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Jianzeng Liu,Xiaohao Xu,Jingyuan Zhou,Guang Sun,Zhenzhuo Li,Lu Zhai,Jing Wang,Rui Ma,Daqing Zhao,Rui Jiang,Liwei Sun 고려인삼학회 2023 Journal of Ginseng Research Vol.47 No.6
Background: Our previous investigation indicated that the preparation of Panax ginseng Meyer(P. ginseng) inhibited melanogenesis. It comprised salicylic acid (SA), protocatechuic acid (PA), p-coumaricacid (p-CA), vanillic acid (VA), and caffeic acid (CA). In this investigation, the regulatory effects ofP. ginseng phenolic acid monomers on melanin production were assessed. Methods: In vitro and in vivo impact of phenolic acid monomers were assessed. Results: SA, PA, p-CA and VA inhibited tyrosinase (TYR) to reduce melanin production, whereas CA hadthe opposite effects. SA, PA, p-CA and VA significantly downregulated the melanocortin 1 receptor(MC1R), cycle AMP (cAMP), protein kinase A (PKA), cycle AMP-response element-binding protein (CREB),microphthalmia-associated transcription factor (MITF) pathway, reducing mRNA and protein levels ofTYR, tyrosinase-related protein 1 (TYRP1), and TYRP2. Moreover, CA treatment enhanced the cAMP, PKA,and CREB pathways to promote MITF mRNA level and phosphorylation. It also alleviated MITF proteinlevel in a-MSH-stimulated B16F10 cells, comparable to untreated B16F10, increasing the expression ofphosphorylation glycogen synthase kinase 3b (p-GSK3b), b-catenin, p-ERK/ERK, and p-p38/p38. Furthermore, the GSK3b inhibitor promoted p-GSK3b and p-MITF expression, as observed in CA-treatedcells. Moreover, p38 and ERK inhibitors inhibited CA-stimulated p-p38/p38, p-ERK/ERK, and p-MITFincrease, which had negative binding energies with MC1R, as depicted by molecular docking. Conclusion: P. ginseng roots' phenolic acid monomers can safely inhibit melanin production by bidirectionallyregulating melanin synthase transcription. Furthermore, they reduced MITF expression viaMC1R/cAMP/PKA signaling pathway and enhanced MITF post-translational modification via Wnt/mitogen-activated protein kinase signaling pathway.
Liao Hongdong,Wen Xiangyu,Deng Xuelei,Wu Yonghong,Xu Jianping,Li Xin,Zhou Shudong,Li Xuefeng,Zhu Chunhui,Luo Feng,Ma Yanqing,Zheng Jingyuan 한국미생물학회 2022 The journal of microbiology Vol.60 No.5
Infection by Sclerotium rolfsii will cause serious disease and lead to significant economic losses in chili pepper. In this study, the response of pepper during S. rolfsii infection was explored by electron microscopy, physiological determination and integrated proteome and metabolome analyses. Our results showed that the stomata of pepper stems were important portals for S. rolfsii infection. The plant cell morphology was significantly changed at the time of the fungal hyphae just contacting (T1) or surrounding (T2) the pepper. The chlorophyll, carotenoid, and MDA contents and the activities of POD, SOD, and CAT were markedly upregulated at T1 and T2. Approximately 4129 proteins and 823 metabolites were clearly identified in proteome and metabolome analyses, respectively. A change in 396 proteins and 54 metabolites in pepper stem tissues was observed at T1 compared with 438 proteins and 53 metabolites at T2. The proteins and metabolites related to photosynthesis and antioxidant systems in chloroplasts and mitochondria were disproportionally affected by S. rolfsii infection, impacting carbohydrate and amino acid metabolism. This study provided new insights into the response mechanism in pepper stems during S. rolfsii infection, which can guide future work on fungal disease resistance breeding in pepper.
Ultrafast epitaxial growth of metre-sized single-crystal graphene on industrial Cu foil
Xu, Xiaozhi,Zhang, Zhihong,Dong, Jichen,Yi, Ding,Niu, Jingjing,Wu, Muhong,Lin, Li,Yin, Rongkang,Li, Mingqiang,Zhou, Jingyuan,Wang, Shaoxin,Sun, Junliang,Duan, Xiaojie,Gao, Peng,Jiang, Ying,Wu, Xiaoson Elsevier 2017 Science bulletin Vol.62 No.15