S-580 : Vitamin D and carotid subclinical atherosclerosis in SLE

( Chang Bum Bae ),( Ju Yang Jung ),( Bo Ram Go ),( Hyun Ah Kim ),( Chang Hee Suh ) 대한내과학회 2013 대한내과학회 추계학술대회 Vol.2013 No.1

Vitamin D and carotid subclinical atherosclerosis in SLE Chang-Bum Bae, Ju-Yang Jung, Bo-ram Go, Hyun-Ah Kim, Chang-Hee Suh Department of Rheumatology, Ajou University School of Medicine, Suwon, Korea Introduction Atherosclerosis develops earlier in systemic lupus erythematosus (SLE) and is the important cause of mortality. It has been suggested that vitamin D deficiency contributed cardiovascular disease and immune dysregulation in SLE. Methods One-hundred two female patients with SLE and 52 normal controls (NC) were recruited. The carotid artery intima-media thickness (cIMT) and plague were assessed by B-mode ultrasound at the carotid artery level. Vitamin 25(OH)D3 levels were checked by immunoradiometric assay using the Bio-Line 25(OH)D3-Ria CT kit (Bio-Line S.A., Belgium). Disease activity markers were checked at the time of enrollment. Results The cIMT of SLE is 0.41±0.08mm, which is higher than NC (0.37±0.11 mm, p=0.012). Also, carotid artery plague index (PI) of SLE is higher than NC (0.68±1.39 vs 0.26±0.87, p=0.026). The cIMT was correlated with age (r=0.442, p<0.001), body mass index (r=0.246, p=0.013), waist-hip ratio (r=0.245, p=0.013), SLE disease activity index (r=0.239, p=0.016), taking aspirin (r=0.244, p=0.013), and the carotid artery PI was correlated with renal involvement (r=0.224, p=0.023) in patients with SLE. Vitamin 25(OH)D3 was not showed correlation with cIMT and carotid artery PI. Also, it was not correlated with disease activity markers. Conclusion In patients with SLE, the risk of cardiovascular disease measured by cIMT and carotid artery plaque was higher than NC, which may be derived from systemic inflammation. However, vitamin D levels were not showed significant correlations with carotid subclinical atherosclerosis. It may be not suitable to assess vitamin D as marker of disease activity or subclinical atherosclerosis.

출류(朮類)한약재의 외,내부형태와 이화학패턴 연구

김정훈 ( Jung Hoon Kim ),이금산 ( Geum San Lee ),최고야 ( Go Ya Choi ),황성연 ( Sung Yeoun Hwang ),김홍준 ( Hong Jun Kim ),정승일 ( Seung Il Jeong ),주영승 ( Young Sung Ju ) 대한본초학회 2009 大韓本草學會誌 Vol.24 No.2

Objectives: To determine the discriminative criteria for Atractylodes rhizomes, the experiment of external-internal characteristics and physicochemical pattern analysis were performed. Methods: External characteristics was observed using stereoscope. The sectioned materials which were covered with parffin were stained by Ju`s method. Physicochemical patterns were analyzed using HPLC/DAD. Results: 1. External shape of original plant: Atractylodes macrocephala and A. japonica had relatively long petioles and 3-5 parted leaves. A. macrocephala had big purple flowers whereas A. japonica had relatively small white flowers and pinnate bracts. A. lancea had sessile leaves and white flowers, and the end parts of degenerated stamens were bent. 2. External shape of herbal medicine: A. macrocephala which was fist-shaped rhizome had pa-pillate processes and the cross section was light gray and sulcate. A. japonica and A. lancea were connected-beady or tubercular rhizomes, and the cross sections were both yellow-colored white. However, the cross section of A. japonica was fibrous, the width of cortex was narro-wer than that of stele, and radial shape in cortex was rare, whereas the width of cortex in A. lancea was similar to that of stele in size, and radial shape in cortex was obvious. 3. Internal shape of herbal medicine: A. macrocephala and A. lancea did not have lignified fascicles in cortex. However, the vascular bundles and vessels of A. macrocephala were wedge shaped and radial arrangement, and vascular bundles were densely populated in stele whereas those of A. lancea were repeatedly arranged and thinly extended to cortex. A. japonica had lignified fascicles in cortex and the width of vascular bundles was conspicuously thick with narrow intervals. 4. Physicochemical pattern analysis: A. macrocephala and A. lancea contained atractylenolide Ⅰ and atractylenolide Ⅲ whereas A. japonica contained atractylenolide Ⅰ, atractylenolide Ⅲ, diacetyl-atractylodiol, compound-4, compound-5. The three species of Atractylodes rhizomes showed different chromatogram patterns. Conclusions: The results could be used as discriminative criteria for Atractylodes rhizomes and as fundamental materials to researches of further pattern analysis and biological reaction.

