DSCR1 interacts with FMRP and is required for spine morphogenesis and local protein synthesis : Regulatory activity of DSCR1 and FMRP

Wang, Wei,Zhu, John Z,Chang, Karen T,Min, Kyung-Tai Wiley Blackwell (John Wiley Sons) 2012 The EMBO journal Vol.31 No.18

lncRNA Epigenetic Landscape Analysis Identifies <i>EPIC1</i> as an Oncogenic lncRNA that Interacts with MYC and Promotes Cell-Cycle Progression in Cancer

Wang, Zehua,Yang, Bo,Zhang, Min,Guo, Weiwei,Wu, Zhiyuan,Wang, Yue,Jia, Lin,Li, Song,Caesar-Johnson, Samantha J.,Demchok, John A.,Felau, Ina,Kasapi, Melpomeni,Ferguson, Martin L.,Hutter, Carolyn M.,Sof Cell Press 2018 Cancer Cell Vol. No.

<P><B>Summary</B></P> <P>We characterized the epigenetic landscape of genes encoding long noncoding RNAs (lncRNAs) across 6,475 tumors and 455 cancer cell lines. In stark contrast to the CpG island hypermethylation phenotype in cancer, we observed a recurrent hypomethylation of 1,006 lncRNA genes in cancer, including <I>EPIC1</I> (epigenetically-induced lncRNA1). Overexpression of <I>EPIC1</I> is associated with poor prognosis in luminal B breast cancer patients and enhances tumor growth <I>in vitro</I> and <I>in vivo.</I> Mechanistically, <I>EPIC1</I> promotes cell-cycle progression by interacting with MYC through <I>EPIC1</I>'s 129–283 nt region. <I>EPIC1</I> knockdown reduces the occupancy of MYC to its target genes (e.g., <I>CDKN1A</I>, <I>CCNA2</I>, <I>CDC20</I>, and <I>CDC45</I>). MYC depletion abolishes <I>EPIC1</I>'s regulation of MYC target and luminal breast cancer tumorigenesis <I>in vitro</I> and <I>in vivo</I>.</P> <P><B>Highlights</B></P> <P> <UL> <LI> LncRNAs show a hypomethylation phenotype, in contrast to a CIMP phenotype in cancer </LI> <LI> <I>EPIC1</I> promotes breast tumorigenesis through regulating cancer cell-cycle progression </LI> <LI> <I>EPIC1</I> directly interacts with MYC protein through <I>EPIC1</I>'s 129–283 nt region </LI> <LI> <I>EPIC1</I> regulates MYC targets by enhancing MYC occupancy on its target promoters </LI> </UL> </P> <P><B>Graphical Abstract</B></P> <P>[DISPLAY OMISSION]</P>

미국에서의 서예교육

John. Wang(王純傑) 한국서예학회 2010 서예학연구 Vol.16 No.-

High Thermoelectric Performance of a Heterogeneous PbTe Nanocomposite

Wang, Hongchao,Hwang, Junphil,Snedaker, Matthew Loren,Kim, Il-ho,Kang, Chanyoung,Kim, Jungwon,Stucky, Galen D.,Bowers, John,Kim, Woochul American Chemical Society 2015 Chemistry of materials Vol.27 No.3

<P>In this paper, we propose a heterogeneous material for bulk thermoelectrics. By varying the quenching time of Na doped PbTe, followed by hot pressing, we synthesized heterogeneous nanocomposites, a mixture of nanodot nanocomposites and nanograined nanocomposites. It is well-known that by putting excess amounts of Na (i.e., exceeding the solubility limit) into PbTe, nanodots with sizes as small as a few nanometers can be formed. Nanograined regions with an average grain size of ca. 10 nm are observed only in materials synthesized with an extremely low quenching rate, which was achieved by using a quenching media of iced salt water and cold water. Dimensionless thermoelectric figures of merit, <I>zT</I>, of those heterogeneous nanocomposites exhibited a <I>zT</I> around 2.0 at 773 K, which is a 25% increase compared to <I>zT</I> of a homogeneous nanodot nanocomposite with the largest quenching time in our experiment, i.e. furnace cooled. The power factor increase is 5%, and the thermal conductivity reduction is 15%; thus, <I>zT</I> increase mainly comes from the thermal conductivity reduction.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/cmatex/2015/cmatex.2015.27.issue-3/cm5042138/production/images/medium/cm-2014-042138_0007.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/cm5042138'>ACS Electronic Supporting Info</A></P>

The Role of Dynamic Magnetic Resonance Imaging in Cervical Spondylotic Myelopathy

John Paul Kolcun,Lee Onn Chieng,Karthik Madhavan,Michael Y. Wang 대한척추외과학회 2017 Asian Spine Journal Vol.11 No.6

