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Profiling of differentially expressed genes in human stem cells by cDNA microarray.
Kim, Chul Geun,Lee, Jong Joo,Jung, Dae Young,Jeon, Jinseon,Heo, Hyen Seok,Kang, Ho Chul,Shin, June Ho,Cho, Yoon Shin,Cha, Kyung Joon,Kim, Chan Gil,Do, Byung-Rok,Kim, Kyung Suk,Kim, Hyun-Soo Korean Society for Molecular Biology 2006 Molecules and cells Vol.21 No.3
<P>Stem cells are unique cell populations with the ability to undergo both self-renewal and differentiation, although a wide variety of adult stem cells as well as embryonic stem cells have been identified and stem cell plasticity has recently been reported. To identify genes implicated in the control of the stem cell state as well as the characteristics of each stem cell line, we analyzed the expression profiles of genes in human embryonic, hematopoietic (CD34+ and CD133+), and mesenchymal stem cells using cDNA microarrays, and identified genes that were differentially expressed in specific stem cell populations. In particular we were able to identify potential hESC signature-like genes that encode transcription factors (TFAP2C and MYCN), an RNA binding protein (IMP-3), and a functionally uncharacterized protein (MAGEA4). The overlapping sets of 22 up-regulated and 141 down-regulated genes identified in this study of three human stem cell types may also provide insight into the developmental mechanisms common to all human stem cells. Furthermore, our comprehensive analyses of gene expression profiles in various adult stem cells may help to identify the genetic pathways involved in self-renewal as well as in multi-lineage specific differentiation.</P>
Characterization of TNNC1 as a Novel Tumor Suppressor of Lung Adenocarcinoma
Kim, Suyeon,Kim, Jaewon,Jung, Yeonjoo,Jun, Yukyung,Jung, Yeonhwa,Lee, Hee-Young,Keum, Juhee,Park, Byung Jo,Lee, Jinseon,Kim, Jhingook,Lee, Sanghyuk,Kim, Jaesang Korean Society for Molecular and Cellular Biology 2020 Molecules and cells Vol.43 No.7
In this study, we describe a novel function of TNNC1 (Troponin C1, Slow Skeletal and Cardiac Type), a component of actin-bound troponin, as a tumor suppressor of lung adenocarcinoma (LUAD). First, the expression of TNNC1 was strongly down-regulated in cancer tissues compared to matched normal lung tissues, and down-regulation of TNNC1 was shown to be strongly correlated with increased mortality among LUAD patients. Interestingly, TNNC1 expression was enhanced by suppression of KRAS, and ectopic expression of TNNC1 in turn inhibited KRAS<SUP>G12D</SUP>-mediated anchorage independent growth of NIH3T3 cells. Consistently, activation of KRAS pathway in LUAD patients was shown to be strongly correlated with down-regulation of TNNC1. In addition, ectopic expression of TNNC1 inhibited colony formation of multiple LUAD cell lines and induced DNA damage, cell cycle arrest and ultimately apoptosis. We further examined potential correlations between expression levels of TNNC1 and various clinical parameters and found that low-level expression is significantly associated with invasiveness of the tumor. Indeed, RNA interference-mediated down-regulation of TNNC1 led to significant enhancement of invasiveness in vitro. Collectively, our data indicate that TNNC1 has a novel function as a tumor suppressor and is targeted for down-regulation by KRAS pathway during the carcinogenesis of LUAD.
Evaluation of Fretting Wear on Spent Nuclear Fuel Rods During Transport
Jinseon Kim,Youngik Yoo,Joongjin Kim,Joonkyoo Park,Hyeongkoo Kim 한국방사성폐기물학회 2023 한국방사성폐기물학회 학술논문요약집 Vol.21 No.1
Flow-induced vibration can lead to fretting wear damage of fuel rods and spacer grids in nuclear reactors. Similarly, during the transport of spent nuclear fuel assemblies, continuous vibration and intermittent impact might also result in fretting wear due to dynamic interaction. Therefore, it is important to evaluate fuel rod damage due to fretting wear under such transport conditions. This study examines spent nuclear fuel rod specimens fabricated with hydride cladding tubes and simulated pellets, with hydrogen content ranging from 200 to 700 ppm and oxide film thickness ranging from 25 to 100 micrometers. Tests were conducted under a worst-case scenario, assuming continuous exposure to that condition during the expected transport time. Results indicate that the wear depth of all rod specimens occurred within the oxide film, suggesting a high resistance to fretting wear during transportation.