후박(厚朴)의 외,내부형태 및 이화학패턴연구

이금산 ( Guem San Lee ),김정훈 ( Jung Hoon Kim ),최고야 ( Go Ya Choi ),강대훈 ( Dae Hoon Kang ),황성연 ( Sung Yeoun Hwang ),정승일 ( Seung Il Jeong ),김홍준 ( Hong Jun Kim ),주영승 ( Young Sung Ju ) 대한본초학회 2008 大韓本草學會誌 Vol.23 No.4

Objectives: To determine the standards for discrimination of Magnoliae Cortex, the experiment of specific external-internal characters and the physicochemical pattern analysis were performed. Methods: External characteristics was observed using a stereoscope. Paraffin-mediated sectioned materials were stained by Ju`s method. Physicochemical patterns of materials were analyzed using HPLC. Results: 1. Botanical characteristics: Magnolia officinalis had one seed and a white flower, while M. obovata had two seeds and a white flower. Machilus thunbergii had berry and spherical fruits and yellowish green panicles. 2. External characteristics: M. officinalis and M. obovata were dark and thick. M. officinalis was gray brown and greasy while M. obovata was light-gray, less oily and smoothly sectioned. Machilus thunbergii was thin and relatively light or yellow-brown, coarsely sectioned and faintly specific scents. 3. Internal characteristics: The bast parts of M. officinalis and M. obovata were commonly wider than Machilus thunbergii. The cork cortex of M. officinalis was 10~mg/L cell layers with many oil cells, while that of M. obovata was 4~7 cell layers with less oil cells. Machilus thunbergiis xylem which consisted of ring-shaped cambium at 1st and 2nd part was occupied in large portion. 4. Physicochemical pattern: Both M. officinalis and M. obovata involved honokiol and magnolol. All kinds of M. officinalis involved Magnatriol B but one kind of M. ovobata and all of Machilus thunbergii didn`t. Machilus thunbergii showed different pattern of chromatogram from that of 2 species above. Conclusions: These results could be used as standards for discrimination of Magnoliae Cortex and as the method of objectification in medicinal herbs giving the basic resource for bioactivity research.

Effect of the Red Ginseng against PM2.5-induced Pulmonary Dysfunction in BALB/C Mice

Ju Hui Kim,Jong Min Kim,Hyo Lim Lee,Min Ji Go,Tae Yoon Kim,Seung Gyum Joo,Han Su Lee,Ho Jin Heo 한국식품영양과학회 2022 한국식품영양과학회 학술대회발표집 Vol.2022 No.10

Genetic and clinical characteristics of Korean patients with neurofibromatosis type 2

Kim, Hye-ji,Seo, Go Hun,Kim, Yoon Myung,Kim, Gu-Hwan,Seo, Eul-Ju,Ra, Young-Shin,Choi, Jin-Ho,Yoo, Han-Wook,Lee, Beom Hee Korean Society of Medical Genetics and Genomics 2017 대한의학유전학회지 Vol.14 No.2

Purpose: Neurofibromatosis type 2 (NF2) is characterized by multiple tumors, including vestibular schwannoma (VS) and others affecting cranial and peripheral nerves. NF2 is caused by mutation of the NF2 gene. The mutation spectrum of NF2 has not been characterized in Korean patients. In the current study, the clinical and genetic characteristics of Korean NF2 patients were analyzed. Materials and Methods: Twenty-five unrelated Korean families were enrolled according to the Manchester criteria. Genetic analysis was performed by direct sequencing and multiplex ligation-dependent probe amplification methods using genomic DNA from peripheral lymphocytes or tumor tissues. Results: All patients had bilateral/unilateral VS and/or other cranial and peripheral nerve tumors. Two patients were familial cases and the other 24 patients were sporadic. Germline NF2 mutations were detected in peripheral lymphocytes from both familial cases, but only in 26.1% of the 23 sporadic families. Somatic mutations were also found in tumor tissues from two of the sporadic families. These somatic mutations were not found in peripheral lymphocytes. A total of 10 different mutations including 2 novel mutations were found in 40.0% of studied families. Five mutations (50.0%) were located in exon 6 of NF2, the FERM domain coding region. Conclusion: Family history was an important factor in identifying germline NF2 mutations. Further study is required to investigate whether exon 6 is a mutation hotspot in Korean NF2 patients and its correlation to phenotypic severity.

Phenothiazine Dendrimers Encapsulated 2-{2-[2-(4-Dimethylamino-phenyl)-vinyl]-6-methyl-pyran-4-ylidene}-malonoitrile Derivatives

Go Woon Kim,Min Ju Cho,Young-Jun Yu,Zee Hwan Kim,Jung-Il Jin,Dong Young Kim,Dong Hoon Choi 한국고분자학회 2006 한국고분자학회 학술대회 연구논문 초록집 Vol.2006 No.10

Robust Torque Control of DC Link Voltage Fluctuation for SynRM Considering Inductances With Magnetic Saturation

Kim, Seung-Joo,Im, Jong-Bin,Go, Sung Chul,Bae, Jae-Nam,Kim, Won-Ho,Kim, Kwang-Soo,Kim, Cherl-Jin,Lee, Ju IEEE 2010 IEEE transactions on magnetics Vol.46 No.6

