의 관계인 지적 요인 (신뢰, 공급사슬 동일시)이 매개하는지 실증하여 상생활동의 효과성에 대한 연구 결과를 풍부하게 하고자 한다. 본 연구는 국내 108개 제조 기업으로부터 수집한 자료를 구조방정식 모형으로 분석하였다. 본 연구의 실증결과는 (1) 상생활동의 주요 차원성인 구매기업의 공급기업에 대한 ‘소통 및 평가의 체계화’와 ‘위험 및 보상의 공유’가 공급기업의 구매기업에 대한 ‘신뢰’를 이끌고, (2) ‘신뢰’는 구매기업 주도 공급사슬에 대한 공급기업의 ‘공급사슬 동일시’를 이끌며, (3) ‘공급사슬 동일시’는 공급기업의 ‘역량개발’을 유도함을 보여준다. 또한, 실증결과는 (4) 공급기업의‘신뢰’가 구매기업의 ‘상생활동’과 공급기업의‘공급사슬 동일시’간 관계를 매개하며, (5) ‘공급사슬 동일시’는 다시 ‘신뢰’와 ‘역량개발’간 관계를 매개함을 보여준다. 본 연구의 주요 학문적 가치는 지금까지 이론만 정립된 ‘공급사슬 동일시’라는 관계인지적 요인이 한국 특유의 공급사슬 환경에서 실존하며, ‘신뢰’와 ‘역량개발’간 관계의 매개변수임을 확인하였다는 것이다. 주요 실무적 가치는 구매기업의 상생활동이 공급기업의 행동에 직접적 영향을 미치는 개념이라기보다는 공급기업과 구매기업과의 관계를 변화시켜 궁극적으로 공급기업의 변화를 유도하는 개념임을 설명한다는 점이다. This study focuses on Korea‘s unique ’coexistence management’ concept, which has become vital to managing supply chain relationships in Korean industries. This study aims to contribute to the body of knowledge in ‘coexistence management’ in particular and supply chain management (SCM) in general by (1) theoretically suggesting several of critical dimensions of the Korea’s unique ‘coexistence management’ concept led by buying firms in consideration of the very nature of coexistence, (2) empirically testing whether buying firm’s ‘coexistence management’ help supplying firm develop relational goodwill toward buying firm --‘trust’ and ‘supply chain identity salience’, and (3) confirming if the relationship between buying firm’s ‘coexistence management’ and supplying firm’s capability development is mediated by relational goodwill. An empirical test is performed on the data collected from 108 Korean manufacturers, utilizing structural equation modeling. The test results indicate: (1) buying firm’s‘systematized communication and evaluation’ with/of supplier and ‘risk and reward sharing’with supplier , both of which are critical dimensions of coexistence management, positively affect supplying firm’s trust; (2) trust, in turn, help develop supplying firm’s supply chain identity salience; (3) supply chain identity salience then facilitates the supplying firm’ s ‘capability development’. Furthermore, (4) trust plays a mediating role in the relationship between buying firm’s coexistence management and supplying firm’s supply chain identification salience, and (5) supply chain identification salience successively mediates the trust-capability development link. A noteworthy research implication of this study is that it confirms the existence of supply chain identification salience in Korea’s unique supply chain structure ―so far it has lacked empirical evidence on the presence of that theoretical concept since it was first conceptualized in mid 2000s. A major managerial implication is that the concept of coexistence management is strategic and social in nature, rather than tactical and contractual, in that it cannot lead directly supplying firm’s strategic change (i.e., capability development); instead, coexistence management motivates supplying firm to develop its capability only via building trust and supply chain identity salience.