Dynamic spinal cord compression has been investigated for several years, but until the advent of open MRI, the use of dynamic MRI (dMRI) did not gain popularity. Several publications have shown that cervical cord compression is both static and dynamic. On many occasions the evaluation of cervical spondylotic myelopathy (CSM) is straightforward, but patients are frequently encountered with a significantly worse clinical examination than would be suggested by radiological images. In this paper, we present an extensive review of the literature in order to describe the importance of dMRI in various settings and applications. A detailed literature review was performed in the Medline and Pubmed databases using the terms “cervical spondylotic myelopathy”, “dynamic MRI”, “kinetic MRI”, and “myelomalalcia” for the period of 1980-2016. The study was limited to English language, human subjects, case series, retrospective studies, prospective reports, and clinical trials. Reviews, case reports, cadaveric studies, editorials, and commentaries were excluded. The literature search yielded 180 papers, 19 of which met inclusion criteria. However, each paper had evaluated results and outcomes in different ways. It was not possible to compile them for meta-analysis or pooled data evaluation. Instead, we evaluated individual studies and present them for discussion. We describe a number of parameters evaluated in 2661 total patients, including dynamic changes to spinal cord and canal dimensions, transient compression of the cord with changes in position, and the effects of position on the intervertebral disc. dMRI is a useful tool for understanding the development of CSM. It has found several applications in the diagnosis and preoperative evaluation of many patients, as well as certain congenital dysplasias and Hirayama disease. It is useful in correlating symptoms with the dynamic changes only noted on dMRI, and has reduced the incidence of misdiagnosis of myelopathy.

Minimally-Invasive versus Conventional Repair of Spondylolysis in Athletes: A Review of Outcomes and Return to Play

John Paul G. Kolcun,Lee Onn Chieng,Karthik Madhavan,Michael Y. Wang 대한척추외과학회 2017 Asian Spine Journal Vol.11 No.5

Spondylolysis from pars fracture is a common injury among young athletes, which can limit activity and cause chronic back pain. While current literature has examined the relative benefits of surgical and conservative management of these injuries, no study has yet compared outcomes between conventional direct repair of pars defects and modern minimally invasive procedures. The goals of surgery are pain resolution, return to play at previous levels of activity, and a shorter course of recovery. In this review, the authors have attempted to quantify any differences in outcome between patients treated with conventional or minimally invasive techniques. A literature search was performed of the PubMed database for relevant articles, excluding articles describing conservative management, traumatic injury, or high-grade spondylolisthesis. Articles included for review involved young athletes treated for symptomatic spondylolysis with either conventional or minimally invasive surgery. Two independent reviewers conducted the literature search and judged articles for inclusion. All studies were classified according to the North American Spine Society standards. Of the 116 results of our initial search, 16 articles were included with a total of 150 patients. Due to a paucity of operative details in older studies and inconsistencies in both clinical methods and reporting among most articles, little quantitative analysis was possible. However, patients in the minimally invasive group did have significantly higher rates of pain resolution (p <0.001). Short recovery times were also noted in this group. Both groups experienced low complication rates, and the majority of patients returned to previous levels of activity. Surgical repair of spondylolysis in young athletes is a safe and practical therapy. Current literature suggests that while conventional repair remains effective, minimally invasive procedures better clinical outcomes. We await further data to conduct a more thorough quantitative analysis of these techniques.

Influence of calcium on microbial reduction of solid phase uranium(VI)

Liu, Chongxuan,Jeon, Byong-Hun,Zachara, John M.,Wang, Zheming John Wiley & Sons 2007 Biotechnology and bioengineering Vol.97 No.6

<P>The effect of calcium on the dissolution and microbial reduction of a representative solid phase uranyl [U(VI)], sodium boltwoodite (NaUO<SUB>2</SUB>SiO<SUB>3</SUB>OH · 1.5H<SUB>2</SUB>O), was investigated to evaluate the rate-limiting step of microbial reduction of the solid phase U(VI). Microbial reduction experiments were performed in a culture of a dissimilatory metal-reducing bacterium (DMRB), Shewanella oneidensis strain MR-1, in a bicarbonate medium with lactate as electron donor at pH 6.8 buffered with PIPES. Calcium increased the rate of Na-boltwoodite dissolution and U(VI) bioavailability by increasing its solubility through the formation of a ternary aqueous calcium-uranyl-carbonate species. The ternary species, however, decreased the rates of microbial reduction of aqueous U(VI). Laser-induced fluorescence spectroscopy (LIFS) and transmission electron microscopy (TEM) collectively revealed that microbial reduction of solid phase U(VI) was a sequentially coupled process of Na-boltwoodite dissolution, U(VI) aqueous speciation, and microbial reduction of dissolved U(VI) to U(IV) that accumulated on bacterial surfaces/periplasm. Under studied experimental conditions, the overall rate of microbial reduction of solid phase U(VI) was limited by U(VI) dissolution reactions in solutions without calcium and limited by microbial reduction in solutions with calcium. Generally, the overall rate of microbial reduction of solid phase U(VI) was determined by the coupling of solid phase U(VI) dissolution, U(VI) aqueous speciation, and microbial reduction of dissolved U(VI) that were all affected by calcium. Biotechnol. Bioneg. 2007;97: 1415–1422. © 2007 Wiley Periodicals, Inc.</P>

The influence of suture materials on the biomechanical behavior of suture-meniscal specimens: a comparative study in a porcine model