<P> This paper presents the control method that inverter output keeps to linear to reference voltage of Synchronous Reluctance Motor (SynRM) using DC Link voltage Synthesis. This method is reduced torque ripple considering parameters with magnetic saturation. To estimate parameters of inductances for SynRM, the proposed method uses recursive least square (RLS) estimation algorithm. The estimated inductances are used for calculating torque and DC link voltage. The Inverter output voltage cannot be displayed to linear about inverter reference voltage if Real DC Link voltage is different from DC Link voltage of PWM amplitude. Also, the over-modulation is linearity broken if reference voltage is out of range. Torque ripple generates the vibration and noise of a motor. This paper proposes the control method so that the torque ripple decreases and the linearity of inverter output keeps using the DC Link voltage Synthesis. The proposed control method is verified by computer simulation and experiment. </P>

Preparation of GF37 for the improvement of respiratory inflammation

Ju Yeong So,Min Jung Kim,Go Woo Choi,You Jin Lee,Yun Ho Jo,Sejeong Kim,Danhui Kim,Sang Hun Lee,Een Yeong Park,Dae Seok Yoo,Jin Seong Kim,Sang Won Cho,Young Soo Kim,Hak Joo Choi,Mi Kyung Pyo 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

Panax ginseng (PG) and Perilla frutesens (PF) leaves have antioxidant and anti-inflammatory action, which can alleviate various diseases caused by fine dust. Therefore, the purpose of this study is to find the optimal ratio of ginseng and perilla leaves to maximize the anti-inflammatory action. PG and PF were extracted three times for 6 h at 70℃ with 70% ethanol and concentrated in vacuum, respectively, and then powdered by spray dryer. PG:PF were each made at different ratios to make 9 samples; CHJ1 (1:9), CHJ2 (2:8), CHJ3 (3:7), CHJ4 (4:6), CHJ5 (5:5), CHJ6 (6:4), CHJ7 (7:3), CHJ8 (8:2), CHJ9 (9:1). Each sample was measured TNF-a, IL-6, and ROS against fine dust (PM10) using MH-S cells. Among the 9 mixed samples, CHJ2 and CHJ3 inhibited the inflammation and oxidative stress induced by fine dust the best. In a confirmation experiment comparing these samples with PG and PF samples, the CHJ2 sample was finally selected because it had the best anti-inflammatory and antioxidant effects at a low concentration. Finally, a CHJ2 sample in which PG and PF were mixed in a 3:7 ratio was selected as a material for improving respiratory inflammation, and was named GF37.

AP2/EREBP Transcription Factors in Rice

Kim, Yun-Ju,Jung, Eui-Whan,Hwang, Seon-Hee,Go, Seong-Joo,Hwang, Duk-Ju The Korean Society of Plant Pathology 2004 Plant Pathology Journal Vol.20 No.1

Plants have the ability to defend themselves against pathogens by activating a series of defense responses. SA is known to be a signal molecule in plant defense responses. Nevertheles, SA is not the only one signal mediating defense responses. In addition to SA, ethylene and jasmonic acid have also been known to mediate plant defense responses against pathogens. The activation of a series of plant defense responses is known to be through varieties of transcription factors. Specially AP2/EREBP transcription factors are involved in ethylene mediated defense signaling. In this review, recent progress on AP2/EREBP transcription factors in arabidopsis, tomato and tobacco and a few of AP2/ EREBP transcription factors in rice related to biotic stresses will be discussed.

Cadmium exposure impairs porcine embryonic development by inducing oxidative stress and mitochondrial dysfunction

Min Ju Kim,Se‑Been Jeon,Hyo‑Gu Kang,Bong‑Seok Song,Bo‑Woong Sim,Sun‑Uk Kim,Pil‑Soo Jeong,Seong‑Keun Cho The Korean Society of Animal Reproduction and Biot 2024 한국동물생명공학회지 Vol.39 No.1

Background: Cadmium (Cd) is toxic heavy metal that accumulates in organisms after passing through their respiratory and digestive tracts. Although several studies have reported the toxic effects of Cd exposure on human health, its role in embryonic development during preimplantation stage remains unclear. We investigated the effects of Cd on porcine embryonic development and elucidated the mechanism. Methods: We cultured parthenogenetic embryos in media treated with 0, 20, 40, or 60 µM Cd for 6 days and evaluated the rates of cleavage and blastocyst formation. To investigate the mechanism of Cd toxicity, we examined intracellular reactive oxygen species (ROS) and glutathione (GSH) levels. Moreover, we examined mitochondrial content, membrane potential, and ROS. Results: Cleavage and blastocyst formation rates began to decrease significantly in the 40 µM Cd group compared with the control. During post-blastulation, development was significantly delayed in the Cd group. Cd exposure significantly decreased cell number and increased apoptosis rate compared with the control. Embryos exposed to Cd had significantly higher ROS and lower GSH levels, as well as lower expression of antioxidant enzymes, compared with the control. Moreover, embryos exposed to Cd exhibited a significant decrease in mitochondrial content, mitochondrial membrane potential, and expression of mitochondrial genes and an increase in mitochondrial ROS compared to the control. Conclusions: We demonstrated that Cd exposure impairs porcine embryonic development by inducing oxidative stress and mitochondrial dysfunction. Our findings provide insights into the toxicity of Cd exposure on mammalian embryonic development and highlight the importance of preventing Cd pollution.