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- 저자 : Jinyan Zhou (검색결과 3 건)
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Zhijun Zhou,Bei Guan,Jinyan Chai,Xuting Che 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.2
We used Illumina next-generation sequencing data to generate the nearly complete mitochondrial genomes (mitogenomes) of three phasmatodeans (Megalophasma granulata, Calvisia medogensis, and Phyllium tibetense) without the aid of additional techniques such as long-range PCR or cloning. The most surprising finding was the presence of a novel gene arrangement “trnR-trnA-trnN-trnSAGN-trnE-trnF” (genes underlined are encoded by the minority strand) that was detected in M. granulata. The ancestral order of this tRNA gene cluster is typically “trnA-trnR-trnN-trnSAGN-trnE-trnF”. However, trnA was inserted between trnR and trnN in M. granulata, and this represents a rare case of gene rearrangement in Phasmatodea. Phylogenetic analyses were conducted on the concatenated nucleotide sequences of all protein-coding genes and two ribosomal RNA genes. Both maximum likelihood and Bayesian analyses failed to support the monophyly of Areolatae, Anareolatae, Diapheromeridae, Phasmatidae, Heteropterygidae, Necrosciinae, and Necrosciini. However, the monophyly of several lower taxonomic groups were confirmed in our analysis. For instance, two Ramulus species and Entoria okinawaensis were recovered in the monophyletic Clitumnini, and Phraortes, Phyllium, and Bacillus species formed individual monophyletic clades. Our results support the hypothesis that wing-loss independently occurred several times in Verophasmatodea.
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Effects of puerarin on the Akt signaling pathway in bovine preadipocyte differentiation
Yun, Jinyan,Yu, Yongsheng,Zhou, Guoli,Luo, Xiaotong,Jin, Haiguo,Zhao, Yumin,Cao, Yang Asian Australasian Association of Animal Productio 2020 Animal Bioscience Vol.33 No.1
Objective: Puerarin has the potential of regulating the differentiation of preadipocytes, but its mechanism of action has not yet been elucidated. Adipocytes found in adipose tissue, the main endocrine organ, are the main sites of lipid deposition, and are widely used as a cell model in the study of in vitro fat deposition. This study aimed to investigate the effects of puerarin on adipogenesis in vitro. Methods: Puerarin was added to the culture medium during the process of adipogenesis. The proliferation and differentiation of bovine preadipocytes was measured through cell viability and staining with oil red O. The content of triacylglycerol was measured using a triglyceride assay kit. The mRNA and protein expression levels of adipogenic genes, peroxisome proliferator-activated receptor-γ (PPARγ) and CCAAT/enhancer-binding protein-α, were measured using quantitative real-time polymerase chain reaction and western blotting, respectively. Results: The addition of puerarin significantly increased adipogenesis of bovine preadipocytes and enhanced the mRNA and protein level expression of PPARγ (p<0.01). The expression of P-Akt increased after adipogenic hormonal induction, whereas puerarin significantly increased PPARγ expression by promoting the Akt signaling component, P-Akt. The mechanism of adipogenesis was found to be related to the phosphorylation level of Ser473, which may activate the downstream signaling of the Akt pathway. Conclusion: Puerarin was able to promote the differentiation of preadipocytes and improve fat deposition in cattle. The mechanism of adipogenesis was found to be related to the phosphorylation level of Ser473.
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Yanli Ren,Jinyan Zhou,Xiaoyong Zhang,Zhidong Li,Juan Zhong,Jie Yang,Tan Xu,Hong Tan 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.6
A high performance capillary electrophoresis (HPCE) method was presented to identify and quantitate free amino acids during fermentation by Bacillus subtilis. Amino acids, pre-column derivatized with phenylisothicyanate,were separated and characterized by HPCE. In order to optimize separation conditions, the assay was developed by varying the β-cyclodextrin concentration and pH of the background electrolyte. A buffer system comprising 30 mM phosphate and 3 mM β-cyclodextrin at pH 7.0, voltage of 20 kV and detection wavelength of 254 nm showed the best results, with 17 out of 20phenylthioncarbamyl amino acids in a solution adequately separated. For quantification, p-aminobenzoic acid was added as an internal standard. Analysis of free amino acids in Bacillus subtilis culture medium using this method revealed good consistency with the values obtained using conventional ninhydrin-based amino acid analyzer. Four free amino acids (aspartic acid, glutamic acid, proline, and tyrosine) concentration in an extracellular matrix during fermentation by Bacillus subtilis were mainly monitored using this method.
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