( John Reza Matthews ),( Jiefei Wang ),( Jiwei Zhao ),( Melissa A. Kluczynski ),( Leslie J. Bisson ) 대한슬관절학회 2020 대한슬관절학회지 Vol.32 No.-

Background: Repair of a meniscal tear is indicated in certain conditions. Despite extensive research on the biomechanics of various repair methods, there has been minimal investigation of whether the suture material influences the meniscal-suture construct. The purpose of this study was to compare the biomechanical properties of nine different suture materials under cyclic and load-to-failure conditions. Methods: Ninety porcine menisci were randomly allocated to simple suture placement using either Ultrabraid^®, Ultratape^{^®}, Magnum Wire^®, TigerWire^®, TigerTape^®, LabralTape^®, Orthocord^®, 0 FiberWire^®, or 2-0 FiberWire^®. Each suture-meniscus specimen underwent cyclic loading followed by load-to-failure testing. Elongation, maximum load to failure, stiffness, and mode of failure were recorded and compared between each suture type using nonparametric testing. Mean ± standard deviation was reported and the statistical significance was p < 0.05. Results: Elongation during cyclic loading was lowest with 2-0 FiberWire (0.95 ± 0.17 mm); this value was statistically significantly different than the results for all other sutures except 0 FiberWire^® (1.09 ± 0.17 mm, p = 0.79), TigerWire^® (1.09 ± 0.29 mm, p = 0.85), TigerTape^® (1.39 ± 0.29 mm, p = 0.08), and LabralTape^® (1.20 ± 0.33 mm, p = 0.41). The highest elongation was seen with Ultrabraid^® (1.91 ± 0.34 mm); this value was statistically significantly greater than the results for all other suture materials except Orthocord^® (1.59mm± 0.31 mm, p = 0.46) and Magnum Wire^® (1.43 ± 0.25 mm, p = 0.14). Load to failure was highest for TigerTape^® (287.43 ± 41.15 N), and this result was statistically significantly different than the results for all other sutures except LabralTape^® (271.34 ± 48.48 N, p = 0.99) and TigerWire^® (251.03 ± 25.8 N, p = 0.51). Stiffness was highest for LabralTape^® (195.77 ± 49.06 N/mm), and this result was statistically significantly different than the results for all other sutures except TigerWire^® (186.49 ± 19.83 N/ mm, p = 0.45) and TigerTape^® (173.35 ± 15.60 N/mm, p = 0.19). The majority of sutures failed by pullout (n = 46, 51%) or tearing (n = 40, 45%). Conclusion: Suture design and material affect the biomechanical behavior of porcine meniscal-suture specimens. LabralTape^®, TigerWire^®, and TigerTape^® demonstrated better overall combinations of low elongation, high maximum load to failure, and high stiffness.

Low frequency noise characterizations of ZnO nanowire field effect transistors

Wang, Wenyong,Xiong, Hao D.,Edelstein, Monica D.,Gundlach, David,Suehle, John S.,Richter, Curt A.,Hong, Woong-Ki,Lee, Takhee American Institute of Physics 2007 Journal of Applied Physics Vol.101 No.4

Drinking Water with Red Beetroot Food Color Antagonizes Esophageal Carcinogenesis in N-Nitrosomethylbenzylamine-Treated Rats

John F. Lechner,Li-Shu Wang,Claudio M. Rocha,Bethany Larue,Cassandra Henry,Colleen M. McIntyre,Kenneth M. Riedl,Steven J. Schwartz,Gary D. Stoner 한국식품영양과학회 2010 Journal of medicinal food Vol.13 No.3

This study was undertaken to determine if the oral consumption of red beetroot food color would result in an inhibition of N-nitrosomethylbenzylamine (NMBA)-induced tumors in the rat esophagus. Rats were treated with NMBA and given either regular water ad libitum or water containing 78μg/mL commercial red beetroot dye, E162. The number of NMBA-induced esophageal papillomas was reduced by 45% (P<.001) in animals that received the food color compared to controls. The treatment also resulted in reduced rates of cell proliferation in both precancerous esophageal lesions and in papillomas of NMBA-treated rats, as measured by immunohistochemical staining of Ki-67 in esophageal tissue specimens. The effects of beetroot food color on angiogenesis (microvessel density by CD34 immunostaining), inflammation (by CD45 immunostaining), and apoptosis (by terminal deoxynucleotidyl transferase dUTP nick end-labeling staining) in esophageal tissue specimens were also determined. Compared to rats treated with NMBA only, the levels of angiogenesis and inflammation in the beetroot color-consuming animals were reduced, and the apoptotic rate was increased. Thus, the mechanism(s) of chemoprevention by the active constituents of red beetroot color include reducing cell proliferation, angiogenesis, and inflammation and stimulating apoptosis. Importantly, consumption of the dye in the drinking water for a period of 35 weeks did not appear to induce any overt toxicity. Based on the fact that red beetroot color contains betanins, which have strong antioxidant activity, it is postulated that these effects are mediated through inhibition of oxygen radical-induced signal transduction. However, the sum of constituents of E162 has not been determined, and other components with other mechanisms may also be involved in antagonizing cancer development.