Kim, Sung-Hyun,Choe, Chungyoul,Shin, Yong-Sung,Jeon, Mi-Jin,Choi, So-Jung,Lee, Jinseon,Bae, Gab-Yong,Cha, Hyuk-Jin,Kim, Jhingook Potamitis Press 2013 Anticancer research Vol.33 No.5
<P>The metastatic potential of non-small cell lung cancer (NSCLC) cells has been shown to be associated with the tumor microenvironment. Cancer-associated fibroblasts (CAFs) are a major component of the tumor microenvironment, regulating tumor cell function by secreting growth factors, chemokines, and extracellular matrix (ECM). In this study, we examined the role of CAFs in the tumor progression of NSCLC. Firstly, we established primary cultures of CAFs and matched normal fibroblasts (NFs) from patients with resected NSCLC. CAFs exhibited greater expression of the pan-mesenchymal marker α-smooth muscle actin (α-SMA) than did NFs, although they displayed similar morphology. Furthermore, we employed a direct co-culture assay with human NSCLC A549 and H358 cells, and found that CAFs were more potent in inducing the epithelial-to-mesenchymal transition (EMT) phenotype than NFs, as indicated by an elongated and disseminated appearance. CAF-induced EMT led to an increase in motility and a decrease in proliferation of NSCLC cells through SMAD family number-3 (SMAD3)-dependent up-regulation of the growth inhibitory gene p21(CIP1) [cyclin-dependent kinase inhibitor-1A (CDKN1A)] and α-SMA. Taken together, these findings provide evidence that lung CAFs have tumor-promoting capacity distinct from NFs and might play a significant role in the metastatic potential of NSCLC.</P>
경관법과 도시재생법의 연계방안 - 경관법과 도시재생 활성화 및 지원에 관한 특별법을 중심으로 -
Jinseon Kim 전북대학교 휴양및경관계획연구소 2015 휴양및경관연구 (J East Asian Landscape Studies) Vol.9 No.4
본 연구에서는 최근 개정된 경관법과 도시재생법의 상호연계방안을 모색하고자 하며, 특히 실행력이 약한 경관법의 단점을 도시재생법과의 상호연계를 통해 경관계획의 실효성을 높일 수 있는 방안을 모색해 보고자 한다. 문헌연구를 통해 최근 개정된 경관관련 법⋅제도와 도시재생관련 법⋅제도의 주요 내용과 다른 법과 경관법 간의 관계, 경관법과 도시재생법 간의 관계를 살펴보았다. 사례연구에서는 미국의 메인스트리트 프로그램과 BID를 선정하고 경관과 연관성이 많은 프로그램을 중심으로 고찰 하고 한국의 도시재생 선도지역 중 창원시와 순천시를 선정하여 도시재생활성화 계획내용에 대해서 경관과의 관련성을 고찰한다. 문헌연구와 사례연구를 통해서 경관법과 도시재생법의 연계 필요성과 연계방안을 도출하였다. 경관법과 도시재생법 의 주요 연계방안으로는 경관법에 의해 도시재생사업의 경관심의를 가능하도록 하는 방안, 경관법에 의한 경관계획과 도시재생법에 의한 전략계획 및 활성화 계획 수립 시 상호 연계하는 방안, 도시재생 활성화 지역으로 선정된 지역의 경관협정을 유도하는 방안이 도출되었다. This study tried to seek a method of mutual linkage between the landscape act and the urban regeneration act recently revised. In particular, this study attempted to seek a method to increase the effectiveness of landscape - planning by figuring out the shortcomings of the landscape act, which may be poorly executed through mutual linkage with the urban renewal act. A literature review was conducted to analyze the main content of recently revised landscape - related acts and institutions, the relationship between other acts and the landscape act, and the relationship between the landscape act and the urban regeneration act. In the case study, the main street program and the BID in the United States were selected and discussed with a focus on a program highly associated with a landscape. Subsequently, the case study selected Changwon and Suncheon among Korea's leading urban regeneration areas and discussed the relationship with landscapes regarding the content of urban renewal planning and activation. A literature review and case study derived the necessary linkage and method between the landscape act and the urban regeneration act. For the main linkage methods between the landscape act and the urban regeneration act, a method that allows urban regeneration projects for landscape deliberation by the landscape, a multi - linkage method in establishing landscape planning based on the landscape act, and the strategic and promotion planning based on the urban generation act, and a method to induce the landscape agreement of the region selected for promotion of urban regeneration promotion.