기업휴게시설 명상공간의 디자인 특성 연구

김주아 ( Jua Kim ),이돈일 ( Donil Lee ),황용섭 ( Yongseup Hwang ) 한국공간디자인학회 2017 한국공간디자인학회논문집 Vol.12 No.3

(Background and Purpose) Recently, the concept of rest has been clarified and the division of spaces has traditionally been distinct. In the modern working environment, the concept of rest is more complex and focused on stress responses. Meditation, a form of self-awareness of one`s own thoughts and movements, has become popular within corporate facilities and areas for meditation can be seen in corporate lounges or break rooms. This study focuses on the form of resting spaces as they change the work environment, and aims to examine the design concept and characteristics of the typical meditation space. (Method) First, this study investigates changes in design of corporate environments through a literature review before examining common features used in lounge facilities. Second, this study hopes to clarify the concept of a meditation space, determine typical characteristics of these spaces, and understand trends within this relatively new phenomenon. Third, this study collects specific examples of meditation spaces included in corporate lounges and other rest areas, analyzing the design characteristics used in these spaces. Finally, the author discusses findings and presents the importance of both concept and meaning in the designing of meditation spaces for corporate break rooms. (Result) Results of an internal and external analysis of ten different corporate meditation spaces shows that clarity is rated highest, due to ease of operation. Accessibility and convenience are also high, thanks to a successful layout that excluded unnecessary functions and inconveniences. However, diversity was the lowest rated, since the space was appropriate for a limited number of activities and opportunities, and thus failed to meet the needs of multiple groups. The meditation spaces studied also lacked any sense of heterogeneity, making it difficult to feel familiarity in these facility environments. Finally, fellowship and efficiency were low because it is often difficult to form a consensus through free communication and interaction, causing discomfort with the lack of unity. (Conclusions) Design choices made have the potential to change the work environment if meditation spaces are thoughtfully planned. Characteristics of meditation spaces should reflect its physical elements (efficiency, accessibility, clarity, convenience, diversity), psychological considerations (comfort, balance, affection, agreement, fellowship), and environmental factors. These spaces should also be sustainable and considerate of various types of users. This study suggests that providing meditation spaces in corporate lounges decreases employee reports of stress, both in regards to work stressors and interpersonal relationships, and improves work efficiency by providing an employee area that encourages mental and physically comfort. Further, increased efficiency is often linked to an increase in productivity, which can have positive effects on the local economy and lead to improvements in the welfare of neighboring communities.

논문 : 상변태 ; 금속간화합물 TiAl의 미세조직과 기계적 성질에 미치는 탄소 및 질소의 영향

윤주환 ( Jua Hwan Yun ),김민철 ( Min Chul Kim ),오명훈 ( Myung Hoon Oh ),이종탁 ( Chong Tak Lee ),위당문 ( Dang Moon Wee ) 대한금속재료학회 ( 구 대한금속학회 ) 1996 대한금속·재료학회지 Vol.34 No.11

Effects of carbon and nitrogen additions on microstructure and mechanical properties of Ti-48.5Al-1.5Mo (at.%) alloys were investigated. The alloys added with 0.3%, 1.0% and 2.0%C or N were prepared by vacuum arc melting, and heat-treated at 1390℃ for lamellar microstructure and then aged at 900℃. Tensile tests were performed in air with the strain rate of 2×10-4S-1 at room temperature and 800℃. It was found that C (or N) addition decreased the grain size of lamellar structure. Especially, 1.0%C(or N) and 2.0%C(or N) addition refined the grain size to 1/10 of that of the mother alloy. Only fine Ti3AlN precipitates were formed at the lamellar boundaries for the alloy containing 0.3% N, but Ti2AlN precipitates of a few micrometers in size were also formed in the matrix in addition to Ti3AlN precipitates for the alloys with 1.0 and 2.0% N. Tensile elongation at room temperature decreased markedly for the alloys with 0.3%C or N but recovered for the alloys with 1.0% and 2.0% addition compared to the value of the mother alloy. Yield strength at room temperature for the alloys containing 1.0% and 2.0% C or N increased up to two times of the value of the mother alloy, and the high strength was maintained up to 800℃. The aged alloys showed much higher tensile elongation at room temperature than the alloys only heat-treated to obtain the lamellar microstructure.