( Jinseon Kim ),( Woohyok Chang ),( Min Sagong ) 대한안과학회 2010 Korean Journal of Ophthalmology Vol.24 No.4
We present a case of bilateral serous retinal detachment (SRD) as a presenting sign of Philadelphia chromosome-positive acute lymphoblastic leukemia (Ph+ALL). A 45-year-old woman presented with decreased vision and was found to have bilateral serous retinal detachment. Peripheral blood smears revealed leukocytosis of 53.9x103/?L with 64.6% lymphoblasts. A bone marrow aspirate revealed the presence of lymphoblasts. Cytogenetic and molecular genetic analysis detected a reciprocal translocation between chromosome 9 and 22, t(9;22) (q34;q11). A diagnosis of Ph+ ALL was made. Following systemic chemotherapy, the bilateral SRD resolved completely with full recovery of vision. The sudden appearance of SRD should raise suspicion for leukemia. Prompt recognition of this disease is important for early systemic treatment and restoration of visual function.
Kim, Sang-Hui,Choi, Hye Young,Lee, Jinseon,Son, Dae-Soon,Lee, Hye-Sook,Song, In Seung,Lim, Yu Sung,Hong, Young-Sook,Kim, Jhingook,Choi, Yong Soo Wiley Subscription Services, Inc., A Wiley Company 2007 Journal of surgical oncology Vol.95 No.4
<B>Background and Objectives</B><P>We wanted to assess whether the level of enzyme activity for a particular matrix metalloproteinase (MMP), and not the amount of expressed protein, in lung tissue could be used as a reliable prognostic biomarker for tumor recurrence leading to poorer survival in a certain subgroup of patients who have undergone curative resection for stage I human NSCLC.</P><B>Methods</B><P>We determined what type of MMP was significant for tumor recurrence by using a mouse model of pulmonary metastasis with inoculating the footpad with H460 human cancer cells. We then looked for any association between tumor recurrence and the level of enzyme activities for the selected MMP in the tumor and also in the pathologically non-tumorous tissues from 34 stage I lung cancer patients.</P><B>Results</B><P>We obtained H460/PM6 cells having a highly metastatic potential after six repeated cycles of pulmonary metastasis by using the mouse footpad inoculated with the metastasized cancer cells in the previous cycle. We started with human lung cancer cells, H460, and we found that among the tested MMPs we tested for, the level of MMP-2 mRNA was elevated. No significant difference was seen in the level of enzyme activity of the MMP-2 cells from the curatively resected tumor tissues of the stage I NSCLC patients who were later found with or without recurrence. However, the level of MMP-2 enzyme activity was found to be significantly different between the non-tumorous lung tissues from patients later found with and without recurrence, and it was associated with the 5-year survival rate.</P><B>Conclusions</B><P>This observation suggests that the higher level of MMP-2 enzyme activity in the non-tumorous tissues from the patients could be used as a prognostic biomarker to predict post-operative tumor recurrence and survival for patients with stage I NSCLC. The elevated enzyme activity of MMP-2 in the non-tumorous tissue resected from stage I NSCLC could be used as a prognostic indicator for post-operative tumor recurrence and the patients' poor survival. Further, this could be an important aid for physicians' making decision on whether to subject particular patients to post-operative adjunct chemotherapy. J. Surg. Oncol. 2007;95:337–346. © 2007 Wiley-Liss, Inc.</P>